LOCUS       NM_178084               1312 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus mitogen-activated protein kinase kinase kinase 20
            (Map3k20), transcript variant 2, mRNA.
ACCESSION   NM_178084
VERSION     NM_178084.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Vind,A.C., Wu,Z., Firdaus,M.J., Snieckute,G., Toh,G.A., Jessen,M.,
            Martinez,J.F., Haahr,P., Andersen,T.L., Blasius,M., Koh,L.F.,
            Maartensson,N.L., Common,J.E.A., Gyrd-Hansen,M., Zhong,F.L. and
            Bekker-Jensen,S.
  TITLE     The ribotoxic stress response drives acute inflammation, cell
            death, and epidermal thickening in UV-irradiated skin in vivo
  JOURNAL   Mol Cell 84 (24), 4774-4789 (2024)
   PUBMED   39591967
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Xi,J., Snieckute,G., Martinez,J.F., Arendrup,F.S.W., Asthana,A.,
            Gaughan,C., Lund,A.H., Bekker-Jensen,S. and Silverman,R.H.
  TITLE     Initiation of a ZAKalpha-dependent ribotoxic stress response by the
            innate immunity endoribonuclease RNase L
  JOURNAL   Cell Rep 43 (4), 113998 (2024)
   PUBMED   38551960
REFERENCE   3  (bases 1 to 1312)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1312)
  AUTHORS   Snieckute,G., Ryder,L., Vind,A.C., Wu,Z., Arendrup,F.S.,
            Stoneley,M., Chamois,S., Martinez-Val,A., Leleu,M., Dreos,R.,
            Russell,A., Gay,D.M., Genzor,A.V., Choi,B.S., Basse,A.L., Sass,F.,
            Dall,M., Dollet,L.C.M., Blasius,M., Willis,A.E., Lund,A.H.,
            Treebak,J.T., Olsen,J.V., Poulsen,S.S., Pownall,M.E.,
            Jensen,B.A.H., Clemmensen,C., Gerhart-Hines,Z., Gatfield,D. and
            Bekker-Jensen,S.
  TITLE     ROS-induced ribosome impairment underlies ZAKalpha-mediated
            metabolic decline in obesity and aging
  JOURNAL   Science 382 (6675), eadf3208 (2023)
   PUBMED   38060659
REFERENCE   5  (bases 1 to 1312)
  AUTHORS   Stonadge,A., Genzor,A.V., Russell,A., Hamed,M.F., Romero,N.,
            Evans,G., Pownall,M.E., Bekker-Jensen,S. and Blanco,G.
  TITLE     Myofibrillar myopathy hallmarks associated with ZAK deficiency
  JOURNAL   Hum Mol Genet 32 (17), 2751-2770 (2023)
   PUBMED   37427997
  REMARK    GeneRIF: Myofibrillar myopathy hallmarks associated with ZAK
            deficiency.
            Erratum:[Hum Mol Genet. 2023 Dec 1;32(24):3390. doi:
            10.1093/hmg/ddad168.. PMID: 37788121]
REFERENCE   6  (bases 1 to 1312)
  AUTHORS   Choi,H.S., Choi,B.Y., Cho,Y.Y., Zhu,F., Bode,A.M. and Dong,Z.
  TITLE     Phosphorylation of Ser28 in histone H3 mediated by mixed lineage
            kinase-like mitogen-activated protein triple kinase alpha
  JOURNAL   J Biol Chem 280 (14), 13545-13553 (2005)
   PUBMED   15684425
  REMARK    GeneRIF: Results show that mixed lineage kinase-like
            mitogen-activated protein triple kinase (MLTK_-alpha plays a key
            role in the UVB- and EGF-induced phosphorylation of histone H3 at
            Ser28.
REFERENCE   7  (bases 1 to 1312)
  AUTHORS   Takahashi,M., Gotoh,Y., Isagawa,T., Nishimura,T., Goyama,E.,
            Kim,H.S., Mukai,H. and Ono,Y.
  TITLE     Regulation of a mitogen-activated protein kinase kinase kinase,
            MLTK by PKN
  JOURNAL   J Biochem 133 (2), 181-187 (2003)
   PUBMED   12761180
  REMARK    GeneRIF: PKNalpha functions as not only an upstream activator of
            MLTKalpha but also a putative scaffold protein for the p38gamma
            MAPK signaling pathway
REFERENCE   8  (bases 1 to 1312)
  AUTHORS   Gotoh,I., Adachi,M. and Nishida,E.
  TITLE     Identification and characterization of a novel MAP kinase kinase
            kinase, MLTK
  JOURNAL   J Biol Chem 276 (6), 4276-4286 (2001)
   PUBMED   11042189
REFERENCE   9  (bases 1 to 1312)
  AUTHORS   Guo,W.X., Burger,A.M., Fischer,R.T., Sieckmann,D.G., Longo,D.L. and
            Kenny,J.J.
  TITLE     Sequence changes at the V-D junction of the VH1 heavy chain of
            anti-phosphocholine antibodies alter binding to and protection
            against Streptococcus pneumoniae
  JOURNAL   Int Immunol 9 (5), 665-677 (1997)
   PUBMED   9184912
REFERENCE   10 (bases 1 to 1312)
  AUTHORS   Zwacka,R.M., Reuter,A., Pfaff,E., Moll,J., Gorgas,K., Karasawa,M.
            and Weiher,H.
