LOCUS       NM_008549               6091 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus mannosidase 2, alpha 1 (Man2a1), mRNA.
ACCESSION   NM_008549
VERSION     NM_008549.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6091)
  AUTHORS   Elkis,Y., Bel,S., Lerer-Goldstein,T., Nyska,A., Creasy,D.M.,
            Shpungin,S. and Nir,U.
  TITLE     Testosterone deficiency accompanied by testicular and epididymal
            abnormalities in TMF(-/-) mice
  JOURNAL   Mol Cell Endocrinol 365 (1), 52-63 (2013)
   PUBMED   23000399
REFERENCE   4  (bases 1 to 6091)
  AUTHORS   Tamaki,H., Sanda,M., Katsumata,O., Hara,Y., Fukaya,M. and
            Sakagami,H.
  TITLE     Pilt is a coiled-coil domain-containing protein that localizes at
            the trans-Golgi complex and regulates its structure
  JOURNAL   FEBS Lett 586 (19), 3064-3070 (2012)
   PUBMED   22841714
REFERENCE   5  (bases 1 to 6091)
  AUTHORS   Wang,W., Hale,C., Goulding,D., Haslam,S.M., Tissot,B., Lindsay,C.,
            Michell,S., Titball,R., Yu,J., Toribio,A.L., Rossi,R., Dell,A.,
            Bradley,A. and Dougan,G.
  TITLE     Mannosidase 2, alpha 1 deficiency is associated with ricin
            resistance in embryonic stem (ES) cells
  JOURNAL   PLoS One 6 (8), e22993 (2011)
   PUBMED   21886775
REFERENCE   6  (bases 1 to 6091)
  AUTHORS   Antony,C., Huchet,M., Changeux,J.P. and Cartaud,J.
  TITLE     Developmental regulation of membrane traffic organization during
            synaptogenesis in mouse diaphragm muscle
  JOURNAL   J Cell Biol 130 (4), 959-968 (1995)
   PUBMED   7642711
REFERENCE   7  (bases 1 to 6091)
  AUTHORS   Schneikert,J. and Herscovics,A.
  TITLE     Two naturally occurring mouse alpha-1,2-mannosidase IB cDNA clones
            differ in three point mutations. Mutation of Phe592 to Ser592 is
            sufficient to abolish enzyme activity
  JOURNAL   J Biol Chem 270 (30), 17736-17740 (1995)
   PUBMED   7629073
REFERENCE   8  (bases 1 to 6091)
  AUTHORS   Moremen,K.W. and Robbins,P.W.
  TITLE     Isolation, characterization, and expression of cDNAs encoding
            murine alpha-mannosidase II, a Golgi enzyme that controls
            conversion of high mannose to complex N-glycans
  JOURNAL   J Cell Biol 115 (6), 1521-1534 (1991)
   PUBMED   1757461
REFERENCE   9  (bases 1 to 6091)
  AUTHORS   Klein,J., Figueroa,F. and Klein,D.
  TITLE     H-2 haplotypes, genes, and antigens: second listing. I. Non-H-2
            loci on chromosome 17
  JOURNAL   Immunogenetics 16 (4), 285-317 (1982)
   PUBMED   6816725
  REMARK    Review article
REFERENCE   10 (bases 1 to 6091)
  AUTHORS   Dizik,M. and Elliott,R.W.
  TITLE     A gene apparently determining the extent of sialylation of
            lysosomal alpha-mannosidase in mouse liver
  JOURNAL   Biochem Genet 15 (1-2), 31-46 (1977)
   PUBMED   849251
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK144763.1, BC138372.1, BC138371.1, AK081775.1 and AI461855.1.
