LOCUS       NM_001331233            6342 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus latent transforming growth factor beta binding protein
            1 (Ltbp1), transcript variant 3, mRNA.
ACCESSION   NM_001331233 XM_017317494
VERSION     NM_001331233.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Kaneko,N., Hirai,K., Oshima,M., Yura,K., Hattori,M., Maeda,N. and
            Ohtaka-Maruyama,C.
  TITLE     ADAMTS2 promotes radial migration by activating TGF-beta signaling
            in the developing neocortex
  JOURNAL   EMBO Rep 25 (7), 3090-3115 (2024)
   PUBMED   38871984
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Neupane,S., Williamson,D.B., Roth,R.A., Halabi,C.M.,
            Haltiwanger,R.S. and Holdener,B.C.
  TITLE     Poglut2/3 double knockout in mice results in neonatal lethality
            with reduced levels of fibrillin in lung tissues
  JOURNAL   J Biol Chem 300 (7), 107445 (2024)
   PUBMED   38844137
REFERENCE   3  (bases 1 to 6342)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 6342)
  AUTHORS   Liu,B., Wang,Y., He,D., Han,G., Wang,H., Lin,Y., Zhang,T., Yi,C.
            and Li,H.
  TITLE     LTBP1 Gene Expression in the Cerebral Cortex and its
            Neuroprotective Mechanism in Mice with Postischemic Stroke Epilepsy
  JOURNAL   Curr Pharm Biotechnol 24 (2), 317-329 (2023)
   PUBMED   35676846
  REMARK    GeneRIF: LTBP1 Gene Expression in the Cerebral Cortex and its
            Neuroprotective Mechanism in Mice with Postischemic Stroke
            Epilepsy.
REFERENCE   5  (bases 1 to 6342)
  AUTHORS   Youlten,S.E., Kemp,J.P., Logan,J.G., Ghirardello,E.J., Sergio,C.M.,
            Dack,M.R.G., Guilfoyle,S.E., Leitch,V.D., Butterfield,N.C.,
            Komla-Ebri,D., Chai,R.C., Corr,A.P., Smith,J.T., Mohanty,S.T.,
            Morris,J.A., McDonald,M.M., Quinn,J.M.W., McGlade,A.R.,
            Bartonicek,N., Jansson,M., Hatzikotoulas,K., Irving,M.D.,
            Beleza-Meireles,A., Rivadeneira,F., Duncan,E., Richards,J.B.,
            Adams,D.J., Lelliott,C.J., Brink,R., Phan,T.G., Eisman,J.A.,
            Evans,D.M., Zeggini,E., Baldock,P.A., Bassett,J.H.D., Williams,G.R.
            and Croucher,P.I.
  TITLE     Osteocyte transcriptome mapping identifies a molecular landscape
            controlling skeletal homeostasis and susceptibility to skeletal
            disease
  JOURNAL   Nat Commun 12 (1), 2444 (2021)
   PUBMED   33953184
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6342)
  AUTHORS   Oklu,R. and Hesketh,R.
  TITLE     The latent transforming growth factor beta binding protein (LTBP)
            family
  JOURNAL   Biochem J 352 Pt 3 (Pt 3), 601-610 (2000)
   PUBMED   11104663
  REMARK    Review article
REFERENCE   7  (bases 1 to 6342)
  AUTHORS   Saharinen,J., Hyytiainen,M., Taipale,J. and Keski-Oja,J.
  TITLE     Latent transforming growth factor-beta binding proteins
            (LTBPs)--structural extracellular matrix proteins for targeting
            TGF-beta action
  JOURNAL   Cytokine Growth Factor Rev 10 (2), 99-117 (1999)
   PUBMED   10743502
  REMARK    Review article
REFERENCE   8  (bases 1 to 6342)
  AUTHORS   Nakajima,Y., Miyazono,K. and Nakamura,H.
