LOCUS       NM_001370743            6728 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus latent transforming growth factor beta binding protein
            2 (Ltbp2), transcript variant 1, mRNA.
ACCESSION   NM_001370743 XM_006515501
VERSION     NM_001370743.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Jasmine, Baraiya,D.H., Kavya,T.T., Mandal,A., Chakraborty,S.,
            Sathish,N., Francis,C.M.R. and Binoy Joseph,D.
  TITLE     Epithelial and mesenchymal compartments of the developing bladder
            and urethra display spatially distinct gene expression patterns
  JOURNAL   Dev Biol 520, 155-170 (2025)
   PUBMED   39798644
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6728)
  AUTHORS   Bodmer,N.K., Knutsen,R.H., Roth,R.A., Castile,R.M., Brodt,M.D.,
            Gierasch,C.M., Broekelmann,T.J., Gibson,M.A., Haspel,J.A.,
            Lake,S.P., Koenitzer,J.R., Brody,S.L., Silva,M.J., Mecham,R.P. and
            Ornitz,D.M.
  TITLE     Multi-organ phenotypes in mice lacking latent TGFbeta binding
            protein 2 (LTBP2)
  JOURNAL   Dev Dyn 253 (2), 233-254 (2024)
   PUBMED   37688792
  REMARK    GeneRIF: Multi-organ phenotypes in mice lacking latent TGFbeta
            binding protein 2 (LTBP2).
            Review article
REFERENCE   4  (bases 1 to 6728)
  AUTHORS   Shi,Y., Jones,W., Beatty,W., Tan,Q., Mecham,R.P., Kumra,H.,
            Reinhardt,D.P., Gibson,M.A., Reilly,M.A., Rodriguez,J. and
            Bassnett,S.
  TITLE     Latent-transforming growth factor beta-binding protein-2 (LTBP-2)
            is required for longevity but not for development of zonular fibers
  JOURNAL   Matrix Biol 95, 15-31 (2021)
   PUBMED   33039488
  REMARK    GeneRIF: Latent-transforming growth factor beta-binding protein-2
            (LTBP-2) is required for longevity but not for development of
            zonular fibers.
REFERENCE   5  (bases 1 to 6728)
  AUTHORS   Koppes,E., Shaffer,B., Sadovsky,E., Himes,K., Barak,Y., Sadovsky,Y.
            and Chaillet,J.R.
  TITLE     Klf14 is an imprinted transcription factor that regulates placental
            growth
  JOURNAL   Placenta 88, 61-67 (2019)
   PUBMED   31675530
REFERENCE   6  (bases 1 to 6728)
  AUTHORS   Shipley,J.M., Mecham,R.P., Maus,E., Bonadio,J., Rosenbloom,J.,
            McCarthy,R.T., Baumann,M.L., Frankfater,C., Segade,F. and
            Shapiro,S.D.
  TITLE     Developmental expression of latent transforming growth factor beta
            binding protein 2 and its requirement early in mouse development
  JOURNAL   Mol Cell Biol 20 (13), 4879-4887 (2000)
   PUBMED   10848613
REFERENCE   7  (bases 1 to 6728)
  AUTHORS   Saharinen,J., Hyytiainen,M., Taipale,J. and Keski-Oja,J.
  TITLE     Latent transforming growth factor-beta binding proteins
            (LTBPs)--structural extracellular matrix proteins for targeting
            TGF-beta action
  JOURNAL   Cytokine Growth Factor Rev 10 (2), 99-117 (1999)
   PUBMED   10743502
  REMARK    Review article
REFERENCE   8  (bases 1 to 6728)
  AUTHORS   Yin,W., Fang,J., Smiley,E. and Bonadio,J.
  TITLE     8-Cysteine TGF-BP structural motifs are the site of covalent
            binding between mouse LTBP-3, LTBP-2, and latent TGF-beta 1
  JOURNAL   Biochim Biophys Acta 1383 (2), 340-350 (1998)
   PUBMED   9602168
REFERENCE   9  (bases 1 to 6728)
  AUTHORS   Fang,J., Li,X., Smiley,E., Francke,U., Mecham,R.P. and Bonadio,J.
