LOCUS       NM_013589               6599 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus latent transforming growth factor beta binding protein
            2 (Ltbp2), transcript variant 2, mRNA.
ACCESSION   NM_013589
VERSION     NM_013589.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Jasmine, Baraiya,D.H., Kavya,T.T., Mandal,A., Chakraborty,S.,
            Sathish,N., Francis,C.M.R. and Binoy Joseph,D.
  TITLE     Epithelial and mesenchymal compartments of the developing bladder
            and urethra display spatially distinct gene expression patterns
  JOURNAL   Dev Biol 520, 155-170 (2025)
   PUBMED   39798644
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6599)
  AUTHORS   Bodmer,N.K., Knutsen,R.H., Roth,R.A., Castile,R.M., Brodt,M.D.,
            Gierasch,C.M., Broekelmann,T.J., Gibson,M.A., Haspel,J.A.,
            Lake,S.P., Koenitzer,J.R., Brody,S.L., Silva,M.J., Mecham,R.P. and
            Ornitz,D.M.
  TITLE     Multi-organ phenotypes in mice lacking latent TGFbeta binding
            protein 2 (LTBP2)
  JOURNAL   Dev Dyn 253 (2), 233-254 (2024)
   PUBMED   37688792
  REMARK    GeneRIF: Multi-organ phenotypes in mice lacking latent TGFbeta
            binding protein 2 (LTBP2).
            Review article
REFERENCE   4  (bases 1 to 6599)
  AUTHORS   Shi,Y., Jones,W., Beatty,W., Tan,Q., Mecham,R.P., Kumra,H.,
            Reinhardt,D.P., Gibson,M.A., Reilly,M.A., Rodriguez,J. and
            Bassnett,S.
  TITLE     Latent-transforming growth factor beta-binding protein-2 (LTBP-2)
            is required for longevity but not for development of zonular fibers
  JOURNAL   Matrix Biol 95, 15-31 (2021)
   PUBMED   33039488
  REMARK    GeneRIF: Latent-transforming growth factor beta-binding protein-2
            (LTBP-2) is required for longevity but not for development of
            zonular fibers.
REFERENCE   5  (bases 1 to 6599)
  AUTHORS   Koppes,E., Shaffer,B., Sadovsky,E., Himes,K., Barak,Y., Sadovsky,Y.
            and Chaillet,J.R.
  TITLE     Klf14 is an imprinted transcription factor that regulates placental
            growth
  JOURNAL   Placenta 88, 61-67 (2019)
   PUBMED   31675530
REFERENCE   6  (bases 1 to 6599)
  AUTHORS   Shipley,J.M., Mecham,R.P., Maus,E., Bonadio,J., Rosenbloom,J.,
            McCarthy,R.T., Baumann,M.L., Frankfater,C., Segade,F. and
            Shapiro,S.D.
  TITLE     Developmental expression of latent transforming growth factor beta
            binding protein 2 and its requirement early in mouse development
  JOURNAL   Mol Cell Biol 20 (13), 4879-4887 (2000)
   PUBMED   10848613
REFERENCE   7  (bases 1 to 6599)
  AUTHORS   Saharinen,J., Hyytiainen,M., Taipale,J. and Keski-Oja,J.
  TITLE     Latent transforming growth factor-beta binding proteins
            (LTBPs)--structural extracellular matrix proteins for targeting
            TGF-beta action
  JOURNAL   Cytokine Growth Factor Rev 10 (2), 99-117 (1999)
   PUBMED   10743502
  REMARK    Review article
REFERENCE   8  (bases 1 to 6599)
  AUTHORS   Yin,W., Fang,J., Smiley,E. and Bonadio,J.
  TITLE     8-Cysteine TGF-BP structural motifs are the site of covalent
            binding between mouse LTBP-3, LTBP-2, and latent TGF-beta 1
  JOURNAL   Biochim Biophys Acta 1383 (2), 340-350 (1998)
   PUBMED   9602168
REFERENCE   9  (bases 1 to 6599)
  AUTHORS   Fang,J., Li,X., Smiley,E., Francke,U., Mecham,R.P. and Bonadio,J.