  TITLE     Immunolocalization of latent transforming growth factor-beta
            binding protein-1 (LTBP1) during mouse development: possible roles
            in epithelial and mesenchymal cytodifferentiation
  JOURNAL   Cell Tissue Res 295 (2), 257-267 (1999)
   PUBMED   9931372
REFERENCE   9  (bases 1 to 6342)
  AUTHORS   Nakajima,Y., Miyazono,K., Kato,M., Takase,M., Yamagishi,T. and
            Nakamura,H.
  TITLE     Extracellular fibrillar structure of latent TGF beta binding
            protein-1: role in TGF beta-dependent endothelial-mesenchymal
            transformation during endocardial cushion tissue formation in mouse
            embryonic heart
  JOURNAL   J Cell Biol 136 (1), 193-204 (1997)
   PUBMED   9008713
REFERENCE   10 (bases 1 to 6342)
  AUTHORS   Yin,W., Smiley,E., Germiller,J., Mecham,R.P., Florer,J.B.,
            Wenstrup,R.J. and Bonadio,J.
  TITLE     Isolation of a novel latent transforming growth factor-beta binding
            protein gene (LTBP-3)
  JOURNAL   J Biol Chem 270 (17), 10147-10160 (1995)
   PUBMED   7730318
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154491.2, CT033758.4 and AC118018.20.
            
            On Sep 13, 2016 this sequence version replaced XM_017317494.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660821.77005.1,
                                           SRR1660817.212377.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-424               AC154491.2         24516-24939         c
            425-649             CT033758.4         82020-82244         c
            650-924             CT033758.4         80159-80433         c
            925-1027            CT033758.4         58404-58506         c
            1028-1099           CT033758.4         54985-55056         c
            1100-1222           CT033758.4         40790-40912         c
            1223-1390           CT033758.4         33366-33533         c
            1391-1618           CT033758.4         30777-31004         c
            1619-1641           CT033758.4         28426-28448         c
            1642-1741           CT033758.4         24973-25072         c
            1742-1837           CT033758.4         24382-24477         c
            1838-1963           CT033758.4         17260-17385         c
            1964-2089           CT033758.4         15984-16109         c
            2090-2212           CT033758.4         14522-14644         c
            2213-2332           CT033758.4         11057-11176         c
            2333-2455           AC118018.20        149653-149775       c
            2456-2578           AC118018.20        146833-146955       c
            2579-2701           AC118018.20        144806-144928       c
            2702-2824           AC118018.20        138838-138960       c
            2825-2950           AC118018.20        132609-132734       c
            2951-3076           AC118018.20        111062-111187       c
            3077-3220           AC118018.20        107101-107244       c
            3221-3400           AC118018.20        100462-100641       c
            3401-3487           AC118018.20        99133-99219         c
            3488-3616           AC118018.20        97293-97421         c
            3617-3757           AC118018.20        96453-96593         c
            3758-3928           AC118018.20        95422-95592         c
            3929-4051           AC118018.20        74736-74858         c