  TITLE     Mouse latent TGF-beta binding protein-2: molecular cloning and
            developmental expression
  JOURNAL   Biochim Biophys Acta 1354 (3), 219-230 (1997)
   PUBMED   9427531
REFERENCE   10 (bases 1 to 6728)
  AUTHORS   Nakajima,Y., Miyazono,K., Kato,M., Takase,M., Yamagishi,T. and
            Nakamura,H.
  TITLE     Extracellular fibrillar structure of latent TGF beta binding
            protein-1: role in TGF beta-dependent endothelial-mesenchymal
            transformation during endocardial cushion tissue formation in mouse
            embryonic heart
  JOURNAL   J Cell Biol 136 (1), 193-204 (1997)
   PUBMED   9008713
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122310.2.
            
            On May 24, 2019 this sequence version replaced XM_006515501.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            inferred exon combination        :: based on alignments, homology
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-736               AC122310.2         5179-5914
            737-807             AC122310.2         12923-12993
            808-1060            AC122310.2         27731-27983
            1061-1236           AC122310.2         49747-49922
            1237-1407           AC122310.2         50857-51027
            1408-1614           AC122310.2         51448-51654
            1615-1898           AC122310.2         52253-52536
            1899-2001           AC122310.2         53120-53222
            2002-2076           AC122310.2         65634-65708
            2077-2199           AC122310.2         68308-68430
            2200-2364           AC122310.2         71921-72085
            2365-2580           AC122310.2         72307-72522
            2581-2600           AC122310.2         73226-73245
            2601-2640           AC122310.2         74460-74499
            2641-2754           AC122310.2         75257-75370
            2755-2883           AC122310.2         77185-77313
            2884-3012           AC122310.2         77755-77883
            3013-3132           AC122310.2         82179-82298
            3133-3252           AC122310.2         86510-86629
            3253-3375           AC122310.2         87604-87726
            3376-3501           AC122310.2         87926-88051
            3502-3627           AC122310.2         88588-88713
            3628-3750           AC122310.2         89726-89848
            3751-3876           AC122310.2         89948-90073
            3877-3999           AC122310.2         90553-90675
            4000-4131           AC122310.2         91297-91428
            4132-4257           AC122310.2         91787-91912
            4258-4401           AC122310.2         92443-92586
            4402-4593           AC122310.2         93575-93766
            4594-4677           AC122310.2         94221-94304
            4678-4806           AC122310.2         95186-95314
            4807-4944           AC122310.2         95559-95696
            4945-5112           AC122310.2         95775-95942
            5113-5394           AC122310.2         96185-96466
            5395-5544           AC122310.2         96767-96916
            5545-6728           AC122310.2         97275-98458
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 39.39 cM"
     gene            1..6728
                     /gene="Ltbp2"
                     /note="latent transforming growth factor beta binding
                     protein 2"
                     /db_xref="GeneID:16997"
                     /db_xref="MGI:MGI:99502"
