LOCUS       NM_009157               3756 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus mitogen-activated protein kinase kinase 4 (Map2k4),
            transcript variant 2, mRNA.
ACCESSION   NM_009157
VERSION     NM_009157.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Itah,Z., Chaudhry,S., Raju Ponny,S., Aydemir,O., Lee,A.,
            Cavanagh-Kyros,J., Tournier,C., Muller,W.J. and Davis,R.J.
  TITLE     HER2-driven breast cancer suppression by the JNK signaling pathway
  JOURNAL   Proc Natl Acad Sci U S A 120 (4), e2218373120 (2023)
   PUBMED   36656864
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Castro-Torres,R.D., Olloquequi,J., Etchetto,M., Caruana,P.,
            Steele,L., Leighton,K.M., Urena,J., Beas-Zarate,C., Camins,A.,
            Verdaguer,E. and Auladell,C.
  TITLE     Dual Mkk4 and Mkk7 Gene Deletion in Adult Mouse Causes an
            Impairment of Hippocampal Immature Granule Cells
  JOURNAL   Int J Mol Sci 22 (17), 9545 (2021)
   PUBMED   34502457
  REMARK    GeneRIF: Dual Mkk4 and Mkk7 Gene Deletion in Adult Mouse Causes an
            Impairment of Hippocampal Immature Granule Cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3756)
  AUTHORS   Caliz,A.D., Yoo,H.J., Vertii,A., Dolan,A.C., Tournier,C.,
            Davis,R.J., Keaney,J.F. Jr. and Kant,S.
  TITLE     Mitogen Kinase Kinase (MKK7) Controls Cytokine Production In Vitro
            and In Vivo in Mice
  JOURNAL   Int J Mol Sci 22 (17), 9364 (2021)
   PUBMED   34502275
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3756)
  AUTHORS   Preston,S.P., Doerflinger,M., Scott,H.W., Allison,C.C., Horton,M.,
            Cooney,J. and Pellegrini,M.
  TITLE     The role of MKK4 in T-cell development and immunity to viral
            infections
  JOURNAL   Immunol Cell Biol 99 (4), 428-435 (2021)
   PUBMED   33175451
  REMARK    GeneRIF: The role of MKK4 in T-cell development and immunity to
            viral infections.
REFERENCE   5  (bases 1 to 3756)
  AUTHORS   Goodwani,S., Fernandez,C., Acton,P.J., Buggia-Prevot,V.,
            McReynolds,M.L., Ma,J., Hu,C.H., Hamby,M.E., Jiang,Y., Le,K.,
            Soth,M.J., Jones,P. and Ray,W.J.
  TITLE     Dual Leucine Zipper Kinase Is Constitutively Active in the Adult
            Mouse Brain and Has Both Stress-Induced and Homeostatic Functions
  JOURNAL   Int J Mol Sci 21 (14), 4849 (2020)
   PUBMED   32659913
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3756)
  AUTHORS   Nishina,H., Fischer,K.D., Radvanyi,L., Shahinian,A., Hakem,R.,
            Rubie,E.A., Bernstein,A., Mak,T.W., Woodgett,J.R. and
            Penninger,J.M.
  TITLE     Stress-signalling kinase Sek1 protects thymocytes from apoptosis
            mediated by CD95 and CD3
  JOURNAL   Nature 385 (6614), 350-353 (1997)
   PUBMED   9002521
REFERENCE   7  (bases 1 to 3756)
  AUTHORS   Osugi,T., Ding,Y., Tanaka,H., Kuo,C.H., Do,E., Irie,Y. and Miki,N.
  TITLE     Involvement of a single-stranded DNA binding protein, ssCRE-BP/Pur
            alpha, in morphine dependence
  JOURNAL   FEBS Lett 391 (1-2), 11-16 (1996)
   PUBMED   8706895
REFERENCE   8  (bases 1 to 3756)
  AUTHORS   White,R.A., Hughes,R.T., Adkison,L.R., Bruns,G. and Zon,L.I.
  TITLE     The gene encoding protein kinase SEK1 maps to mouse chromosome 11
            and human chromosome 17
  JOURNAL   Genomics 34 (3), 430-432 (1996)
   PUBMED   8786147
REFERENCE   9  (bases 1 to 3756)
  AUTHORS   Salmeron,A., Ahmad,T.B., Carlile,G.W., Pappin,D., Narsimhan,R.P.
            and Ley,S.C.
  TITLE     Activation of MEK-1 and SEK-1 by Tpl-2 proto-oncoprotein, a novel
            MAP kinase kinase kinase
  JOURNAL   EMBO J 15 (4), 817-826 (1996)
   PUBMED   8631303
REFERENCE   10 (bases 1 to 3756)
  AUTHORS   Sanchez,I., Hughes,R.T., Mayer,B.J., Yee,K., Woodgett,J.R.,
            Avruch,J., Kyriakis,J.M. and Zon,L.I.
