LOCUS       NM_027891               2201 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus leucine-rich repeats and WD repeat domain containing 1
            (Lrwd1), mRNA.
ACCESSION   NM_027891
VERSION     NM_027891.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Kang,T.Z.E., Wan,Y.C.E., Zhang,Z. and Chan,K.M.
  TITLE     Lrwd1 impacts cell proliferation and the silencing of repetitive
            DNA elements
  JOURNAL   Genesis 60 (4-5), e23475 (2022)
   PUBMED   35451548
  REMARK    GeneRIF: Lrwd1 impacts cell proliferation and the silencing of
            repetitive DNA elements.
REFERENCE   3  (bases 1 to 2201)
  AUTHORS   Scovell,J.M., Bournat,J.C., Szafran,A.T., Solis,M., Moore,J.,
            Rivera,A., Chen,C.H., Zhang,J., Wilken,N., Seth,A. and Jorgez,C.J.
  TITLE     PRSS50 is a testis protease responsible for proper sperm tail
            formation and function
  JOURNAL   Development 148 (8) (2021)
   PUBMED   33913480
REFERENCE   4  (bases 1 to 2201)
  AUTHORS   Chan,K.M. and Zhang,Z.
  TITLE     Leucine-rich repeat and WD repeat-containing protein 1 is recruited
            to pericentric heterochromatin by trimethylated lysine 9 of histone
            H3 and maintains heterochromatin silencing
  JOURNAL   J Biol Chem 287 (18), 15024-15033 (2012)
   PUBMED   22427655
  REMARK    GeneRIF: Leucine-rich repeat and WD repeat-containing protein 1 is
            recruited to pericentric heterochromatin by trimethylated lysine 9
            of histone H3 and maintains heterochromatin silencing
REFERENCE   5  (bases 1 to 2201)
  AUTHORS   Teng,Y.N., Liao,M.H., Lin,Y.B., Kuo,P.L. and Kuo,T.Y.
  TITLE     Expression of lrwd1 in mouse testis and its centrosomal
            localization
  JOURNAL   Int J Androl 33 (6), 832-840 (2010)
   PUBMED   20180869
  REMARK    GeneRIF: immunostaining and immunoprecipitation showed
            colocalization and interaction between lrwd1 and gamma-tubulin
            respectively, implicating lrwd1 as a candidate centrosomal protein.
            These results suggest that lrwd1 may play an important role in
            spermatogenesis.
REFERENCE   6  (bases 1 to 2201)
  AUTHORS   Shen,Z., Sathyan,K.M., Geng,Y., Zheng,R., Chakraborty,A.,
            Freeman,B., Wang,F., Prasanth,K.V. and Prasanth,S.G.
  TITLE     A WD-repeat protein stabilizes ORC binding to chromatin
  JOURNAL   Mol Cell 40 (1), 99-111 (2010)
   PUBMED   20932478
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY142519.1, AK084948.1 and AA620153.1.
            
            On Feb 5, 2008 this sequence version replaced NM_027891.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC048397.1, AK153976.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 BY142519.1         2-4
            4-2173              AK084948.1         4-2173
            2174-2201           AA620153.1         316-343
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 75.8 cM"
     gene            1..2201
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /note="leucine-rich repeats and WD repeat domain
                     containing 1"
                     /db_xref="GeneID:71735"
                     /db_xref="MGI:MGI:1918985"
     exon            1..203
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..2070
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /note="ORC-associated protein; origin recognition
                     complex-associated protein"
                     /codon_start=1
                     /product="leucine-rich repeat and WD repeat-containing
                     protein 1"
                     /protein_id="NP_082167.2"
                     /db_xref="CCDS:CCDS51666.1"
                     /db_xref="GeneID:71735"
                     /db_xref="MGI:MGI:1918985"
                     /translation="MAPLTPQLLLQRGRPKTDKLGKIQSLNLSGLQLLSEHLDPNLLG
                     RLKKLRELDLSNNLLETLPANLGLSHLRILRCTNNQLGDVTALHQFPELEELNLEGNP
                     FLTVSDNLKVSFLLPKLRKVNGKDTASTCSQVENLDRELMDRVTAHWQKFIATVSPEE
                     ETDKVRADFMRSAVRDVCYGPESLIEFTQWRVRMIAEELVASGGAQVQDAKVPVEHPQ
                     AAGASKFRAREVASKRPGKDPVTLPPSKRVRALPPAQAEGSPMGADGGQAALHLEPLH
                     FLQCHSRNNSPKDLETQLWACAFEPAREEGHSRATSQTVATCGGEAVCVIDCQTGLVL
                     HKYKVPGEEFFSVAWTALTVVTQAGHKKRWNMLAAAGLRGMVRLLHVRAGFCCSVIRA