     exon            1..736
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..5690
                     /gene="Ltbp2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; LTBP-2"
                     /codon_start=1
                     /product="latent-transforming growth factor beta-binding
                     protein 2 isoform 1 precursor"
                     /protein_id="NP_001357672.1"
                     /db_xref="CCDS:CCDS88370.1"
                     /db_xref="GeneID:16997"
                     /db_xref="MGI:MGI:99502"
                     /translation="MRAPTTARCSGCIRRVRWRGFLPLVLAVLMGTSHAQRDSIGRYE
                     PASRDANRLWHPVGSHPAAAAAKVYSLFREPDAPVPGLSPSEWNQPAQGNPGRLAEAE
                     ARRPPRTQQLRRVQPPVQTRRSHPRGQQQIAARAAPSVARLETPQRPAAARRGRLTGR
                     NVCGGQCCPGWTTSNSTNHCIKPVCQPPCQNRGSCSRPQVCICRSGFRGARCEEVIPE
                     EEFDPQNARPVPRRSVERAPGPHRSSEARGSLVTRIQPLVPPPSPPPSRRLSQPWPLQ
                     QHSGPSRTVRRYPATGANGQLMSNALPSGLELRDSSPQAAHVNHLSPPWGLNLTEKIK
                     KIKVVFTPTICKQTCARGRCANSCEKGDTTTLYSQGGHGHDPKSGFRIYFCQIPCLNG
                     GRCIGRDECWCPANSTGKFCHLPVPQPDREPAGRGSRHRTLLEGPLKQSTFTLPLSNQ
                     LASVNPSLVKVQIHHPPEASVQIHQVARVRGELDPVLEDNSVETRASRRPHGNLGHSP
                     WASNSIPARAGEAPRPPPVLSRHYGLLGQCYLSTVNGQCANPLGELTSQEDCCGSVGT
                     FWGVTSCAPCPPRPAFPVIENGQLECPQGYKRLNLSHCQDINECLTLGLCKDSECVNT
                     RGSYLCTCRPGLMLDPSRSRCVSDKAVSMQQGLCYRSLGSGTCTLPLVHRITKQICCC
                     SRVGKAWGSTCEQCPLPGTEAFREICPAGHGYTYSSSDIRLSMRKAEEEELASPLREQ
                     TEQSTAPPPGQAERQPLRAATATWIEAETLPDKGDSRAVQITTSAPHLPARVPGDATG
                     RPAPSLPGQGIPESPAEEQVIPSSDVLVTHSPPDFDPCFAGASNICGPGTCVSLPNGY
                     RCVCSPGYQLHPSQDYCTDDNECMRNPCEGRGRCVNSVGSYSCLCYPGYTLVTLRDTQ
                     ECQDIDECEQPGVCSGGRCSNTEGSYHCECDRGYIMVRKGHCQDINECRHPGTCPDGR
                     CVNSPGSYTCLACEEGYVGQSGSCVDVNECLTPGICTHGRCINMEGSFRCSCEPGYEV
                     TPDKKGCRDVDECASRASCPTGLCLNTEGSFTCSACQSGYWVNEDGTACEDLDECAFP
                     GVCPTGVCTNTVGSFSCKDCDRGYRPNPLGNRCEDVDECEGPQSSCRGGECKNTEGSY
                     QCLCHQGFQLVNGTMCEDVNECVGEEHCAPHGECLNSLGSFFCLCAPGFASAEGGTRC
                     QDVDECAATDPCPGGHCVNTEGSFSCLCETGFQPSPDSGECLDIDECEDREDPVCGAW
                     RCENSPGSYRCILDCQPGFYVAPNGDCIDIDECANDTVCGNHGFCDNTDGSFRCLCDQ
                     GFETSPSGWECVDVNECELMMAVCGDALCENVEGSFLCLCASDLEEYDAEEGHCRPRV
                     AGAQRIPEVRTEDQAPSLIRMECYSEHNGGPPCSQILGQNSTQAECCCTQGARWGKAC
                     APCPSEDSVEFSQLCPSGQGYIPVEGAWTFGQTMYTDADECVLFGPALCQNGRCLNIV
                     PGYICLCNPGYHYDASSRKCQDHNECQDLACENGECVNTEGSFHCLCNPPLTLDLSGQ
                     RCVNSTSSTEDFPDHDIHMDICWKKVTNDVCSQPLRGHHTTYTECCCQDGEAWSQQCA
                     LCPPRSSEVYAQLCNVARIEAERGAGIHFRPGYEYGPGLDDLPENLYGPDGAPFYNYL
                     GPEDTAPEPPFSNPASQPGDNTPVLEPPLQPSELQPHYLASHSEPLASFEGLQAEECG
                     ILNGCENGRCVRVREGYTCDCFEGFQLDAAHMACVDVNECEDLNGPAALCAHGHCENT
                     EGSYRCHCSPGYVAEPGPPHCAAKE"
     exon            737..807
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            808..1060
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1236
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1407
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1614
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1898
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2001
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2076
