LOCUS       NM_146006               6339 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus lanosterol synthase (Lss), transcript variant 1, mRNA.
ACCESSION   NM_146006
VERSION     NM_146006.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Zhao,M., Mei,T., Shang,B., Zou,B., Lian,Q., Xu,W., Wu,K., Lai,Y.,
            Liu,C., Wei,L., Zhu,J., Zhang,K., Liu,Y. and Zhao,L.
  TITLE     Defect of LSS Disrupts Lens Development in Cataractogenesis
  JOURNAL   Front Cell Dev Biol 9, 788422 (2021)
   PUBMED   34926465
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6339)
  AUTHORS   Wedel,M., Frob,F., Elsesser,O., Wittmann,M.T., Lie,D.C., Reis,A.
            and Wegner,M.
  TITLE     Transcription factor Tcf4 is the preferred heterodimerization
            partner for Olig2 in oligodendrocytes and required for
            differentiation
  JOURNAL   Nucleic Acids Res 48 (9), 4839-4857 (2020)
   PUBMED   32266943
REFERENCE   4  (bases 1 to 6339)
  AUTHORS   Wada,Y., Kikuchi,A., Kaga,A., Shimizu,N., Ito,J., Onuma,R.,
            Fujishima,F., Totsune,E., Sato,R., Niihori,T., Shirota,M.,
            Funayama,R., Sato,K., Nakazawa,T., Nakayama,K., Aoki,Y., Aiba,S.,
            Nakagawa,K. and Kure,S.
  TITLE     Metabolic and pathologic profiles of human LSS deficiency
            recapitulated in mice
  JOURNAL   PLoS Genet 16 (2), e1008628 (2020)
   PUBMED   32101538
  REMARK    GeneRIF: Study identified two patients with novel biallelic LSS
            mutations who exhibited congenital hypotrichosis and midline
            anomalies but did not have cataracts. Epidermis-specific Lss
            knockout mice showed neonatal lethality due to dehydration,
            indicating that LSS could be involved in skin barrier integrity.
            Knockout of Lss in the epidermis caused hypotrichosis in adult
            mice. Lens-specific Lss knockout mice had cataracts.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6339)
  AUTHORS   Kim,M., Wende,H., Walcher,J., Kuhnemund,J., Cheret,C., Kempa,S.,
            McShane,E., Selbach,M., Lewin,G.R. and Birchmeier,C.
  TITLE     Maf links Neuregulin1 signaling to cholesterol synthesis in
            myelinating Schwann cells
  JOURNAL   Genes Dev 32 (9-10), 645-657 (2018)
   PUBMED   29748249
  REMARK    Erratum:[Genes Dev. 2018 Aug 1;32(15-16):1101. doi:
            10.1101/gad.318477.118.. PMID: 30068704]
REFERENCE   6  (bases 1 to 6339)
  AUTHORS   Nagashima,S., Yagyu,H., Tozawa,R., Tazoe,F., Takahashi,M.,
            Kitamine,T., Yamamuro,D., Sakai,K., Sekiya,M., Okazaki,H.,
            Osuga,J., Honda,A. and Ishibashi,S.
  TITLE     Plasma cholesterol-lowering and transient liver dysfunction in mice
            lacking squalene synthase in the liver
  JOURNAL   J Lipid Res 56 (5), 998-1005 (2015)
   PUBMED   25755092
  REMARK    GeneRIF: liver-specific ablation of squalene synthase inhibits
            hepatic cholesterol biosynthesis and induces hypolipidemia without
            increasing significant mortality.
REFERENCE   7  (bases 1 to 6339)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 6339)
  AUTHORS   Sacchetti,P., Sousa,K.M., Hall,A.C., Liste,I., Steffensen,K.R.,
            Theofilopoulos,S., Parish,C.L., Hazenberg,C., Richter,L.A.,
            Hovatta,O., Gustafsson,J.A. and Arenas,E.
  TITLE     Liver X receptors and oxysterols promote ventral midbrain
            neurogenesis in vivo and in human embryonic stem cells
  JOURNAL   Cell Stem Cell 5 (4), 409-419 (2009)
   PUBMED   19796621
REFERENCE   9  (bases 1 to 6339)
  AUTHORS   Dang,H., Liu,Y., Pang,W., Li,C., Wang,N., Shyy,J.Y. and Zhu,Y.
