LOCUS       NM_177395               3396 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus mitogen-activated protein kinase kinase kinase 9
            (Map3k9), transcript variant 2, mRNA.
ACCESSION   NM_177395 XM_905232
VERSION     NM_177395.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Liu,M., Xiao,B., Zhu,Y., Chen,M., Huang,J., Guo,H. and Wang,F.
  TITLE     MicroRNA-144-3p protects against chemotherapy-induced apoptosis of
            ovarian granulosa cells and activation of primordial follicles by
            targeting MAP3K9
  JOURNAL   Eur J Med Res 28 (1), 264 (2023)
   PUBMED   37537658
  REMARK    GeneRIF: MicroRNA-144-3p protects against chemotherapy-induced
            apoptosis of ovarian granulosa cells and activation of primordial
            follicles by targeting MAP3K9.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3396)
  AUTHORS   Wajapeyee,N., Malonia,S.K., Palakurthy,R.K. and Green,M.R.
  TITLE     Oncogenic RAS directs silencing of tumor suppressor genes through
            ordered recruitment of transcriptional repressors
  JOURNAL   Genes Dev 27 (20), 2221-2226 (2013)
   PUBMED   24105743
REFERENCE   4  (bases 1 to 3396)
  AUTHORS   Craig,E.A., Stevens,M.V., Vaillancourt,R.R. and Camenisch,T.D.
  TITLE     MAP3Ks as central regulators of cell fate during development
  JOURNAL   Dev Dyn 237 (11), 3102-3114 (2008)
   PUBMED   18855897
  REMARK    Review article
REFERENCE   5  (bases 1 to 3396)
  AUTHORS   Bisson,N., Tremblay,M., Robinson,F., Kaplan,D.R., Trusko,S.P. and
            Moss,T.
  TITLE     Mice lacking both mixed-lineage kinase genes Mlk1 and Mlk2 retain a
            wild type phenotype
  JOURNAL   Cell Cycle 7 (7), 909-916 (2008)
   PUBMED   18414056
  REMARK    GeneRIF: There is extensive functional redundancy between MLK1 and
            MLK2, and the other member of the family, MLK3, none of which are
            required for survival in mouse.
REFERENCE   6  (bases 1 to 3396)
  AUTHORS   Kihara,T., Kano,F. and Murata,M.
  TITLE     Modulation of SRF-dependent gene expression by association of SPT16
            with MKL1
  JOURNAL   Exp Cell Res 314 (3), 629-637 (2008)
   PUBMED   18036521
  REMARK    GeneRIF: These results show that the expression of nucleosomal
            SRF-dependent genes, including the SMC gene, is activated by MKL1
            via activation of SRF and recruitment of the FACT complex.
REFERENCE   7  (bases 1 to 3396)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK155677.1, AK053843.1 and AC125351.3.
            
            On Mar 26, 2010 this sequence version replaced NM_177395.4.
            
            Transcript Variant: This variant (2) differs in the 3' UTR and
            coding sequence compared to variant 1. The resulting isoform (2) is
            shorter at the C-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK053843.1, SRR7652917.458895.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1145              AK155677.1         7-1151
            1146-2515           AK053843.1         987-2356
            2516-3396           AC125351.3         137995-138875       c
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 37.91 cM"
     gene            1..3396
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /note="mitogen-activated protein kinase kinase kinase 9"
                     /db_xref="GeneID:338372"
                     /db_xref="MGI:MGI:2449952"
     exon            1..681
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     CDS             297..2123
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /EC_number="2.7.11.25"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase 9
                     isoform 2"
                     /protein_id="NP_796369.2"
                     /db_xref="GeneID:338372"
                     /db_xref="MGI:MGI:2449952"
                     /translation="MESSRSLLGCLASATAAPPGDDATGAGAEEEEDEEEAAAELGSH
                     AALPYWTAVFEYEAAGEDELTLRLGDVVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSN
                     YVTPRSAFSSRCQPGAEDPSCYPPIQLLEIDFAELTLEEIIGIGGFGKVYRAFWAGDE
                     VAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLCLVMEFARG
                     GPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILILQKVENG
                     DLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWE
                     LLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFTSI
                     LDQLTTIEESGFFEMPKDSFHCLQDDWKHEIQEMFDQLRAKEKELRTWEEELTRAALQ
                     QKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPRVKKRKGKFRKSRLKLKDG
                     NRISLPSDFQHKFTVQASPTMDKRKSLISNRSSPPASPTIIPRLRAIQLTPGESSKTW
                     GRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELTSGDEG"
