LOCUS       NM_021500               2128 bp    mRNA    linear   ROD 02-FEB-2024
DEFINITION  Mus musculus macrophage erythroblast attacher (Maea), mRNA.
ACCESSION   NM_021500
VERSION     NM_021500.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Wei,Q., Pinho,S., Dong,S., Pierce,H., Li,H., Nakahara,F., Xu,J.,
            Xu,C., Boulais,P.E., Zhang,D., Maryanovich,M., Cuervo,A.M. and
            Frenette,P.S.
  TITLE     MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance
            of haematopoietic stem cells
  JOURNAL   Nat Commun 12 (1), 2522 (2021)
   PUBMED   33947846
  REMARK    GeneRIF: MAEA is an E3 ubiquitin ligase promoting autophagy and
            maintenance of haematopoietic stem cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Fan,H., Li,N., Fan,P., Hu,X., Liang,K., Zhang,S., Cheng,X. and
            Wu,Y.
  TITLE     Differential tissue expression of erythroblast macrophage protein
            in a MRL/lpr mouse model of lupus
  JOURNAL   Lupus 28 (7), 843-853 (2019)
   PUBMED   31132907
  REMARK    GeneRIF: The data showed that erythroblast macrophage protein (EMP)
            is ubiquitously expressed in macrophages at all of the tissues of
            the lupus and control mice. However, the expression of EMP in bone
            marrow and liver tissues of lupus mice was higher than in the
            control mice, which indicates that EMP may be important in the
            development of systemic lupus erythematosus.
REFERENCE   3  (bases 1 to 2128)
  AUTHORS   Wei,Q., Boulais,P.E., Zhang,D., Pinho,S., Tanaka,M. and
            Frenette,P.S.
  TITLE     Maea expressed by macrophages, but not erythroblasts, maintains
            postnatal murine bone marrow erythroblastic islands
  JOURNAL   Blood 133 (11), 1222-1232 (2019)
   PUBMED   30674470
REFERENCE   4  (bases 1 to 2128)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. PMID: 29144450]
REFERENCE   5  (bases 1 to 2128)
  AUTHORS   Javan,G.T., Can,I., Yeboah,F., Lee,Y. and Soni,S.
  TITLE     Novel interactions between erythroblast macrophage protein and cell
            migration
  JOURNAL   Blood Cells Mol Dis 60, 24-27 (2016)
   PUBMED   27519940
  REMARK    GeneRIF: results implicate Emp in abnormal cell motility in
            macrophage cells
REFERENCE   6  (bases 1 to 2128)
  AUTHORS   Zou,Z., Enis,D.R., Bui,H., Khandros,E., Kumar,V., Jakus,Z.,
            Thom,C., Yang,Y., Dhillon,V., Chen,M., Lu,M., Weiss,M.J. and
            Kahn,M.L.
  TITLE     The secreted lymphangiogenic factor CCBE1 is essential for fetal
            liver erythropoiesis
  JOURNAL   Blood 121 (16), 3228-3236 (2013)
   PUBMED   23426945
REFERENCE   7  (bases 1 to 2128)
  AUTHORS   Soni,S., Bala,S. and Hanspal,M.
  TITLE     Requirement for erythroblast-macrophage protein (Emp) in definitive
            erythropoiesis
  JOURNAL   Blood Cells Mol Dis 41 (2), 141-147 (2008)
   PUBMED   18501646
  REMARK    GeneRIF: Emp plays a cell intrinsic role in the erythroid lineage
REFERENCE   8  (bases 1 to 2128)
  AUTHORS   Soni,S., Bala,S., Kumar,A. and Hanspal,M.
  TITLE     Changing pattern of the subcellular distribution of erythroblast
            macrophage protein (Emp) during macrophage differentiation
  JOURNAL   Blood Cells Mol Dis 38 (1), 25-31 (2007)
   PUBMED   17071116
  REMARK    GeneRIF: Temporal and spatial expression of Emp correlates with its
            role in erythroblastic island formation, suggesting that Emp may be
            involved in multiple cellular functions.
REFERENCE   9  (bases 1 to 2128)
  AUTHORS   Soni,S., Bala,S., Gwynn,B., Sahr,K.E., Peters,L.L. and Hanspal,M.
  TITLE     Absence of erythroblast macrophage protein (Emp) leads to failure
            of erythroblast nuclear extrusion
  JOURNAL   J Biol Chem 281 (29), 20181-20189 (2006)
   PUBMED   16707498
  REMARK    GeneRIF: Emp null model provides an experimental system to study
            enucleation process and to evaluate the function of macrophages in
            definitive erythropoiesis.
REFERENCE   10 (bases 1 to 2128)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CX212584.1 and BC058687.1.
            
            [WARNING] On Apr 6, 2024 this sequence was replaced by NM_021500.3.