            
            On Apr 2, 2009 this sequence version replaced NM_008549.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X61172.1, BC138372.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AK144763.1         915-929
            16-1578             BC138372.1         1-1563
            1579-3913           BC138371.1         1564-3898
            3914-6074           AK081775.1         581-2741
            6075-6091           AI461855.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 33.76 cM"
     gene            1..6091
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /note="mannosidase 2, alpha 1"
                     /db_xref="GeneID:17158"
                     /db_xref="MGI:MGI:104669"
     exon            1..222
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..3540
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /EC_number="3.2.1.114"
                     /note="man II; AMan II; golgi alpha-mannosidase II;
                     mannosidase alpha class 2A member 1;
                     mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase"
                     /codon_start=1
                     /product="alpha-mannosidase 2"
                     /protein_id="NP_032575.2"
                     /db_xref="CCDS:CCDS28938.1"
                     /db_xref="GeneID:17158"
                     /db_xref="MGI:MGI:104669"
                     /translation="MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRGPRQEGSFP
                     QGQLSILQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGPRGSPGNASQGSIHLH
                     SPQLALQADPRDCLFASQSGSQPRDVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEW
                     DHEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSSRKFMWSEISYL
                     AKWWDIIDIPKKEAVKSLLQNGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLEKN
                     LGVKPRSGWAIDPFGHSPTMAYLLKRAGFSHMLIQRVHYAIKKHFSLHKTLEFFWRQN
                     WDLGSATDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISP
                     GNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDDFRFSEYTEWDLQCRNYEQLFSYMNS
                     QPHLKVKIQFGTLSDYFDALEKAVAAEKKSSQSVFPALSGDFFTYADRDDHYWSGYFT
                     SRPFYKRMDRIMESRIRAAEILYQLALKQAQKYKINKFLSSPHYTTLTEARRNLGLFQ
                     HHDAITGTAKDWVVVDYGTRLFQSLNSLEKIIGDSAFLLILKDKKLYQSDPSKAFLEM
                     DTKQSSQDSLPQKIIIQLSAQEPRYLVVYNPFEQERHSVVSIRVNSATVKVLSDSGKP
                     VEVQVSAVWNDMRTISQAAYEVSFLAHIPPLGLKVFKILESQSSSSHLADYVLYNNDG
                     LAENGIFHVKNMVDAGDAITIENPFLAIWFDRSGLMEKVRRKEDSRQHELKVQFLWYG
                     TTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRGRIYSDVTCFLEHVTHKVRLYNIQ
                     GIEGQSMEVSNIVNIRNVHNREIVMRISSKINNQNRYYTDLNGYQIQPRRTMSKLPLQ
                     ANVYPMCTMAYIQDAEHRLTLLSAQSLGASSMASGQIEVFMDRRLMQDDNRGLGQGVH
                     DNKITANLFRILLEKRSAVNMEEEKKSPVSYPSLLSHMTSSFLNHPFLPMVLSGQLPS
                     PAFELLSEFPLLQSSLPCDIHLVNLRTIQSKMGKGYSDEAALILHRKGFDCQFSSRGI
                     GLPCSTTQGKMSVLKLFNKFAVESLVPSSLSLMHSPPDAQNMSEVSLSPMEISTFRIR
                     LRWT"
     exon            223..474
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            475..619
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            620..791
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            792..919
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1093
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1280
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1458
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1661
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1844
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1959
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2030
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2196
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2418
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2541
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2656
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2657..2790
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2932
