LOCUS       NM_025730               8231 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus leucine-rich repeat kinase 2 (Lrrk2), mRNA.
ACCESSION   NM_025730 XM_155643
VERSION     NM_025730.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Yang,K., Zhou,Y., Cui,J., Tang,W., Chen,Y. and Chen,X.
  TITLE     LRRK2 G2019S enhances immune response pathways and aggravates
            asthma in mouse models
  JOURNAL   Biochem Biophys Res Commun 734, 150593 (2024)
   PUBMED   39217812
  REMARK    GeneRIF: LRRK2 G2019S enhances immune response pathways and
            aggravates asthma in mouse models.
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Lin,Y.E., Jaimon,E., Tonelli,F. and Pfeffer,S.R.
  TITLE     Pathogenic LRRK2 mutations cause loss of primary cilia and
            Neurturin in striatal parvalbumin interneurons
  JOURNAL   Life Sci Alliance 8 (1), e202402922 (2024)
   PUBMED   39537338
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8231)
  AUTHORS   Chang,E.E., Liu,H., Choi,Z.Y., Malki,Y., Zhang,S.X., Pang,S.Y.,
            Kung,M.H., Ramsden,D.B., Ho,S.L. and Ho,P.W.
  TITLE     Loss of mitochondrial Ca2+ response and CaMKII/ERK activation by
            LRRK2R1441G mutation correlate with impaired depolarization-induced
            mitophagy
  JOURNAL   Cell Commun Signal 22 (1), 485 (2024)
   PUBMED   39390438
  REMARK    GeneRIF: Loss of mitochondrial Ca[2+] response and CaMKII/ERK
            activation by LRRK2[R1441G] mutation correlate with impaired
            depolarization-induced mitophagy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8231)
  AUTHORS   Heaton,G.R., Li,X., Zhou,X., Zhang,Y., Vu,D.T., Oeller,M.,
            Karayel,O., Hoang,Q.Q., Kars,M.E., Wang,M., Tarassishin,L.,
            Mann,M., Peter,I. and Yue,Z.
  TITLE     Targeting Specific Kinase Substrates Rescues Increased Colitis
            Severity Induced by the Crohn's Disease-Linked LRRK2-N2081D Variant
  JOURNAL   bioRxiv (2024)
   PUBMED   39282291
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8231)
  AUTHORS   Gupta,S., Tielemans,A., Guevara,C.A., Huntley,G.W. and Benson,D.L.
  TITLE     Parkinson's-linked LRRK2-G2019S derails AMPAR trafficking,
            mobility, and composition in striatum with cell-type and subunit
            specificity
  JOURNAL   Proc Natl Acad Sci U S A 121 (28), e2317833121 (2024)
   PUBMED   38968112
  REMARK    GeneRIF: Parkinson's-linked LRRK2-G2019S derails AMPAR trafficking,
            mobility, and composition in striatum with cell-type and subunit
            specificity.
REFERENCE   6  (bases 1 to 8231)
  AUTHORS   Galter,D., Westerlund,M., Carmine,A., Lindqvist,E., Sydow,O. and
            Olson,L.
  TITLE     LRRK2 expression linked to dopamine-innervated areas
  JOURNAL   Ann Neurol 59 (4), 714-719 (2006)
   PUBMED   16532471
REFERENCE   7  (bases 1 to 8231)
  AUTHORS   Simon-Sanchez,J., Herranz-Perez,V., Olucha-Bordonau,F. and
            Perez-Tur,J.
  TITLE     LRRK2 is expressed in areas affected by Parkinson's disease in the
            adult mouse brain
  JOURNAL   Eur J Neurosci 23 (3), 659-666 (2006)
   PUBMED   16487147
REFERENCE   8  (bases 1 to 8231)
  AUTHORS   Melrose,H., Lincoln,S., Tyndall,G., Dickson,D. and Farrer,M.
  TITLE     Anatomical localization of leucine-rich repeat kinase 2 in mouse
            brain
  JOURNAL   Neuroscience 139 (3), 791-794 (2006)
   PUBMED   16504409
REFERENCE   9  (bases 1 to 8231)
  AUTHORS   Zimprich,A., Biskup,S., Leitner,P., Lichtner,P., Farrer,M.,
            Lincoln,S., Kachergus,J., Hulihan,M., Uitti,R.J., Calne,D.B.,
            Stoessl,A.J., Pfeiffer,R.F., Patenge,N., Carbajal,I.C.,
            Vieregge,P., Asmus,F., Muller-Myhsok,B., Dickson,D.W.,
            Meitinger,T., Strom,T.M., Wszolek,Z.K. and Gasser,T.