  TITLE     Mouse latent TGF-beta binding protein-2: molecular cloning and
            developmental expression
  JOURNAL   Biochim Biophys Acta 1354 (3), 219-230 (1997)
   PUBMED   9427531
REFERENCE   10 (bases 1 to 6599)
  AUTHORS   Nakajima,Y., Miyazono,K., Kato,M., Takase,M., Yamagishi,T. and
            Nakamura,H.
  TITLE     Extracellular fibrillar structure of latent TGF beta binding
            protein-1: role in TGF beta-dependent endothelial-mesenchymal
            transformation during endocardial cushion tissue formation in mouse
            embryonic heart
  JOURNAL   J Cell Biol 136 (1), 193-204 (1997)
   PUBMED   9008713
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122310.2.
            
            On May 24, 2019 this sequence version replaced NM_013589.3.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting isoform (2) has the same
            N- and C-termini but is shorter compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC119785.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-736               AC122310.2         5179-5914
            737-807             AC122310.2         12923-12993
            808-1060            AC122310.2         27731-27983
            1061-1236           AC122310.2         49747-49922
            1237-1407           AC122310.2         50857-51027
            1408-1614           AC122310.2         51448-51654
            1615-1898           AC122310.2         52253-52536
            1899-2001           AC122310.2         53120-53222
            2002-2076           AC122310.2         65634-65708
            2077-2199           AC122310.2         68308-68430
            2200-2364           AC122310.2         71921-72085
            2365-2580           AC122310.2         72307-72522
            2581-2600           AC122310.2         73226-73245
            2601-2640           AC122310.2         74460-74499
            2641-2754           AC122310.2         75257-75370
            2755-2883           AC122310.2         77185-77313
            2884-3003           AC122310.2         82179-82298
            3004-3123           AC122310.2         86510-86629
            3124-3246           AC122310.2         87604-87726
            3247-3372           AC122310.2         87926-88051
            3373-3498           AC122310.2         88588-88713
            3499-3621           AC122310.2         89726-89848
            3622-3747           AC122310.2         89948-90073
            3748-3870           AC122310.2         90553-90675
            3871-4002           AC122310.2         91297-91428
            4003-4128           AC122310.2         91787-91912
            4129-4272           AC122310.2         92443-92586
            4273-4464           AC122310.2         93575-93766
            4465-4548           AC122310.2         94221-94304
            4549-4677           AC122310.2         95186-95314
            4678-4815           AC122310.2         95559-95696
            4816-4983           AC122310.2         95775-95942
            4984-5265           AC122310.2         96185-96466
            5266-5415           AC122310.2         96767-96916
            5416-6599           AC122310.2         97275-98458
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 39.39 cM"
     gene            1..6599
                     /gene="Ltbp2"
                     /note="latent transforming growth factor beta binding
                     protein 2"