            
            On Dec 29, 2006 this sequence version replaced NM_021500.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058687.1, AK145800.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 CX212584.1         1-2
            3-2128              BC058687.1         1-2126
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 17.55 cM"
     gene            1..2128
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /note="macrophage erythroblast attacher"
                     /db_xref="GeneID:59003"
                     /db_xref="MGI:MGI:1891748"
     exon            1..74
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
     CDS             6..1196
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /EC_number="2.3.2.27"
                     /note="erythroblast macrophage protein"
                     /codon_start=1
                     /product="E3 ubiquitin-protein transferase MAEA"
                     /protein_id="NP_067475.2"
                     /db_xref="CCDS:CCDS39063.1"
                     /db_xref="GeneID:59003"
                     /db_xref="MGI:MGI:1891748"
                     /translation="MAVQESAAQLSMTLKVQEYPTLKVPYETLNKRFRAAQKNIDRET
                     SHVTMVVAELEKTLSSCPAVDSVVSLLDGVVEKLSVLKRKAVESIQAEDESAKLCKRR
                     IEHLKEHSSDQPAAASMWKRKRMDRMMVEHLLRCGYYNTAVKLARQSGIEDLVNIEMF
                     LTAKEVEESLERRETATCLAWCHDNKSRLRKMKSCLEFSLRIQEFIELVRQNKRLDAV
                     RHARKHFSQAEGSQLDEVRQVMGMLAFPPDTHISPYKDLLDPARWRMLIQQFRYDNYR
                     LHQLGNSSVFTLTLQAGLSAIKTPQCYKEDGSSKSPDCPVCSRSLNKLAQPLPMAHCA
                     NSRLVCKISGDVMNENNPPMMLPNGYVYGYNSLLSIRQDDKVVCPRTKEVFHFSQAEK
                     VYIM"
     exon            75..257
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
     exon            258..461
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
     exon            462..584
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
     exon            585..661
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
     exon            662..770
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
     exon            771..904
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1100
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..2128
                     /gene="Maea"
                     /gene_synonym="1110030D19Rik; EMP; Gid9"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccaagatggc ggtgcaggag tcggcggccc agctgtccat gaccctgaag gtgcaggagt
       61 acccgaccct caaggtgccc tatgagacac tgaacaaacg cttccgagct gctcagaaaa
      121 acatcgatcg agagactagc cacgtcacca tggtggtagc tgagcttgag aagaccttga
      181 gtagttgccc agctgtggac tctgtggtca gcctattgga tggtgtggtg gagaagctga
      241 gtgtcctcaa gaggaaggca gtagagtcca tccaggccga ggatgagagc gccaagctct
      301 gcaaacgtag gatcgagcac ctcaaggagc acagcagtga ccagccagca gcagccagca
      361 tgtggaagcg gaagcgcatg gaccggatga tggtggagca tctgctacgc tgtggctact
      421 acaacacagc tgtgaagctg gctcgccaga gtggcatcga ggaccttgtg aatatcgaga
      481 tgttcctgac agccaaagaa gtggaggagt ccttggagag gcgtgagaca gccacctgcc
      541 ttgcctggtg ccatgataac aagtcccgac tccggaagat gaagagctgc ctagagttca
      601 gcctcaggat tcaggagttc attgaacttg tccggcagaa caagcgcctg gatgctgtga
      661 gacatgcaag aaagcacttc agtcaggctg aagggagcca gctggatgag gtccgccagg
      721 tcatgggcat gttggccttc ccaccagaca cacatatctc cccatacaag gacctcctgg
      781 acccagcccg gtggcgaatg ctgatccagc agtttcgata tgataactac cggctgcacc
      841 agctgggaaa cagctcagtc ttcaccctca ccctgcaggc tgggctctca gcaataaaga
      901 caccacagtg ctacaaggag gatggcagct ctaagagccc tgactgccct gtgtgcagcc
      961 gctctctgaa caaactggca cagcccctgc ccatggctca ctgtgccaac tcccgcctgg
     1021 tctgcaagat ctctggtgac gtgatgaatg agaataaccc acccatgatg ctgcctaatg
     1081 gctatgtcta tggctacaat tctctgcttt ctattcgtca agatgataaa gttgtttgcc
     1141 caagaaccaa agaagtcttc cacttctccc aagctgagaa agtatacatc atgtaggccc
     1201 cgagttgcta tgggcacgcc cttggccaga ggcggtggtg ggtgggagcc atgccctcct
     1261 gccctgaccc ctgaccccag accccagcct gcatcgacat ttgtttcttg cgaccaaaga
     1321 tcagtgagca acgacaaata ctcttagaaa aagaggaagt gagatttaat aagtctgtgc
     1381 ttgagaacag cgtttgattg gtaggatttg taacataagt cgacgatgcc tctgcagggt
     1441 cttgcacctc tcaaaggaaa ctttctctac agtgtgacct ggaggttcac ccaggaaatc
     1501 cagagcatct cagaagtagg aattggttca tcggttattt ccctgtgttt ctccgtttcc
     1561 ttccttttgc tagtgatggg actcagggga gcttgaggag caggctggcc ctttgccaac
     1621 tcctgttggt cacagcttgg gcaaggtgct ggccacacca ggttggctgc agtgacttgt
     1681 gacgtggtgc cgaggccttt cctgacttca ctgtctagct gctgccaaag cgccgtgcac
     1741 cacatgcacc tgggctgcta cttgaggagg ctctgcccac gtggccagtg tctggggctg
     1801 tgtctgcaca ggttcagcat ctcaccttcc agcaccgcct tatctcctgc tttgagaatg
     1861 tattcacggg aaagccactg gactgagttt ctgcagaggc cttgccaggt ggttccgtag
     1921 tcctagagtg tacatgcttt tcattacaga aactgatagt tcagctaaaa taagtactga
     1981 cgggttttca aaacaaatga accattgtaa ttcacacaca cccatattgt agaggtttgt
     2041 atctagcgct tatttttgca tgttgatgtt gattagctaa tgaactgtaa atgtaaagtg
     2101 tgttaataaa atagctttct atttctca
//