            4052-4201           AC118018.20        70352-70501         c
            4202-6342           AC118018.20        67419-69559         c
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 46.24 cM"
     gene            1..6342
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /note="latent transforming growth factor beta binding
                     protein 1"
                     /db_xref="GeneID:268977"
                     /db_xref="MGI:MGI:109151"
     exon            1..424
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     CDS             202..4383
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /note="isoform 3 precursor is encoded by transcript
                     variant 3; latent TGF beta binding protein; transforming
                     growth factor beta-1-binding protein 1; latent
                     transforming growth factor beta binding protein 1L"
                     /codon_start=1
                     /product="latent-transforming growth factor beta-binding
                     protein 1 isoform 3 precursor"
                     /protein_id="NP_001318162.1"
                     /db_xref="GeneID:268977"
                     /db_xref="MGI:MGI:109151"
                     /translation="MDTKLMCLLFFLCLPLLLVSNHTGRIKVVFTPSICKVTCTKGNC
                     QNSCQKGNTTTLISENGHAADTLTATNFRVVICHLPCMNGGQCSSRDKCQCPPNFTGK
                     LCQIPVLGASMPKLYQHAQQQGKALGSHVIHSTHTLPLTMTSQQGVKVKFPPNIVNIH
                     VKHPPEASVQIHQVSRIDSPGGQKVKEAQPGQSQVSYQGLPVQKTQTVHSTYSHQQLI
                     PHVYPVAAKTQLGRCFQETIGSQCGKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKQS
                     YHGYTQMMECLQGYKRVNNTFCQDINECQLQGVCPNGECLNTMGSYRCSCKMGFGPDP
                     TFSSCVPDPPVISEEKGPCYRLVSPGRHCMHPLSVHLTKQICCCSVGKAWGPHCEKCP
                     LPGTAAFKEICPGGMGYTVSGVHRRRPIHQHIGKEAVYVKPKNTQPVAKSTHPPPLPA
                     KEEPVEALTSSWEHGPRGAEPEVVTAPPEKEIPSLDQEKTRLEPGQPQLSPGVSTIHL
                     HPQFPVVEKTSPPVPVEVAPEASTSSASQVIAPTQVTEINECTVNPDICGAGHCINLP
                     VRYTCICYEGYKFSEQLRKCVDIDECAQVRHLCSQGRCENTEGSFLCVCPAGFMASEE
                     GTNCIDVDECLRPDMCRDGRCINTAGAFRCEYCDSGYRMSRRGYCEDIDECLKPSTCP
                     EEQCVNTPGSYQCVPCTEGFRGWNGQCLDVDECLQPKVCTNGSCTNLEGSYMCSCHRG
                     YSPTPDHRHCQDIDECQQGNLCMNGQCRNTDGSFRCTCGQGYQLSAAKDQCEDIDECE
                     HHHLCSHGQCRNTEGSFQCVCNQGYRASVLGDHCEDINECLEDSSVCQGGDCINTAGS
                     YDCTCPDGFQLNDNKGCQDINECAQPGLCGSHGECLNTQGSFHCVCEQGFSISADGRT
                     CEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGE
                     AFCENVEGSFLCVCADENQEYSPMTGQCRSRVTEDSGVDRQPREEKKECYYNLNDASL
                     CDNVLAPNVTKQECCCTSGAGWGDNCEIFPCPVQGTAEFTEMCPRGKGLVPAGESSYD
                     TGGENYKDADECLLFGEEICKNGYCLNTQPGYECYCKQGTYYDPVKLQCFDMDECQDP
                     NSCIDGQCVNTEGSYNCFCTHPMVLDASEKRCVQPTESNEQIEETDVYQDLCWEHLSE
                     EYVCSRPLVGKQTTYTECCCLYGEAWGMQCALCPMKDSDDYAQLCNIPVTGRRRPYGR
                     DALVDFSEQYGPETDPYFIQDRFLNSFEELQAEECGILNGCENGRCVRVQEGYTCDCF
                     DGYHLDMAKMTCVDVNECSELNNRMSLCKNAKCINTEGSYKCLCLPGYIPSDKPNYCT
                     PLNSALNLDKESDLE"
     exon            425..649
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            650..924
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1027
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1099