  TITLE     Suppression of 2,3-oxidosqualene cyclase by high fat diet
            contributes to liver X receptor-alpha-mediated improvement of
            hepatic lipid profile
  JOURNAL   J Biol Chem 284 (10), 6218-6226 (2009)
   PUBMED   19119143
  REMARK    GeneRIF: Suppression of 2,3-oxidosqualene cyclase by high fat diet
            contributes to liver X receptor-alpha-mediated improvement of
            hepatic lipid profile.
REFERENCE   10 (bases 1 to 6339)
  AUTHORS   Wiltshire,T., Pletcher,M., Cole,S.E., Villanueva,M., Birren,B.,
            Lehoczky,J., Dewar,K. and Reeves,R.H.
  TITLE     Perfect conserved linkage across the entire mouse chromosome 10
            region homologous to human chromosome 21
  JOURNAL   Genome Res 9 (12), 1214-1222 (1999)
   PUBMED   10613844
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ128932.1, AK141216.1,
            AK031271.1, AK078023.1, AK147680.1, AK014742.1 and BY474863.1.
            
            On Feb 11, 2015 this sequence version replaced NM_146006.1.
            
            Summary: This gene encodes an enzyme that catalyzes the conversion
            of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a
            member of the terpene cyclase/mutase family and catalyzes the first
            step in the biosynthesis of cholesterol, steroid hormones, and
            vitamin D. [provided by RefSeq, Feb 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK031271.1, AK154520.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 CJ128932.1         2-3
            3-15                AK141216.1         3-15
            16-705              AK031271.1         4-693
            706-706             AK078023.1         687-687
            707-2097            AK031271.1         695-2085
            2098-2098           AK147680.1         2762-2762
            2099-4669           AK031271.1         2087-4657
            4670-5237           AK147680.1         5334-5901
            5238-5238           AK014742.1         172-172
            5239-6328           AK147680.1         5903-6992
            6329-6339           BY474863.1         506-516
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.1 cM"
     gene            1..6339
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /note="lanosterol synthase"
                     /db_xref="GeneID:16987"
                     /db_xref="MGI:MGI:1336155"
     exon            1..70
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     CDS             57..2258
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /EC_number="5.4.99.7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     oxidosqualene--lanosterol cyclase;
                     2,3-epoxysqualene--lanosterol cyclase"
                     /codon_start=1
                     /product="lanosterol synthase isoform 1"
                     /protein_id="NP_666118.1"
                     /db_xref="CCDS:CCDS23948.1"
                     /db_xref="GeneID:16987"
                     /db_xref="MGI:MGI:1336155"
                     /translation="MTEGTCLRRRGGPYKTEPATDLTRWRLQNELGRQRWTYYQAEDD
                     PGREQTGLEAHSLGLDTRSYFTDLPKAQTAHEGALNGVTFYAKLQAEDGHWAGDYGGP
                     LFLLPGLLITCHISHISLPAGYREEMVRYLRSVQLPDGGWGLHIEDKSTVFGTALNYV
                     ALRILGIGPDDPDLVRARNVLHKKGGAVAIPSWGKFWLAVLNVYSWEGLNTLFPEMWL
                     FPEWVPAHPSTLWCHCRQVYLPMSYCYATRLSASEDPLVQSLRQELYVQDYASIDWPA
                     QRNNVSPDEMYTPHSWLLHVVYGLLNLYERFHSTSLRKWAVQMLYEHIAADDCFTKCI
