LOCUS       NM_008580               5393 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus mitogen-activated protein kinase kinase kinase 5
            (Map3k5), mRNA.
ACCESSION   NM_008580 XM_913629
VERSION     NM_008580.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Kajimoto,E., Nagasu,H., Takasu,M., Kishi,S., Wada,M., Tatsugawa,R.,
            Hirano,A., Iwakura,T., Umeno,R., Wada,Y., Itano,S., Kadoya,H.,
            Kidokoro,K., Sasaki,T. and Kashihara,N.
  TITLE     ASK-1 activation exacerbates kidney dysfunction via increment of
            glomerular permeability and accelerates cellular aging in diabetic
            kidney disease model mice
  JOURNAL   Sci Rep 14 (1), 26438 (2024)
   PUBMED   39488570
  REMARK    GeneRIF: ASK-1 activation exacerbates kidney dysfunction via
            increment of glomerular permeability and accelerates cellular aging
            in diabetic kidney disease model mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Odawara,T., Yamauchi,S. and Ichijo,H.
  TITLE     Apoptosis signal-regulating kinase 1 promotes inflammation in
            senescence and aging
  JOURNAL   Commun Biol 7 (1), 691 (2024)
   PUBMED   38839869
  REMARK    GeneRIF: Apoptosis signal-regulating kinase 1 promotes inflammation
            in senescence and aging.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5393)
  AUTHORS   He,J., Li,S., Teng,Y., Xiong,H., Wang,Z., Han,X., Gong,W. and
            Gao,Y.
  TITLE     Increasing expression of dual-specificity phosphatase 12 mitigates
            oxygen-glucose deprivation/reoxygenation-induced neuronal apoptosis
            and inflammation through inactivation of the ASK1-JNK/p38 MAPK
            pathway
  JOURNAL   Autoimmunity 57 (1), 2345919 (2024)
   PUBMED   38721693
  REMARK    GeneRIF: Increasing expression of dual-specificity phosphatase 12
            mitigates oxygen-glucose deprivation/reoxygenation-induced neuronal
            apoptosis and inflammation through inactivation of the ASK1-JNK/p38
            MAPK pathway.
REFERENCE   4  (bases 1 to 5393)
  AUTHORS   Zhao,F., Li,C., Zhuang,Y., Yan,Y., Gao,Y. and Behnisch,T.
  TITLE     Apoptosis signal-regulating kinase 1 (Ask1) deficiency alleviates
            MPP+-induced impairment of evoked dopamine release in the mouse
            hippocampus
  JOURNAL   Front Cell Neurosci 18, 1288991 (2024)
   PUBMED   38414754
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5393)
  AUTHORS   Tang,R., Mei,S., Xu,Q., Feng,J., Zhou,Y., Xing,S., He,Z. and Gao,Y.
  TITLE     ASK1-ER stress pathway-mediated fibrotic-EV release contributes to
            the interaction of alveolar epithelial cells and lung fibroblasts
            to promote mechanical ventilation-induced pulmonary fibrosis
  JOURNAL   Exp Mol Med 54 (12), 2162-2174 (2022)
   PUBMED   36473935
  REMARK    GeneRIF: ASK1-ER stress pathway-mediated fibrotic-EV release
            contributes to the interaction of alveolar epithelial cells and
            lung fibroblasts to promote mechanical ventilation-induced
            pulmonary fibrosis.
REFERENCE   6  (bases 1 to 5393)
  AUTHORS   Zama,T., Aoki,R., Kamimoto,T., Inoue,K., Ikeda,Y. and Hagiwara,M.
  TITLE     Scaffold role of a mitogen-activated protein kinase phosphatase,
            SKRP1, for the JNK signaling pathway
  JOURNAL   J Biol Chem 277 (26), 23919-23926 (2002)
   PUBMED   11959862
REFERENCE   7  (bases 1 to 5393)
  AUTHORS   Nishitoh,H., Matsuzawa,A., Tobiume,K., Saegusa,K., Takeda,K.,
            Inoue,K., Hori,S., Kakizuka,A. and Ichijo,H.
