LOCUS       NM_023057               3240 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus mitogen-activated protein kinase kinase kinase 20
            (Map3k20), transcript variant 1, mRNA.
ACCESSION   NM_023057
VERSION     NM_023057.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Vind,A.C., Wu,Z., Firdaus,M.J., Snieckute,G., Toh,G.A., Jessen,M.,
            Martinez,J.F., Haahr,P., Andersen,T.L., Blasius,M., Koh,L.F.,
            Maartensson,N.L., Common,J.E.A., Gyrd-Hansen,M., Zhong,F.L. and
            Bekker-Jensen,S.
  TITLE     The ribotoxic stress response drives acute inflammation, cell
            death, and epidermal thickening in UV-irradiated skin in vivo
  JOURNAL   Mol Cell 84 (24), 4774-4789 (2024)
   PUBMED   39591967
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Xi,J., Snieckute,G., Martinez,J.F., Arendrup,F.S.W., Asthana,A.,
            Gaughan,C., Lund,A.H., Bekker-Jensen,S. and Silverman,R.H.
  TITLE     Initiation of a ZAKalpha-dependent ribotoxic stress response by the
            innate immunity endoribonuclease RNase L
  JOURNAL   Cell Rep 43 (4), 113998 (2024)
   PUBMED   38551960
REFERENCE   3  (bases 1 to 3240)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3240)
  AUTHORS   Snieckute,G., Ryder,L., Vind,A.C., Wu,Z., Arendrup,F.S.,
            Stoneley,M., Chamois,S., Martinez-Val,A., Leleu,M., Dreos,R.,
            Russell,A., Gay,D.M., Genzor,A.V., Choi,B.S., Basse,A.L., Sass,F.,
            Dall,M., Dollet,L.C.M., Blasius,M., Willis,A.E., Lund,A.H.,
            Treebak,J.T., Olsen,J.V., Poulsen,S.S., Pownall,M.E.,
            Jensen,B.A.H., Clemmensen,C., Gerhart-Hines,Z., Gatfield,D. and
            Bekker-Jensen,S.
  TITLE     ROS-induced ribosome impairment underlies ZAKalpha-mediated
            metabolic decline in obesity and aging
  JOURNAL   Science 382 (6675), eadf3208 (2023)
   PUBMED   38060659
REFERENCE   5  (bases 1 to 3240)
  AUTHORS   Stonadge,A., Genzor,A.V., Russell,A., Hamed,M.F., Romero,N.,
            Evans,G., Pownall,M.E., Bekker-Jensen,S. and Blanco,G.
  TITLE     Myofibrillar myopathy hallmarks associated with ZAK deficiency
  JOURNAL   Hum Mol Genet 32 (17), 2751-2770 (2023)
   PUBMED   37427997
  REMARK    GeneRIF: Myofibrillar myopathy hallmarks associated with ZAK
            deficiency.
            Erratum:[Hum Mol Genet. 2023 Dec 1;32(24):3390. doi:
            10.1093/hmg/ddad168.. PMID: 37788121]
REFERENCE   6  (bases 1 to 3240)
  AUTHORS   Choi,H.S., Choi,B.Y., Cho,Y.Y., Zhu,F., Bode,A.M. and Dong,Z.
  TITLE     Phosphorylation of Ser28 in histone H3 mediated by mixed lineage
            kinase-like mitogen-activated protein triple kinase alpha
  JOURNAL   J Biol Chem 280 (14), 13545-13553 (2005)
   PUBMED   15684425
  REMARK    GeneRIF: Results show that mixed lineage kinase-like
            mitogen-activated protein triple kinase (MLTK_-alpha plays a key
            role in the UVB- and EGF-induced phosphorylation of histone H3 at
            Ser28.
REFERENCE   7  (bases 1 to 3240)
  AUTHORS   Takahashi,M., Gotoh,Y., Isagawa,T., Nishimura,T., Goyama,E.,
            Kim,H.S., Mukai,H. and Ono,Y.
