LOCUS       NM_008548               5078 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus mannosidase 1, alpha (Man1a), mRNA.
ACCESSION   NM_008548
VERSION     NM_008548.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Zhang,L., Song,N.N., Zhang,Q., Mei,W.Y., He,C.H., Ma,P., Huang,Y.,
            Chen,J.Y., Mao,B., Lang,B. and Ding,Y.Q.
  TITLE     Satb2 is required for the regionalization of retrosplenial cortex
  JOURNAL   Cell Death Differ 27 (5), 1604-1617 (2020)
   PUBMED   31666685
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Xiang,Y., Karaveg,K. and Moremen,K.W.
  TITLE     Substrate recognition and catalysis by GH47 alpha-mannosidases
            involved in Asn-linked glycan maturation in the mammalian secretory
            pathway
  JOURNAL   Proc Natl Acad Sci U S A 113 (49), E7890-E7899 (2016)
   PUBMED   27856750
  REMARK    GeneRIF: in the bound substrate complex, hydrophobic stacking
            interactions between Trp residues and the glycan core anchor the
            base of the glycan structure to the enzyme cleft
REFERENCE   3  (bases 1 to 5078)
  AUTHORS   Gebuhr,I., Keeren,K., Vogt,K., Hoflich,C., Appelt,C., Schlieer,U.,
            Brandt,C., Meisel,C., Volk,H.D. and Sawitzki,B.
  TITLE     Differential expression and function of alpha-mannosidase I in
            stimulated naive and memory CD4+ T cells
  JOURNAL   J Immunother 34 (5), 428-437 (2011)
   PUBMED   21577142
  REMARK    GeneRIF: Complex N-glycans generated by enzymes such as
            a-mannosidase I inhibit the activation of naive T cells.
REFERENCE   4  (bases 1 to 5078)
  AUTHORS   Xie,Z., Ma,X., Ji,W., Zhou,G., Lu,Y., Xiang,Z., Wang,Y.X.,
            Zhang,L., Hu,Y., Ding,Y.Q. and Zhang,W.J.
  TITLE     Zbtb20 is essential for the specification of CA1 field identity in
            the developing hippocampus
  JOURNAL   Proc Natl Acad Sci U S A 107 (14), 6510-6515 (2010)
   PUBMED   20308569
REFERENCE   5  (bases 1 to 5078)
  AUTHORS   Sawitzki,B., Bushell,A., Steger,U., Jones,N., Risch,K., Siepert,A.,
            Lehmann,M., Schmitt-Knosalla,I., Vogt,K., Gebuhr,I., Wood,K. and
            Volk,H.D.
  TITLE     Identification of gene markers for the prediction of allograft
            rejection or permanent acceptance
  JOURNAL   Am J Transplant 7 (5), 1091-1102 (2007)
   PUBMED   17456197
  REMARK    GeneRIF: Downregulation of TOAG-1 and alpha-1,2-mannosidase
            expression occurred in graft infiltrating cells and expression of
            both genes was also downregulated after T-cell activation in vitro
REFERENCE   6  (bases 1 to 5078)
  AUTHORS   Beccari,T., Appolloni,M.G., Costanzi,E., Stinchi,S., Stirling,J.L.,
            Della Fazia,M.A., Servillo,G., Viola,M.P. and Orlacchio,A.
  TITLE     Lysosomal alpha-mannosidases of mouse tissues: characteristics of
            the isoenzymes, and cloning and expression of a full-length cDNA
  JOURNAL   Biochem J 327 (Pt 1) (Pt 1), 45-49 (1997)
   PUBMED   9355733
REFERENCE   7  (bases 1 to 5078)
  AUTHORS   Merkle,R.K., Zhang,Y., Ruest,P.J., Lal,A., Liao,Y.F. and
            Moremen,K.W.
  TITLE     Cloning, expression, purification, and characterization of the
            murine lysosomal acid alpha-mannosidase
  JOURNAL   Biochim Biophys Acta 1336 (2), 132-146 (1997)
   PUBMED   9305783
REFERENCE   8  (bases 1 to 5078)
  AUTHORS   Beccari,T., Appolloni,M.G., Stirling,J.L. and Orlacchio,A.
  TITLE     Assignment of lysosomal alpha-D-mannosidase to mouse chromosome 8
  JOURNAL   Mamm Genome 7 (9), 707-708 (1996)
   PUBMED   8829553
REFERENCE   9  (bases 1 to 5078)
  AUTHORS   Lal,A., Schutzbach,J.S., Forsee,W.T., Neame,P.J. and Moremen,K.W.