                     /db_xref="GeneID:16997"
                     /db_xref="MGI:MGI:99502"
     exon            1..736
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..5561
                     /gene="Ltbp2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; LTBP-2"
                     /codon_start=1
                     /product="latent-transforming growth factor beta-binding
                     protein 2 isoform 2 precursor"
                     /protein_id="NP_038617.4"
                     /db_xref="CCDS:CCDS49112.2"
                     /db_xref="GeneID:16997"
                     /db_xref="MGI:MGI:99502"
                     /translation="MRAPTTARCSGCIRRVRWRGFLPLVLAVLMGTSHAQRDSIGRYE
                     PASRDANRLWHPVGSHPAAAAAKVYSLFREPDAPVPGLSPSEWNQPAQGNPGRLAEAE
                     ARRPPRTQQLRRVQPPVQTRRSHPRGQQQIAARAAPSVARLETPQRPAAARRGRLTGR
                     NVCGGQCCPGWTTSNSTNHCIKPVCQPPCQNRGSCSRPQVCICRSGFRGARCEEVIPE
                     EEFDPQNARPVPRRSVERAPGPHRSSEARGSLVTRIQPLVPPPSPPPSRRLSQPWPLQ
                     QHSGPSRTVRRYPATGANGQLMSNALPSGLELRDSSPQAAHVNHLSPPWGLNLTEKIK
                     KIKVVFTPTICKQTCARGRCANSCEKGDTTTLYSQGGHGHDPKSGFRIYFCQIPCLNG
                     GRCIGRDECWCPANSTGKFCHLPVPQPDREPAGRGSRHRTLLEGPLKQSTFTLPLSNQ
                     LASVNPSLVKVQIHHPPEASVQIHQVARVRGELDPVLEDNSVETRASRRPHGNLGHSP
                     WASNSIPARAGEAPRPPPVLSRHYGLLGQCYLSTVNGQCANPLGELTSQEDCCGSVGT
                     FWGVTSCAPCPPRPAFPVIENGQLECPQGYKRLNLSHCQDINECLTLGLCKDSECVNT
                     RGSYLCTCRPGLMLDPSRSRCVSDKAVSMQQGLCYRSLGSGTCTLPLVHRITKQICCC
                     SRVGKAWGSTCEQCPLPGTEAFREICPAGHGYTYSSSDIRLSMRKAEEEELASPLREQ
                     TEQSTAPPPGQAERQPLRAATATWIEAETLPDKGDSRAVQITTSAPHLPARVPGDATG
                     RPAPSLPGQGIPESPAEEQVIPSSDVLVTHSPPDFDPCFAGASNICGPGTCVSLPNGY
                     RCVCSPGYQLHPSQDYCTDIDECEQPGVCSGGRCSNTEGSYHCECDRGYIMVRKGHCQ
                     DINECRHPGTCPDGRCVNSPGSYTCLACEEGYVGQSGSCVDVNECLTPGICTHGRCIN
                     MEGSFRCSCEPGYEVTPDKKGCRDVDECASRASCPTGLCLNTEGSFTCSACQSGYWVN
                     EDGTACEDLDECAFPGVCPTGVCTNTVGSFSCKDCDRGYRPNPLGNRCEDVDECEGPQ
                     SSCRGGECKNTEGSYQCLCHQGFQLVNGTMCEDVNECVGEEHCAPHGECLNSLGSFFC
                     LCAPGFASAEGGTRCQDVDECAATDPCPGGHCVNTEGSFSCLCETGFQPSPDSGECLD
                     IDECEDREDPVCGAWRCENSPGSYRCILDCQPGFYVAPNGDCIDIDECANDTVCGNHG
                     FCDNTDGSFRCLCDQGFETSPSGWECVDVNECELMMAVCGDALCENVEGSFLCLCASD
                     LEEYDAEEGHCRPRVAGAQRIPEVRTEDQAPSLIRMECYSEHNGGPPCSQILGQNSTQ
                     AECCCTQGARWGKACAPCPSEDSVEFSQLCPSGQGYIPVEGAWTFGQTMYTDADECVL
                     FGPALCQNGRCLNIVPGYICLCNPGYHYDASSRKCQDHNECQDLACENGECVNTEGSF
                     HCLCNPPLTLDLSGQRCVNSTSSTEDFPDHDIHMDICWKKVTNDVCSQPLRGHHTTYT
                     ECCCQDGEAWSQQCALCPPRSSEVYAQLCNVARIEAERGAGIHFRPGYEYGPGLDDLP
                     ENLYGPDGAPFYNYLGPEDTAPEPPFSNPASQPGDNTPVLEPPLQPSELQPHYLASHS
                     EPLASFEGLQAEECGILNGCENGRCVRVREGYTCDCFEGFQLDAAHMACVDVNECEDL
                     NGPAALCAHGHCENTEGSYRCHCSPGYVAEPGPPHCAAKE"
     exon            737..807
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            808..1060
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1236
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1407
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1614
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1898
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2001