  TITLE     ASK1 is essential for endoplasmic reticulum stress-induced neuronal
            cell death triggered by expanded polyglutamine repeats
  JOURNAL   Genes Dev 16 (11), 1345-1355 (2002)
   PUBMED   12050113
REFERENCE   8  (bases 1 to 5393)
  AUTHORS   Tobiume,K., Matsuzawa,A., Takahashi,T., Nishitoh,H., Morita,K.,
            Takeda,K., Minowa,O., Miyazono,K., Noda,T. and Ichijo,H.
  TITLE     ASK1 is required for sustained activations of JNK/p38 MAP kinases
            and apoptosis
  JOURNAL   EMBO Rep 2 (3), 222-228 (2001)
   PUBMED   11266364
REFERENCE   9  (bases 1 to 5393)
  AUTHORS   Kim,A.H., Khursigara,G., Sun,X., Franke,T.F. and Chao,M.V.
  TITLE     Akt phosphorylates and negatively regulates apoptosis
            signal-regulating kinase 1
  JOURNAL   Mol Cell Biol 21 (3), 893-901 (2001)
   PUBMED   11154276
REFERENCE   10 (bases 1 to 5393)
  AUTHORS   Tobiume,K., Inage,T., Takeda,K., Enomoto,S., Miyazono,K. and
            Ichijo,H.
  TITLE     Molecular cloning and characterization of the mouse apoptosis
            signal-regulating kinase 1
  JOURNAL   Biochem Biophys Res Commun 239 (3), 905-910 (1997)
   PUBMED   9367868
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC133697.1, AC171277.9, AC158621.9, AK078668.1 and AC158620.6.
            
            On Apr 2, 2008 this sequence version replaced NM_008580.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC116627.1, AB006787.3 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-382               BC133697.1         1-382
            383-383             AC171277.9         147138-147138
            384-871             BC133697.1         384-871
            872-872             AC158621.9         35166-35166         c
            873-3376            BC133697.1         873-3376
            3377-4514           AK078668.1         1173-2310
            4515-5393           AC158620.6         101724-102602       c
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 9.35 cM"
     gene            1..5393
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /note="mitogen-activated protein kinase kinase kinase 5"
                     /db_xref="GeneID:26408"
                     /db_xref="MGI:MGI:1346876"
     exon            1..549
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..4223
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /EC_number="2.7.11.25"
                     /note="ASK-1; MEKK 5; MAPK/ERK kinase kinase 5; apoptosis
                     signal-regulating kinase 1; MEK kinase 5"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     5"
                     /protein_id="NP_032606.4"
                     /db_xref="CCDS:CCDS35857.1"
                     /db_xref="GeneID:26408"
                     /db_xref="MGI:MGI:1346876"
                     /translation="MGTEAGEGITFSVPPFASVGFCTIPEGGSCRRGGGAATAAEGEP
                     SLQPLLVPPPPPPPGSFWNVESAAAPGTSCPTTAPGSSATRGRGNSGSGGGRRTTVAY
                     VINEASQGQLVVAESEALQSLREACEAVGATLETLHFGKLDFGETAVLDRFYNADIAV
                     VEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTVCTGNYT
                     FIPYMVTPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFVQLLKVAQASSS
                     QYFRESILSDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDY
                     DSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEEQV
                     ASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAG
