LOCUS       NM_199302               4062 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Mus musculus leucine rich repeat and sterile alpha motif containing
            1 (Lrsam1), transcript variant 2, mRNA.
ACCESSION   NM_199302 XM_006497969 XM_149118
VERSION     NM_199302.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Cui,Q., Bi,H., Lv,Z., Wu,Q., Hua,J., Gu,B., Huo,C., Tang,M.,
            Chen,Y., Chen,C., Chen,S., Zhang,X., Wu,Z., Lao,Z., Sheng,N.,
            Shen,C., Zhang,Y., Wu,Z.Y., Jin,Z., Yang,P., Liu,H., Li,J. and
            Bai,G.
  TITLE     Diverse CMT2 neuropathies are linked to aberrant G3BP interactions
            in stress granules
  JOURNAL   Cell 186 (4), 803-820 (2023)
   PUBMED   36738734
REFERENCE   3  (bases 1 to 4062)
  AUTHORS   Moiseev,D., Wazir,Z., Liu,D., Li,J. and Hu,B.
  TITLE     C698R mutation in Lrsam1 gene impairs nerve regeneration in a CMT2P
            mouse model
  JOURNAL   Sci Rep 12 (1), 12160 (2022)
   PUBMED   35842440
  REMARK    GeneRIF: C698R mutation in Lrsam1 gene impairs nerve regeneration
            in a CMT2P mouse model.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4062)
  AUTHORS   Hu,B., Cao,L., Wang,X.Y. and Li,L.
  TITLE     Downregulation of microRNA-431-5p promotes enteric neural crest
            cell proliferation via targeting LRSAM1 in Hirschsprung's disease
  JOURNAL   Dev Growth Differ 61 (4), 294-302 (2019)
   PUBMED   31037734
  REMARK    GeneRIF: downregulation of miR-431-5p promoted proliferation of
            enteric neural crest cells via targeting LRSAM1, which provides an
            innovative and candidate target for treatment of Hirschsprung's
            disease
REFERENCE   5  (bases 1 to 4062)
  AUTHORS   Yang,J.C., Wu,S.C., Rau,C.S., Chen,Y.C., Lu,T.H., Wu,Y.C.,
            Tzeng,S.L., Wu,C.J. and Hsieh,C.H.
  TITLE     TLR4/NF-kappaB-responsive microRNAs and their potential target
            genes: a mouse model of skeletal muscle ischemia-reperfusion injury
  JOURNAL   Biomed Res Int 2015, 410721 (2015)
   PUBMED   25692136
  REMARK    GeneRIF: TLR4/NF-kappaB-responsive microRNAs and their potential
            target genes: a mouse model of skeletal muscle ischemia-reperfusion
            injury.
REFERENCE   6  (bases 1 to 4062)
  AUTHORS   Bogdanik,L.P., Sleigh,J.N., Tian,C., Samuels,M.E., Bedard,K.,
            Seburn,K.L. and Burgess,R.W.
  TITLE     Loss of the E3 ubiquitin ligase LRSAM1 sensitizes peripheral axons
            to degeneration in a mouse model of Charcot-Marie-Tooth disease
  JOURNAL   Dis Model Mech 6 (3), 780-792 (2013)
   PUBMED   23519028
  REMARK    GeneRIF: Lrsam1 mutant mice are more sensitive to challenge with
            acrylamide, a neurotoxic agent that causes axon degeneration,
            indicating that the axons in the mutant mice are indeed
            compromised.
REFERENCE   7  (bases 1 to 4062)
  AUTHORS   Amit,I., Yakir,L., Katz,M., Zwang,Y., Marmor,M.D., Citri,A.,
            Shtiegman,K., Alroy,I., Tuvia,S., Reiss,Y., Roubini,E., Cohen,M.,
            Wides,R., Bacharach,E., Schubert,U. and Yarden,Y.
  TITLE     Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor
            endocytosis and retrovirus budding
  JOURNAL   Genes Dev 18 (14), 1737-1752 (2004)
   PUBMED   15256501
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731852.9 and AL929154.6.