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2076
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..2199
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2364
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..2580
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..2600
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2640
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2641..2754
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2883
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2884..3003
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3004..3123
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3246
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3247..3372
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3373..3498
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3499..3621
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3622..3747
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3748..3870
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3871..4002
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4003..4128
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4129..4272
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4273..4464
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4465..4548
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4549..4677
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4678..4815
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4816..4983
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4984..5265
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            5266..5415
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            5416..6599
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6581..6586
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ltbp2"
                     /note="hexamer: AATAAA"
     polyA_site      6599
                     /gene="Ltbp2"
                     /note="major polyA site"
ORIGIN      
        1 ccaaaaataa aacccttcgg ttccccttca gacggcggca gtgcgggagc gaaggtgcgg
       61 caggctcggg ctcggtgcgc agcccgggca gctcacgcca tacagccctt acctaggaac
      121 ccctggaccc ccaaacggct gtttcagagg tcccctagac gggaaggggc aagtcgccag
      181 gcgggtctgt ggagcgaacg atggaaagca cctccccgcg aggtctccgg tgcccacagc
      241 tctgcagcca ctctggcgcc atgagagcgc cgaccaccgc tcgctgctcc ggatgcatcc
      301 gacgggtgcg ttggaggggc ttcctgccac ttgtcctggc tgtcttgatg gggacaagtc
      361 atgcccaacg ggattccata gggagatacg aaccagctag cagggatgcg aatcggttgt
      421 ggcaccccgt gggcagccac cccgcagcgg ctgcagccaa ggtgtacagt ctgttccgag
      481 agcctgacgc gccggtcccc ggcttgtcgc cctctgagtg gaaccagccg gcccagggga
      541 accctgggag gctcgcagag gccgaggcca ggaggccacc ccgaacccag cagctgcgtc
      601 gagtccagcc acctgtccag actcggagaa gccatccccg gggccagcag cagatagcag
      661 ccagggctgc accttctgtc gcgcgcctgg aaacccctca gcgacccgcg gctgcacggc
      721 gagggcggct caccgggaga aatgtctgcg ggggacagtg ctgcccagga tggacaacat
      781 caaacagcac caaccactgt atcaaacctg tgtgtcagcc tccctgtcag aaccgaggct
      841 cctgcagcag gccccaggtc tgtatctgcc gttctggctt ccgtggggcg cgctgtgagg
      901 aggtcatccc tgaggaggaa tttgaccctc agaatgccag gcctgtgccc agacgctcag
      961 tggagagagc acccggtcct cacagaagca gtgaggccag aggaagtcta gtgaccagaa
     1021 tacagccgct ggtaccacca ccatcaccac ctccatctcg gcgcctcagc cagccctggc
     1081 ccctgcagca gcactcaggg ccgtccagga cagttcgtcg gtatccggcc actggtgcca