  TITLE     Role of SAPK/ERK kinase-1 in the stress-activated pathway
            regulating transcription factor c-Jun
  JOURNAL   Nature 372 (6508), 794-798 (1994)
   PUBMED   7997269
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY277234.1, AK036928.1 and AL662895.7.
            
            On Oct 20, 2015 this sequence version replaced NM_009157.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK036928.1, AK044698.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                BY277234.1         2-63
            63-2546             AK036928.1         5-2488
            2547-2547           AL662895.7         161743-161743       c
            2548-3076           AK036928.1         2490-3018
            3077-3655           AK036928.1         3020-3598
            3656-3656           AL662895.7         160634-160634       c
            3657-3756           AK036928.1         3600-3699
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 40.53 cM"
     gene            1..3756
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /note="mitogen-activated protein kinase kinase 4"
                     /db_xref="GeneID:26398"
                     /db_xref="MGI:MGI:1346869"
     exon            1..224
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1309
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /EC_number="2.7.12.2"
                     /note="isoform b is encoded by transcript variant 2; dual
                     specificity mitogen-activated protein kinase kinase 4; JNK
                     kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase
                     kinase 4; JNK-activating kinase 1; C-JUN N-terminal kinase
                     kinase 1; SAPK/Erk/kinase 1"
                     /codon_start=1
                     /product="dual specificity mitogen-activated protein
                     kinase kinase 4 isoform b"
                     /protein_id="NP_033183.1"
                     /db_xref="CCDS:CCDS24844.1"
                     /db_xref="GeneID:26398"
                     /db_xref="MGI:MGI:1346869"
                     /translation="MAAPSPSGGGGSGGGGGTPGPIGPPASGHPAVSSMQGKRKALKL
                     NFANPPVKSTARFTLNPNTTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKD
                     LGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIV
                     QFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLK
                     ENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPS
                     ASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREF
                     SPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERTVEVACYVCKILDQMPATPSS
                     PMYVD"
     exon            225..327
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            328..502
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            503..622
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            623..742
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            743..794
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            795..922
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1000
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1149
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1195
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..3756
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3629..3634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /note="hexamer: AATAAA"
     polyA_site      3649
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /note="major polyA site"
     regulatory      3711..3716
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /note="hexamer: ATTAAA"
     polyA_site      3732
                     /gene="Map2k4"
                     /gene_synonym="JNKK1; MAPKK 4; MEK4; MKK4; PRKMK4; Sek1;
                     Serk1"
                     /note="major polyA site"
ORIGIN      
        1 ggaggcgtgc ccggggcgtg gccaaggcgg cgcacgccgc gccctcagcg gagcgagagg
       61 ccgagcttgc tgcattgcag ccgccgcggc gccgctcggc tcttcacttc caacaatggc
      121 ggctccgagc ccgagcggcg gcggcggctc cgggggcggt ggcggcaccc cggggcccat
      181 cggacctcca gcttctggcc acccggcggt cagcagcatg cagggtaagc gcaaagcact
      241 gaagttgaat tttgcaaatc cacctgtcaa atcgacagca cggtttactc tgaatcctaa
      301 tactacagga gtccagaacc cacacataga gagactgaga acacacagca ttgagtcatc