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2933..3066
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3067..3270
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3381
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3382..6078
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6055..6060
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /note="hexamer: AATAAA"
     polyA_site      6078
                     /gene="Man2a1"
                     /gene_synonym="Mana-2; Mana2; Map-2"
                     /note="major polyA site"
ORIGIN      
        1 agccctcggc gatcgccggc tggagtcctc agtcttctgc ctccttcggg tgaggaccgc
       61 gtcctggccc cgagtccatc gaggaaaatg aagttaagtc gccagttcac cgtgtttggc
      121 agcgcgatct tctgcgtcgt aatcttctca ctctacctga tgctggacag gggtcacttg
      181 gactaccctc ggggcccgcg ccaggagggc tcctttccgc agggccagct ttcaatattg
      241 caagaaaaga ttgaccattt ggagcgtttg ctcgctgaga acaacgagat catctcaaat
      301 atcagagact cagtcatcaa cctgagcgag tctgtggagg acggcccgcg ggggtcacca
      361 ggcaacgcca gccaaggctc catccacctc cactcgccac agttggccct gcaggctgac
      421 cccagagact gtttgtttgc ttcacagagt gggagtcagc cccgggatgt gcagatgttg
      481 gatgtttacg atctgattcc ttttgataat ccagatggtg gagtttggaa gcaaggattt
      541 gacattaagt atgaagcgga tgagtgggac catgagcccc tgcaagtgtt tgtggtgcct
      601 cactcccata atgacccagg ttggttgaag actttcaatg actactttag agacaagact
      661 cagtatattt ttaataacat ggtcctaaag ctgaaagaag actcaagcag gaagtttatg
      721 tggtctgaga tctcttacct tgcaaaatgg tgggatatta tagatattcc gaagaaggaa
      781 gctgttaaaa gtttactaca gaatggtcag ctggaaattg tgaccggtgg ctgggttatg
      841 cctgatgaag ccactccaca ttattttgcc ttaattgacc aactaattga agggcaccaa
      901 tggctggaaa aaaatctagg agtgaaacct cgatcgggct gggccataga tccctttggt
      961 cattcaccca caatggctta tcttctaaag cgtgctggat tttcacacat gctcatccag
     1021 agagtccatt atgcaatcaa aaaacacttc tctttgcata aaacgctgga gtttttctgg
     1081 agacagaatt gggatcttgg atctgctaca gacattttgt gccatatgat gcccttctac
     1141 agctacgaca tccctcacac ctgtgggcct gatcctaaaa tatgctgcca gtttgatttt
     1201 aaacggcttc ctggaggcag atatggttgt ccctggggag ttcccccaga agcaatatct
     1261 cctggaaatg tccaaagcag ggctcagatg ctattggatc agtaccggaa aaagtcaaaa
     1321 cttttccgca ctaaagttct gctggctcca ctgggagacg actttcggtt cagtgaatac
     1381 acagagtggg atctgcagtg caggaactac gagcaactgt tcagttacat gaactcgcag
     1441 cctcatctga aagtgaagat ccagtttgga accttgtcag attatttcga cgcattggag
     1501 aaagcggtgg cagccgagaa gaagagtagc cagtctgtgt tccctgccct gagtggagac
     1561 ttcttcacgt acgctgacag agacgaccat tactggagtg gctacttcac gtccagacct
     1621 ttctacaaac gaatggacag aataatggaa tctcgtataa gggctgctga aattctttac
     1681 cagttggcct tgaaacaagc tcagaaatac aagataaata aatttctttc atcacctcat
     1741 tacacaacac tgacagaagc cagaaggaac ttaggactat ttcagcatca tgatgccatc
     1801 acaggaaccg cgaaagactg ggtggttgtg gactatggta ccagactctt tcagtcatta
     1861 aattctttgg agaagataat tggagattct gcatttcttc tcattttaaa ggacaaaaag
     1921 ctgtaccagt cagatccttc caaagccttc ttagagatgg atacgaagca aagttcacaa
     1981 gattctctgc cccaaaaaat tataatacaa ctgagcgcac aggagccaag gtaccttgtg
     2041 gtctacaatc cctttgaaca agaacggcat tcagtggtgt ccatccgggt aaactccgcc
     2101 acagtgaaag tgctgtctga ttcgggaaaa ccggtggagg ttcaagtcag tgcagtttgg
     2161 aacgacatga ggacaatttc acaagcagcc tatgaggttt cttttctagc tcatatacca
     2221 ccactgggac tgaaagtgtt taagatctta gagtcacaaa gttcaagctc acacttggct
     2281 gattatgtcc tatataataa tgatggacta gcagaaaatg gaatattcca cgtgaagaac
     2341 atggtggatg ctggagatgc cataacaata gagaatccct tcctggcgat ttggtttgac
     2401 cgatctgggc tgatggagaa agtgagaagg aaagaagaca gtagacagca tgaactgaag
     2461 gtccagttcc tgtggtacgg aaccaccaac aaaagggaca agagcggtgc ctacctcttc
     2521 ctgcctgacg ggcagggcca gccatatgtt tccctaagac cgccctttgt cagagtgaca
     2581 cgtggaagga tctactcaga tgtgacctgt ttcctcgaac acgttactca caaagtccgc
     2641 ctgtacaaca ttcagggaat agaaggtcag tccatggaag tttctaatat tgtaaacatc
     2701 aggaatgtgc ataaccgtga gattgtaatg agaatttcat ctaaaataaa caaccaaaat
     2761 agatattata ctgacctaaa tggatatcag attcagccta gaaggaccat gagcaaattg
