LOCUS       NM_008517               2066 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus leukotriene A4 hydrolase (Lta4h), transcript variant
            1, mRNA.
ACCESSION   NM_008517 XM_914122
VERSION     NM_008517.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wu,X., Sun,A.R., Crawford,R., Xiao,Y., Wang,Y., Prasadam,I. and
            Mao,X.
  TITLE     Inhibition of Leukotriene A4 Hydrolase Suppressed Cartilage
            Degradation and Synovial Inflammation in a Mouse Model of
            Experimental Osteoarthritis
  JOURNAL   Cartilage 15 (2), 184-194 (2024)
   PUBMED   37086004
  REMARK    GeneRIF: Inhibition of Leukotriene A4 Hydrolase Suppressed
            Cartilage Degradation and Synovial Inflammation in a Mouse Model of
            Experimental Osteoarthritis.
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2066)
  AUTHORS   Adams,J.M., Rege,S.V., Liu,A.T., Vu,N.V., Raina,S., Kirsher,D.Y.,
            Nguyen,A.L., Harish,R., Szoke,B., Leone,D.P., Czirr,E.,
            Braithwaite,S. and Kerrisk Campbell,M.
  TITLE     Leukotriene A4 hydrolase inhibition improves age-related cognitive
            decline via modulation of synaptic function
  JOURNAL   Sci Adv 9 (46), eadf8764 (2023)
   PUBMED   37976357
  REMARK    GeneRIF: Leukotriene A4 hydrolase inhibition improves age-related
            cognitive decline via modulation of synaptic function.
REFERENCE   4  (bases 1 to 2066)
  AUTHORS   Shioda,R., Jo-Watanabe,A., Okuno,T., Saeki,K., Nakayama,M.,
            Suzuki,Y. and Yokomizo,T.
  TITLE     The leukotriene B4 /BLT1-dependent neutrophil accumulation
            exacerbates immune complex-mediated glomerulonephritis
  JOURNAL   FASEB J 37 (2), e22789 (2023)
   PUBMED   36692419
REFERENCE   5  (bases 1 to 2066)
  AUTHORS   Hopke,A., Lin,T., Scherer,A.K., Shay,A.E., Timmer,K.D.,
            Wilson-Mifsud,B., Mansour,M.K., Serhan,C.N., Irimia,D. and
            Hurley,B.P.
  TITLE     Transcellular biosynthesis of leukotriene B4 orchestrates
            neutrophil swarming to fungi
  JOURNAL   iScience 25 (10), 105226 (2022)
   PUBMED   36267914
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2066)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
REFERENCE   7  (bases 1 to 2066)
  AUTHORS   Byrum,R.S., Goulet,J.L., Snouwaert,J.N., Griffiths,R.J. and
            Koller,B.H.
  TITLE     Determination of the contribution of cysteinyl leukotrienes and
            leukotriene B4 in acute inflammatory responses using
            5-lipoxygenase- and leukotriene A4 hydrolase-deficient mice
  JOURNAL   J Immunol 163 (12), 6810-6819 (1999)
   PUBMED   10586081
REFERENCE   8  (bases 1 to 2066)
  AUTHORS   Wetterholm,A., Medina,J.F., Radmark,O., Shapiro,R.,
            Haeggstrom,J.Z., Vallee,B.L. and Samuelsson,B.
  TITLE     Recombinant mouse leukotriene A4 hydrolase: a zinc metalloenzyme
            with dual enzymatic activities
  JOURNAL   Biochim Biophys Acta 1080 (2), 96-102 (1991)
   PUBMED   1932096
REFERENCE   9  (bases 1 to 2066)
  AUTHORS   Medina,J.F., Wetterholm,A., Radmark,O., Shapiro,R.,
            Haeggstrom,J.Z., Vallee,B.L. and Samuelsson,B.