     1141 atggccagct gatgtccaac gctttgcctt caggactcga gctgagagac agcagcccac
     1201 aggcagcaca tgtgaaccat ctctcacccc cctgggggct gaacctcacc gagaaaatca
     1261 agaaaatcaa agtcgtcttc acccccacca tctgcaagca gacctgtgcc cggggacgct
     1321 gtgccaacag ctgtgagaag ggtgacacca ccaccttgta cagtcagggt ggccatgggc
     1381 atgaccccaa gtctggcttc cgtatctatt tctgccaaat cccctgcctg aatggtggcc
     1441 gctgcatcgg ccgggacgag tgctggtgtc cagccaactc cacaggaaag ttctgccatc
     1501 tgcctgtccc gcagccagac agggaacctg cagggcgagg ttcccggcac agaaccctgc
     1561 tggaaggtcc cctgaagcaa tccaccttca cgctgcctct ctctaaccag ctcgcctctg
     1621 tgaacccctc gctggtgaag gtgcaaattc atcacccgcc tgaggcctct gtgcagattc
     1681 accaggtggc ccgggtccgg ggtgagctgg accccgtgct ggaggacaac agtgtggaga
     1741 ccagagcctc tcgtcgcccc cacggcaacc taggccacag cccctgggcc agcaacagca
     1801 tacccgctcg ggccggagag gcccctcggc caccaccagt gctgtctagg cattatggac
     1861 ttctgggcca gtgttacctg agcacggtga atggacagtg tgctaacccc ctaggggagc
     1921 tgacttctca ggaggactgc tgtggcagtg tggggacctt ctggggggtg acctcctgtg
     1981 ctccctgccc acccagacca gccttcccag tgattgaaaa tggccagctg gagtgtcccc
     2041 aaggatacaa gagactgaac ctcagccact gccaagatat caatgagtgc ctgaccctgg
     2101 gcctctgcaa ggactcggag tgcgtgaaca ccaggggcag ctacctgtgc acctgcaggc
     2161 ctggcctcat gctggatccg tcaaggagcc gctgcgtatc ggacaaggct gtctccatgc
     2221 agcagggact atgctaccgg tcactggggt ctggtacctg caccctgcct ttggttcatc
     2281 ggatcaccaa gcagatatgc tgctgcagcc gtgtgggcaa agcctggggt agcacatgtg
     2341 aacagtgtcc cctgcctggc acagaagcct tcagggagat ctgccctgct ggccatggct
     2401 acacctactc gagctcagac atccgcctgt ctatgaggaa agccgaagaa gaggaactgg
     2461 ctagcccctt aagggagcag acagagcaga gcactgcacc cccacctggg caagcagaga
     2521 ggcaaccact ccgggcagcc accgccacct ggattgaggc tgagaccctc cctgacaaag
     2581 gtgactctcg ggctgttcag atcacaacca gtgctcccca cctacctgcc cgggtaccag
     2641 gggatgccac tggaagacca gcaccatcct tgcctggaca gggcattcca gagagtccag
     2701 cagaagagca agtgattccc tccagtgatg tcttggtgac acacagcccc ccagactttg
     2761 atccatgttt tgctggagcc tccaacatct gtggccctgg gacctgtgtg agcctcccaa
     2821 atggatacag atgtgtctgc agccctggct accagctaca ccccagccaa gactactgta
     2881 ctgatatcga tgagtgtgag cagcccgggg tgtgcagtgg tgggcgatgc agcaacacgg
     2941 agggctcgta ccactgcgag tgtgatcggg gctacatcat ggtcaggaaa ggacactgtc
     3001 aagatatcaa cgaatgccgt caccctggta cctgccctga tgggagatgc gtcaactccc
     3061 ctggctccta cacttgtctg gcctgtgagg agggctatgt aggccagagt gggagctgtg
     3121 tagatgtcaa tgagtgtctg acccctggga tatgtaccca tggaaggtgc atcaacatgg
     3181 aaggctcctt tagatgctcc tgtgagccgg gctatgaggt caccccagac aagaagggct
     3241 gccgagatgt ggacgagtgt gccagccgag cctcgtgccc caccggcctc tgcctcaaca
     3301 cggagggctc cttcacctgc tcagcctgtc agagcgggta ctgggtgaac gaagatggca
     3361 ctgcctgtga agacttggat gaatgtgcct tccctggagt ctgccccaca ggcgtctgca
     3421 ccaatactgt aggctccttc tcctgcaagg actgtgacag gggctaccgg cccaaccccc
     3481 tgggcaacag atgcgaagat gtggatgagt gtgaaggtcc ccaaagcagc tgccggggag
     3541 gcgaatgcaa gaacacagaa ggttcctacc aatgcctctg tcaccagggc ttccagctgg
     3601 tcaatggcac catgtgtgag gacgtgaatg agtgtgttgg ggaagagcat tgtgctcctc
     3661 acggcgagtg cctcaacagc ctgggctcct tcttctgcct ctgtgcaccc ggctttgcta
     3721 gtgctgaggg gggcaccaga tgccaggatg ttgatgaatg tgcagccaca gacccgtgtc
     3781 cgggaggaca ctgtgtcaac acagagggct ccttcagctg tctgtgtgag actggcttcc