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2077..2199
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2364
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..2580
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..2600
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2640
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2641..2754
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2883
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2884..3012
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3013..3132
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3133..3252
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3253..3375
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3376..3501
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3502..3627
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3628..3750
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3751..3876
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3877..3999
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4000..4131
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4132..4257
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4258..4401
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4402..4593
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4594..4677
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4678..4806
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4807..4944
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4945..5112
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            5113..5394
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            5395..5544
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     exon            5545..6728
                     /gene="Ltbp2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6710..6715
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ltbp2"
                     /note="hexamer: AATAAA"
     polyA_site      6728
                     /gene="Ltbp2"
                     /note="major polyA site"
ORIGIN      
        1 ccaaaaataa aacccttcgg ttccccttca gacggcggca gtgcgggagc gaaggtgcgg
       61 caggctcggg ctcggtgcgc agcccgggca gctcacgcca tacagccctt acctaggaac
      121 ccctggaccc ccaaacggct gtttcagagg tcccctagac gggaaggggc aagtcgccag
      181 gcgggtctgt ggagcgaacg atggaaagca cctccccgcg aggtctccgg tgcccacagc
      241 tctgcagcca ctctggcgcc atgagagcgc cgaccaccgc tcgctgctcc ggatgcatcc
      301 gacgggtgcg ttggaggggc ttcctgccac ttgtcctggc tgtcttgatg gggacaagtc
      361 atgcccaacg ggattccata gggagatacg aaccagctag cagggatgcg aatcggttgt
      421 ggcaccccgt gggcagccac cccgcagcgg ctgcagccaa ggtgtacagt ctgttccgag
      481 agcctgacgc gccggtcccc ggcttgtcgc cctctgagtg gaaccagccg gcccagggga
      541 accctgggag gctcgcagag gccgaggcca ggaggccacc ccgaacccag cagctgcgtc
      601 gagtccagcc acctgtccag actcggagaa gccatccccg gggccagcag cagatagcag
      661 ccagggctgc accttctgtc gcgcgcctgg aaacccctca gcgacccgcg gctgcacggc
      721 gagggcggct caccgggaga aatgtctgcg ggggacagtg ctgcccagga tggacaacat
      781 caaacagcac caaccactgt atcaaacctg tgtgtcagcc tccctgtcag aaccgaggct
      841 cctgcagcag gccccaggtc tgtatctgcc gttctggctt ccgtggggcg cgctgtgagg
      901 aggtcatccc tgaggaggaa tttgaccctc agaatgccag gcctgtgccc agacgctcag
      961 tggagagagc acccggtcct cacagaagca gtgaggccag aggaagtcta gtgaccagaa
     1021 tacagccgct ggtaccacca ccatcaccac ctccatctcg gcgcctcagc cagccctggc
     1081 ccctgcagca gcactcaggg ccgtccagga cagttcgtcg gtatccggcc actggtgcca
