LOCUS       NM_008927               2396 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus mitogen-activated protein kinase kinase 1 (Map2k1),
            transcript variant 1, mRNA.
ACCESSION   NM_008927
VERSION     NM_008927.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Bjorklund,G.R., Rees,K.P., Balasubramanian,K., Hewitt,L.T.,
            Nishimura,K. and Newbern,J.M.
  TITLE     Hyperactivation of MEK1 in cortical glutamatergic neurons results
            in projection axon deficits and aberrant motor learning
  JOURNAL   Dis Model Mech 17 (6) (2024)
   PUBMED   38826084
  REMARK    GeneRIF: Hyperactivation of MEK1 in cortical glutamatergic neurons
            results in projection axon deficits and aberrant motor learning.
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Sun,M., Gao,Y., Li,Z., Yang,L., Liu,G., Xu,Z., Guo,R., You,Y. and
            Yang,Z.
  TITLE     ERK signaling expands mammalian cortical radial glial cells and
            extends the neurogenic period
  JOURNAL   Proc Natl Acad Sci U S A 121 (13), e2314802121 (2024)
   PUBMED   38498715
REFERENCE   3  (bases 1 to 2396)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2396)
  AUTHORS   Sudduth,C.L., Smits,P.J., Vivero,M.P., Cheng,Y.S., Ad,M.,
            Konczyk,D.J., Bischoff,J., Warman,M.L. and Greene,A.K.
  TITLE     Arteriovenous malformation Map2k1 mutation affects vasculogenesis
  JOURNAL   Sci Rep 13 (1), 11074 (2023)
   PUBMED   37422456
  REMARK    GeneRIF: Arteriovenous malformation Map2k1 mutation affects
            vasculogenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2396)
  AUTHORS   Kwon,H.N., Kurtzeborn,K., Iaroshenko,V., Jin,X., Loh,A.,
            Escande-Beillard,N., Reversade,B., Park,S. and Kuure,S.
  TITLE     Omics profiling identifies the regulatory functions of the MAPK/ERK
            pathway in nephron progenitor metabolism
  JOURNAL   Development 149 (19) (2022)
   PUBMED   36189831
REFERENCE   6  (bases 1 to 2396)
  AUTHORS   Han,J., Lee,J.D., Bibbs,L. and Ulevitch,R.J.
  TITLE     A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian
            cells
  JOURNAL   Science 265 (5173), 808-811 (1994)
   PUBMED   7914033
REFERENCE   7  (bases 1 to 2396)
  AUTHORS   Emslie,E.A., Jones,T.A., Sheer,D. and Keyse,S.M.
  TITLE     The CL100 gene, which encodes a dual specificity (Tyr/Thr) MAP
            kinase phosphatase, is highly conserved and maps to human
            chromosome 5q34
  JOURNAL   Hum Genet 93 (5), 513-516 (1994)
   PUBMED   8168826
REFERENCE   8  (bases 1 to 2396)
  AUTHORS   Brott,B.K., Alessandrini,A., Largaespada,D.A., Copeland,N.G.,
            Jenkins,N.A., Crews,C.M. and Erikson,R.L.
  TITLE     MEK2 is a kinase related to MEK1 and is differentially expressed in
            murine tissues
  JOURNAL   Cell Growth Differ 4 (11), 921-929 (1993)
   PUBMED   8297798
REFERENCE   9  (bases 1 to 2396)
  AUTHORS   Crews,C.M., Alessandrini,A. and Erikson,R.L.
  TITLE     The primary structure of MEK, a protein kinase that phosphorylates
            the ERK gene product
  JOURNAL   Science 258 (5081), 478-480 (1992)
   PUBMED   1411546
REFERENCE   10 (bases 1 to 2396)
  AUTHORS   Crews,C.M. and Erikson,R.L.
  TITLE     Purification of a murine protein-tyrosine/threonine kinase that
            phosphorylates and activates the Erk-1 gene product: relationship
            to the fission yeast byr1 gene product
  JOURNAL   Proc Natl Acad Sci U S A 89 (17), 8205-8209 (1992)
   PUBMED   1381507
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160118.2.