                     HKKAIATLCFSPSHETHLFTASYDKRIILWDIGVPNQDYKFQASQLLTLNCGSVPLRL
                     CPVATCPDDFLLAGCEGGCYCWDVRLDQPQKQRVCEVNFIFSEDSKVSGQRVDGLAFV
                     NEDVVASKGSGQGTIYLWSWSQTWAGRGRQSVLPVVILVRLQWSPTNLAYFSLSTCPG
                     KNLVLCGDEEGSVWIYDVEHLLKEPPQATTLQPPTQILKWPQPTALGQPVTKTMINTV
                     VANAAFTYLTALTDSNIVSIWRRC"
     exon            204..438
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            439..555
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            556..696
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            697..801
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            802..924
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1039
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1149
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1357
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1430
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1571
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1663
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1819
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1929
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2179
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2155..2160
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /note="hexamer: ATTAAA"
     polyA_site      2179
                     /gene="Lrwd1"
                     /gene_synonym="1200011O22Rik; Orca"
                     /note="major polyA site"
ORIGIN      
        1 ttccttccgg tcgcaacgga ctcgcaaagg gtttgttgtg cgccacctcc tgggctcccc
       61 ggcagcgggc cttagcgacc ggacttcgtg gaggcgcggg ttcggttgag ccctcggcca
      121 cccatggctc ctctcacgcc gcaactgctc ctgcaacgtg ggcgacccaa gacggataag
      181 ctggggaaga ttcagagttt gaatctgtcg gggctgcagc tgctctcaga gcacttggat
      241 cccaatctcc tggggcgcct gaagaagctg agggaacttg acctctctaa caacttgctg
      301 gagacgctgc ctgctaacct gggcctgtcc catctgcgca tccttcgctg taccaacaac
      361 cagctcgggg atgtcactgc cttgcaccag ttcccagaac ttgaggagct caatctggaa
      421 ggcaacccct tcctgacggt cagtgacaac ctcaaagtct cctttctcct gcccaagctt
      481 cgtaaggtga atggcaagga cacggcctcc acctgctcac aggtggagaa tctagatcgg
      541 gagctgatgg acagggtcac agctcactgg cagaagttca tagccactgt gagccctgag
      601 gaggaaactg acaaggtgcg ggctgatttc atgaggtctg cagtcaggga tgtgtgttac
      661 ggccctgagt cgctcatcga gttcacgcag tggcgggtgc ggatgatcgc cgaggaactg
      721 gtggcctcag gtggggccca ggtgcaagac gctaaggtcc ccgtggagca tccccaggct
      781 gcaggtgcct ccaagttcag ggccagagag gtggcttcaa aacggccagg taaagaccca
      841 gtcacccttc ctcccagcaa gcgagtgcgt gctctcccac ctgctcaggc tgagggcagt
      901 cctatgggtg ctgatggagg gcaggctgcc ctacacctgg aacccctgca cttcctgcag
      961 tgtcatagca gaaacaacag cccaaaggac ctggagaccc agctgtgggc ctgtgccttt
     1021 gagccggccc gggaggaggg gcactcgagg gccacatccc agaccgtggc cacatgtggt
     1081 ggagaggctg tttgcgtgat agactgccag acaggcctcg tactccataa gtacaaggta
     1141 ccaggcgagg agttcttctc agtggcctgg acagccctca cggtggtcac ccaggcaggc
     1201 cacaagaagc gctggaacat gctagcagct gcaggcctcc gcggcatggt ccggctgctg
     1261 catgttcgcg caggcttctg ctgcagcgtc atccgggccc ataagaaggc cattgccacc
     1321 ctctgcttca gccccagcca tgagactcac ctcttcactg cctcctacga caaacggatc
     1381 atcctctggg acattggggt gcccaaccag gattacaagt tccaggctag ccagctcctc
     1441 acgctcaact gtggttctgt ccccttgcgc ctctgtcctg ttgccacctg cccagatgac
     1501 ttcctgctgg ctggctgtga gggtggctgc tactgctggg acgtgcggct agaccagccc
     1561 cagaagcaaa gggtgtgtga ggtgaacttc atcttctctg aggactccaa ggtgtcagga
     1621 caaagagtcg atgggctggc atttgtgaat gaagatgtgg tggcctccaa agggagtgga
     1681 cagggcacca tctacctgtg gagctggagc cagacatggg caggccgggg cagacagtct
     1741 gtgttgcccg tagtcatcct ggtccggctg cagtggtcac ccaccaacct ggcctacttt
     1801 tcactcagca cctgtcccgg caaaaacctc gtgctgtgtg gggacgagga aggcagtgtg
     1861 tggatctatg atgttgaaca cctcctgaaa gagcctccac aggcaacaac tctgcagccc
     1921 ccaacacaga tcctgaagtg gcctcagcca acagcattgg gccaaccagt gaccaagacc
     1981 atgataaaca cagtcgtggc caatgcagcc ttcacctacc tcactgccct gacagactcc
     2041 aacatcgtgt ccatctggag gagatgctag tctgcctgca caaggcagtg tgtcaggaca
     2101 tggcttgtta gcttgggcta atgggagagg gctttgtacc agtgagtatt ttttattaaa
     2161 ttctctattg tagagtggaa aaaaaaaaaa aaaaaaaaaa a
//
