LOCUS       NM_009006               4639 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus mitogen-activated protein kinase kinase kinase kinase
            2 (Map4k2), transcript variant 1, mRNA.
ACCESSION   NM_009006 XM_006531761
VERSION     NM_009006.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ren,M., Zeng,J., De Lemos-Chiarandini,C., Rosenfeld,M., Adesnik,M.
            and Sabatini,D.D.
  TITLE     In its active form, the GTP-binding protein rab8 interacts with a
            stress-activated protein kinase
  JOURNAL   Proc Natl Acad Sci U S A 93 (10), 5151-5155 (1996)
   PUBMED   8643544
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127556.4.
            
            On Jan 3, 2023 this sequence version replaced NM_009006.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: U50595.1, SRR13422601.373332.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-177               AC127556.4         183557-183733
            178-235             AC127556.4         183847-183904
            236-326             AC127556.4         184132-184222
            327-391             AC127556.4         184291-184355
            392-447             AC127556.4         184643-184698
            448-495             AC127556.4         184998-185045
            496-538             AC127556.4         185132-185174
            539-611             AC127556.4         185492-185564
            612-743             AC127556.4         185643-185774
            744-806             AC127556.4         185849-185911
            807-888             AC127556.4         186190-186271
            889-996             AC127556.4         186360-186467
            997-1075            AC127556.4         186795-186873
            1076-1134           AC127556.4         186978-187036
            1135-1178           AC127556.4         187707-187750
            1179-1217           AC127556.4         187832-187870
            1218-1251           AC127556.4         187965-187998
            1252-1360           AC127556.4         188116-188224
            1361-1449           AC127556.4         188304-188392
            1450-1491           AC127556.4         188472-188513
            1492-1576           AC127556.4         188597-188681
            1577-1656           AC127556.4         188776-188855
            1657-1717           AC127556.4         188933-188993
            1718-1835           AC127556.4         189080-189197
            1836-1894           AC127556.4         193568-193626
            1895-1998           AC127556.4         193703-193806
            1999-2176           AC127556.4         193885-194062
            2177-2245           AC127556.4         194972-195040
            2246-2332           AC127556.4         195163-195249
            2333-2403           AC127556.4         195508-195578
            2404-2459           AC127556.4         195666-195721
            2460-4639           AC127556.4         195828-198007
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 4.46 cM"
     gene            1..4639
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /note="mitogen-activated protein kinase kinase kinase
                     kinase 2"
                     /db_xref="GeneID:26412"
                     /db_xref="MGI:MGI:1346883"
     exon            1..177
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..2547
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; MEKKK
                     2; MEK kinase kinase 2; germinal center kinase;
                     rab8-interacting protein; MAPK/ERK kinase kinase kinase 2;
                     RAB8 interacting protein"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     kinase 2 isoform 1"
                     /protein_id="NP_033032.1"
                     /db_xref="CCDS:CCDS29503.1"
                     /db_xref="GeneID:26412"
                     /db_xref="MGI:MGI:1346883"
                     /translation="MALLRDVSLQDPRDRFELLQRVGAGTYGDVYKARDTVTSELAAV
                     KIVKLDPGDDISSLQQEITILRECRHPNVVAYIGSYLRNDRLWICMEFCGGGSLQEIY
                     HATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGE
                     LTASVAKRRSFIGTPYWMAPEVAAVERKGGYNELCDVWALGITAIELGELQPPLFHLH
                     PMRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTAERLLQHPFTTQH
                     LPPALLTQLLDKASDPHLGTLSPEDSELETHDMFPDTIHSRSHHGPAERTPSEIQFHQ
                     VKFGAPRRKETDPLNEPWEEEWTLLGKEELSGSLLQSVQEALEERSLTIRPALELQEL