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1222
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1390
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1618
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1641
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1741
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1837
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1963
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2089
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2212
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2332
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2455
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2578
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            2579..2701
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..2824
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            2825..2950
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3076
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3220
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            3221..3400
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            3401..3487
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            3488..3616
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            3617..3757
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            3758..3928
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            3929..4051
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            4052..4201
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     exon            4202..6342
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5082..5087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /note="hexamer: ATTAAA"
     polyA_site      5109
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
     regulatory      5583..5588
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /note="hexamer: AATAAA"
     polyA_site      5605
                     /gene="Ltbp1"
                     /gene_synonym="9430031G15Rik; 9830146M04; b2b1000Clo;
                     Ltbp-1; Ltbp1L; TGF-beta1-BP-1; Tgfb"
                     /note="major polyA site"
ORIGIN      
        1 gttaagcttc ctactcctgt ttcagagaca ccactggata gagagagaga gcagcgagca
       61 agagcaggct tccctctgcc tgaaacctgt ccggctgtgg gctctctttc cctctctttc
      121 tttccctttc ctttttcttt ttccttttcc tttttttttt tttttttttt tttcggttga
      181 aaatttttcc aaggcaagtt catggatact aagctgatgt gtttgttgtt ctttctctgt
      241 ctgcctctac tcctagtgag taaccacact ggtcgcatca aggtggtctt tactccgagc
      301 atctgtaaag tgacctgcac caagggcaac tgtcagaaca gctgccagaa ggggaatacc
      361 accactctca ttagtgagaa cggccatgca gccgacaccc tgacagccac gaacttccga
      421 gtggttattt gccatcttcc gtgtatgaat ggtggccagt gcagttcaag ggacaaatgt
      481 cagtgccctc caaatttcac aggaaagctt tgccagatcc ctgtccttgg tgccagtatg
      541 cccaaactct accagcacgc ccagcagcag ggcaaggcat tggggagtca cgtcatccat
      601 tccacacata ccttgcctct aaccatgact agccagcaag gggtcaaagt gaaattcccc
      661 cccaacatag tcaatatcca tgtgaaacat cctcccgagg cttctgtcca gatacaccag
      721 gtttccagga ttgacagccc aggaggccag aaggtgaagg aagcccaacc aggccagtcc
      781 caagtctctt accaaggact tccggtccag aagacccaga cagtccattc tacgtactcc
      841 caccagcagc ttattcccca tgtgtatccc gtggctgcta agacacaact tggccgatgc
      901 ttccaggaaa ccattggatc acagtgtggc aaagcgctcc ctggcctttc caagcaagag