  TITLE     Leukotriene A4 hydrolase: determination of the three zinc-binding
            ligands by site-directed mutagenesis and zinc analysis
  JOURNAL   Proc Natl Acad Sci U S A 88 (17), 7620-7624 (1991)
   PUBMED   1881903
REFERENCE   10 (bases 1 to 2066)
  AUTHORS   Medina,J.F., Radmark,O., Funk,C.D. and Haeggstrom,J.Z.
  TITLE     Molecular cloning and expression of mouse leukotriene A4 hydrolase
            cDNA
  JOURNAL   Biochem Biophys Res Commun 176 (3), 1516-1524 (1991)
   PUBMED   1710117
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY793553.1, AK134931.1,
            AW121450.1 and BY458968.1.
            
            On Oct 27, 2006 this sequence version replaced NM_008517.1.
            
            Summary: The protein encoded by this gene is an enzyme that
            contains both hydrolase and aminopeptidase activities. The
            hydrolase activity is used in the final step of the biosynthesis of
            leukotriene B4, a proinflammatory mediator. The aminopeptidase
            activity has been shown to degrade proline-glycine-proline (PGP), a
            neutrophil chemoattractant and biomarker for chronic obstructive
            pulmonary disease (COPD). Two transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134931.1, M63848.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BY793553.1         2-16
            16-1793             AK134931.1         2-1779
            1794-2045           AW121450.1         17-268              c
            2046-2066           BY458968.1         384-404
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 48.42 cM"
     gene            1..2066
                     /gene="Lta4h"
                     /note="leukotriene A4 hydrolase"
                     /db_xref="GeneID:16993"
                     /db_xref="MGI:MGI:96836"
     exon            1..273
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..1950
                     /gene="Lta4h"
                     /EC_number="3.3.2.6"
                     /EC_number="3.4.11.4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leukotriene A-4 hydrolase; LTA-4 hydrolase; LTA4
                     hydrodase; tripeptide aminopeptidase LTA4H"
                     /codon_start=1
                     /product="leukotriene A-4 hydrolase isoform 1"
                     /protein_id="NP_032543.2"
                     /db_xref="CCDS:CCDS24125.1"
                     /db_xref="GeneID:16993"
                     /db_xref="MGI:MGI:96836"
                     /translation="MPEVADTCSLASPASVCRTQHLHLRCSVDFARRTLTGTAALTVQ
                     SQEENLRSLTLDTKDLTIEKVVINGQEVKYTLGESQGYKGSPMEISLPIALSKNQEIV
                     IEISFETSPKSSALQWLTPEQTSGKQHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAE
                     VSVPKELVALMSAIRDGEAPDPEDPSRKIYRFNQRVPIPCYLIALVVGALESRQIGPR
                     TLVWSEKEQVEKSANEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCL
                     TFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLF
                     GEKFRHFHALGGWGELQNTIKTFGESHPFTKLVVDLKDVDPDVAYSSIPYEKGFALLF
                     YLEQLLGGPEVFLGFLKAYVKKFSYQSVTTDDWKSFLYSHFKDKVDLLNQVDWNTWLY
                     APGLPPVKPNYDVTLTNACIALSQRWVTAKEEDLSSFSIADLKDLSSHQLNEFLAQVL
                     QKAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEEAIPLALKMATEQGRMK
                     FTRPLFKDLAAFDKSHDQAVHTYQEHKASMHPVTAMLVGRDLKVD"
     exon            274..404
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            405..525
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            526..594
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            595..699
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            700..752