  TITLE     Isolation and expression of murine and rabbit cDNAs encoding an
            alpha 1,2-mannosidase involved in the processing of
            asparagine-linked oligosaccharides
  JOURNAL   J Biol Chem 269 (13), 9872-9881 (1994)
   PUBMED   8144580
REFERENCE   10 (bases 1 to 5078)
  AUTHORS   Herscovics,A., Schneikert,J., Athanassiadis,A. and Moremen,K.W.
  TITLE     Isolation of a mouse Golgi mannosidase cDNA, a member of a gene
            family conserved from yeast to mammals
  JOURNAL   J Biol Chem 269 (13), 9864-9871 (1994)
   PUBMED   8144579
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158642.8, AC164624.3 and AC153816.4.
            
            On Feb 7, 2019 this sequence version replaced NM_008548.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154820.1, AK043932.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-212               AC158642.8         138709-138920       c
            213-1067            AC158642.8         137599-138453       c
            1068-1164           AC164624.3         34199-34295
            1165-1280           AC164624.3         39164-39279
            1281-1361           AC164624.3         50498-50578
            1362-1456           AC164624.3         87876-87970
            1457-1580           AC153816.4         1807-1930
            1581-1674           AC153816.4         3234-3327
            1675-1790           AC153816.4         11274-11389
            1791-2010           AC153816.4         16557-16776
            2011-2183           AC153816.4         17489-17661
            2184-2299           AC153816.4         28761-28876
            2300-5078           AC153816.4         29256-32034
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 27.4 cM"
     gene            1..5078
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /note="mannosidase 1, alpha"
                     /db_xref="GeneID:17155"
                     /db_xref="MGI:MGI:104677"
     exon            1..212
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            213..1067
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     CDS             459..2426
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /EC_number="3.2.1.113"
                     /note="man9-mannosidase; alpha-1,2-mannosidase IA;
                     man(9)-alpha-mannosidase; mannosidase alpha class 1A
                     member 1; processing alpha-1,2-mannosidase IA;
                     mannosyl-oligosaccharide alpha-1,2-mannosidase"
                     /codon_start=1
                     /product="mannosyl-oligosaccharide 1,2-alpha-mannosidase
                     IA"
                     /protein_id="NP_032574.1"
                     /db_xref="CCDS:CCDS23848.1"
                     /db_xref="GeneID:17155"
                     /db_xref="MGI:MGI:104677"
                     /translation="MPVGGLLPLFSSPGGGGLGSGLGGGLGGGRKGSGPAAFRLTEKF
                     VLLLVFSAFITLCFGAIFFLPDSSKLLSGVLFHSNPALQPPAEHKPGLGARAEDAAEG
                     RVRHREEGAPGDPGAGLEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKEKVA
                     QDQLRDKDLFRGLPKVDFLPPVGVENREPADATIREKRAKIKEMMTHAWNNYKRYAWG
                     LNELKPISKEGHSSSLFGNIKGATIVDALDTLFIMGMKTEFQEAKSWIKKYLDFNVNA
                     EVSVFEVNIRFVGGLLSAYYLSGEEIFRKKAVELGVKLLPAFHTPSGIPWALLNMKSG
                     IGRNWPWASGGSSILAEFGTLHLEFMHLSHLSGDPVFAEKVMKIRTVLNKLDKPEGLY
                     PNYLNPSSGQWGQHHVSVGGLGDSFYEYLLKAWLMSDKTDLEAKKMYFDAVQAIETHL
                     IRKSSGGLTYIAEWKGGLLEHKMGHLTCFAGGMFALGADGAPEARAQHYLELGAEIAR
                     TCHESYNRTYVKLGPEAFRFDGGVEAIATRQNEKYYILRPEVIETYMYMWRLTHDPKY
                     RTWAWEAVEALESHCRVNGGYSGLRDVYIARESYDDVQQSFFLAETLKYLYLIFSDDD