     exon            682..1095
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1276
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1425
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1601
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1842
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1965
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..3396
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggggagggag cggccagcgg gcggcgggca cgggcggcac gagcaggtgg gagccgccga
       61 ggggcgaggc cgcccgcggg ggcgccaggc ggccgggact gcgccgcgcc cctgtccgcg
      121 gtgcgcgctc gcggtgcgca ccgggcgtcc tcgactctcg cgcgcccagc ctgctcgggc
      181 cctacctgcg gcggccgtcg ggggcgcgga gcccccagcg ctgccgagcc gcatgaacaa
      241 taggcggcgg cggctcccgc gggcggccgc cccgcaccct atggaccagc cgctccatgg
      301 agtcctccag atcgcttctc ggctgcctgg cgagtgccac tgccgccccg ccgggggacg
      361 atgccacggg cgctggggcc gaagaggagg aggacgagga ggaggcggcg gccgagctgg
      421 gatctcacgc cgcgctgccc tactggacgg ctgtgttcga gtacgaggcg gcgggcgagg
      481 acgagctgac cctgcggctg ggcgatgtgg tagaggtgct gtccaaggac tcgcaggtgt
      541 ccggcgatga gggctggtgg accggacagc tgaaccagcg ggtgggcatc ttccccagca
      601 actacgtgac cccgcgtagc gccttctcca gccgctgcca gccgggcgcc gaggacccca
      661 gctgctaccc gcccattcag ctgttagaga ttgattttgc ggagctaacc ctggaggaga
      721 tcatcggcat tgggggcttt gggaaagttt atcgtgcttt ctgggcgggc gatgaggtgg
      781 ccgtgaaggc agctcgtcac gaccctgatg aggacatcag ccagaccata gagaacgttc
      841 gccaagaggc caagctcttt gccatgctga agcacccgaa catcattgcg ctcagagggg
      901 tgtgcctgaa ggaacccaac ctctgcttgg tcatggagtt tgctcgtgga gggcctctga
      961 acagagtatt gtctgggaag aggattcccc cggacatcct ggtgaactgg gccgtgcaga
     1021 tcgccagagg gatgaactat ctacatgatg aggcgatcgt acccatcatc caccgagacc
     1081 ttaagtccag caacatattg atcctgcaga aagtggagaa tggagacctg agtaacaaga
     1141 ttctgaagat caccgacttt gggctggcgc gggaatggca ccggaccacc aagatgagtg
     1201 cggcgggaac atacgcttgg atggcacctg aagtcatccg tgcttccatg ttttccaaag
     1261 gcagcgatgt gtggagctac ggtgtactgc tttgggagct gttgactggc gaggtgccct
     1321 tccggggcat tgatggctta gcagtggctt acggtgtggc catgaacaaa ctcgcccttc
     1381 ctatcccctc tacatgtcca gagccttttg ccaaactcat ggaagactgc tggaatcccg
     1441 acccccactc gcgcccatct ttcacgagta tcctggacca gctaacgact atagaggagt
     1501 ccggtttctt tgagatgccc aaggactcct tccactgcct gcaggacgac tggaaacatg
     1561 agattcagga gatgtttgac caactcaggg ccaaagaaaa ggagctccga acctgggaag
     1621 aggagctgac ccgggctgcg ctccagcaga agaaccagga ggagctgctc cggcgtaggg
     1681 agcaggagct ggcggagcgg gagatcgaca tcctggagcg agagctcaat atcatcatcc
     1741 accagctgtg ccaggaaaag ccccgggtga agaaacgcaa gggcaagttc aggaagagcc
     1801 ggctgaagct caaggacggc aaccgcatca gcctcccctc cgatttccag cacaagttca
     1861 cggtgcaggc ctccccgacc atggataaaa ggaagagtct gatcagcaac cggtcgagtc
     1921 ctcctgcaag ccccaccatc atccctcgcc ttcgagccat ccagttgaca cctggtgaaa
     1981 gcagtaaaac ctggggccgg agctcagttg ttccaaaaga ggaaggggag gaggaggaga
     2041 agagggcccc aaagaagaag ggccggacat ggggaccagg aacacttggg cagaaagagc
     2101 tcacatcagg agatgaaggg taagaggcag atgatgtaac tgtctctctc actcacagac
     2161 tgacacaaca ttttaaagta tgataggaaa tacttttgtt tctcttgttt attttaatat
     2221 gtttggaaaa gtatacaggt ttattgtttt attttctaag atgggctggg ctagcccaag
     2281 ctgtgttcaa gctctctgtc ttcctgcctc cacctcctga gtgccgggac tacagatgga
     2341 tcacactgct acttctaagc tcttcctgac ccttctagag agttgtccag tctcctgatc
     2401 ctgggtttcc ttgtagtcag gaattgtcaa gctctttcta taaaaggcca gatgacattt
     2461 taggcttctg ggtcttcatg gtgacaaaga aataaaaaag ctgtcccaga ttctgcgtga
     2521 cacataagtg aaggagcaca tgtggctgtg ttcctctcaa acctcacaaa gacaagcggc
     2581 aggtgggatc ggattcagtc tgctggtttg cgctcctgca ggtgttctca aagcagcaag
     2641 tagcccaaat aggatgggga tgcaggaggc tgggctgagt gggctaagac ctgaggcttc
     2701 cccataaact gtctgttcag aggaagggga ggaagagcag ggtccaaaag ggctttatcc
     2761 tgaagcagag gctggcacag gacctcagct ctggcactgc cctgggatgt actgtgccct
     2821 actggactgc cttgctactc cccaactaac cacatgttaa tgcccttcta ggcaacaggt
     2881 gccttaggtg tgctgcatcc tcacttgtag gctggaggcc tgggaagcaa attctttgca
     2941 tctctgtttt tcataggcca gggctcctga tggcacagag agggtcttgg acaagaactc
     3001 agctccacag gtagctgtag agctcatgtt ttctcagcta caaaatgggc ataagactag
     3061 tacctagctt aagggttagc tgagttcctt ttgtgaagtg catcaattag ctggctcatg
     3121 gtgaacacac ctaattacca tgctgggatg atggttcgct gccttccacg tcatctactt
     3181 aatcctctaa gagtgagcat cacggtttgt atttcttcta gagtacttcc tgctcttctg
     3241 agtgtgagga tagcaatcgc agcatcattt ccagtgactt cctgttgctc actgaatggg
     3301 gcctccaaac ctgtactaac attttatgtt caaactctcc attcttcatg tctgagctca
     3361 aggggaaaaa ataacaatta gatttattaa aaaaaa
//
