LOCUS       NM_001174107           10619 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus mitogen-activated protein kinase kinase kinase 9
            (Map3k9), transcript variant 1, mRNA.
ACCESSION   NM_001174107
VERSION     NM_001174107.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   Liu,M., Xiao,B., Zhu,Y., Chen,M., Huang,J., Guo,H. and Wang,F.
  TITLE     MicroRNA-144-3p protects against chemotherapy-induced apoptosis of
            ovarian granulosa cells and activation of primordial follicles by
            targeting MAP3K9
  JOURNAL   Eur J Med Res 28 (1), 264 (2023)
   PUBMED   37537658
  REMARK    GeneRIF: MicroRNA-144-3p protects against chemotherapy-induced
            apoptosis of ovarian granulosa cells and activation of primordial
            follicles by targeting MAP3K9.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10619)
  AUTHORS   Wajapeyee,N., Malonia,S.K., Palakurthy,R.K. and Green,M.R.
  TITLE     Oncogenic RAS directs silencing of tumor suppressor genes through
            ordered recruitment of transcriptional repressors
  JOURNAL   Genes Dev 27 (20), 2221-2226 (2013)
   PUBMED   24105743
REFERENCE   4  (bases 1 to 10619)
  AUTHORS   Craig,E.A., Stevens,M.V., Vaillancourt,R.R. and Camenisch,T.D.
  TITLE     MAP3Ks as central regulators of cell fate during development
  JOURNAL   Dev Dyn 237 (11), 3102-3114 (2008)
   PUBMED   18855897
  REMARK    Review article
REFERENCE   5  (bases 1 to 10619)
  AUTHORS   Bisson,N., Tremblay,M., Robinson,F., Kaplan,D.R., Trusko,S.P. and
            Moss,T.
  TITLE     Mice lacking both mixed-lineage kinase genes Mlk1 and Mlk2 retain a
            wild type phenotype
  JOURNAL   Cell Cycle 7 (7), 909-916 (2008)
   PUBMED   18414056
  REMARK    GeneRIF: There is extensive functional redundancy between MLK1 and
            MLK2, and the other member of the family, MLK3, none of which are
            required for survival in mouse.
REFERENCE   6  (bases 1 to 10619)
  AUTHORS   Kihara,T., Kano,F. and Murata,M.
  TITLE     Modulation of SRF-dependent gene expression by association of SPT16
            with MKL1
  JOURNAL   Exp Cell Res 314 (3), 629-637 (2008)
   PUBMED   18036521
  REMARK    GeneRIF: These results show that the expression of nucleosomal
            SRF-dependent genes, including the SMC gene, is activated by MKL1
            via activation of SRF and recruitment of the FACT complex.
REFERENCE   7  (bases 1 to 10619)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK155677.1, AK053843.1 and AC125351.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155677.1, SRR17253012.2474331.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1145              AK155677.1         7-1151
            1146-2119           AK053843.1         987-1960
            2120-3958           AK155677.1         2126-3964
            3959-10619          AC125351.3         124539-131199       c
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 37.91 cM"
     gene            1..10619
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /note="mitogen-activated protein kinase kinase kinase 9"
                     /db_xref="GeneID:338372"
                     /db_xref="MGI:MGI:2449952"
     exon            1..681
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     CDS             297..3530