      961 gactgctgtg ggacagtggg gacgtcctgg ggctttaaca aatgccagaa gtgccccaag
     1021 aaacaatcct atcatggata tactcaaatg atggaatgcc tacaaggata taaacgggtt
     1081 aacaacacct tttgtcaaga tattaatgaa tgtcagctac aaggtgtatg ccctaatggt
     1141 gagtgtttga ataccatggg cagctacaga tgctcctgca aaatgggatt cgggcctgac
     1201 cctacctttt caagttgtgt tccggatccc cctgtgatct cagaagagaa agggccctgt
     1261 taccgccttg tcagtcctgg aaggcattgt atgcaccctc tctctgttca tctcaccaag
     1321 cagatctgct gttgtagtgt gggcaaggcc tggggcccac attgtgagaa atgtcccctc
     1381 ccaggcacag ctgcttttaa ggaaatctgt cctggtggaa tgggttatac ggtttctggc
     1441 gttcatagac gcaggccaat ccatcagcat ataggtaaag aagctgtata tgtcaagcca
     1501 aagaacactc aacctgttgc taaaagtact catcctccac ctctcccagc caaggaagag
     1561 cctgtggagg ccctgacctc ctcgtgggaa cacgggccga gaggggcgga gcccgaagtg
     1621 gtaacagcac ctcctgagaa ggaaatacct tcactggatc aagagaaaac cagacttgaa
     1681 cctgggcaac cccagctctc cccaggggtt tcaactattc atctgcaccc acagtttcca
     1741 gtggttgaaa agacatcccc tcctgtgcct gtggaagtag ctcctgaagc ttccacatcc
     1801 agtgccagcc aagtgattgc tcctacgcaa gtaacagaaa tcaacgaatg tactgtgaac
     1861 ccggacattt gcggagcagg acactgcatc aacctgccgg tgcggtatac ctgcatatgc
     1921 tacgagggct acaagttcag tgaacagctg aggaaatgtg tcgacattga tgaatgtgct
     1981 caggtccggc acctctgctc ccagggtcgc tgtgagaaca cagagggaag tttcctgtgt
     2041 gtctgcccgg cagggtttat ggccagtgag gagggtacta actgtataga tgtggatgag
     2101 tgcctgaggc ctgatatgtg tagggatggc cgctgcatca acaccgctgg ggccttccgg
     2161 tgcgagtact gtgacagtgg gtaccggatg tcacggagag gctactgtga ggatattgat
     2221 gagtgtctga agccaagcac ttgtcccgag gaacagtgtg tgaacacccc gggctcttac
     2281 cagtgtgtgc cctgcacaga ggggttccgc ggctggaatg gacaatgcct tgatgtggac
     2341 gagtgcctgc agccaaaggt ctgtacgaat ggttcctgca ccaacctgga agggtcctac
     2401 atgtgttcct gccacagggg ctacagcccc acaccagacc atagacactg tcaagatatt
     2461 gatgaatgcc aacaagggaa tctctgcatg aatgggcagt gcagaaatac cgatggctcc
     2521 ttccggtgta cctgtggaca gggctatcag ctgtcagcag ctaaagacca atgtgaagat
     2581 attgatgaat gcgagcacca tcacctctgc tctcacgggc agtgcaggaa cacagagggc
     2641 tccttccagt gtgtgtgcaa ccagggttac agagcatctg tacttggaga ccactgtgag
     2701 gatatcaatg aatgcttgga ggacagcagt gtctgccagg gaggtgactg catcaatact
     2761 gcggggtcct atgattgcac atgcccggat ggattccagc tgaacgacaa taagggctgt
     2821 caagacatta atgaatgtgc acagccagga ctctgtggat ctcatgggga gtgtctaaac
     2881 acacaaggct ccttccactg tgtctgtgaa caggggttct ccatctctgc cgatggccgt
     2941 acatgcgaag atattgatga gtgtgttaac aacactgtgt gtgacagtca cggcttctgt
     3001 gacaacacag ccggctcttt ccgctgcctc tgttatcagg gctttcaagc cccacaggat
     3061 gggcaagggt gtgtggatgt gaacgagtgt gaactgctca gtggtgtatg tggggaagcc
     3121 ttctgtgaaa acgtggaagg atccttcctg tgtgtgtgtg ccgatgagaa ccaggagtac
     3181 agccccatga ctgggcagtg tcgctctcgg gtcacggaag attcaggtgt ggatcgtcag
     3241 cctagagaag agaagaagga gtgctattat aacctcaatg atgctagtct ctgtgataat
     3301 gtcctggccc ccaacgtcac caaacaagag tgctgctgta cgtcaggtgc cggctgggga
     3361 gacaactgtg agatcttccc ttgccccgtc cagggaactg ctgagttcac ggaaatgtgc
     3421 cctagaggga aaggtttggt cccggctgga gaatcctctt acgacactgg tggcgagaac
     3481 tacaaagatg ctgatgaatg cttgctgttt ggagaggaaa tctgtaaaaa tggttactgt
     3541 ttgaacactc aacctgggta tgaatgctac tgtaagcaag ggacatacta tgatcctgtc
     3601 aaattacagt gctttgatat ggatgaatgc caagacccta acagttgcat tgacggccag
