LOCUS       NM_177140               4141 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus lysosomal enzyme trafficking factor (Lyset),
            transcript variant 1, mRNA.
ACCESSION   NM_177140
VERSION     NM_177140.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Richards,C.M., Jabs,S., Qiao,W., Varanese,L.D., Schweizer,M.,
            Mosen,P.R., Riley,N.M., Klussendorf,M., Zengel,J.R., Flynn,R.A.,
            Rustagi,A., Widen,J.C., Peters,C.E., Ooi,Y.S., Xie,X., Shi,P.Y.,
            Bartenschlager,R., Puschnik,A.S., Bogyo,M., Bertozzi,C.R.,
            Blish,C.A., Winter,D., Nagamine,C.M., Braulke,T. and Carette,J.E.
  TITLE     The human disease gene LYSET is essential for lysosomal enzyme
            transport and viral infection
  JOURNAL   Science 378 (6615), eabn5648 (2022)
   PUBMED   36074821
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Zhang,W., Yang,X., Li,Y., Yu,L., Zhang,B., Zhang,J., Cho,W.J.,
            Venkatarangan,V., Chen,L., Burugula,B.B., Bui,S., Wang,Y., Duan,C.,
            Kitzman,J.O. and Li,M.
  TITLE     GCAF(TMEM251) regulates lysosome biogenesis by activating the
            mannose-6-phosphate pathway
  JOURNAL   Nat Commun 13 (1), 5351 (2022)
   PUBMED   36096887
  REMARK    GeneRIF: GCAF(TMEM251) regulates lysosome biogenesis by activating
            the mannose-6-phosphate pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4141)
  AUTHORS   Nelson,P.S., Pritchard,C., Abbott,D. and Clegg,N.
  TITLE     The human (PEDB) and mouse (mPEDB) Prostate Expression Databases
  JOURNAL   Nucleic Acids Res 30 (1), 218-220 (2002)
   PUBMED   11752298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132315.4.
            
            On Jan 25, 2023 this sequence version replaced NM_177140.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK144149.1, AK052027.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-448               AC132315.4         102600-103047
            449-4141            AC132315.4         103481-107173
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 52.15 cM"
     gene            1..4141
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="lysosomal enzyme trafficking factor"
                     /db_xref="GeneID:320351"
                     /db_xref="MGI:MGI:2443862"
     exon            1..448
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /inference="alignment:Splign:2.1.0"
     CDS             369..860
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPF0694 transmembrane protein C14orf109 homolog;
                     transmembrane protein 251"
                     /codon_start=1
                     /product="lysosomal enzyme trafficking factor isoform 1"
                     /protein_id="NP_796114.1"
                     /db_xref="CCDS:CCDS49146.1"
                     /db_xref="GeneID:320351"
                     /db_xref="MGI:MGI:2443862"
                     /translation="MPKAPDCSELSDSCSLAGGTGRFSGPLHRAWRMMNFRQRMGWIG
                     VGLYLLASAAAFYYVFEINETYNRLALEHILQHPEEPREGTTWTHSLKARLLSLPFWL
                     WTIIFLIPYLQMFLFLYSCTRADPKTVGYCIIPICLAVICNRHQAFVKASNQISRLQL
                     IDT"