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            753..825
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            826..966
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            967..990
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1061
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1173
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1318
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1422
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1493
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1548
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1644
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1727
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1832
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     exon            1833..2066
                     /gene="Lta4h"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2020..2025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lta4h"
                     /note="hexamer: AATACA"
     polyA_site      2045
                     /gene="Lta4h"
                     /note="major polyA site"
ORIGIN      
        1 ggggtgcacc gcgccatcta ccctctgagc ttccctccgc tctcgggctc gcggtgaaag
       61 tctggcggta gagtgtctgg gtgctggctg acgtgcggcg gatctcccac aaccatgccc
      121 gaggtcgcgg atacttgctc cttggcttct ccagcctcgg tctgccgaac ccagcacctc
      181 catcttcgct gcagcgtcga cttcgctcgc cggacgctga ccgggacggc cgcgctcacg
      241 gtccagtcac aggaggagaa tctgcgcagc ctgactttgg acacaaaaga cctcacaata
      301 gaaaaagtgg tgatcaatgg acaagaagtc aaatacaccc ttggagaaag tcaaggttac
      361 aaagggtcac cgatggaaat ctctcttccc atcgccctga gcaaaaatca agagatcgtt
      421 atagaaattt cctttgagac atctcccaaa tcctctgcgc ttcagtggct cactcctgaa
      481 cagacgtcag ggaagcagca tccgtatctc ttcagtcagt gccaggccat ccactgcagg
      541 gccatcctcc cttgccaaga cactccttcc gtgaaattaa cgtacaccgc agaggtgtct
      601 gtcccgaaag aactggtggc tctcatgagt gccatccgtg atggagaagc ccctgaccca
      661 gaagacccca gcaggaagat ttacagattc aaccagaggg ttcccatacc atgctacctg
      721 attgctttgg ttgttggagc tttagaaagc agacaaattg gcccaagaac tctggtgtgg
      781 tccgagaaag agcaggtgga gaaatctgct aatgaatttt ctgagaccga atccatgctt
      841 aaaattgcag aagatctggg ggggccctat gtttgggggc agtacgacct cttggttctg
      901 cccccatcct tcccgtacgg tggcatggag aacccttgtc tcacgttcgt aacgcctacg
      961 ctactggcag gtgacaagtc actctccaac gttattgcac atgaaatatc tcatagctgg
     1021 acaggaaacc tagtgactaa caagacgtgg gatcactttt ggttaaacga aggacatact
     1081 gtctacttag agcgccacat ttgtggacgg ttgtttgggg aaaagttcag acatttccat
     1141 gctttgggag gatggggaga gctacagaat acgataaaaa catttgggga gtcccacccc
     1201 ttcaccaagc tcgtggttga tctgaaggac gtagaccccg atgtagccta ttcctccatc
     1261 ccctatgaga agggctttgc gctactcttc taccttgaac agcttcttgg gggaccagag
     1321 gtgtttctag gattcttaaa ggcttatgtt aagaagtttt cctaccaaag tgtaacgact
     1381 gatgactgga agagcttcct atattcccac tttaaagaca aggtggatct tctcaatcaa
     1441 gttgattgga acacctggct ctacgctccg ggcctgcctc ctgtcaagcc caattacgac
     1501 gtgactctga caaatgcttg cattgcctta agtcaaagat gggttactgc caaagaggaa
     1561 gatttaagct cattcagcat cgcagacctg aaggatctct cgtcccatca gctgaatgag
     1621 ttcctggcac aggtgcttca gaaggcacct cttcccttgg ggcacataaa gcgaatgcaa
     1681 gaggtgtaca acttcaatgc cattaacaat tctgaaatac gattcagatg gttacggctc
     1741 tgcattcaat cgaaatggga ggaagcaatt cctttggccc taaagatggc aactgaacaa
     1801 ggaaggatga aattcacacg tcctctattc aaggatctcg ctgccttcga caaatcccat
     1861 gatcaagctg tccacaccta ccaagagcat aaagccagca tgcatcccgt gactgcgatg
     1921 ctggtgggga gagatctgaa agtggattaa ggaactgatt gttgctgatt tcagaaaatt
     1981 cctttttttt aaaaaaaaaa aaaattgaac ctattaagaa atacaaaatt ttagctgtca
     2041 ttgtatttta aagtgtcatt tttggt
//