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /EC_number="2.7.11.25"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase 9
                     isoform 1"
                     /protein_id="NP_001167578.1"
                     /db_xref="CCDS:CCDS49104.1"
                     /db_xref="GeneID:338372"
                     /db_xref="MGI:MGI:2449952"
                     /translation="MESSRSLLGCLASATAAPPGDDATGAGAEEEEDEEEAAAELGSH
                     AALPYWTAVFEYEAAGEDELTLRLGDVVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSN
                     YVTPRSAFSSRCQPGAEDPSCYPPIQLLEIDFAELTLEEIIGIGGFGKVYRAFWAGDE
                     VAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLCLVMEFARG
                     GPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILILQKVENG
                     DLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWE
                     LLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFTSI
                     LDQLTTIEESGFFEMPKDSFHCLQDDWKHEIQEMFDQLRAKEKELRTWEEELTRAALQ
                     QKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPRVKKRKGKFRKSRLKLKDG
                     NRISLPSDFQHKFTVQASPTMDKRKSLISNRSSPPASPTIIPRLRAIQLTPGESSKTW
                     GRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELTSGDEGLKSLVDGYKQWSSSAP
                     NLGKGPRSSPALPGFTSLMEIEDEDSEGPGSGENHQQHSPNQSYLCIPFPRGEDGDGP
                     SSDGVHEEPTPVNSATSTPQLTPTNSLKRGGTHHRRCEVALLGCGAVLAATGLGFDLL
                     EAGKCQLLPPEEPEPPAREEKKRREGLFQRASRPRRSTSPPSRKLFKKEEPMTLLGDP
                     SASLTLLSLSSISECNSTRSLLRSDSDEIVVYEMPVSPVEAPPLTQCTHNPLVNVRVE
                     RFKRDPNQSLTPTHVTLTAPTQPSGHRRTPSDGALKPTAAPAVLGSRSPSSNGMSPSP
                     GTGMLKTPSPSRDPGEFPRLPDPNVVFPPTPRRWNTQRDSTLERPKTLEFLPRPRPSA
                     NRQRLDPWWFVSPSHARSASPANSSSTETPSNLDSCFASSSSTVEERPGLPALLPLQA
                     GPLLPAERTLLDLDAEGQSQDSTVPLCRAELNAHGPSPYEIQQEFWS"
     exon            682..1095
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1276
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1425
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1601
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1842
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1965
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2119
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2232
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..3045
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     exon            3046..10619
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3962
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /note="major polyA site"
     polyA_site      3986
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /note="major polyA site"
     regulatory      10591..10596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /note="hexamer: AATAAA"
     polyA_site      10612
                     /gene="Map3k9"
                     /gene_synonym="E130314H24Rik; Mlk1; Prke1"
                     /note="major polyA site"
ORIGIN      
        1 ggggagggag cggccagcgg gcggcgggca cgggcggcac gagcaggtgg gagccgccga
       61 ggggcgaggc cgcccgcggg ggcgccaggc ggccgggact gcgccgcgcc cctgtccgcg
      121 gtgcgcgctc gcggtgcgca ccgggcgtcc tcgactctcg cgcgcccagc ctgctcgggc