     3841 agccctcccc agacagcgga gaatgtttgg atattgatga gtgtgaggac cgtgaagacc
     3901 cggtgtgcgg agcctggagg tgtgagaaca gtcctggttc ctaccgctgc atcctggact
     3961 gccagcctgg cttctatgtg gcgccaaatg gagactgcat tgacatagat gaatgtgcca
     4021 atgacactgt gtgtgggaac catggcttct gtgacaacac ggacggctcc ttccgctgcc
     4081 tgtgtgacca gggcttcgag acctcaccat caggctggga gtgtgttgat gtgaacgagt
     4141 gtgagctcat gatggcagtg tgtggggatg cgctctgtga gaacgtggaa ggctccttcc
     4201 tgtgcctttg cgccagtgac cttgaggagt acgacgcaga agaaggacac tgccgtcctc
     4261 gggtggctgg agctcagaga atcccagagg tccggacaga ggaccaggct ccaagcctta
     4321 tccgcatgga atgctactct gaacacaatg gtggtcctcc ctgctctcaa atcctgggcc
     4381 agaactccac acaggccgag tgctgctgca ctcagggtgc cagatgggga aaggcctgtg
     4441 cgccctgccc atctgaggac tcagttgaat tcagtcagct ctgccccagt ggtcaaggtt
     4501 acatcccagt ggaaggagcc tggacatttg gacaaaccat gtatacagat gccgatgaat
     4561 gtgtactgtt tgggcctgct ctctgccaga atggccgatg cctcaacata gtgcctggct
     4621 acatttgcct gtgcaaccct ggctaccact atgatgcctc cagcaggaag tgccaggatc
     4681 acaacgaatg ccaggacttg gcctgtgaga acggtgagtg tgtgaacaca gaaggctcct
     4741 tccattgcct ctgcaatccc cccctcaccc tagacctcag tgggcagcgc tgtgtgaaca
     4801 gcaccagcag cacggaggac ttccctgacc atgacatcca catggacatc tgctggaaaa
     4861 aagtcaccaa tgatgtgtgc agccagccct tgcgtgggca ccataccacc tatacagaat
     4921 gctgctgcca agatggggag gcctggagcc agcaatgcgc tctgtgcccg cccaggagct
     4981 ctgaggtcta cgctcagctg tgcaacgtgg ctcggattga ggcagagcgc ggagcaggga
     5041 tccacttccg gccaggctat gagtatggcc ctggcctgga cgatctgcct gaaaacctct
     5101 acggcccaga tggggctccc ttctataact acctaggccc cgaggacact gcccctgagc
     5161 ctcccttctc caacccagcc agccagccgg gagacaacac acctgtcctt gagcctcctc
     5221 tgcagccctc tgaacttcag cctcactatc tagccagcca ctcagaaccc ctggcctcct
     5281 tcgaaggcct tcaggctgag gaatgtggca tcctgaatgg ctgtgagaat ggccgctgcg
     5341 tgcgtgtgcg ggagggctac acttgcgact gctttgaggg cttccagctg gatgcggccc
     5401 acatggcctg tgtggatgtg aacgagtgtg aagacttgaa cgggcctgca gcactctgtg
     5461 cacacggtca ctgtgagaac acagagggtt cctatcgctg ccactgttcg ccaggttacg
     5521 tggcagagcc aggcccccca cactgtgcgg ccaaggagta gaagtgaggg cttgtgtggg
     5581 cagctatctg gaaaatggct tcagccatag gccgtggact taagattgct tccctagctg
     5641 ggaagacatc gcgctgggaa gatgacgtga tgacatcagg cccgggctct ggacccagtt
     5701 ctgccagcct tgccaaattt ttatttcttc cagcttaact ctgggtgtga gcttccatca
     5761 ctgcctctat gccattgctt ggctcagaca ccacaaatat cttaatgctt cagccactgg
     5821 ccatgagaca cgcccaccag tctgtccccg ggaccacact atagggcact catcaggctg
     5881 tgctcttagg ctgtgcaaac attgcaggta cccctttcca gttgcgagcc acacatcatc
     5941 ctagttgttc tgtaatgggg acagtggctg catccgccca gggatggtct cttcacagtc
     6001 tgtggagcaa gagagggttt ggggagtagc tcctgtcacc tctcagaacc aacaccacca
     6061 ctcggccagg ctggcatgag ctctttttgc cacatctcca tctcttagac agttctgcag
     6121 cccctcatct cagaggatga agactaatac taactcgctg agtggtaaga aatacaaagg
     6181 acgaggaaca gtgagcctga gaaagtacgg caagagagtg atgtggaaaa catggcgagc
     6241 tgatacgaaa ggaggagcca agagttaaag aaatctaaaa acactgagtt gctttgggca
     6301 aataagtact tgaagcccct tagaggctct gtgtgcctgc agtgaggctt gtcataagct
     6361 ctggcgccca tatgcaaagg cctttcttag gcacatctat ccctatgggg aaacccaaat
     6421 agatgggcta acaatataaa ggtctccttt tcagctggtc ttggcagagg aggccaggtt
     6481 ttcaaagttg tttatctcct ccactcagaa gcacttgccc ttgttccctc taagaaatgc
     6541 acttccatac aagctagctg ggggttcagg agcatggggg aataaaatgt tcatctgga
//