            
            On Aug 8, 2022 this sequence version replaced NM_008927.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK165894.1, BC054754.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-393               AC160118.2         155088-155480       c
            394-604             AC160118.2         116274-116484       c
            605-751             AC160118.2         114459-114605       c
            752-829             AC160118.2         106986-107063       c
            830-881             AC160118.2         106306-106357       c
            882-1006            AC160118.2         95577-95701         c
            1007-1208           AC160118.2         93328-93529         c
            1209-1273           AC160118.2         93142-93206         c
            1274-1335           AC160118.2         89552-89613         c
            1336-1381           AC160118.2         89166-89211         c
            1382-2396           AC160118.2         87659-88673         c
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 34.55 cM"
     gene            1..2396
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /note="mitogen-activated protein kinase kinase 1"
                     /db_xref="GeneID:26395"
                     /db_xref="MGI:MGI:1346866"
     exon            1..393
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             314..1495
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /EC_number="2.7.12.2"
                     /note="isoform 1 is encoded by transcript variant 1; dual
                     specificity mitogen-activated protein kinase kinase 1;
                     MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase,
                     mitogen activated, kinase 1, p45; MAP kinase kinase 1"
                     /codon_start=1
                     /product="dual specificity mitogen-activated protein
                     kinase kinase 1 isoform 1"
                     /protein_id="NP_032953.1"
                     /db_xref="CCDS:CCDS23277.1"
                     /db_xref="GeneID:26395"
                     /db_xref="MGI:MGI:1346866"
                     /translation="MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDE
                     QQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLE
                     IKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGR
                     IPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDS
                     MANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFG
                     CHVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEF
                     QDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAS
                     I"
     exon            394..604
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            605..751
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            752..829
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            830..881
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1006
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1208
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1273
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1335
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1381
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..2396
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2390..2395
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /note="hexamer: AATAAA"
     polyA_site      2396
                     /gene="Map2k1"
                     /gene_synonym="MAPKK1; Mek1; MEKK1; Prkmk1"
                     /note="major polyA site"
ORIGIN      
        1 agtccctcac tgggacgtct gtgcgcggcg tctcggagcg ccggagcagc ggtggccgca
       61 ctttctccaa gctggggctg tagctgagct gtgggtagtg cgcagggagc cgtccgagcc
      121 cgaggaaccg gtgtgctgag gcgagagttc ccggccggcg agcgcgcgca gctggttctc
      181 cgcgtgggtt gggcggaggg tcccaggagc gcggcgttga tcgagccgcc ccgactctgg
      241 gcagagccga gggaggaagc gagaagcggc cgcgcgctcc ctgctgagtt gcaggctctt
      301 tcccggctgc aagatgccca agaagaagcc gacgcccatc cagctgaacc cggcccccga
      361 tggctcggcg gttaacggga ccagctcggc cgagaccaac ctggaggcct tgcagaagaa
      421 gctggaggag ctggagcttg acgagcagca gcggaagcgg ctcgaggcct ttctgacgca
      481 gaagcagaag gtgggggaac tgaaggatga tgactttgag aagatcagcg aactgggagc
      541 tggcaacggt ggagtggtct tcaaggtctc ccacaagcca tctggcctgg ttatggctag
      601 aaagctgatc cacctggaga tcaaacccgc aatccggaac cagatcatcc gggagctgca
      661 ggtactgcac gagtgcaact ccccgtacat cgtgggcttc tacggggcct tctacagcga
      721 cggcgagatc agcatctgca tggagcacat ggatggtggg tccttggatc aagttctgaa
      781 gaaagctgga agaattcctg agcaaatttt aggaaaagtt agcattgctg tgataaaagg
      841 cctgacctat cttcgggaga agcacaagat tatgcacaga gatgtcaagc catccaacat
      901 tctagtgaac tcacgtgggg agatcaaact ctgtgatttt ggggtcagcg ggcagctaat
      961 tgactctatg gccaactcct tcgtgggcac gagatcctac atgtcgcctg agagactcca
     1021 ggggactcac tactctgtgc agtcggacat ctggagcatg gggctctctc tggtggagat
     1081 ggcagttggg agatacccca ttcctcctcc tgatgccaag gagctggagc tactgtttgg
     1141 atgccatgtg gaaggagacg cagccgaaac accacccagg ccaaggaccc ctgggaggcc
     1201 tctcagctca tatggaatgg acagccgacc tcccatggca atttttgagt tgttggatta
     1261 cattgtcaat gagcctcctc caaaactgcc cagtggagta ttcagtctgg agtttcagga
     1321 ttttgtgaat aaatgcttaa taaagaaccc tgcagagaga gcagatctga agcagctcat
     1381 ggtacatgct ttcatcaaaa gatctgacgc cgaggaggta gacttcgcag gctggctctg
     1441 ctccaccatt gggcttaacc agcccagcac accaacccac gctgccagca tctgagcctt
     1501 taggaagcag caaagaggaa ttctctgccc agtggcatgc catgttgctt tcaggcctct
     1561 cccatgcttg tctatgttca gacgtgcatc tcatctgtga caaaggatga agaacacagc
     1621 atgtgccaaa ttgtacttgt gtcattttta atatcattgt ctttatcact atggttactc
     1681 ccctaagtgg attggctttg tgcttggggc tatttgtctg ttcatcaaac acatgccagg
     1741 ctgaactaca gtgaaaccct agtgacctgg gtggtcgttc ttactgatgt ttgcactgct
     1801 gttcatcgtg actcactagc tggctgcctg tattgtcagg attctcggac cttggtactt
     1861 cactcttgct ggtgacctct cagtctgaga gggagccttg tgagaccctt cacaggcagt
     1921 gcatgcatgg aaagcatgct ttgctgctac tgaaatgagc atcagaacgt gtacgtcatg
     1981 gtatttttat tttttgcttt tggtatagaa ctcagcaatt cccatcaaaa aaacctaagc
     2041 agagcccatc actgccatga tagctgggct tcagtctgtc tactgtggtg atttttagac
     2101 ttctggttgt atttctatat ttatttttaa atatacagtg tgggatactt agtggtgtgt
     2161 gtctctaagt ttggattagt gtttctaaat tggtggttat tttgaatgtc acaaatggat
     2221 taaagcatca atgtatcaag agttctatct ttcttccagt ctaagtacca atgctattgt
     2281 aaacaacgtg tatagtgcct acaaattgta tgaaacccct tttaaccact ttaatcaaga
     2341 tgtttatcaa atctaatctc ttattctaat aaaaatacta tcaagttaaa ataaaa
//