  TITLE     Regulation of a mitogen-activated protein kinase kinase kinase,
            MLTK by PKN
  JOURNAL   J Biochem 133 (2), 181-187 (2003)
   PUBMED   12761180
  REMARK    GeneRIF: PKNalpha functions as not only an upstream activator of
            MLTKalpha but also a putative scaffold protein for the p38gamma
            MAPK signaling pathway
REFERENCE   8  (bases 1 to 3240)
  AUTHORS   Gotoh,I., Adachi,M. and Nishida,E.
  TITLE     Identification and characterization of a novel MAP kinase kinase
            kinase, MLTK
  JOURNAL   J Biol Chem 276 (6), 4276-4286 (2001)
   PUBMED   11042189
REFERENCE   9  (bases 1 to 3240)
  AUTHORS   Guo,W.X., Burger,A.M., Fischer,R.T., Sieckmann,D.G., Longo,D.L. and
            Kenny,J.J.
  TITLE     Sequence changes at the V-D junction of the VH1 heavy chain of
            anti-phosphocholine antibodies alter binding to and protection
            against Streptococcus pneumoniae
  JOURNAL   Int Immunol 9 (5), 665-677 (1997)
   PUBMED   9184912
REFERENCE   10 (bases 1 to 3240)
  AUTHORS   Zwacka,R.M., Reuter,A., Pfaff,E., Moll,J., Gorgas,K., Karasawa,M.
            and Weiher,H.
  TITLE     The glomerulosclerosis gene Mpv17 encodes a peroxisomal protein
            producing reactive oxygen species
  JOURNAL   EMBO J 13 (21), 5129-5134 (1994)
   PUBMED   7957077
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL928699.9 and AL845274.17.
            
            On Dec 29, 2021 this sequence version replaced NM_023057.5.
            
            Transcript Variant: This variant (1) is the longest transcript and
            encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132186.1, BC023718.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               AL928699.9         51236-51360
            126-318             AL928699.9         63693-63885
            319-406             AL928699.9         121063-121150
            407-508             AL928699.9         130133-130234
            509-574             AL928699.9         133906-133971
            575-603             AL928699.9         137232-137260
            604-741             AL928699.9         137376-137513
            742-828             AL928699.9         144139-144225
            829-903             AL928699.9         149636-149710
            904-1010            AL928699.9         154975-155081
            1011-1146           AL928699.9         163795-163930
            1147-1191           AL928699.9         175654-175698
            1192-1222           AL928699.9         177435-177465
            1223-1362           AL928699.9         177576-177715
            1363-1425           AL928699.9         182046-182108
            1426-1439           AL928699.9         183460-183473
            1440-1518           AL845274.17        2001-2079
            1519-1635           AL845274.17        17400-17516
            1636-1710           AL845274.17        19263-19337
            1711-1861           AL845274.17        22234-22384
            1862-3240           AL845274.17        25264-26642
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 43.11 cM"
     gene            1..3240
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /note="mitogen-activated protein kinase kinase kinase 20"
                     /db_xref="GeneID:65964"
                     /db_xref="MGI:MGI:2443258"
     exon            1..125
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            126..318
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..2568
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /EC_number="2.7.11.25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     MLK-like mitogen-activated protein triple kinase;
                     mitogen-activated protein kinase kinase kinase MLT;