                     SIGPISKTINMLVRWSVDGPSSPAFQEHVSRIKDYLWLGLDGMKMQGTNGSQIWDTSF
                     AIQALLEAGAHHRPEFLPCLQKAHEFLRLSQVPENCPDYQKYYRHMRKGGFSFSTLDC
                     GWIVADCTAEGLKAVLLLQNQCPSITEHIPRERLCDAVDVLLSLRNADGGFATYEKKR
                     GGYLLELLNPSEVFGDIMIDYTYVECTSAVMQALKHFHEHFPDYRAAEVRETLNQGLD
                     FCRRKQRADGSWEGSWGVCFTYGTWFGLEAFACMGHTYQDGAACAEVAQACNFLLSQQ
                     MADGGWGEDFESCEQRRYVQSARSQVHSTCWALMGLMAVRHPDITAQERGIRCLLGKQ
                     LPNGDWPQENISGVFNKSCAISYTSYRNIFPIWALGRFSNLYPDNTLAGHI"
     exon            71..239
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            240..378
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            379..487
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            488..609
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            610..706
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            707..842
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            843..951
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1070
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1168
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1196
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1253
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1325
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..1376
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1526
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1623
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1729
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1795
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1876
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..2047
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2126
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     exon            2127..6329
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3093..3098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /note="hexamer: AATAAA"
     regulatory      3106..3111
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /note="hexamer: AATAAA"
     polyA_site      3121
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /note="major polyA site"
     polyA_site      3124
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /note="major polyA site"
     regulatory      6307..6312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /note="hexamer: AATAAA"
     polyA_site      6329
                     /gene="Lss"
                     /gene_synonym="2810025N20Rik; D10Ertd116e; Osc"
                     /note="major polyA site"
ORIGIN      
        1 gtgcactagg ctgtcaggta aagggctggc aagcggtggc agagagtagt gctgtcatga
       61 ctgagggcac gtgtctgcgg cgtcgtgggg gaccctataa aactgagccc gccaccgatc
      121 tcacccgctg gcggctccag aatgagttgg gtcggcagag atggacttat tatcaagcgg
      181 aggacgaccc tggtcgagag cagaccgggc tagaggccca ctctttggga ctggacacaa
      241 gaagttattt cacggactta ccaaaagcgc aaacagccca tgagggggcc ctgaatggag
      301 taacctttta tgccaagctg caggctgagg atggacactg ggctggtgat tatggtggtc
      361 cgctcttcct cttgccaggt ctcctgatta cctgtcacat atcacacatc tctctgccag
      421 ctggatacag agaggagatg gtgcggtacc tgcggtcagt gcagctccct gatggcggct
      481 ggggcttgca catcgaggac aagtccacag tgtttgggac tgccctgaac tatgtggctc
      541 tcagaatcct gggtattggg cctgatgatc ctgaccttgt gcgcgctcgg aacgtccttc
      601 acaaaaaagg tggtgcggtg gccatccctt cctgggggaa gttctggctg gctgtcctga
      661 atgtttatag ctgggaagga ctcaacaccc tattccctga gatgtggctg tttcctgaat
      721 gggtaccagc gcacccctcc actctctggt gtcactgccg gcaggtctat ctgcccatga