                     LLPLEHWIFNTEAHPFPILREQKKEIDGKEK"
     exon            1068..1164
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1280
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1361
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1456
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1580
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1674
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1790
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..2010
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..2183
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2299
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..5078
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5055..5060
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /note="hexamer: AATAAA"
     polyA_site      5078
                     /gene="Man1a"
                     /gene_synonym="Man1a1; PCR1"
                     /note="major polyA site"
ORIGIN      
        1 agtgcacggc ggggccgcga cggagggcag tacggtgcct ggttcgctgg ctcacgccgg
       61 caggtcggtg cgctcgctca cctgagccgt gtcccggggc ctcagagcca cccggctgta
      121 cgcgtgctac cacccgccgg gaagcgctcg gagatgggca gctggagaag ttgacgaacc
      181 ccctacgcac gcggcgccct tgctgcccag aggggctgca gccagcggtt tgccgcgcgt
      241 gcctgtttgc caaaggagcc gccccggcga ggatcgccgg aggggagccg cttcaagcgg
      301 ggagacccgc agcccagaca cgcgctctgg gcggcctgga tgcgcaagag ccggccccgc
      361 agccgctgac agccggcggc agcgaagttt ggctgctgag cggctcggcg ccgggcccct
      421 ggacagactg tcagccggcg gctacaacct ccggagcgat gcccgtgggg ggcctgttgc
      481 cgctcttcag tagccctggg ggcggcggcc tgggcagtgg cctgggcggg gggcttggcg
      541 gcgggaggaa ggggtctggc cccgctgcct tccgcctcac cgagaagttc gtgctgctgc
      601 tggtgttcag cgccttcatc acgctctgct tcggggcaat cttcttcctg cctgactcct
      661 ccaagctgct cagcggggtc ctgttccact ccaaccctgc cttgcagccg ccggcggagc
      721 acaagcccgg gctcggggcg cgtgcggagg atgccgccga ggggagagtc cggcaccgcg
      781 aggaaggcgc gcctggggac cctggagctg gactggaaga caacttagcc aggatccgcg
      841 aaaaccacga gcgggctctc agggaagcca aggagaccct gcagaagctg ccggaggaga
      901 tccaaagaga cattctgctg gagaaggaaa aggtggccca ggaccagctg cgtgacaagg
      961 atctgtttag gggcttgccc aaggtggact tcctgccccc cgtcggggta gagaaccggg
     1021 agcccgctga cgccaccatc cgtgagaaga gggcaaagat caaagagatg atgacccatg
     1081 cttggaataa ttataaacgc tatgcgtggg gcttgaacga actgaaacct atatcaaaag
     1141 aaggccattc aagcagtttg tttggcaaca tcaaaggagc tacaatagta gatgccctgg
     1201 ataccctttt cattatgggc atgaagactg aatttcaaga agctaaatcg tggattaaaa
     1261 aatatttaga ttttaatgtg aatgctgaag tttctgtttt tgaagtcaac atacgcttcg
     1321 tcggtggact gctgtcagcc tactatttgt ccggagagga gatatttcga aagaaagcag
     1381 tggaacttgg ggtaaaattg ctacctgcat ttcatactcc ctctggaata ccttgggcat
     1441 tgctgaatat gaaaagtggg atcgggcgga actggccctg ggcctctgga ggcagcagta
     1501 tcctggccga atttggaact ctgcatttag agtttatgca cttgtcccac ttatcaggag
     1561 acccagtctt tgccgaaaag gttatgaaaa ttcgaacagt gttgaacaaa ctggacaaac
     1621 cagaaggcct ttatcctaac tatctgaacc ccagtagtgg acagtggggt caacatcatg
     1681 tgtcggttgg aggacttgga gacagctttt atgaatattt gcttaaggcg tggttaatgt
     1741 ctgacaagac agatctcgaa gccaagaaga tgtattttga tgctgttcag gccatcgaga
     1801 ctcacttgat ccgcaagtca agtgggggac taacgtacat cgcagagtgg aaggggggcc
     1861 tcctggaaca caagatgggc cacctgacgt gctttgcagg aggcatgttt gcacttgggg
     1921 cagatggagc tccggaagcc cgggcccaac actaccttga actcggagct gaaattgccc
     1981 gcacttgtca tgaatcttat aatcgtacat atgtgaagtt gggaccggaa gcgtttcgat
     2041 ttgatggcgg tgtggaagct attgccacga ggcaaaatga aaagtattac atcttacggc
     2101 ccgaggtcat cgagacatac atgtacatgt ggcgactgac tcacgacccc aagtacagga