                     HQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHLRVIQASEKL
                     FRLKTPAWYLKSIVETILIYKHFVKLTTEQPSAKQELVDFWMDFLVEATKTDVTVVRF
                     PVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFGASSVRGVSISKF
                     EERCCFLYVLHNSDDFQIYFCTELHCKRFFEMVNTITEEKGRGAEDGDCEGDSLEYDY
                     EYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLK
                     HKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGL
                     KYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPE
                     IIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESM
                     SAEAKAFILKCFEPDPDKRACANDLLIDEFLKVSSKKKKTQPKLSALSTGSNEYLRSI
                     SLPVPVLVEDTSSSSEYGSVSPDTELKADPFSFKARAKSCGEKDGKGIRTLFLGIPDE
                     NFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKVVRNLMESLAQGAEEPK
                     LKWEHITTLISSLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVN
                     KVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADPEDLDVEDE
                     HEELSSNQTVRRPQAITEDAVATSGVSTLSSTVSHDSQNAHRSLNVQLGRMKIETNRL
                     LEELVRKERELQALLHQAIEEKDQEIRHLKLKSQPIDIPGFPVCHLNSPGTTTEDSEL
                     PGWLRENGADEDTISRFLAEDYTLVDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRN
                     KC"
     exon            550..689
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            690..713
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            714..907
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1076
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1183
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1354
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1467
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1627
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1781
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1889
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1939
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2035
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2117
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2251
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2252..2379
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2516
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2622
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2780
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2781..2958
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..2979
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            2980..3157
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            3158..3326
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            3327..3512
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            3513..3618
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            3619..3862
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            3863..3975
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            3976..4088
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            4089..4165
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     exon            4166..5393
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4922..4927
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /note="hexamer: AGTAAA"
     polyA_site      4951
                     /gene="Map3k5"
                     /gene_synonym="7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5"