                     MLK-related kinase; mixed lineage kinase-related kinase;
                     leucine zipper- and sterile alpha motif kinase ZAK;
                     sterile alpha motif and leucine zipper containing kinase
                     AZK; human cervical cancer suppressor gene 4 protein;
                     leucine zipper- and sterile alpha motif-containing kinase"
                     /codon_start=1
                     /product="mitogen-activated protein kinase kinase kinase
                     20 isoform 1"
                     /protein_id="NP_075544.1"
                     /db_xref="CCDS:CCDS38143.1"
                     /db_xref="GeneID:65964"
                     /db_xref="MGI:MGI:2443258"
                     /translation="MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAV
                     KKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEM
                     DMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNH
                     TTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLV
                     VEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSF
                     LHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSF
                     EIGAWTEDDVYFWVQQLVRKGESSVEMSGYASLFKENNITGKRLLLLEEEDLKDMGIV
                     SKGHIIHFKSAIEKLTHDYLNLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGT
                     GPQDCKWKMYMEMDGDEVAITYIKDVTFNTSLPDAEILKMTKPPFVMEKWIVGIAEDQ
                     TVECTVTYENDVRTPKLTKHVHSIQWDRTKPQDEVKAVQLAIQTLFSSSEGNPGSRSD
                     SSADCQWLDTLRMRQIASHTSLQRSQSNPILGSPFFPYFANQDSYAAAVRRTQTPVKY
                     QQITPSINPSRSSSPTQYGLSRNFSSLNLSSRDSGFSSLNDSSSERGRYSDRSRNKYY
                     RGSVSLNSSPKGRYGGKSQHSTPSRERYSGKFYRLPQSALNTHQSPDFKRSPNDHDRR
                     VPRTIPGMPLHPETASKAGEEESRVSEGGWTKVEYRKKTHRQLSAKTSKERTRGNYRG
                     RRNF"
     exon            319..406
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            407..508
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            509..574
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            575..603
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            604..741
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            742..828
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            829..903
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1010
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1146
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1191
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1222
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1362
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1425
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1518
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1635
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1710
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1861
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..3240
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3214..3219
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /note="hexamer: AATAAA"
     polyA_site      3240
                     /gene="Map3k20"
                     /gene_synonym="B230120H23Rik; HCCS-4; MLTK; MLTKalpha;
                     MLTKbeta; MRK; Zak"
                     /note="major polyA site"
ORIGIN      
        1 gtcttcctca ttggcgccgc gcggagaggc ggaatgttca actcctgact ccggcggaag
       61 cgtgggagcc gcgcgggccg ccgtccccga cccccgccgc cgctcgcgcc cgatccggga
      121 gccagatttt gtggacgttt actactttgt cattatgaga tgtcgtctct cggagcctcc
      181 tttgtgcaaa ttaagttcga cgacttgcag ttttttgaaa actgcggtgg aggaagtttt
      241 gggagtgtgt atcgagccaa atggatatca caggacaagg aggtggctgt aaagaagtta