      181 cctacctgcg gcggccgtcg ggggcgcgga gcccccagcg ctgccgagcc gcatgaacaa
      241 taggcggcgg cggctcccgc gggcggccgc cccgcaccct atggaccagc cgctccatgg
      301 agtcctccag atcgcttctc ggctgcctgg cgagtgccac tgccgccccg ccgggggacg
      361 atgccacggg cgctggggcc gaagaggagg aggacgagga ggaggcggcg gccgagctgg
      421 gatctcacgc cgcgctgccc tactggacgg ctgtgttcga gtacgaggcg gcgggcgagg
      481 acgagctgac cctgcggctg ggcgatgtgg tagaggtgct gtccaaggac tcgcaggtgt
      541 ccggcgatga gggctggtgg accggacagc tgaaccagcg ggtgggcatc ttccccagca
      601 actacgtgac cccgcgtagc gccttctcca gccgctgcca gccgggcgcc gaggacccca
      661 gctgctaccc gcccattcag ctgttagaga ttgattttgc ggagctaacc ctggaggaga
      721 tcatcggcat tgggggcttt gggaaagttt atcgtgcttt ctgggcgggc gatgaggtgg
      781 ccgtgaaggc agctcgtcac gaccctgatg aggacatcag ccagaccata gagaacgttc
      841 gccaagaggc caagctcttt gccatgctga agcacccgaa catcattgcg ctcagagggg
      901 tgtgcctgaa ggaacccaac ctctgcttgg tcatggagtt tgctcgtgga gggcctctga
      961 acagagtatt gtctgggaag aggattcccc cggacatcct ggtgaactgg gccgtgcaga
     1021 tcgccagagg gatgaactat ctacatgatg aggcgatcgt acccatcatc caccgagacc
     1081 ttaagtccag caacatattg atcctgcaga aagtggagaa tggagacctg agtaacaaga
     1141 ttctgaagat caccgacttt gggctggcgc gggaatggca ccggaccacc aagatgagtg
     1201 cggcgggaac atacgcttgg atggcacctg aagtcatccg tgcttccatg ttttccaaag
     1261 gcagcgatgt gtggagctac ggtgtactgc tttgggagct gttgactggc gaggtgccct
     1321 tccggggcat tgatggctta gcagtggctt acggtgtggc catgaacaaa ctcgcccttc
     1381 ctatcccctc tacatgtcca gagccttttg ccaaactcat ggaagactgc tggaatcccg
     1441 acccccactc gcgcccatct ttcacgagta tcctggacca gctaacgact atagaggagt
     1501 ccggtttctt tgagatgccc aaggactcct tccactgcct gcaggacgac tggaaacatg
     1561 agattcagga gatgtttgac caactcaggg ccaaagaaaa ggagctccga acctgggaag
     1621 aggagctgac ccgggctgcg ctccagcaga agaaccagga ggagctgctc cggcgtaggg
     1681 agcaggagct ggcggagcgg gagatcgaca tcctggagcg agagctcaat atcatcatcc
     1741 accagctgtg ccaggaaaag ccccgggtga agaaacgcaa gggcaagttc aggaagagcc
     1801 ggctgaagct caaggacggc aaccgcatca gcctcccctc cgatttccag cacaagttca
     1861 cggtgcaggc ctccccgacc atggataaaa ggaagagtct gatcagcaac cggtcgagtc
     1921 ctcctgcaag ccccaccatc atccctcgcc ttcgagccat ccagttgaca cctggtgaaa
     1981 gcagtaaaac ctggggccgg agctcagttg ttccaaaaga ggaaggggag gaggaggaga
     2041 agagggcccc aaagaagaag ggccggacat ggggaccagg aacacttggg cagaaagagc
     2101 tcacatcagg agatgaaggc ctcaagtccc tggtagatgg atacaagcag tggtcatcca
     2161 gtgcccccaa cctggggaag ggcccaagga gtagccccgc cctacctggg ttcaccagcc
     2221 ttatggagat agaggatgag gatagcgaag gcccaggaag cggagagaat catcaacagc
     2281 attcacccaa ccagtcctac ctctgtatcc cgtttcctcg tggagaggat ggggatggcc
     2341 cctctagtga tggagttcat gaggaaccta ccccagtcaa ctcagctacc agtacccctc
     2401 agctgacgcc aaccaacagc ctcaagcgcg gtgggaccca ccaccggcgc tgtgaggtgg
     2461 ctctgctcgg atgtggagct gttctggcag ccacaggcct agggtttgac ttgctggaag
     2521 ctggcaagtg ccagctgctt cccccagagg aacctgagcc accagcccgg gaggagaaga
     2581 agaggcgtga aggtcttttc caaagggcca gccgccctcg tcggagtacc agccccccct
     2641 cccgaaagct cttcaagaag gaagagccaa tgacgttgct aggagacccc tctgcctcct
     2701 tgacactgct gtctctctcc tccatctctg aatgcaactc tacccgctcc ctactgcgct
     2761 ctgacagtga tgagatcgtg gtgtatgaaa tgccagtcag cccagttgaa gctccacccc