     2161 cctgggcctg ggaagccgtg gaggctctag aaagtcactg cagagtgaac ggaggctact
     2221 caggcttacg ggatgtttac attgcccgtg agagttatga cgatgtccag caaagtttct
     2281 tcctggcaga gacactgaag tatttgtact tgatattttc cgatgatgac cttcttccac
     2341 tagaacactg gatcttcaac accgaggctc atcctttccc tatactccgt gaacagaaga
     2401 aggaaattga tggcaaagag aaatgaaaga cattttctat tttactctgc ttcgttccct
     2461 cactgcatac cttaataatt ccttttctgg taatcagaca tatgatgaac tcttattgat
     2521 tggcaaacgt ctatgattct cctaagttct acaattgttt tgcagtcttt tatgtttagt
     2581 gccataagcc taggtggacc tcaattcctt attcagctag tagatccaca gattctgcct
     2641 gtctttttct ttttctcaag gaatctattg tttctgaaat tcataaagtt gcaaaatcat
     2701 tttcataata gaaccgtcta ccagtgacct cttgattatg atttgatttg actgcaaaac
     2761 ttcttcctcc tctatgagga gatgctgtct gcttcgagct gtaatgtttt gccatgttgc
     2821 aaaaagccat aataataaat taagtattta aaaaaaaaaa gctttttttt ccttttcaag
     2881 ttcatgttaa tctggtttgt ctgtccacca gaaacaggtc ttacaactgt gacagcctct
     2941 tagcaggtct atcgttattt gatagaatgt cttctagagt actccactct cattgtgatt
     3001 cagatgagaa agccatccca gaagagctgg cgacactggc ctcatctctt cacaagtgca
     3061 gtatcttctt attgggtgat tatgttcatg tttcctcact ttcatttcgt tctctgcttt
     3121 tgctctaaat gccctgtgtc atctctgtat cacatactgc ttaatttctc ccatcaccca
     3181 cgacacagca aggggagctc ctgagttatg cagaatgcca ggttacccaa ttacaatttt
     3241 gaaagagaaa agacctaact tctaacatgc caggttttcc tttttaatga atccattcca
     3301 aaatctcctc tctaaaaaga gagggagaat tccatatgac tagctcatgg gtatacattt
     3361 cctctttggg tctctttaag caaaggatgc attgcacttg gttcagatct gaaaaggtct
     3421 cttcccaggt gtagctcatc attgaacttc agtttatgaa catgagcttt tacccagtga
     3481 aaaaagcatt tctgatttac ctactcctat tcagctaaag gagaaagtaa aatctaatta
     3541 actcttaagg acacactgta ttaaatgtgc ttacattcca cataaagatc ttaacttaca
     3601 aataatttat agggatggat ccactccctc ataatttgat ctgcaacaga tcaaaatgat
     3661 tatatgattc ttctaaacct tatcttgaac tatgaatgat tttcccaagg acaaattttg
     3721 tgatagcttg tgttaccaca cctgttcttt atatgaaaga tcagatatga ttcatatgta
     3781 ctgaaagaca aagacaagaa ccacctcaat ttcaacctac tatggtcaat aaacaacaaa
     3841 aacaaataat acagatccat ggattaggct aaagtatata tttctgtgca cttacttttt
     3901 tcaaaatgtc taattttatc aatttcttat ggtacaacct attttttgag acatttgttt
     3961 tcttaaatct gagtagacaa tagtttcacc tatttcagct ttggggatgg tttcaaaaga
     4021 tgttattttg aatcactacc ttgtatgccc tggttgtctc cagtataata cactagaaca
     4081 catcaaaaca aaacagtaaa attagtaaat ggaaaagatc cctcagtata tcattttgac
     4141 tatattaaca catctctgct acccagagaa gcttctgcct gtaatctatt cagtctctgg
     4201 aggttatgaa cccatgacat cattttgatg aaactgaata gaacagtctg ccttacctct
     4261 tcattacaac ttgatttgac tgtaaaactt ttccctccgt gaggaagaac acgtgaacaa
     4321 cagatccttc cttccctaga gctcagaggt aaaatgttaa aatttgctgc caaaatttgg
     4381 tcacctgtat ttttattata attttcactg cccttagatt agctatttaa ttcattttct
     4441 ttgcatttca atttgaagga gcatccacag ctggtgatta tttctgcagc aggaggggtt
     4501 tcaaaggcat gcgctgtgta cctgctacac tgtaatgcag cttttatgtt ccactataag
     4561 cagggaagaa gctgaaccac aggttgtggt cccactggca agttttcagt tttaaaacca
     4621 gatctcagaa caccaaaaac ttaaacgaga taagagatta attttataga aatgtaactg
     4681 attaagctta tgttttgtgt taattttaaa caacggaagt ctccaaatac ataccaacgc
     4741 attcattttc aacaaagttg atttacatgt ataatccaca cccttaaaga tgaacagtta
     4801 gagtagcacc cagttttgtg tttacaaaac ctgttttgat atactatatt atacatagaa
     4861 gttgtatgcc aatttataga actcagtgac ttctctatta tgtgtatcat ccatttttta
     4921 cattgattgt ttttgatttg ttcataagag cattctgtca tttccaactt tatataaatc
     4981 tctgatgtta tggaaaacat aatagattga cacataattt ttaaaaatta tatttgtaaa
     5041 atttctctat tgtgaataaa gtcttttaat atacctcc
//