      301 ctcaaaatag agaaagaggc agaaatcctg agcgtcctca gtcacagaaa catcatccag
      361 ttttatggag tgattctgga acctcccaac tatggcatcg tcacagaata tgcttccctg
      421 ggctccctgt atgattacat taacagcaac aggagtgaag agatggacat ggaacacatc
      481 atgacctggg ccactgacgt agccaaaggg atgcattact tacacatgga agctcctgtc
      541 aaggtgatcc acagagacct caagtcaaga aacgttgtta tcgctgctga cggagtgctg
      601 aagatctgtg acttcggtgc ctcgcggttc cataaccaca caacacacat gtccttggtt
      661 ggaactttcc catggatggc tccagaagtt atccagagtc tccctgtgtc tgaaacctgt
      721 gacacgtatt cctatggtgt ggttctctgg gagatgctaa caagggaggt cccctttaaa
      781 ggtttggaag gattacaagt agcttggctt gtagtggaaa aaaacgagag attaaccatt
      841 ccaagcagct gccccagaag ctttgctgaa ctgctacacc agtgttggga ggctgatgcc
      901 aagaaacggc catcgttcaa gcaaatcatt tcaatcctgg agtctatgtc aaatgacacg
      961 aaccttcctg accagtgtaa ctcattttta cacaacaagg cggagtggag gtgtgaaatt
     1021 gaggcaaccc ttgagcgact gaagaaacta gagcgagatc tcagctttaa agagcaggag
     1081 ctcaaagaac gggagagacg tctcaagatg tgggagcaga agctgacgga gcaatccaac
     1141 accccgctgc tgccttcctt tgagattggt gcgtggactg aagacgatgt gtatttttgg
     1201 gttcagcagc tcgtcagaaa aggcgagtct tcagtagaga tgagtggata cgcaagtttg
     1261 tttaaggaga acaacatcac aggcaagcgg ttgctgcttc tggaagagga agatctgaaa
     1321 gacatgggca tcgtctccaa gggacacatc attcacttta agtcagctat tgagaagcta
     1381 acccacgatt atctgaacct gtttcacttc ccaccactga ttaaggattc aggaggggaa
     1441 cctgaagaaa atgaggaaaa aatagtgaac cttgaacttg tttttggttt tcacttgaag
     1501 ccaggaactg gcccacagga ttgcaaatgg aaaatgtata tggagatgga tggggacgaa
     1561 gttgcaatca cgtacataaa agatgtgact ttcaacacga gcctccctga tgcagagatt
     1621 ttaaagatga caaagccacc atttgtaatg gagaagtgga tcgtgggaat agcggaggat
     1681 cagactgtgg agtgcacggt cacctatgag aatgatgtca gaacaccaaa actcactaag
     1741 cacgtgcact ccatacagtg ggacagaacg aagcctcagg atgaggtgaa ggcggtccag
     1801 cttgccattc agactctgtt ctccagctca gagggcaacc ctggcagcag atccgactcc
     1861 agtgcggact gccaatggtt agacactctg cgcatgcggc agattgcatc ccacacttct
     1921 ctacagcgtt cccaaagcaa ccctatcttg gggtctcctt tcttccctta ctttgccaac
     1981 caggattcct atgctgctgc ggtgaggagg acccagactc cggtgaagta ccagcagatt
     2041 acaccaagca tcaacccttc ccggagctcc tcccccacgc agtatgggct gtccagaaac
     2101 ttctcctccc tgaacctcag ctccagggac agcggcttct ccagcctcaa tgacagctcc
     2161 tccgagaggg gccgctactc agaccgaagc agaaacaagt actaccgtgg tagtgtgtcc
     2221 ctcaattctt ccccgaaagg aagatatggt gggaaaagtc agcattccac accttcaaga
     2281 gaaagatatt ctggaaagtt ctacaggctg ccccagtcag cgctgaacac tcatcagtcc
     2341 cctgacttca agaggagccc aaatgaccat gaccgccgtg tgcccaggac catacccggg
     2401 atgcctctgc accctgagac tgcctccaaa gcaggggaag aggagagcag ggtgagcgaa
     2461 gggggctgga ctaaagtgga atatcggaag aagacacaca ggcaactttc ggccaagact
     2521 agcaaggaga gaacccgtgg caactaccgt gggcggcgga atttttgagg gattgggtca
     2581 gatgcgcttt tccaagcggg ttagtagtgc tttgtgcaat ggatttttgg taataaggta
     2641 tcttgaggtt atagggacca aaaaaagact agttctgggc ttgggaaaca ccttttaact
     2701 gaaaggacaa ccaaagcagg gcctcaatta tggctgttag tcagtcaact agaggtggca
     2761 attaaacttg acaataactg ccaatgtttt gtcccctgaa ttactattga ggtggggagg
     2821 gctatgacat tgcctgctgg gaacattcga gcaggcttgg ggccaggata aagctagtga
     2881 gaaccacaga aaaatcatat ttctggacaa aacaggattc atcttatctt ctctttcatc
     2941 taagttccaa gatggctctg caaagcactg tggacgattc ttgagttaaa atttgttttt
     3001 tgattttggg gttttgtttt ttttttttta acctactggg ataaaatatc atttatcttg
     3061 attgctgagg gattttttta aaatgcctcc ctaaatgttg tgcccaaggc aagtgcctca
     3121 ctcatctcca ctgtgttagt atttatttta gtgagatatt tgtgttttct gccatggtca
     3181 gtattgaact gatttggctt gtcattttta ggtaataaaa aaaaagatta ttgaagttaa
//