  TITLE     The glomerulosclerosis gene Mpv17 encodes a peroxisomal protein
            producing reactive oxygen species
  JOURNAL   EMBO J 13 (21), 5129-5134 (1994)
   PUBMED   7957077
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL928699.9.
            
            On Dec 29, 2021 this sequence version replaced NM_178084.4.
            
            Transcript Variant: This variant (2) lacks multiple 3' exon but has
            an alternate 3' segment, as compared to variant 1. The resulting
            isoform (2) is much shorter and has a distinct C-terminus, as
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045444.1, SRR11927936.3212895.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               AL928699.9         51236-51360
            126-318             AL928699.9         63693-63885
            319-406             AL928699.9         121063-121150
            407-508             AL928699.9         130133-130234
            509-574             AL928699.9         133906-133971
            575-603             AL928699.9         137232-137260
            604-741             AL928699.9         137376-137513
            742-828             AL928699.9         144139-144225
            829-903             AL928699.9         149636-149710
            904-1312            AL928699.9         154975-155383
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 43.11 cM"
     gene            1..1312
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /note="mitogen-activated protein kinase kinase kinase 20"
                     /db_xref="GeneID:65964"
                     /db_xref="MGI:MGI:2443258"
     exon            1..125
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            126..318
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..1029
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /EC_number="2.7.11.25"
                     /note="isoform 2 is encoded by transcript variant 2;
                     MLK-like mitogen-activated protein triple kinase;
                     mitogen-activated protein kinase kinase kinase MLT;
                     MLK-related kinase; mixed lineage kinase-related kinase;
                     leucine zipper- and sterile alpha motif kinase ZAK;
                     sterile alpha motif and leucine zipper containing kinase
                     AZK; human cervical cancer suppressor gene 4 protein;
                     leucine zipper- and sterile alpha motif-containing kinase"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     20 isoform 2"
                     /protein_id="NP_835185.2"
                     /db_xref="GeneID:65964"
                     /db_xref="MGI:MGI:2443258"
                     /translation="MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAV
                     KKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEM
                     DMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNH
                     TTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLV
                     VEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSF
                     LHNKAEWRWVAPA"
     exon            319..406
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            407..508
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            509..574
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            575..603
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            604..741
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            742..828
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            829..903
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1312
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1163
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /note="major polyA site"
     regulatory      1295..1300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /note="hexamer: AATATA"
     polyA_site      1312
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
ORIGIN      
        1 gtcttcctca ttggcgccgc gcggagaggc ggaatgttca actcctgact ccggcggaag
       61 cgtgggagcc gcgcgggccg ccgtccccga cccccgccgc cgctcgcgcc cgatccggga
      121 gccagatttt gtggacgttt actactttgt cattatgaga tgtcgtctct cggagcctcc
      181 tttgtgcaaa ttaagttcga cgacttgcag ttttttgaaa actgcggtgg aggaagtttt
      241 gggagtgtgt atcgagccaa atggatatca caggacaagg aggtggctgt aaagaagtta
      301 ctcaaaatag agaaagaggc agaaatcctg agcgtcctca gtcacagaaa catcatccag
      361 ttttatggag tgattctgga acctcccaac tatggcatcg tcacagaata tgcttccctg
      421 ggctccctgt atgattacat taacagcaac aggagtgaag agatggacat ggaacacatc
      481 atgacctggg ccactgacgt agccaaaggg atgcattact tacacatgga agctcctgtc
      541 aaggtgatcc acagagacct caagtcaaga aacgttgtta tcgctgctga cggagtgctg
      601 aagatctgtg acttcggtgc ctcgcggttc cataaccaca caacacacat gtccttggtt
      661 ggaactttcc catggatggc tccagaagtt atccagagtc tccctgtgtc tgaaacctgt
      721 gacacgtatt cctatggtgt ggttctctgg gagatgctaa caagggaggt cccctttaaa
      781 ggtttggaag gattacaagt agcttggctt gtagtggaaa aaaacgagag attaaccatt
      841 ccaagcagct gccccagaag ctttgctgaa ctgctacacc agtgttggga ggctgatgcc
      901 aagaaacggc catcgttcaa gcaaatcatt tcaatcctgg agtctatgtc aaatgacacg
      961 aaccttcctg accagtgtaa ctcattttta cacaacaagg cggagtggag gtgggtagct
     1021 ccggcatgag tcccggccca aggctgagga gaaccagaca cccgatggac agtcctgatg
     1081 actaagcaca gcttgggggc gcgggggatg ggacagcaag cccaagagtt ataaccccgc
     1141 attcatatgt gagagtttgc ctatgcttgt tgtcgtctat ggtcgaagtg tctgggggag
     1201 agtactgaag tcaaagccat ttccagaccc ttctctgtac acattctgtg ttgagaagct
     1261 ttggtctctt tccagaaaaa aaatatcttt ttgaaatata tatatatata ta
//