                     DSPDDAIGTIKRAPFLGLPHTESTSGDNAQSCSPGTLSAPPAGPGSPALLPTAWATLK
                     QQEDRERSSCHGLPPTPKVHMGACFSKVFNGCPLQIHAAVTWVHPVTRDQFLVVGAEE
                     GIYTLNLHELHEDTMEKLISQRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQ
                     HQAPLSIPTNRITQRIIPRRFALSTKIPDTKGCLQCRVVRNPYTGSTFLLAALPASLL
                     LLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPEGPGCRVLFH
                     VLPLEAGLTPDILIPPEGIPGSAQQVIQVDRDTVLVSFERCVRIVNLQGEPTAALAPE
                     LTFDFTIETVVCLQDSVLAFWSHGMQGRSLDTNEVTQEITDETRIFRVLGAHRDIILE
                     SIPTDNPGAHSNLYILTGHQSSY"
     exon            178..235
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            236..326
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            327..391
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            392..447
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            448..495
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            496..538
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            539..611
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            612..743
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            744..806
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            807..888
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            889..996
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1075
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1134
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1135..1178
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1217
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1251
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1360
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1449
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1491
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..1576
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1656
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1717
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1835
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1894
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..1998
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2176
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2245
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..2332
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2403
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2459
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..4639
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2870..2875
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /note="hexamer: ATTAAA"
     polyA_site      2889
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /note="major polyA site"
     regulatory      4614..4619
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /note="hexamer: AATAAA"
     polyA_site      4639
                     /gene="Map4k2"
                     /gene_synonym="BL44; GCK; Rab8ip"
                     /note="major polyA site"
ORIGIN      
        1 gctcgcttcg ccccgcccca ccccgcgccg ctccgcgccg gctccgcagc ttgcgcccgc
       61 cggcgggtcc ggcgccgggc catggcgctg ttgcgggacg tgtcgctgca ggatccgcgg
      121 gaccgcttcg agctgctaca gcgtgtgggg gccgggacct atggcgacgt ttacaaggcc
      181 cgtgacaccg tcacgtccga actggccgcg gtgaagatcg tcaagctaga cccaggggac
      241 gacatcagct ctcttcagca ggaaatcacc atccttcgtg aatgccgcca ccccaatgtc
      301 gtggcctaca tcggcagcta cctcaggaat gaccgattgt ggatctgtat ggagttctgt
      361 ggaggagggt cactgcagga gatttaccac gccactgggc ccctggaaga acggcagatt
      421 gcctatgttt gccgggaggc actgaagggg ctccaccacc tgcattctca ggggaaaatt
      481 caccgagaca ttaagggtgc caaccttctg ctcactctgc agggtgatgt caagctggcg
      541 gactttgggg tgtcaggcga gctgacagca tctgtggcca aaagacgttc tttcattggc
      601 actccatact ggatggcacc agaggttgcc gctgtggaac gcaagggtgg ctacaatgag
      661 ctgtgtgacg tctgggccct gggcatcact gccattgagt tgggcgagct gcaaccgcca
      721 ctgttccacc tgcaccccat gagggccttg atgctcatgt cgaaaagcag cttccagcca
      781 cccaagctaa gagataagac gcgttggacc cagaatttcc accacttcct caagctggcc
      841 ctaaccaaga accccaagaa gaggcctaca gcagagaggc ttctgcagca cccattcacg
      901 acccagcatc tccctccggc cctcctcacc cagctcctcg acaaggccag tgacccccac
      961 ctgggaaccc tctcccctga agacagtgag ctggagaccc atgacatgtt tccagatacc
     1021 atccattccc gcagccatca tggtcctgct gagaggaccc cctccgagat ccagtttcac
