LOCUS       NM_001382835             994 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus lysozyme-like 4 (Lyzl4), transcript variant 3, mRNA.
ACCESSION   NM_001382835
VERSION     NM_001382835.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   2  (bases 1 to 994)
  AUTHORS   Sun,R., Shen,R., Li,J., Xu,G., Chi,J., Li,L., Ren,J., Wang,Z. and
            Fei,J.
  TITLE     Lyzl4, a novel mouse sperm-related protein, is involved in
            fertilization
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 43 (5), 346-353 (2011)
   PUBMED   21444326
  REMARK    GeneRIF: Immuno-neutralization of Lyzl4 protein in spermatozoa with
            its specific antibody significantly decreased in vitro
            fertilization percentage in a dose-dependent manner, suggesting
            that Lyzl4 might be important for fertilization
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131660.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CA465592.1, SRR5189685.72721.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression,
                                                longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-269               AC131660.4         10486-10754
            270-519             AC131660.4         12864-13113
            520-669             AC131660.4         13226-13375
            670-748             AC131660.4         14388-14466
            749-994             AC131660.4         19353-19598
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 72.45 cM"
     gene            1..994
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /note="lysozyme-like 4"
                     /db_xref="GeneID:69032"
                     /db_xref="MGI:MGI:1916282"
     exon            1..269
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /inference="alignment:Splign:2.1.0"
     exon            270..519
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /inference="alignment:Splign:2.1.0"
     CDS             381..818
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /note="lysozyme-like protein 4; lysozyme a"
                     /codon_start=1
                     /product="lysozyme-like protein 4 precursor"
                     /protein_id="NP_001369764.1"
                     /db_xref="GeneID:69032"
                     /db_xref="MGI:MGI:1916282"
                     /translation="MQLYLVLLLISYLLTPIGASILGRCTVAKMLYDGGLNYFEGYSL
                     ENWVCLAYFESKFNPSAVYEDPQDGSTGFGLFQIRDNEWCGHGKNLCSVSCTALLNPN
                     LKDTIQCAKKIVKGKHGMGAWPIWSKNCQLSDVLDRWLDGCDL"
     exon            520..669
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /inference="alignment:Splign:2.1.0"
     exon            670..748
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /inference="alignment:Splign:2.1.0"
     exon            749..994
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /inference="alignment:Splign:2.1.0"
     regulatory      974..979
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /note="hexamer: AATAAA"
     polyA_site      994
                     /gene="Lyzl4"
                     /gene_synonym="1810009N24Rik; Lyc4; Lyza"
                     /note="major polyA site"
ORIGIN      
        1 aggttctaga agagtttctc atggagcagg atcgatggtg tagaaactct tgggtgaaga
       61 caaatgggtt attaagaaag tggcatctga gaaagtgcag gggacccagt gccggcagag
      121 tacccagcct gcaagcaacg tggactccct gccgggctgg taggtgggcc tttgtgggca
      181 gaagcagaga caggcaaacg acatctggct atagagcaca tttgtcagca aagtcccctt
      241 ttggtcatct ggagacacat tttggaaggt gcctctcctt tccctggaaa gtccagtgtc
      301 cctatgacct tggaaggagc tgcctgagac tctctgttca gccggcatcc acagcttcga
      361 gccaccagag cttggcgacg atgcagctgt acctggtgct tctcctcatc agctacttgc
      421 tgactccgat cggtgcttcc atcttggggc gctgcacagt ggctaagatg ctctatgatg
      481 ggggcttgaa ttattttgag ggctacagcc ttgaaaactg ggtgtgcctg gcttattttg
      541 agagcaagtt caacccctca gctgtctatg aggacccaca agatggctcc accggtttcg
      601 gcctctttca gattcgtgac aatgaatggt gcggccacgg caagaacctc tgcagtgtgt
      661 cctgcactgc tttactgaat ccaaacttaa aggatacgat ccagtgtgcc aagaaaatcg
      721 tgaaaggaaa acatgggatg ggagcgtggc ccatctggtc caagaactgc cagctgtctg
      781 acgttctgga ccggtggctg gacggctgtg atctgtaggc acccgagtgg ccctgcctca
      841 cttgccagcc tcgtcttggg actgaggatg ctctcctgtt tgctgcttca atccacccag
      901 ttacctcacc actggccagc tccaggtgga aaaggacggc agtcctcact tcatccaggg
      961 gtgcaaggtg cagaataaac cagctaatta ctca
//