     2821 taacccaatg tacccacaac cccctggtta atgtccgagt ggagcgcttc aagagagacc
     2881 ccaaccagtc cttgactccc acccatgtca ccctcacagc ccccacgcag cctagtggtc
     2941 acaggcggac tccttctgat ggggctctta agccaacagc agcccctgca gtactaggca
     3001 gcaggagccc ctccagcaat ggaatgagtc ccagtcctgg aacaggcatg ttgaaaactc
     3061 ccagtcccag ccgagaccca ggtgaatttc cccgtctccc tgaccccaat gtggtctttc
     3121 ccccaactcc aaggcgctgg aacacccagc gagactctac cttagagaga cccaagaccc
     3181 tggagtttct gcctcgaccg cgtccttctg ccaaccggca gcgactggac ccttggtggt
     3241 ttgtgtctcc cagccatgcc cgcagcgcct ccccagctaa cagctccagc acagaaacac
     3301 ccagcaacct ggactcctgc tttgccagca gcagcagcac tgtggaagag cggcctggac
     3361 ttccagccct gctcccttta caggcaggac cactgctccc tgctgaacgg acacttttgg
     3421 acctggatgc tgaggggcag agccaggata gcactgtgcc cctatgcaga gctgagctga
     3481 atgcacacgg gccttcccca tatgagatcc aacaggagtt ctggtcttag tataaaaaag
     3541 gccagggtgg gcaagggaga agacggagga ggccgaggga gctggctggc acagcccggt
     3601 ctcaggcccc ctgggatcca gacctcctcc ttgcactgag aatgcacttt gaagatggaa
     3661 ggggtgggag cagggccact tcggagggtc tcctgccctg caggattttt tctccatgtt
     3721 cactagaggg gctgtgtcag ctctgactgt atagagaggg gtgtgcacat atgtccccac
     3781 cctccggtct tcctttcctt ttagggtgac actacagagt cattcagcca aatccgtcta
     3841 ctgcctctcc tcctcagcca gccatagctg tcctgggttc cctttgtcag ccctgagtcc
     3901 tacctgttca cacatctgta ctgatgttct gtgatctgac ttatcgtcca ctcttcccac
     3961 aactcagtgt cacctttggt atttgatgag tgtctcgtgt cctaagtcgt cccgtgccag
     4021 ctgatgggga taggctggcc ttatgaaggc cagagggctt gacagatcag gtactgccta
     4081 cctgtgcaaa tctcagtggg aactgagagt tcagaggagg aggagaggag aggaggcagt
     4141 caggaacggt ttagtcaggc tgcgtgggga ggtagagtca ggccttggtt tggtttgggt
     4201 ttaagtttta gaaacaaagg cagaaatcag cacctatggc gtggcgtggg ttcagatcat
     4261 gcttacacca gatttgaata taagcagatt catctcagga ctatctgttt gaaagacaca
     4321 ctcactgacc ccagttctga agtcaggaac aatgcctgca tttctctgtc ttgttgagct
     4381 tccattcctc cctccggttc ctgggcttta ggtcatatgt gattcgcaga gacacctgaa
     4441 ttatgtcaat agcatatggg gctgggtcct ggaccatgag gtatggaact atggggattt
     4501 cttcattgga ctgtccattt tgtgatgtcc ctgaagtaaa cggaaccctt agtaatgttt
     4561 gggtgccgtg aggcagtagc atgatacagt tcctgaggcg ctcacaccat ctctgtctct
     4621 atacacagag agcctttgtg ttgggtcctg caccatgagc tgggtagcaa gagaccagag
     4681 aggctagtgc tgacgtattt aatatttgtt tcttaaactt gaatatggag cttcaaagtg
     4741 ttttcaccca ttttctttgc tgtccattcc ctctgagccc ccggtgttgt gggtgacagt
     4801 aacaacttgg ttgaggttac agtccccagt ggtgttggag tctgtctact ggctctcttg
     4861 tgtttgccgc ctggatgccc agagggttag agcggtcggt actcatggtg caggtgacag
     4921 atttcatcag tgctgtttct ctgtgaagaa catatgagat gacgttggtt cccacaagtt
     4981 ctttatgagt tcatttgccc tttgtctcca ccctgcaccc ccagtttcag acaggggatc
     5041 ggtgctgacc atgtcagcca gagttttgct ataatatctg atcttccctc ttggttctgt
     5101 gaagtgagaa tggaagaggt taactttgcc ccccatactc taacccctca gattgaagag
     5161 gggagctcgt gaaccccatt gtcccagcca acccagtgat gatgctccac tgctccatct
     5221 gcacaccttg cctgcattaa agctttaggg taggctgcag ctggctccag cctgtgctcc
     5281 agccccaagc agggcttgac tgtagccatc tctccaggaa aggcatagtt acagatgcag
     5341 tgtacactga gtggctgcta caggccattg agtgacaggt gggatctagc ccatctccac