     3661 tgtgtgaaca cagagggctc ctacaactgc ttttgcaccc acccaatggt cctggatgcc
     3721 tctgagaaga gatgtgtgca accaactgaa tcaaatgaac aaatagaaga gaccgatgtc
     3781 tatcaagatc tgtgctggga gcatctgagt gaggagtacg tgtgtagccg tcctctcgta
     3841 ggcaagcaga cgacgtacac agaatgctgc tgtttgtacg gggaggcttg gggcatgcag
     3901 tgtgctctgt gccccatgaa ggactcagat gactatgccc agctgtgcaa tatccctgtg
     3961 actgggcgtc ggcggccata tggacgggat gcattggtgg acttcagtga acagtatggc
     4021 ccagaaacag acccttactt catccaggat cgctttctaa atagctttga ggagctacag
     4081 gctgaggagt gtggcatcct caatggttgt gaaaacggcc gctgtgtgag agtccaggaa
     4141 ggatacactt gcgattgctt tgatggatat cacctggata tggccaagat gacctgtgtc
     4201 gatgtaaatg aatgcagcga gctgaataat cggatgtctc tctgcaagaa cgccaagtgc
     4261 attaacacag aaggctccta caaatgcttg tgtctgccag gctacatacc atccgacaag
     4321 cccaactact gtacacccct gaacagcgct ttgaatttag acaaagaaag tgacctggag
     4381 tgaaggagaa gaagctgcgt aacctatgcc catatactct gcactgtgta aagggaaagg
     4441 gagagatgta tacttgagac actgcaccta acccagaatg tggcaaagac agagacaact
     4501 tggagttgag tgaaccccca aagacagtga gcggatggag ctggaacctg acaggtgcaa
     4561 cagaccaaat ggacatttct tcaaaaaccc agtatatata gtctgttcat atgtaaaatt
     4621 cagtggaaaa gaggcaggac atggctgtca ttttaaacag aggttgtatt attatcttgt
     4681 tttgattttt ttttttacta ttgcttaaat ttggctttta aatattagtg gaaatgtttt
     4741 atgtgatctt tctttttgct gtgcagatcc tcagctgccg tttttttttt cctccaatga
     4801 taatctcaag tgcaaacatc tttgataaaa tattgttaaa ctgtattata agtaatatga
     4861 cacagggttg tgctgtccct tggtctggag acttttaaaa aaacaagttg tctgtcctgt
     4921 ggagcaggca gtaattgtta aacttttgga ggggatggag aaacatcttt atttcaatgt
     4981 attaccagat tgcctgtcaa ttggatccct tacctggatg gaagtggaag agcttcatag
     5041 agtttattaa tattgtgatg tgtaaagtca accgagaaaa aattaaaaac ctgaacatcc
     5101 ccccccaaac atttaccatt gtatgttata agaaaataaa ctcaccattt ttgtagaaaa
     5161 aaaaaatttg agtgtggaag aagaagttgt ccccttcctc ataacagctc ccccatctct
     5221 gatcttttaa ccactagtcc tccatcacgc taggcttctg taagttagga ctggttagct
     5281 actgttacag gttgctaatt tccatcttag ttccaggctg aagtgctgca tccccgtgct
     5341 ttagaatgcg tggattctct ttggagacaa gatctctaag gaggaaatga aagttacagg
     5401 agaccagtgg agtaaactgt aacccaagac tatgggtgtc cttagatgac agggaagtaa
     5461 ggacttgtgc agagaaaagg tcatttgaag agggctggaa ggcgaccatc ttgcaaaaga
     5521 gtgaggcctc agagggaagc caactctgcc acagagtttg acagagtcca gaattatgag
     5581 aaaataaact tctgttgtca aacaacctgt ctatgatact ttcttatggc ctttccagac
     5641 aaccaataca gacttgtata acaggaataa tgggcaaaca aggcctttca aagtcccggt
     5701 ctctgatctc atatggacga cccctagcaa tgatagctgc ttcactcact gtgaaactta
     5761 ctgtgcctca gcaaaccccc atgggaaatc ccccagtgaa cacttccttg aggggccttt
     5821 gccttctaat gcctgtagca ggcaaagtga taacaactta aagatataga tcttctaacc
     5881 tatggactct gtcataatga agggaagtac tgtctcagag aagtctgcca agtcacttgg
     5941 ccccaaagag gacgatgatc ttggattgcc tggctattat tactatgtaa tcaccagggg
     6001 tgtccttgga acattaaaag tggcaagaca gaaagaaaga agttctgaaa atgtgacatg
     6061 caagaggtca agtacagcac taaagtgctt cttgtcacca ctgactggta aaggacatga
     6121 acccaagatg tggacagcta gtagaagccg ggcaagacga ggagctggct cttcatagac
     6181 aatctagaaa taagaatagc actattatac ctgatacctt gttttcagtc tagtgagcct
     6241 gtgtgaacct ttcacctatt gagctgtaag ataactgggt attgtttgat gtgactgagt
     6301 ttgtaacaat agaaaattaa agctgcttag aagcttaaag tc
//