                     /note="major polyA site"
ORIGIN      
        1 gcccggagct gtggctagcc agacggtgcg tctgggctgg ggactccggg gtcgccgccg
       61 tggcagcccg gcaggcagag atgggcaccg aagccggcga gggcatcact ttctccgtgc
      121 cgccctttgc gtccgtgggc ttttgcacca tcccggaggg cggcagctgc cggaggggag
      181 gaggagcggc gacggcggcg gagggagagc cgagcctgca gcccttgctt gtgccgccgc
      241 cgccgccacc gccgggcagt ttctggaacg tggagagcgc ggcggctccc ggcaccagct
      301 gccctaccac cgcccccggg agcagcgcca cccgaggccg gggcaactct ggcagcgggg
      361 gcggccggcg taccaccgtg gcttatgtga tcaatgaggc gagccagggg cagctggtgg
      421 tggcggagag cgaggccctg cagagcctgc gggaggcgtg cgaggcggtg ggcgccaccc
      481 tggagaccct gcatttcggg aagctggact tcggggaaac cgccgtgctg gaccgttttt
      541 acaacgcaga tattgctgtg gtggagatga gcgacgcctt ccggcagcct tccttgtttt
      601 accatcttgg agtgcgagaa agcttcagta tggccaacaa catcatcctc tactgcgata
      661 ctaattccga ttcactccag tccctgaagg aaattatttg ccagaagaat actgtgtgca
      721 ccgggaacta caccttcatc ccctacatgg tgacaccaca caacaaggtc tactgctgtg
      781 acagcagctt catgaagggc ctgactgagc tcatgcagcc taacttcgag ctcctgctgg
      841 ggcccatctg cttacccctc gtggaccgtt tcgttcagct tttgaaggtg gcgcaagcga
      901 gttccagcca gtacttccgg gaatccatac tcagcgatat caggaaagca cggaacctgt
      961 acactggtaa agaattggca gctgaattgg cgaggatccg gcaacgggta gacaatattg
     1021 aagttttgac agcagacatt gttatcaacc tgttgctttc ctacagagac atccaggact
     1081 atgactccat agtgaagctg gtagagacgt tagaaaaact tccaaccttc gacttggcct
     1141 cgcaccacca cgtgaagttt cattacgcat ttgcactgaa caggagaaat ctccctggag
     1201 acagagcaaa agctcttgac atcatgatcc ccatggtaca aagtgaagag caagttgctt
     1261 cggatatgta ttgtctcgtt ggccgaatct acaaagatat gtttttggat tcaaatttca
     1321 ctgacacgga aagcagggac catggagcat cttggttcaa aaaggcattt gagtctgagc
     1381 caacactaca gtcaggaatt aattatgcag ttctcttgct ggcggctggg caccagtttg
     1441 aatcttcttt tgagcttcgg aaagttggag tgaagctgag tagccttctg ggtaaaaagg
     1501 ggaacttgga aaaactccag agctactggg aagtcggatt ttttctggga gccagtgtcc
     1561 tggccaacga ccacctcagg gtcattcagg catccgagaa gctgttcaga ctgaagacac
     1621 cagcgtggta cctcaagtct atcgtggaga ccattctgat atataaacac tttgtgaaac
     1681 tgaccacaga gcagccctcg gctaagcagg aactcgtgga cttctggatg gatttcctgg
     1741 tcgaggccac aaaaacagat gttactgttg tcaggttccc agtattaata ttagagccaa
     1801 ccaaaatcta tcagccttct tacctgtcta tcaataatga agttgaggag aagacaatat
     1861 ctatttggca tgttctccct gatgacaaga aaggtataca cgagtggaac ttcggtgctt
     1921 cctctgtcag gggagtgagc atctctaagt ttgaagaacg atgctgtttc ctttatgtgc
     1981 ttcacaattc tgatgacttc caaatctact tctgcacaga acttcactgt aaaaggtttt
     2041 ttgaaatggt gaataccatc acggaagaga aggggagagg cgcggaggac ggagactgtg
     2101 agggtgactc cctggagtat gactacgaat acgatgaaaa tggagacaga gttgtgttag
     2161 ggaagggcac ttatgggatc gtctatgcag gacgagacct gagcaaccag gtcagaattg
     2221 ctattaagga aatcccagag agagacagca gatactctca gcccctgcat gaagaaatcg
     2281 ccctgcacaa gcatctgaaa cacaaaaaca ttgtccagta tctgggctcc ttcagtgaga
     2341 acggcttcat caaaatcttc atggagcaag tcccaggagg aagcctctct gctctccttc
     2401 gttcaaaatg gggcccatta aaggacaacg aacagacgat tggcttctat acgaagcaga
     2461 tactggaagg attaaaatac cttcacgaca atcagatagt ccaccgggat ataaagggtg
     2521 acaatgtatt gattaatacc tacagtggtg tgctcaagat ctctgacttc gggacatcca
     2581 agaggcttgc tggcataaac ccatgtaccg aaacctttac aggtaccctt cagtatatgg