            
            On or before Apr 2, 2020 this sequence version replaced
            XM_006497969.4, NM_199302.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1 and encodes the longest isoform (a). Variants 1-3 all
            encode the same isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253012.9725231.1,
                                           SRR13422598.515571.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AL731852.9         168156-168235
            81-292              AL731852.9         168542-168753
            293-389             AL731852.9         170184-170280
            390-446             AL731852.9         171583-171639
            447-491             AL731852.9         172504-172548
            492-569             AL731852.9         173245-173322
            570-638             AL731852.9         174548-174616
            639-723             AL731852.9         175511-175595
            724-845             AL731852.9         176312-176433
            846-936             AL731852.9         178614-178704
            937-1067            AL731852.9         181876-182006
            1068-1097           AL731852.9         183676-183705
            1098-1220           AL731852.9         183824-183946
            1221-1360           AL731852.9         184216-184355
            1361-1405           AL731852.9         185048-185092
            1406-1476           AL731852.9         187924-187994
            1477-1576           AL731852.9         189457-189556
            1577-1664           AL731852.9         190913-191000
            1665-1739           AL731852.9         193287-193361
            1740-1820           AL731852.9         194549-194629
            1821-1916           AL731852.9         195887-195982
            1917-2015           AL929154.6         18992-19090         c
            2016-2147           AL929154.6         16475-16606         c
            2148-2229           AL929154.6         15553-15634         c
            2230-2375           AL929154.6         14886-15031         c
            2376-4062           AL929154.6         12116-13802         c
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.09 cM"
     gene            1..4062
                     /gene="Lrsam1"
                     /note="leucine rich repeat and sterile alpha motif
                     containing 1"
                     /db_xref="GeneID:227738"
                     /db_xref="MGI:MGI:2684789"
     exon            1..80
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            81..292
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            293..389
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     CDS             318..2501
                     /gene="Lrsam1"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 2; E3
                     ubiquitin-protein ligase LRSAM1; tsg101-associated ligase;
                     leucine-rich repeat and sterile alpha motif-containing
                     protein 1; RING-type E3 ubiquitin transferase LRSAM1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase LRSAM1 isoform a"
                     /protein_id="NP_955006.1"
                     /db_xref="CCDS:CCDS15935.1"
                     /db_xref="GeneID:227738"
                     /db_xref="MGI:MGI:2684789"
                     /translation="MPLFFRKRKPSEEARKRLEYQMCLAKEAGADDILDISKCELSEI
                     PFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLVTIKVLDLHENQLTALPDDMGQ
                     LTVLQVLNVERNQLTHLPRSIGNLLQLQTLNVKDNKLKELPDTLGELRSLRTLDISEN
                     EIQRLPQMLAHVRTLETLSLNALAMVYPPPEVCGAGTAAVQQFLCKESGLDYYPPSQY
                     LLPVLEQDGAENTQDSPDGPASRFSREEAEWQNRFSDYEKRKEQKMLEKLEFERRLDL