     5401 ccaacattcc gacagaggaa ggccttgcta aagagatgat cgtacctgag agacatgtat
     5461 actgctgcag tgagttagcg tgggttagtg tggggggagg ggtcttgttg agctccgaca
     5521 aacctgtgca gtgctggccc actgcctgcc actcttccgt gcgagcgagc ctagagatgg
     5581 ggcaagtgtt aagaagttac catttgtgtg acataggttc ccatatctgt ggtcatggtt
     5641 aggacgaggc ttggtagctg tgaatatacc cacttaagtg acagtagctt ccccctttca
     5701 cttccctttc ctcaaagaca tggctgtctg ctcggaacat gtgtccgcta ccttcaggct
     5761 gctagcagga ttggatactg ctcttttctg tgagtcttct catgccttca cttgccagtg
     5821 cttatcctta gagacccagg ctgttgctgg gaactgagga gatgcctcca tcgccacttc
     5881 cagctatttc cgggaagctg tcgtggatgc tcttcacgtg gtgttctttg acatccctgt
     5941 gaccttgtag gcatcataat aacgatcacc tacatttgaa caactcagaa cagggatgtt
     6001 ttcccttctg accttaaaag aaaagtgaca ttattttcct cacattcaat acacttgtct
     6061 caaaaacagg aagacagaca gacagacaga tgctgtttgg atggttgtgt ctggccagag
     6121 ctctgagccc ggcccgcctc ctcggttcct cttttcttgc atgactcttg tcctgtgtct
     6181 tgatccctct caggtttcct tctaaaagcc tttatcatac acttcccttg caagggcatt
     6241 gagacattgt ggctccctgc cagctcgagg gccagctcct tcccctgcac gatgaatgtg
     6301 ctgaagctga cagcatggct caccatcttt gcctcagggc atcttgcctt agttttatcc
     6361 tttggcttct ggaagatgag tgatgtggaa ttggttgaac atgattaatg ggcacccagg
     6421 ccaggcgcat gctctcccac cacttcatgt agcccaggtg actacatgat catttcctaa
     6481 ctccctcccc cacttaacct ggtcagctgg atgtccttac cattggtgtc ttactgagga
     6541 aacgggggct gggtggcctc tggtttttga ggatcatccc tagggtcata acttgctagg
     6601 aatcgaaatc aggtcagttc tggaataatt gttaggaaaa gggtccctag gcatggaaca
     6661 tcatgtgaca aagggtaata ctcctaatag aatcccttta ccctcaggct gttgctcagg
     6721 gacacaagac tttgcagggt gtctagaccc aatcctggct ccatgctgtt gccccatgag
     6781 aaggaccatt cttaactcat gaagttcatg tcttctgtgc attctcttct ttcctctgct
     6841 accccacccc aacccccata gctgcatctc ccacttgcta agcaagggcc taccaatatc
     6901 aagctctgaa cagagtaaag aacaatgggc cctaaaggct gtggctgtga gtggacacct
     6961 ccggatgtaa gtgtccaggg gtgctccacc acgggtgtag agcccccgac ttttaatcat
     7021 gtttatttga tcatgtgcct ttggatgggt gtgtaattgc gagatccgac aggtcagaag
     7081 cacacatgtc tgcctctaga aggcctggga aagaggcatg tcctagtgtt ggtgaagtac
     7141 tgaacttcct ctaaggactt gtgtagactg gaattgaggt agaggtaaga gtggccctca
     7201 gggagttgtg gtggcaagaa cctggcgact tcacctgtcc attttcaagc cgaagtttgc
     7261 cgatgtgtga ccctcagaag gcctcccgtg agctgggggt gatgtccact tctctgctta
     7321 aggccctaac tttaaaacct accatcttag ctaggttccg gggtgcattc ctagaagccc
     7381 agctacacag gaggctgagg cagggcaatt gctttagcct ggggtgtgat gtcagtctgg
     7441 ggcaacataa caaggtcctg tctcttaaat gtgtggagcc agagccccag ctccaacaca
     7501 gatggcttca ccttgcaaca ctgcttccct tggtggagaa aagtgagaat gtgaaagatg
     7561 aaaagtgagg tcaaaggcta aagttgccta ttccatatgg ctcagtcata tagcacttgt
     7621 ccttggcttg acctgagaat gacttcctgt tatgacacag ttgttgaaaa cacatttcta
     7681 gatggcaaaa cagtattttc ctagttcaaa aattgcaata aaatccgcat atagaaggag
     7741 aaagaccctt ctccttggac tctcccctgg catggatacc tggaggctca ccatctctgg
     7801 tacttagttg gagggcagga accaggagct ggaatcttgt ttgattaagg gtagtcaatc
     7861 catatctaat tagccaaacc cagacccggc ttcctggtta ggtctgcagg ccaagtgtgt
     7921 ttcttttatt ttttttcatg agctgcttct aggtattccc acccactgcc ttctctgtcc
     7981 gtttctggtt gcggaccctg gaggcacctg ggcctcccta agctgctgga gaagcgctgg