     2641 cgccagaaat aatcgataaa ggaccacgag gctatggaaa ggccgccgac atttggtcac
     2701 tgggctgcac aatcatcgaa atggctactg gaaaaccacc attttatgaa ctaggagagc
     2761 cacaggcagc catgttcaag gtggggatgt ttaaggtcca cccggagatc ccagagtcca
     2821 tgtcggcgga ggccaaggcg ttcatactga agtgttttga accagaccct gacaagagag
     2881 cctgtgctaa tgacttgctt attgatgagt tcttaaaagt gtccagcaaa aagaaaaaga
     2941 cacaacccaa actttcagct ctctcgactg gatcgaatga gtatcttaga agcatctccc
     3001 tgccggtccc tgtcctggta gaagacacca gcagcagtag cgagtatggc tctgtttctc
     3061 ctgatacaga gctgaaggcg gaccccttct ccttcaaagc cagagctaag tcctgtggag
     3121 aaaaggacgg gaaagggata cggacattgt tcctgggcat tccagatgaa aattttgaag
     3181 atcacagtgc gcccccatct cctgaagaga aagactcggg cttttttatg ctgagaaagg
     3241 atagtgagag gcgagctacc cttcacagaa tcctgaccga agaccaggac aaggttgtaa
     3301 ggaacctaat ggaatctctg gcccagggtg ctgaagagcc taaactaaag tgggaacaca
     3361 tcacaaccct catctcgagc ctcagagagt ttgtgaggtc cactgaccga aaaatcatag
     3421 ccactacact gtccaagctc aagctggagc tggacttcga cagccacggc atcagccagg
     3481 tgcaggtggt cctctttggc ttccaggatg cggtcaataa agttcttcgg aatcataaca
     3541 tcaagccaca ctggatgttt gccctggaca gcatcatccg aaaggctgtg cagactgcaa
     3601 ttaccattct ggttccagag ttgaggccac attttagcct tgcatcagag agcgacacag
     3661 ctgatccgga agacctggat gtcgaagatg aacacgagga attgtcttca aatcagacag
     3721 tccgacggcc tcaggccatc actgaggacg ctgtggctac ctcgggggtg agcacgctaa
     3781 gctccactgt gtcccatgac tcccagaatg cacaccggtc gctgaacgtg cagcttggaa
     3841 ggatgaagat tgaaactaat aggttacttg aagagctggt tcggaaagag agagagttac
     3901 aggcactcct ccatcaagcc attgaggaaa aagaccaaga aattaggcac ctgaagctca
     3961 agtcccagcc catagatatc cctgggtttc ctgtgtgcca cctgaactct cctggcacga
     4021 ccacggaaga ttctgaactt cctggctggc tgagagaaaa tggagctgat gaagacacta
     4081 taagtcggtt tctggctgag gattacacgc tagtggatgt tctttactac gtcacacgtg
     4141 atgatctgaa gtgcctcaga ctaaggggcg ggatgctgtg cacgctgtgg aaggccatca
     4201 ttgactttcg gaacaaatgc tgaccgccac cagggtgacc ttgactggag agcctgaact
     4261 ttgaagtaga atgctctcct gtgggagggg gagccgacag gaggagaggg agaagctgca
     4321 ctttaaatcc agtctttgtt tactcgtgtc gcaaagaaag aaagaaattg acaaaacaca
     4381 cggaaacttc ctaacttctg tttctacaaa attcctacag actcttcttc acaaaggctt
     4441 tcagaataat cctgactgga gttgtaacat cggggattgc tttgtctaca cattttgacg
     4501 gagaggtttt tttttgtttt ttttttaagt cagcagctat tgtgtttcaa cccagtgttt
     4561 gtcacggaga agacagaacg tttgtttcgt gagactgcgt accactggaa caaaaccaaa
     4621 atgtgaccac ggctttttag ggttctctgt gtgtgtagtg tgcccaagtg tctgagcaga
     4681 aatgcataat ttcagtcgtt gtataaagtt ttattttttt tttaagtgtg cagagactga
     4741 gagtaatggc ttctcctctg tactgattgt cacactgact gtcttttgta acttaagttt
     4801 ctggcctagg gtgtgtttgt tgaaggggtt tatgtactac tgagcggtac cagttgccca
     4861 ttctggatca cagcggagtt agcttgttct aaagctacct gttgtgttat atttacataa
     4921 aagtaaaagg attgctggcg tgctactttg acaaagtccc gagaattctt actctgtgca
     4981 gatgcaccaa ataacttggt tagattttgt gtttgtgttt tggttttcct ctatgacatc
     5041 aactagaatg tgtctctctc tctctgttca ggcaatgaac ggaccactgg ggtcgactgt
     5101 tccctgggga cagcagggcg ggtggggggg ggacacacaa gagagttttg aatattttag
     5161 actcgatgta ttttccccat gaccctaagt taccttgaaa aagtaagaca ccgtacctta
     5221 tctcaagtgg tggacagagg atcctagtgg acaccactgc cttttctgtc tcctcaatgt
     5281 gattctcaca tctgggggaa atttttgagc caaatattat atttttatga accttcctat
     5341 gtaagcagga tgaggtctgg agctcctagg tggataaata aaattaattt cct
//