      361 aggaaaactg aagatctccc ctgaacaaca ctgggatttc actgcagagg acttgaaaga
      421 ccttggagaa attggacgag gagcttatgg ttctgtcaac aaaatggtcc acaaaccaag
      481 tgggcagata atggcagtta aaagaattcg gtcaactgtg gatgaaaaag aacaaaaaca
      541 acttctcatg gatttggatg tagtaatgcg gagtagtgat tgcccataca ttgttcagtt
      601 ctatggtgca ctcttcagag agggcgactg ttggatctgt atggagctca tgtctacctc
      661 gttcgataag ttttacaaat atgtatatag tgtgttagat gacgttattc cggaagagat
      721 cttaggcaaa atcactttag caactgtgaa agcactaaac cacttaaaag aaaacttgaa
      781 aattattcac agagacatca aaccttccaa tattcttctg gacagaagtg gaaatataaa
      841 gctctgtgat ttcggcatca gtggacagct tgtggactct attgccaaga caagagatgc
      901 tgggtgtagg ccgtatatgg cacctgaaag aatagaccca agtgcatcaa gacaagggta
      961 tgatgtccgc tctgatgtct ggagtttggg gatcacattg tacgagttgg ccacaggccg
     1021 atttccttat ccaaagtgga atagtgtatt tgatcagcta acacaagtgg tgaaaggaga
     1081 ccctccgcag ctgagtaatt ctgaagaaag ggagttctcc cccagtttca tcaactttgt
     1141 caacttgtgc cttacgaagg atgaatccaa aaggccaaag tataaagagc ttctgaaaca
     1201 tccctttatt ttgatgtatg aagaacgtac tgtagaggtc gcatgctatg tttgtaaaat
     1261 cctggatcag atgccagcca ctcccagctc gcccatgtat gtcgactgat accgctgcta
     1321 catcagactc tagaaaaaag ggctgagggg aagcaagacg taaagagttt tcatcctgta
     1381 tcacagtgtt tttattgctc gcccagacac catgtgcaat aagactggtg ttcgtttcca
     1441 tcatgtctgt atacttctgt catctagact gtgcatccct gtaatacctg actgatcaca
     1501 gtgttagtgc tggtcagaga gacctcatcc tgctcttttg tgatgaacat attcatgacg
     1561 cgtggaagtc agtacgatca agttgttgac tgtgattaga tcacacctta attcatttct
     1621 agactcaaaa cctggagacg cagctactgg aatggtgttt tgtgagactt ccaaggctgg
     1681 aaagacacag tcatggatgt actgtctgag catagagaac ccgggcttga gtgagaagag
     1741 cttgcacagc cagtgagaca cattgccttc tgcagctggg agacagagga aggattccct
     1801 ttcttcacca agtgcaatag atttcctcat ctgataggct gttgctttac cgtcacacgc
     1861 gatgtgctca gccaaattcc ctactcaaaa tatcatgtta aattagaatt tatctttacc
     1921 aaaaaccatg ttgctttcag agaggtcaac attaaaatac tgagacagga cagaatgtgt
     1981 tctttgtcct tttccagccc tgctcttctc tgggaagata caggagtctg ccacctgtca
     2041 tagaagggtg ctgccaactt gatgctctac ctgccaccaa ccaccaggac tgaaggaagg
     2101 aaacagtact gagggcaaag tttacagatg tttttgattc tggcattttg tccagaacag
     2161 cttgtagcag ctatagattc ccccttggcc ccaagcctgg gacttgagcc atcgtaactc
     2221 actaacaggg agaattagct agtagcaatg tgccttgatg aagatttcct gcaggcagca
     2281 aaacaccaaa tcacaacgct ttgcttctcg acatcatggg ccaggtcctc agtttctaac
     2341 tctttccctt ccccacagtt cgttgtcgcc atgcggcttt tgctcagtct agtattctgc
     2401 cttgtttttc acattgggaa ccctaacatt accagggatg ctacttacca caatttgtaa
     2461 ccctgaatct ctcctgtgct ctggtgttca gaaagctgag acagctgggc ccagttgatg
     2521 ttgtacgtga gaaagaggca gtctgttgga gaggcatact tgagcaggat aaagatccta
     2581 aatggcagcc tttcagatag gcatgtctga cacctgctga ggcctctacc agttcactct
     2641 ctcccttgga agtggaattc tacccaccta aaccccccaa aaaagacaaa acgaaaagaa
     2701 gccacttcat cttatgtgcc gctgcttgtc ttcagcccat tagccaggta ctgagcgtca
     2761 ggctctgcat agcagttaag cagcactggt agtggtctgc cgtgcagggg atgcctgcta
     2821 tgcaggtgat gccctcccag gatctattcc tggtgggcac tgctgtgtct cccccagagt
     2881 ggcactcaaa gtactatcaa acaataaaag gaaaatggtg ctgtttgaag ttacatccct
     2941 tctctttgga ggatttaaaa atgatctatc ttttggtcca cttgcctaat tttcccattt
     3001 tccacccctg gccccctacc cttagattct ctgtgttcca caaggagaca tcatgagatg
     3061 tcttccttaa gctgcaccaa gccattgtcc tccgttgttc ccaggactca agaggaattt
     3121 gtttctctgc tgtcagcttc ctgtctggct cagtatagcc tcactctgcc gttatgcaaa
     3181 tggagatcaa agtaattctg actgtccagg agctatctca ctttctgctg tgtgggcggc
     3241 cacataaaaa ggcaatttta gtgtattaat catagattat tataaactat aaacttaagg
     3301 gcaaggaatt tattacaatg tatctttatt aaaacaaaag ggtgtatagt gttcacaaac
     3361 tgtgaaaata gtgtaagaac tgtacattgt gagctctggt tatttttctc ttgtaccata
     3421 tgaaagtata taaaaattat caaaaagctg atgtgcaggg atattgcctt atttgtctgt
     3481 aaaaatgtag ctcagtaaca taactgcttc ttggagcttt ggaatatttt atcctgtatt
     3541 cttgtttgaa tccttcctct atttaagaca tagccatgga gcccacatgt ttatggaata
     3601 gtctaccctt tgcctgcagg tcagttgtaa taaatctagg ttgtgatgat ggtcttgtaa
     3661 tctgattttc tgcagtcata ccccgagaaa gaaattcaag aaaaaatgac attaaatcat
     3721 ctgtgcattt catatacagg agagttatct ccttcc
//