     8041 actgactgca gagttcgggg ttctcctgag ctctgcactc aggtccttga tttctttgct
     8101 gcctttgtcc ctgcatcctc tccaagtctc aagatgggaa agagaagatc ctgggattga
     8161 gagtactgac ctgagctggg agcgactttg gaaacaggcc tgccctgccc tgccctgccc
     8221 tgctctgact gacccgctaa ctgctctccg gggcatttgg gttgtcaggt gtgaaggtag
     8281 cagcaagatg gcatcccagg atggaaataa gaacacagac ttagtgttca gagagcagcc
     8341 tttcctacca ggacaggagg ggagaagccc tcaggtcaga atggctgtaa gggaaaccct
     8401 gacatgtgaa gacccctgag aagtgataca gcatgctttg tactcgctca agaccaaatt
     8461 tcaaaagttc tgtgttgccc tcgctcctgc catctttcac accaccatac ctacacagac
     8521 aaccttaggg ctgagcacca accatgccat ggatgttttt attaattttg gcattttaga
     8581 aagttatatt gaagtatggt ttagcctttg attactaatt ttttttttga cctcccattg
     8641 acatttgtat ccaatgcaag tgccttccca gcctggtatg ggataggccc tgcctaacgt
     8701 ctgcaggggc tccagatgga gctgtgctgt ttgacatagg gctttgtgtt ttatcctcat
     8761 caccacctca taccacaagt tctaagtgct gcttgtcacc ccctgggagc cctggcattc
     8821 agagacgaca gggcagcacc ctattcctat ccttggccct gtagctaggg tgacgtgtcc
     8881 tgccttcatg ttctctggct gccttttagg ctctcagaag cattccagga gaaagtggtc
     8941 ctatagagag aagcctggag attctcaact ttaaattctc tgcgaattct agaagtaaac
     9001 ggacctcatt catccgtccc gggtctcagg actcttctgc ttccctgtgt gctaagcttt
     9061 gcaaccattg tcctgtcctg tcccccccca accacccccc ccccccccgc acagcaaacc
     9121 ttcacactat cccctagggt caggctccct gccatttcct gccttcatgg tcacatcact
     9181 cacgccattt attctacctc cagttccaag gccatgtctg ctatggcttt tatttattat
     9241 ttttattatc attgttactt tggacagggt ctcatgtaac ccagacaggc ttcaaacttg
     9301 ctatatagct aaggatgacc ttggatgatc ctccacttcc cagatgctaa gattataggc
     9361 gtatagttta gtttattcct gttggaataa gataagggtt tatcctggcc cagatgtgga
     9421 gctgaacgac agattttaag aaaagttact atatcttcca cataagaagg ttctgtccag
     9481 aaatttatgt gcatatattt tgtgactcct tatacactac tccccagagt tggtgggtat
     9541 tacctgcacc caagaatgtg tggtcagact tgggggtgtt cgtactgcgt gcctagagtc
     9601 acagacctgg taagcaagtt gctgatctct ctgctctagc ctgtgagcac attcttagac
     9661 cacggttgaa aagccttaag gtgaaattca tccatctcgg gtccatgtag gttctgaaat
     9721 caccgctttc ccagggagtt gtgtcctctg gctgacctgt gatctccccg agcccagcag
     9781 acttcatcat tgcagagggt agcgttctcc ttcacctttc tcagttcggc agggattcca
     9841 acctcactgg atgacggtct gccctgtagc tcccctcctc ccagcaccga gaaggccctg
     9901 ggtctctgcc gtccgacttg gagcttcctg atacagctcc tccactctcc tccattccta
     9961 gtccttggag gttggaagcc tgtttcagct cctgtcttct agggagatca gaatgttgtc
    10021 cttttgtcgc cgtgagcctt ggcacccagc tagcaaagca ggaaggtgtt gaactcacta
    10081 atgctctgct tcccctggtc cctcctgtat ttcaccttag tccacagcca ggcctccaca
    10141 gacagcggtc tgaggctctg ttcaaggtct caaggagagg cactggacag gctctagttt
    10201 tctgaaaaag ttgtctttgg ggctttctcc tgttaggtcc cttgagtcta gaaggtacag
    10261 tgaaagttgt tttattgttc agtatttaac tacaagcgtc cttccctgca ccagtgtctc
    10321 ctctgtgtcc tgagtccctc cttgagcccc tatttgcttt ggaatttaaa ttattgtgta
    10381 ttgtcggaaa tatttaaagg cttttggaaa agtcctgaag gaagcgtatt accaggttct
    10441 ttcctttggc cttttctctt cgtgtctttc aaggtactgt acattgttga cgggatgcca
    10501 agcgggctgg gttcagtggc taagcctccc actattatag tgtcatgctg atctcagcct
    10561 ggtccccaca ccagagctct cagagacttg aataaaactg tcatactggt tactcccgg
//