     exon            449..4141
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1073..1078
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="hexamer: AGTAAA"
     polyA_site      1092
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="major polyA site"
     regulatory      1230..1235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="hexamer: CATAAA"
     polyA_site      1252
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="major polyA site"
     regulatory      1520..1525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="hexamer: ATTAAA"
     polyA_site      1543
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
     regulatory      3389..3394
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="hexamer: AACAAA"
     polyA_site      3411
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
     regulatory      4118..4123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
                     /note="hexamer: ATTAAA"
     polyA_site      4141
                     /gene="Lyset"
                     /gene_synonym="D230037D09Rik; Tmem251"
ORIGIN      
        1 gaatgcggcg gtatccggga ctgcgagcgg gcgggccact caggacagga aggtgagacc
       61 cggggctgct cggcttggct gtcaggctgg cccggcgtcc ggccgggagc cgagctcggg
      121 cctctgcagg ccaagcctgg cgccactgcc cagctcagct cggcggcggg tgcagtgggg
      181 ggcgggcccg gcatctccag gtggttcccc ccgcctgggg ctccggaggg cgacggggcc
      241 cgccgccgcc gcgtgaccag ccgtagtcac gctgagctta atcactacta gctcgtgctc
      301 ccgccgccgc gctcaccttc ttcagctgcg cgacccagag ggtttctctt aaggtggctt
      361 gttctgaaat gccaaaggca cctgactgtt cagaactgag tgactcttgc tcgcttgcag
      421 ggggaacagg aagattttcg ggaccactgc acagagcatg gagaatgatg aacttccgtc
      481 agcggatggg atggattgga gtgggactgt atttgctagc aagtgcagca gcgttttatt
      541 atgtttttga aatcaatgag acttacaaca ggttggcttt ggaacacatt ctgcagcacc
      601 ctgaggagcc tcgggaagga accacatgga cacactcctt gaaagctcgg ttactttccc
      661 tgcccttttg gttgtggaca attattttct tgattccgta cttacagatg tttttgttcc
      721 tttattcttg tacgagagcg gatcccaaaa cagtgggcta ctgtattatc cccatatgct
      781 tggcagttat ctgcaatcgt catcaggcat ttgtcaaggc ttctaatcag atcagcagac
      841 tacaactgat tgacacataa cacccatcac cggtttccct tattgtaaga ctgccagcac
      901 tgtatagtgg ctgaccacaa gactgcagtt tcttcacagg tctcaggaag ttgtggtgga
      961 acgagactta aaaagtgaca agaggactga ctactttatc tgaataatga ctttttaggt
     1021 aaagcctgaa atatagtact acacaatcat gttgatgact tcagagtttg gaagtaaaat
     1081 agtggcttgt aacctgcagt ctttagaaac ttgaataagc gcctgaattt ttttatttct
     1141 attctatgtg caacatagta tgattcagaa atttttccat tggggaaaat atttccattg
     1201 tgttaaatgt aaaaaggtct gaccatgatc ataaaattta aattttatac aattactttg
     1261 cttgctttaa aaattttcag actaagacac taatcctgct ttcagtttac atggaattgg
     1321 tagaaaatta atgagatggc tatcaccttt ctgttttttt cgggggaggt gggtaggtag
     1381 gggagagtgc tggggataga atctaagcct ttgtgctttt caagacagtt actcagtgtg
     1441 ccctggctgt cctggaactt gccctgtaga tcaggctggc ttcaaattct tgcctgcccc
     1501 tgcctcccca acagctggga ttaaaggttt gtgccaccac caacccattg ctttttgtac
     1561 ttttaccact gaattacatt tccagccatg atgatggcta tttagataca cagaatttgt