     1141 atggccagct gatgtccaac gctttgcctt caggactcga gctgagagac agcagcccac
     1201 aggcagcaca tgtgaaccat ctctcacccc cctgggggct gaacctcacc gagaaaatca
     1261 agaaaatcaa agtcgtcttc acccccacca tctgcaagca gacctgtgcc cggggacgct
     1321 gtgccaacag ctgtgagaag ggtgacacca ccaccttgta cagtcagggt ggccatgggc
     1381 atgaccccaa gtctggcttc cgtatctatt tctgccaaat cccctgcctg aatggtggcc
     1441 gctgcatcgg ccgggacgag tgctggtgtc cagccaactc cacaggaaag ttctgccatc
     1501 tgcctgtccc gcagccagac agggaacctg cagggcgagg ttcccggcac agaaccctgc
     1561 tggaaggtcc cctgaagcaa tccaccttca cgctgcctct ctctaaccag ctcgcctctg
     1621 tgaacccctc gctggtgaag gtgcaaattc atcacccgcc tgaggcctct gtgcagattc
     1681 accaggtggc ccgggtccgg ggtgagctgg accccgtgct ggaggacaac agtgtggaga
     1741 ccagagcctc tcgtcgcccc cacggcaacc taggccacag cccctgggcc agcaacagca
     1801 tacccgctcg ggccggagag gcccctcggc caccaccagt gctgtctagg cattatggac
     1861 ttctgggcca gtgttacctg agcacggtga atggacagtg tgctaacccc ctaggggagc
     1921 tgacttctca ggaggactgc tgtggcagtg tggggacctt ctggggggtg acctcctgtg
     1981 ctccctgccc acccagacca gccttcccag tgattgaaaa tggccagctg gagtgtcccc
     2041 aaggatacaa gagactgaac ctcagccact gccaagatat caatgagtgc ctgaccctgg
     2101 gcctctgcaa ggactcggag tgcgtgaaca ccaggggcag ctacctgtgc acctgcaggc
     2161 ctggcctcat gctggatccg tcaaggagcc gctgcgtatc ggacaaggct gtctccatgc
     2221 agcagggact atgctaccgg tcactggggt ctggtacctg caccctgcct ttggttcatc
     2281 ggatcaccaa gcagatatgc tgctgcagcc gtgtgggcaa agcctggggt agcacatgtg
     2341 aacagtgtcc cctgcctggc acagaagcct tcagggagat ctgccctgct ggccatggct
     2401 acacctactc gagctcagac atccgcctgt ctatgaggaa agccgaagaa gaggaactgg
     2461 ctagcccctt aagggagcag acagagcaga gcactgcacc cccacctggg caagcagaga
     2521 ggcaaccact ccgggcagcc accgccacct ggattgaggc tgagaccctc cctgacaaag
     2581 gtgactctcg ggctgttcag atcacaacca gtgctcccca cctacctgcc cgggtaccag
     2641 gggatgccac tggaagacca gcaccatcct tgcctggaca gggcattcca gagagtccag
     2701 cagaagagca agtgattccc tccagtgatg tcttggtgac acacagcccc ccagactttg
     2761 atccatgttt tgctggagcc tccaacatct gtggccctgg gacctgtgtg agcctcccaa
     2821 atggatacag atgtgtctgc agccctggct accagctaca ccccagccaa gactactgta
     2881 ctgatgacaa cgagtgtatg aggaacccct gtgaaggaag agggcgctgt gtcaacagtg
     2941 tgggctccta ctcctgcctc tgctatcctg gctacacact agtcaccctc agagacacac
     3001 aggagtgcca agatatcgat gagtgtgagc agcccggggt gtgcagtggt gggcgatgca
     3061 gcaacacgga gggctcgtac cactgcgagt gtgatcgggg ctacatcatg gtcaggaaag
     3121 gacactgtca agatatcaac gaatgccgtc accctggtac ctgccctgat gggagatgcg
     3181 tcaactcccc tggctcctac acttgtctgg cctgtgagga gggctatgta ggccagagtg
     3241 ggagctgtgt agatgtcaat gagtgtctga cccctgggat atgtacccat ggaaggtgca
     3301 tcaacatgga aggctccttt agatgctcct gtgagccggg ctatgaggtc accccagaca
     3361 agaagggctg ccgagatgtg gacgagtgtg ccagccgagc ctcgtgcccc accggcctct
     3421 gcctcaacac ggagggctcc ttcacctgct cagcctgtca gagcgggtac tgggtgaacg
     3481 aagatggcac tgcctgtgaa gacttggatg aatgtgcctt ccctggagtc tgccccacag
     3541 gcgtctgcac caatactgta ggctccttct cctgcaagga ctgtgacagg ggctaccggc
     3601 ccaaccccct gggcaacaga tgcgaagatg tggatgagtg tgaaggtccc caaagcagct
     3661 gccggggagg cgaatgcaag aacacagaag gttcctacca atgcctctgt caccagggct
     3721 tccagctggt caatggcacc atgtgtgagg acgtgaatga gtgtgttggg gaagagcatt
     3781 gtgctcctca cggcgagtgc ctcaacagcc tgggctcctt cttctgcctc tgtgcacccg
     3841 gctttgctag tgctgagggg ggcaccagat gccaggatgt tgatgaatgt gcagccacag
     3901 acccgtgtcc gggaggacac tgtgtcaaca cagagggctc cttcagctgt ctgtgtgaga