      781 gttactgcta cgccactcgg ctaagtgcct cagaggaccc actggttcag agcctccgcc
      841 aggaactcta tgtgcaggat tacgccagca tcgattggcc agcgcagagg aacaacgtgt
      901 cccccgatga gatgtacacg ccacacagct ggctgctgca tgtggtgtat ggactcctca
      961 acctgtatga acgtttccat agcaccagcc tgcggaagtg ggccgtccag atgctgtatg
     1021 aacacattgc agctgatgac tgtttcacta agtgcatcag catcggcccg atctcaaaga
     1081 ccatcaacat gcttgttcgt tggtcagtgg atggaccatc ctccccggcc ttccaggagc
     1141 acgtttctcg gatcaaagat tacctttggc tgggccttga tggcatgaaa atgcagggca
     1201 ccaatggatc acagatctgg gacacttcat ttgctatcca agcactgtta gaggcaggtg
     1261 cacaccacag acctgagttt ctgccctgcc tgcagaaggc tcacgagttc ctgcggcttt
     1321 cacaggttcc agaaaactgt cctgactatc agaagtacta tcgccatatg cgcaagggtg
     1381 gtttctcctt cagcacactg gactgtggct ggatcgttgc tgactgcaca gccgagggtt
     1441 tgaaggctgt gctgctcctg cagaatcagt gtccgtccat cactgagcat atcccccgag
     1501 agcggctgtg cgatgctgtg gatgtgttac tgagcttgag gaatgctgat ggcgggttcg
     1561 ctacctatga gaagaagcgt gggggttact tgctggagct gctgaacccc tcagaggtct
     1621 ttggagacat catgattgac tacacgtatg tagagtgtac ctccgctgtg atgcaggcac
     1681 tgaagcattt ccatgaacac ttcccagact acagggcagc agaggtcagg gagaccctca
     1741 atcaaggcct ggacttctgc cgaaggaagc agagagccga tggctcctgg gagggctcct
     1801 ggggtgtttg tttcacctac ggcacctggt ttggcttgga agcgttcgct tgcatggggc
     1861 atacctacca agatggggct gcttgtgcag aagtagctca ggcctgcaac ttcctcctgt
     1921 cccagcagat ggcggatgga ggctggggag aggactttga atcatgtgaa cagcggcggt
     1981 atgtgcagag tgctaggtcc caggtccata gcacctgctg ggccctgatg ggtttgatgg
     2041 ctgtcaggca tcccgacatc actgctcagg agagagggat cagatgtctg ctagggaagc
     2101 agttacccaa tggagactgg cctcaggaga acatctctgg ggtcttcaac aagtcctgtg
     2161 ccatcagcta cacaagttac agaaacatct tccccatctg ggccctgggc cgcttctcca
     2221 acctgtatcc tgacaatacc ctcgccggcc acatttgaac acgtgtgtgt ctgctgcacc
     2281 tggccagcat catcaccatc caggtcctgg agagccccgt gtcttggctg ggtgataaga
     2341 gccttcttag ccctgcttag gcctcgttct ctatttctgt agacaggagg ctggggatag
     2401 ggtcagtgtt ggtgtcttta gacacttgtc agtgtgcgct taaagttctt ggactagctt
     2461 cagtgagaaa gggaagaaga tgagccttga gatccagaga ggcgcagtgg atggactcgg
     2521 aagctcctgg gtattcgttc tccccgaggg agagaggcta tagagcttct gtgatcaatg
     2581 acttccctgt gagggagcag ctggcttctt cactgcttcc cttgttgatt gtggagtggg
     2641 cacttgagca tcttgtgttt gctgtgctga gggagttaga gcagccatat ggtgcagggg
     2701 tgctcagaga aaggctcaat ggcaggtgag gcaggtccct tactcattgt gccctgagca
     2761 gtgtctggtg tccctagaaa cttgctaccc atgactacat cactatatga aatgccagtt
     2821 attcagaaag gaaatacaaa ctggctttgt accagaaatg ctactaaaag tcatttgaat
     2881 gatcaccgac tcccttacta aacagaccct gggcgtagta aagaacagcg gctgctctct
     2941 cagaggacct gggtttgatt cctggcaccc acctggtggc taacagcatg taactccagt
     3001 tctcagggaa cccaatgtca tcttctggcc tctgtgggca ctgcacacat ggtacataga
     3061 catacataca ctcataacat ctatacacat aaaataaatc ctgaaaataa aatattaaaa
     3121 acaaccacct gccttcctga gacatttcat cctggagtgt ctatgctgag tctgtgtggc
     3181 aaggagccag ttgtagcatc caattctgcc cccagattat atgcttatac aattgcattg
     3241 gtttcttaca agatcagcat cctgatgtcc aggcttctca gtccttgttg agatagagac
     3301 gcacacgtgg gggagccatt agggtagaca gtggttgtaa cggttcctgc catgggacag
     3361 tgctggagca aggacagcta atcatatcca gctcaccact tagacacacc aaggcagcca
     3421 ggaaaatgcc catgacaccc aaagatcgtt atcagcccat atgccagctg tgctttgtga
     3481 gtccaagtga gggccagatg tctgaggcaa tcctttttgg gtgtgagctc ttaagttcct