     1621 tcttttcttt ttgggtaatg gctatctaaa ccactaatct atagttcttt gaaaaactat
     1681 gtaggaactg aacagatgaa atggttgaag tctagaaata acacagacac caggtgtacc
     1741 agcacagatc actgaagaga tattgttcct ttcctgggtg aaatcagggt atccagtggt
     1801 agtagtacta acacatttca caccatttta ataaccatgg cttttgagtc attgctttag
     1861 agctacaaca acaaccttat gaatgagagc agcactttag cctgatgcag ttcctcacca
     1921 ctgacacaac atcccatgct gtgtacaaac atcaactatt tacctgtgta tactgactca
     1981 taccaaaaat gaaaatgagt tttagtgaag gttttttttt ttcccaaatt gtaccatctg
     2041 ggagcttttt gtggtgctaa agatcaaact gacatcctcg aacatacaag ccaactctta
     2101 actactgtgt tgtataaccc tagtcctaaa gtttctcagc aacctcacta actgctgtat
     2161 ttgaacagag aggctaaaat tccatggcaa aatttaatct tttttggttg tatctcaaaa
     2221 gcatcagaat ctactttatg aactcctggt ggtggaccat gcccttgatc atgctagcac
     2281 ttgagaggca gagagacgaa tctctgagct ggaggccagc ctggtctaca tgaaaagttc
     2341 caggacagcc agagctacaa agacagaccc tgtcccccaa aaaacccccc ataagacaaa
     2401 aaccaaaacc cacaaactca aaaaccaact cagtttagtt gctgaaataa cacatctttt
     2461 gctataatat attcagtatt tcagggaaac agtttgacaa aactacttct gtgaatcagg
     2521 tgcatctttt gagtgctttt aatatgcatg ctgcagaaat gttttagaag tattcttcac
     2581 caactcttta ctcccaatac caaggcagct gttgctactg ggacaagtgc cattttacaa
     2641 gactgaagta ttcaggaatt aataacctgt ccatggacag ggtaagtaac tggaaaagcc
     2701 aagctaagag ccataggcag ggtatggtgg cacattcctg taatctagca ttcaggggca
     2761 gatgcaggtg gatctctgta aatctagtct atgtagtgag tgctagtaca gtcagctaca
     2821 tagggaaacc ctgcctgaaa acaacagtaa aaaatcagat gaagtaagat ttgaacccag
     2881 gatatctatc tggcttcaga ttccatctct gtacttaagg aaaaatgatt aattaatgat
     2941 aattagtatg gaagtagtag caatttattt ttatttttgt caagacaggg tttctctgtg
     3001 tagcgctggc taacctggaa cttgctctgt agaccaggtt ggctttgaac tcaaagaccc
     3061 aactgctttt gcagggatta aaggcatttc cagatataat ttattaatgg ttagtttaca
     3121 aggttaatct gctctttaaa aaaaaaatca cattccctta tctctgtttg tacaaatgtt
     3181 aagttgggtg ccatagtgta tatatggagg gcagaggaca accatgaaca tgttttctca
     3241 ggtgctatcc atttagattt tggagacagg gtctttctta ctggcctggg gcttaccaat
     3301 tctccaagga tcccagtttc cactggacca aattgcttag ctccctccaa tcccctttaa
     3361 aaaacaaaca aaaatatgtg tccttgcaaa caaacaattc ttcaaacttt accagccgtg
     3421 ccattgtcat tttatttaca accctacctg aaatgaaaca atcgcattga agttttttca
     3481 ctgaaatact tgagtgcagg cacagtaaat agattagctg cagaagtgtc tatggcactt
     3541 ttttcgctag tacacacttt tgacttagat gtcattaact ttaaagtaaa aacccccgga
     3601 agtatagaat ctgattctgt tttacaatct gtttaaggaa caaacagctt ttctttggtg
     3661 gagtcacgag ataaagtgct cacatgagta agtcaccacc gcttgaaaca aatgcgaatg
     3721 cttgtaccaa atgggtaggg ttagtctttt ccattccctg gcacaagtga ttactgtgct
     3781 gcttcattgt tcatcctttc atcacgaatg caccactgtg cattcccaca cataaagtcc
     3841 tagaaaactt tggactgtgg ttggaaatca ccatgcagtt agtgtttaaa accctgcaaa
     3901 cctcctcaca tacaataata aggtccatgg aggcgggtcc aactcttttt gtcttaaaca
     3961 ttttcagatg cttctactag ggttcaaaga actgatccac agtgttgagc atttactgaa
     4021 gattcaagta ctggagctgt gtttatcttt ggaacagaat cagaaagtgt tctgcagaaa
     4081 cttgagtcat ttccacattt tgtctacatg tcctcatatt aaatcctccc tgtgctctga
     4141 a
//