     3961 ctggcttcca gccctcccca gacagcggag aatgtttgga tattgatgag tgtgaggacc
     4021 gtgaagaccc ggtgtgcgga gcctggaggt gtgagaacag tcctggttcc taccgctgca
     4081 tcctggactg ccagcctggc ttctatgtgg cgccaaatgg agactgcatt gacatagatg
     4141 aatgtgccaa tgacactgtg tgtgggaacc atggcttctg tgacaacacg gacggctcct
     4201 tccgctgcct gtgtgaccag ggcttcgaga cctcaccatc aggctgggag tgtgttgatg
     4261 tgaacgagtg tgagctcatg atggcagtgt gtggggatgc gctctgtgag aacgtggaag
     4321 gctccttcct gtgcctttgc gccagtgacc ttgaggagta cgacgcagaa gaaggacact
     4381 gccgtcctcg ggtggctgga gctcagagaa tcccagaggt ccggacagag gaccaggctc
     4441 caagccttat ccgcatggaa tgctactctg aacacaatgg tggtcctccc tgctctcaaa
     4501 tcctgggcca gaactccaca caggccgagt gctgctgcac tcagggtgcc agatggggaa
     4561 aggcctgtgc gccctgccca tctgaggact cagttgaatt cagtcagctc tgccccagtg
     4621 gtcaaggtta catcccagtg gaaggagcct ggacatttgg acaaaccatg tatacagatg
     4681 ccgatgaatg tgtactgttt gggcctgctc tctgccagaa tggccgatgc ctcaacatag
     4741 tgcctggcta catttgcctg tgcaaccctg gctaccacta tgatgcctcc agcaggaagt
     4801 gccaggatca caacgaatgc caggacttgg cctgtgagaa cggtgagtgt gtgaacacag
     4861 aaggctcctt ccattgcctc tgcaatcccc ccctcaccct agacctcagt gggcagcgct
     4921 gtgtgaacag caccagcagc acggaggact tccctgacca tgacatccac atggacatct
     4981 gctggaaaaa agtcaccaat gatgtgtgca gccagccctt gcgtgggcac cataccacct
     5041 atacagaatg ctgctgccaa gatggggagg cctggagcca gcaatgcgct ctgtgcccgc
     5101 ccaggagctc tgaggtctac gctcagctgt gcaacgtggc tcggattgag gcagagcgcg
     5161 gagcagggat ccacttccgg ccaggctatg agtatggccc tggcctggac gatctgcctg
     5221 aaaacctcta cggcccagat ggggctccct tctataacta cctaggcccc gaggacactg
     5281 cccctgagcc tcccttctcc aacccagcca gccagccggg agacaacaca cctgtccttg
     5341 agcctcctct gcagccctct gaacttcagc ctcactatct agccagccac tcagaacccc
     5401 tggcctcctt cgaaggcctt caggctgagg aatgtggcat cctgaatggc tgtgagaatg
     5461 gccgctgcgt gcgtgtgcgg gagggctaca cttgcgactg ctttgagggc ttccagctgg
     5521 atgcggccca catggcctgt gtggatgtga acgagtgtga agacttgaac gggcctgcag
     5581 cactctgtgc acacggtcac tgtgagaaca cagagggttc ctatcgctgc cactgttcgc
     5641 caggttacgt ggcagagcca ggccccccac actgtgcggc caaggagtag aagtgagggc
     5701 ttgtgtgggc agctatctgg aaaatggctt cagccatagg ccgtggactt aagattgctt
     5761 ccctagctgg gaagacatcg cgctgggaag atgacgtgat gacatcaggc ccgggctctg
     5821 gacccagttc tgccagcctt gccaaatttt tatttcttcc agcttaactc tgggtgtgag
     5881 cttccatcac tgcctctatg ccattgcttg gctcagacac cacaaatatc ttaatgcttc
     5941 agccactggc catgagacac gcccaccagt ctgtccccgg gaccacacta tagggcactc
     6001 atcaggctgt gctcttaggc tgtgcaaaca ttgcaggtac ccctttccag ttgcgagcca
     6061 cacatcatcc tagttgttct gtaatgggga cagtggctgc atccgcccag ggatggtctc
     6121 ttcacagtct gtggagcaag agagggtttg gggagtagct cctgtcacct ctcagaacca
     6181 acaccaccac tcggccaggc tggcatgagc tctttttgcc acatctccat ctcttagaca
     6241 gttctgcagc ccctcatctc agaggatgaa gactaatact aactcgctga gtggtaagaa
     6301 atacaaagga cgaggaacag tgagcctgag aaagtacggc aagagagtga tgtggaaaac
     6361 atggcgagct gatacgaaag gaggagccaa gagttaaaga aatctaaaaa cactgagttg
     6421 ctttgggcaa ataagtactt gaagcccctt agaggctctg tgtgcctgca gtgaggcttg
     6481 tcataagctc tggcgcccat atgcaaaggc ctttcttagg cacatctatc cctatgggga
     6541 aacccaaata gatgggctaa caatataaag gtctcctttt cagctggtct tggcagagga
     6601 ggccaggttt tcaaagttgt ttatctcctc cactcagaag cacttgccct tgttccctct
     6661 aagaaatgca cttccataca agctagctgg gggttcagga gcatggggga ataaaatgtt
     6721 catctgga
//