     2821 cctcttcaag ccaacgttta cccgatgtgc acaatggcgt atatccagga tgctgagcac
     2881 cggctcacgc tgctctctgc tcagtctcta ggtgcttcca gcatggcttc tggtcagatt
     2941 gaagtcttca tggatcgaag gctcatgcag gatgataacc gtggccttgg gcaaggcgtc
     3001 catgacaata agattacagc taatttgttt cgaatcctcc tcgagaagag aagcgctgtg
     3061 aacatggaag aagaaaagaa gagccctgtc agctaccctt ccctcctcag ccacatgact
     3121 tcgtccttcc tcaaccatcc ctttctcccc atggtactaa gtggccagct cccctcccct
     3181 gcctttgagc tgctgagtga atttcctctg ctgcagtcct ctctaccttg tgatatccat
     3241 ctggtcaacc tgcggacaat acaatcaaag atgggcaaag gctattcgga tgaggcagcc
     3301 ttgatcctcc acaggaaagg gtttgattgc cagttctcca gcagaggcat cgggctaccc
     3361 tgttccacta ctcagggaaa gatgtcagtt ctgaaacttt tcaacaagtt tgctgtggag
     3421 agtctcgtcc cttcctctct gtccttgatg cactcccctc cagatgccca gaacatgagt
     3481 gaagtcagcc tgagccccat ggagatcagc acgttccgta tccgcttgcg ttggacctga
     3541 cccagacttg agcagagaaa ctccggcttt gttaccgtcc ttggtttaac gtgcaataac
     3601 taagcacatt actttagctt ctggctactg tgagaacatg aattctgtga ttctgttttt
     3661 ttgttttgtt ttgttttgtt ttttaaacca atctagtaag aaaaagccat gctttaaaaa
     3721 aaggtttctt tctctgagtt tgtcccacag ctaccgttat cagtatgaac tgaggtaaca
     3781 aaaatgaaga actattgaaa gataagccct caacagactg catctcaggt taaatgctaa
     3841 ctatgtttgc attgagggtt gtaaagagat tctctagaag tattcttatc tctgatcatg
     3901 ttttattaaa aaaataccca tttgacttaa atagaaagaa aaatcagaag ttggtaggta
     3961 acagcttaat ggccaaagta gttgtaatca ttgatataaa tttgaatatt ttgtatgtta
     4021 aaataatgtg gaaaagtgca atccctcaac ccttatgata aatgtagttg gttttcatac
     4081 tcttttccaa tgaacctctt ggctaccctt tcctacccac acatttaaaa aaataagtga
     4141 taattttagt cgttcaccag tagaaaaaga ggtgtatttt cattacttga caacatggga
     4201 tgggtgcaat ttattccatt gtcactgaaa cagaagaagc agttccactt taggtaccac
     4261 aaggtgtctt tctatacagc tcatttgaat acaggtgttc caaggtgggg ttttctattt
     4321 ttaaattacc atatcaaaaa taaatgtgcc ttattttttt ataagtcttg ttaaatctta
     4381 ttactgtcca tattactgtt tgggaggtgg ggtattggga ggttgggata gggggtgggt
     4441 actttctatg acacataaat tatgtaaaat tttttgcttg ataatgctgg ccacatcctg
     4501 atctgtgtca ttacatttgt ggtgatgtta ctctaaacat tttgactatt tgaatgtact
     4561 gagatgtcag aaaacgaaac aagggagaaa aatattgcta attaaaatat gctgctgcca
     4621 aggaaactgc aagttgaagc aaggatttcg taacccacaa gatccaagta cttcttgcat
     4681 ttcacagtac ttaaccgcgt taaagagaga atgctttaaa aatagactgt tttctaaaat
     4741 cctctgtgtg tagctcatct tatgttcctt actaaagaac acgttccaaa catttgtgtg
     4801 tggtaaactc agcgtgttag tctagcccat gtaccatcat gggacctttg aggtcatttg
     4861 agatcattgt ttctgtaata gaataatagt atttaatttt ggtatagaaa gaatgctttg
     4921 gctatatcca cttttgaaaa attctcctca gtgtcttaac ctaatttagt attctttgac
     4981 tttaaacctc cacttacctc ttaccattcc agtacattac agagaaaagc atttctgcct
     5041 cactgtttat tctgatcgtc atctggattc ttcctcaaga tgcagctcct acattcgctg
     5101 ttctgttgag ttcctaaaat cctttgccta taatcattaa ggaaatacca gaatgacagg
     5161 accattgcaa gggtataatt tcatgcagta agcatgtgac gtacattccc ttcctcattt
     5221 gttggggaat aagcaactga aagtacaaca aaaccacttc ctcctataac ctctgcagac
     5281 gctagctagg acgaccaagc gccagtccca cgaatatgat ctattttctt gttgtatctt
     5341 aagtgtatgc cttcctgtgg atgttcactg tgctctgtgg ttaagtggct atgcttaatc
     5401 actacagaag ttctgatgag gccaaatgaa accagtggct gacataaata ataaacaaaa
     5461 acagcagaca caggggaccc actctgtgtt ctggccacca gctccttatt cacaggtgaa
     5521 gcagaaactc tatgtaacca gcaagcttct gctttttaaa gttattttca cattacaaca
     5581 ctggggaaat gaagagaggg tgtcttttgc tttgggttat ggtcagaaac tggtttctct
     5641 ggcacacgtg ggcagtcagc cacacttcag acctgacagc gggggagggg gggggcggtc
     5701 tgtttagtta ggcttggtac atctgttatc acagatgctc agtggaccaa gactgtctcg
     5761 ctactctctc cccactctct gaaattttaa ttttgtttag aggtgcatgg ttcaaatatc
     5821 agcattctaa aaaaaaaaat tgatttaaga aaatgctgta tgatgttaga agacattgta
     5881 attatcatcc cgtgtctttg ctgtactcgt gctgaagtgt tttcacagtc ctttgtctag
     5941 aagaaaatgt ttactttcat tgtgattatt ttgctcattg aaatcaacag gttttgatat
     6001 ttttctcttg gaagattttt ataacttttt gggaatatgt aatataagat ctctaataaa
     6061 atataatctt atcctgtgaa aaaaaaaaaa a
//