                     GQREHAELLQQSHSHKDEILQTVKQEQTRLEQDLSERQRCLDAERQQLQEQLKQTEQS
                     IASRIQRLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETENLRQREIAAAMQQM
                     LTESCKSRLIQMAYESQRQSLAQQACSSMAEMDKRFQQILSWQQMDQNKAISQILQES
                     VMQKAAFEALQVKKDLMHRQIRNQIRLIETELLQLTQLELKRKSLDTETLQEMVSEQR
                     WALSNLLQQLLKEKKQREEELHGILAELEAKSETKQENYWLIQYQRLLNQKPLSLKLQ
                     EEGMERRLVALLVELSAEHYLPLFAHHRISLDMLSRMSPGDLAKVGVSEAGLQHEILR
                     RAQDLLAVPRVQPELKPLENEVLGALEPPTAPRELQESVRPSAPPAELDMPTSECVVC
                     LEREAQMVFLTCGHVCCCQQCCQPLRTCPLCRQEISQRLRIYHSS"
     exon            390..446
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            447..491
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            492..569
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            570..638
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            639..723
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            724..845
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            846..936
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1067
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1097
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1220
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1360
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1405
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1476
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1576
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..1664
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1739
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1740..1820
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1916
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2015
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..2147
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2229
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            2230..2375
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..4062
                     /gene="Lrsam1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4032..4037
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrsam1"
                     /note="hexamer: AATAAA"
     polyA_site      4062
                     /gene="Lrsam1"
                     /note="major polyA site"
ORIGIN      
        1 agtctctggg cgacaccctg gtcgccctgt ttcttatggg caggcgtctg cggcagagga
       61 ctgcagagct tggtctgtgg aatctcgaga ctttgtcatc ctgagttgcg tgtctttctg
      121 aaatttaaag tttcggtgct cacttctatg tttgaaggag accggacacc agctcagctt
      181 ttgggggcca atggtttgta tctgtggcca agtcttcgga gtgactggcc taccttgagg
      241 tccacccaag aatcggaaca tcggtggagg acctccccat ccacagagcc agggtccaga
      301 agagctcaca ccggaggatg cccctcttct ttcggaagcg gaaacccagt gaggaggctc
      361 gaaaacgcct ggagtaccag atgtgtctgg caaaagaagc tggggcagat gacattctcg
      421 acatctctaa atgtgagctc tctgagattc catttggggc ttttgcaacg tgcaaagttc
      481 tacagaaaaa ggtgttgatt gtccatacaa accacctcac ctccctgctt cccaagtcct
      541 gcagcctctt gagccttgtc accatcaagg ttctggatct ccatgagaac cagctgacag
      601 cccttcctga tgacatgggg cagctgacag tcctgcaggt attgaatgtg gaaagaaatc
      661 aactcacgca tctccctcgc tctattggga acctgctgca gctccagacg ctcaatgtaa
      721 aagacaacaa gctgaaggag cttcctgaca ccctggggga gctgcggagc ctgcggacac
      781 tcgacattag tgagaacgag attcagagac ttccccagat gctggcgcac gtgcggaccc
      841 tggagacgct gagcctcaac gccttggcaa tggtctaccc cccaccagag gtgtgtggcg
      901 ctggcactgc ggccgtgcag cagttcctct gcaaagagtc aggactggac tattacccac
      961 cttctcagta cctgctgcca gtcctggagc aagatggagc agagaacacc caagacagcc
     1021 ccgatggacc cgcaagccga ttctccaggg aggaggctga atggcagaat cggttctccg