     3541 gattgtaact gtgtgtctgg ccagagcagg cagagttcag ggtggaaact atgtgtctcc
     3601 ttgaagttac tgcctttctc tctggccatg gtgagcgggg tccagctgga tgccccttta
     3661 gttaactaac tctcggcatc tgtagtgctt tctgcactgg tatctccaat cgctccctga
     3721 cccatagtct tccctccgac aagcagaaag ctgtgtctga tccccattcc cctgtgcagg
     3781 accctctagg gcagcctttg gccatggtgc actttgtgcc ctctgcctgt tgcccagctg
     3841 ccaccatcga caccttgtgc tgtgactcag ggctttcctg ccttagtggc ccgtgctgtt
     3901 cacagcactg acttctaacc tctgccagga gtagaactgg tgaactcagc tagatctgct
     3961 cctagcagca gcaaccatcc cttggaattc ccggttacga accagatcac tgtacattcc
     4021 cttaacaaac agactgctgg cgagacgtgc tttaggctgt agcaggtcat gctgtggagt
     4081 tctccggaca tgctggttta ccagccccag tttagggagt tctccgggct ccgcgggggg
     4141 tgggggttgg tggggggaca ctcaccagat aactgtccag aagaagttct taaggatgct
     4201 taaaatgaaa gttggagcct gtcaggaata attcggaatg ctgatggctt ttcctgtgga
     4261 gtcacagcac atttccttgg ttaactatga tgtgaacctc aacacggttt tgagggattc
     4321 tttagtaaga aatgttgaca tttctgcccc cccctccccc acgctgaagc agttttcctg
     4381 tgtggtactc actatgacat gatcttcact acgtagtcca ggatggcctc aagcttgagg
     4441 gtagtcctgc ctccaccttc agagcgctgg gatacaggcg tgtgccacca tcccctgctg
     4501 aagggcttga tggttaagtc tctctgacaa agtatttttt ccttttaaaa tatgacttac
     4561 tgctaagtag aattgaattt actaagatgt ttttatttaa ataaattttt gctgaatgct
     4621 gttttctcat gatctgtttt aacaaataca actgtatggt gatatatagt taatcagatt
     4681 gatttatttt taattagtta atttattaat tggtttgaga cagggtctca ctgtatagct
     4741 ctggccatcc tgggactcaa tatgtatata gagcaggcta gcttccagtt cacagaagtc
     4801 cacctgcctc ttcctccaag tgctgggatt aaaggcatgc actacaatat atgactttca
     4861 acttgactta tttccatctt aactttattt tatctctgta ttttatgcag atactactac
     4921 tatctgtttt tctaaagctt cagatgtttt tattcaaagg tttacaaaag gaataaaaca
     4981 gaaaaagcta acaatgtgat caaatgtaca gttccaaaat ggcctttggg gctttaacag
     5041 tgagtcctag gaaggaaact gcagtcatcc tgacctcgta gataagtctc ctggcaaggg
     5101 ggtagtaccc gtagaatgct aaaaattagc tcttgatcat gtacttcact atgaaaatct
     5161 tttttaaaaa atctgtataa aaggtcttta taaatcaatt tttaaataat ggacatccac
     5221 tatgtgatat gtaactgact tttgttttat gtatagaggt tatatttccc aaatctgacc
     5281 cagatgagta tggtactcag ttcagacata gctagcagct gattttcccc ttctctcctg
     5341 actgaattat caacttcttg tctgaactaa tgcaaaaaca atccagcagt gagagacctg
     5401 gaatgataaa tgtgctaggt acacgatgga gaggcgttca gtgtggactc ccacaaagca
     5461 gagtgtctag gatgtgtggg cccagtcaca tcaacgcttc ttaaaaggaa acaagaacaa
     5521 aaccacattt ctcttatgag tataattttc attaggatct acacttccca gtgcagcaag
     5581 gatgcttgaa acgatgaaat gccaatgata cccatcatca aagacaagca atcaaggcca
     5641 ggcttaatgg cacatgcctt taatcccagc acttgagagg cagcggcaac agcagacgaa
     5701 tttctgagtt cagggccagc ctggtctact gcagagtgag ttccaggaca gccaggaata
     5761 catagagaaa ccctgtctca aaaaaaaacc caaagccaac caaccaaaca aaaagacaag
     5821 taatcaaatt taagaactaa agtcctcgaa agaaagaact gcttagccct agctctggag
     5881 ctagggggac ttgcttgaca gaaagagggc cagcaacctg tcctagcaca aaagaaaaac
     5941 agcctttgag gtagatagct gatcatacac agccaatcac gcgcacagtt ttggaaaaca
     6001 acattgcttt taagaaattg ttttgggttt ggattcatgg ctcagtactt aagagcacta
     6061 gcttaattcc cagcacccac agggcagctc acaatctact gtaactcaaa ttctaggaga
     6121 tctgacatcc tcacaccaat gcacattaaa taaaacagtt aaaaagtatt aaaaattgcc
     6181 ttaataaaaa agtaacagtg aagtatctca tgttctgaag tagaaataat taggaatgag
     6241 caatgtttca aaattgtttt ctttttcttt tcacttgaac actacaaaca ttatgcagca
     6301 atgacaaata aagtgtggca ctgcttggaa aaaaaaaaa
//