     1081 caagtgaaat tcggtgctcc tcgccggaag gagacagatc ctctcaatga accgtgggag
     1141 gaggagtgga cgctcctggg aaaagaggaa ctgagtggga gcctgctgca gtcagtccag
     1201 gaggccctgg aggaaaggag cctgactatc cggccagcct tagaactcca ggagctggac
     1261 tccccggatg atgccatcgg aaccatcaaa cgggctccat ttttggggct gccccacact
     1321 gagtcaacat ctggtgacaa tgcccagtcc tgctccccag gtaccctgtc agcacctcca
     1381 gctggccctg gcagccccgc attgctgcct accgcctggg ctactctgaa gcaacaggag
     1441 gatcgtgagc gatcatcctg ccatgggctt cctcccaccc ccaaggtgca catgggtgcc
     1501 tgcttctcca aggtcttcaa tggctgcccc ctgcagatcc atgctgctgt cacttgggtt
     1561 caccctgtga ctcgagacca gttcctggtg gtaggggctg aggagggcat ctataccctc
     1621 aacctgcacg aactgcacga ggacacaatg gagaagctca tctcccagcg ctgctcctgg
     1681 ctctactgtg tgaacaatgt gttgctgtcg ctctcaggga aatccacaca tatctgggcg
     1741 catgacctcc cgggcctgtt tgagcagcga aggctgcagc accaggcacc cctctccatc
     1801 cctaccaacc gcatcactca gcgcatcatc cccaggcgct ttgccctgtc caccaagatt
     1861 cctgacacca aaggctgctt gcagtgccgt gtggttcgaa acccctatac cggcagtacg
     1921 ttcctgctgg ccgccctgcc tgccagcctg cttctgctgc aatggtatga gccactgcag
     1981 aaattcctgc tgctgaagaa cttttccagt cccttgccca gcccagcggg gatgctggag
     2041 ccgctggtgc tagatgggaa ggagctgcca caggtgtgtg tgggcgccga gggacccgag
     2101 ggacccggct gtcgagtcct gttccatgtc ttgcccctgg aagctggcct gactccagac
     2161 atcctcatcc ctcctgaggg gatcccaggt tctgcacagc aggtgatcca agtggacagg
     2221 gacacagtcc tggtcagctt tgaacgctgt gtgagaattg ttaacctgca gggggaaccc
     2281 acggctgcac ttgcccctga actaaccttt gacttcacta ttgagactgt ggtgtgtctg
     2341 caggatagtg tgctggcgtt ctggagccat ggcatgcagg gccgaagcct cgataccaac
     2401 gaggtgacgc aggagataac agatgagacc aggatcttcc gagtactggg ggctcacagg
     2461 gacatcatcc tggaaagcat cccaactgat aaccccgggg cacacagcaa cctctacatc
     2521 ctcacgggtc accagagcag ctactgagct atctccccga tgacgcctag gcctcaattg
     2581 ccacctccag cctctgctgg ggttccagag ggcagaccta attctgagac gtgtttgggg
     2641 gcagagaggg gaggtagccc acccacctca gcaataactg gaccagaggg agctgtgagc
     2701 acctaccctc cctggagagc tgtccccagt acaggaggcc ctgggcagga tagactgcct
     2761 gactgatcca ttccacattt tcacttttcc tttccattct ttctgccaag aagagcctgc
     2821 cccaggttct atcctgggca acatgtattt aagagagaat tatattggta ttaaagctgg
     2881 ttggttttat cccactagtc tctctgaggt agaggagttg agccctcacc ttctctgcct
     2941 tcctctctcc ttcctctttg tctccctctt ttctgcagga catctttctg taagtacttg
     3001 gttggtctta ggtgttcacc atagaagggc cagcccacta gtgctctgtg gagtctaggc
     3061 tcaggtgaca taagccagtg gtctctgccc cagcagccct ctcttggctg tgtcaattga
     3121 gtcctctgct gtaatctggc attttatgga cttgtgcggg gggaactcta agcagagacc
     3181 tgtccagggc agccttgccc caagtaggat cttgcttgtg gtctgtggga tgctggcagc
     3241 cagccaagga gctagctacc atgtccattt ctgagaccgg caccctgtcc cggccctgtc
     3301 tactccagtg tccagtgagc agcctgtaac aatacagcga agcatcagct tacactcgag
     3361 agggggatgt gagttccttt actgttcggt cactccacac caagatacag cttagcaccc
     3421 tggaagctcg ctggcatgct tctccctgtg gcttgtgctt tgtattgtcc tcccttagtc
     3481 cccttccctg tggttccaga cactgaaggc caccgaagaa tggtggggca cacagataag
     3541 gcccggcctg ggggtggggg taagggggcg tgtggagtaa aggtccagct ctgtcaccct
     3601 ctgacattta gaacaaatgt tggcagaagg tgcctggcat gctggctgca agtacttgcc
     3661 agttgagtga agcagtggtt gggtgagggt gggtaggtga cagctcatgt gagcagaatt
     3721 gtggaccttg tggttgttac tatcctcttg actactctct tccaaagctc tgactggttt
     3781 ccagtgacca agaggtttgc ttgaccaaaa taggaagcca gaggaaggaa agggacacag
     3841 tatttgtcct cagggagaca gacccaagtc aactgaggga ccaggggaga cagggctacg
     3901 gggtaaacac ctagcttcct agccccaatt tcttgggggg ctggcagtag gggtgagagg
     3961 tgggctgcag acagtgagcc tggtggcagg agcccatcag gaaaggttta ggggtatagt
     4021 atgccaaagt gaggagcctg tgaaaggcaa gatccactga tgtttgctcc cagcaaaggt
     4081 gggtgccaca agccaaagac agggcaatcc aggactgtgc actaaaagac ataattttat
     4141 ttatgtattt gtttatagcc tttttcagat acaaaataga ctctaggcta ggctatggtg
     4201 aatgcctgtg atcccagtac tcaggaggcg gaggcaggac tgcctgtgaa ttacagtcca
     4261 gcctggtcta cagagtgaat tccaggccag ccaaggttat atgtaggcaa gactctgcct
     4321 caaaaataaa ccctgccttc tcatttgtcc tccagtccct tctagaagta tttatgatat
     4381 ttaaagaatt tgacatattt ttattcacat ggaaacattc tatatatccc tctggctttt
     4441 tgaaaataca ttctggagat ctttatcaac ctggatggtt ttttttttgt tgttttgttg
     4501 ttgttttttt ggtatagcta gtgtagggtt ttatgtgttt aggtgactaa tgttaactga
     4561 aagatcttta ttgatggaat gtatttctac attaatatca caaaccacac tgcaataaat
     4621 aactcctatg tcagagcta
//