     1081 actacgagaa gcggaaggag cagaagatgc tggagaagct ggagttcgag cggcgcctgg
     1141 accttgggca gcgggagcac gctgagctac tgcagcagag ccacagccac aaggacgaga
     1201 tcctgcagac ggtcaagcag gagcagacac ggctagagca ggacctgagc gagcgccagc
     1261 gctgtctgga tgcagagcgg cagcagctgc aggagcagct caagcagacg gagcagagca
     1321 tcgccagccg cattcagaga ctcctgcagg acaaccagag gcaaaagaag agttctgaga
     1381 ttctgaaatc gctggagaat gagagaataa gaatggagca gttgatgtcc atcacccagg
     1441 aggagacaga gaacctcagg cagcgtgaga tcgccgccgc catgcagcag atgctgacgg
     1501 agagctgtaa gagccggctc atccagatgg cctatgagtc tcagaggcag agcctggcgc
     1561 agcaggcctg ttccagcatg gctgaaatgg acaagcggtt ccagcagatt ctgtcttggc
     1621 agcagatgga tcagaacaaa gccatcagcc agatccttca ggagagtgta atgcagaagg
     1681 ctgccttcga ggctctccag gtgaagaagg acctgatgca tcggcagatc aggaaccaga
     1741 ttaggctaat agaaactgag ttactgcagc tgacacagct ggagttaaag aggaagtccc
     1801 tggacacaga gacgcttcag gagatggtct cagagcagcg ctgggcactc agcaacctgc
     1861 tccagcagct cctgaaagag aagaagcagc gggaagagga actccatggc atcctggcgg
     1921 aattagaggc caagagcgaa acgaagcagg aaaattactg gctcatccag taccaacggc
     1981 ttttaaacca gaagcctttg tccttgaaac tgcaggaaga aggcatggag cgacggctgg
     2041 tggccctgct ggtggagctt tctgcagagc actacctgcc cctcttcgcc caccaccgca
     2101 tctcactgga catgctgagc cgaatgagcc ccggagatct ggctaaggtg ggcgtctcag
     2161 aagcaggcct gcaacatgag atcctacgga gagcccagga cctgctagct gtgcctaggg
     2221 tccagccaga gttgaaacca ctcgagaatg aggtccttgg tgccctcgag ccccccacag
     2281 ctccccggga gctccaagag tcagtgcgac cgtccgcccc cccagctgaa ctggacatgc
     2341 cgacctcaga gtgtgttgtg tgcctggaac gtgaagccca gatggtcttc ctcacctgtg
     2401 gccacgtctg ctgctgtcag cagtgctgcc agcctctgcg cacctgccca ctgtgccgcc
     2461 aggagatctc ccagcgcctc cggatctacc acagcagctg aaggcacagc acctaccagc
     2521 cctggccctt ccttggtacc atctccacca ctgctggact cccacctacc ctcattcccc
     2581 agactagggc aagactgcca taggcctttg tcacccacca cctgccaccc cgacccccac
     2641 tgctctgagc actaggcagg caggtggaat gttcctggcc tgtgacatag ctattctgaa
     2701 cagtgaacaa aagacagggc aaatctgctg gtggtggagc cttggggcag ggggccaagg
     2761 tccagcgtag ggaggtggcc ctgcaggact caggaatcca tgttagtagc caagcctcag
     2821 cgctacaccc agctgtctgc ccctcctgga ggtgtccata tctgtggaat gtatcagcca
     2881 tcccgtccta aggtgcacca gtgtctctgg gaaggggtgg gttgtgcaag actccccagc
     2941 acccttttgc ttctttctga acacccaccc tcccatatct gcatccttag cctacacagg
     3001 caggttgatt caaaacagga gcaccagtaa atctgactcc ttgcagagtc acccctaggt
     3061 ccatgagaac ttgggagggg taggagtagg cagagtccgg ggagagggca gctcttctgg
     3121 ggaaaagggc gagagctgct gcccctgggt cagagggaag gcaacagaaa cattgcccca
     3181 tggagagtta ggcaacaccc tcttgaaaga gggcgagggg aacagagcca gagatggggg
     3241 tctcattatt tatttataag tagcctgtgc tcatgggaca ggttgtgttc ctgcaatgat
     3301 tatcctaggt gagggggctg ctgaggcaac tggaaggcat gttaactttc agaggggcag
     3361 cccaccctca ccagctgtag tgaagggttc catgtgacat ggcaccctac tgccctcccc
     3421 agtatagtgt atatggccta cagcatgtgc tcaacaaata ttactacttg actggctctg
     3481 ggggaactag acattagcac ctagctcagg cctggaggcc accctcatgg tggacacatg
     3541 ggactgatga gccatcagaa ggctagtcac ctgaaagctc tatggcctct ccccttcttt
     3601 ctgagcctca gtttctccct ctaaaatagg ggattgtaag aatgagttag agaaagaggc
     3661 ctcagtacat tagttgtcat ttggaagtct ggctccccag atctcacaga gccaggccag
     3721 tacatgtaat caaagtactg aggaagctga agccggcaga ccctgggact caccagtcag
     3781 ccaagctaac cgagttagtg agttccaggc taatgagacc tcatctcaaa acacgaggcg
     3841 gaatgatccg agatcctttg gcttccacat gcacatgtga atttgtacct gtacacacgt
     3901 gcaggaacac actcagatca ggggcctggt agctcatatg atgagcccaa gagatctggg
     3961 atgaatggat ccctagatac ggtaggccca gatctccccc accccagcag cacatgagga
     4021 aatgagcttt aaataaagcg tttattgatg agcagaagct ta
//