  TITLE     Mutations in LRRK2 cause autosomal-dominant parkinsonism with
            pleomorphic pathology
  JOURNAL   Neuron 44 (4), 601-607 (2004)
   PUBMED   15541309
REFERENCE   10 (bases 1 to 8231)
  AUTHORS   Takahashi,Y., Miyata,M., Zheng,P., Imazato,T., Horwitz,A. and
            Smith,J.D.
  TITLE     Identification of cAMP analogue inducible genes in RAW264
            macrophages
  JOURNAL   Biochim Biophys Acta 1492 (2-3), 385-394 (2000)
   PUBMED   11004510
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK052591.1, AC099704.7, AC158752.5, AK034413.1, AK014886.1,
            AK014938.1, AK050702.1 and BF660966.1.
            
            On May 4, 2007 this sequence version replaced NM_025730.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY792512.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1083              AK052591.1         2-1084
            1084-1084           AC099704.7         186229-186229
            1085-2897           AK052591.1         1086-2898
            2898-2935           AC158752.5         74559-74596
            2936-3153           AC158752.5         75043-75260
            3154-3376           AC158752.5         76665-76887
            3377-5169           AK034413.1         1-1793
            5170-5170           AC158752.5         103714-103714
            5171-6166           AK034413.1         1794-2789
            6167-6963           AK014886.1         226-1022
            6964-7786           AK014938.1         1036-1858
            7787-8225           AK050702.1         374-812
            8226-8231           BF660966.1         1-6                 c
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 46.07 cM"
     gene            1..8231
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /note="leucine-rich repeat kinase 2"
                     /db_xref="GeneID:66725"
                     /db_xref="MGI:MGI:1913975"
     exon            1..214
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            215..300
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            301..410
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            411..499
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            500..634
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            635..769
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            770..901
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1021
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1164
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1244
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1351
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1481
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1606
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1713
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1858
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1998
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2127
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2298
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2557
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            2558..2746
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..2865
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            2866..2935
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3153
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            3154..3404
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            3405..3553
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            3554..3647
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            3648..3834
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            3835..4016
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            4017..4246
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            4247..4374
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            4375..4593
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            4594..4795
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            4796..4884
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            4885..5072
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            5073..5227
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            5228..5374
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            5375..5566
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            5567..5713
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            5714..5814
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            5815..6005
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            6006..6166
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            6167..6337
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            6338..6438
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            6439..6633
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            6634..6827
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            6828..6900
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            6901..7085
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            7086..7238
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            7239..7447
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            7448..7519
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     exon            7520..8230
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8110..8115
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /note="hexamer: AATAAA"
     polyA_site      8134
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /note="major polyA site"
     regulatory      8205..8210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
                     /note="hexamer: AATAAA"
     polyA_site      8230
                     /gene="Lrrk2"
                     /gene_synonym="4921513O20Rik; 9330188B09Rik; cI-46;
                     D630001M17Rik; Gm927"
ORIGIN      
        1 gagcagctct gagagcagga gccgtcccag ctcgccgcag tccccgccgg ctgcaccatg
       61 gccagtggcg cctgtcaggg ctgcgaagag gaagaggagg aggaggctct gaagaagttg
      121 atagtcaggc tgaataatgt ccaggaaggc aagcagatcg agacgttgct tcagctcctg
      181 gaggacatgc tggtgttcac ctactcggac cgcgcctcca agttatttga agataaaaat
      241 ttccacgtgc ctctgttgat tgtcctggac tcctacatga gagttgccag tgtacagcag
      301 gcggggtggt cacttctgtg caaattaata gaagtctgtc cagggacatt gcaaagctta
      361 ataggacccc aggatattgg aaatgattgg gaagtccttg gtattcaccg gctgattctt
      421 aaaatgttaa ctgttcatca cgccaatgta aacctgtcaa tagttggact aaaagccttg
      481 gatctcctcc tagattcagg taaactcacc ttgctgatac tggatgaaga atgtgatatt
      541 ttcttgttaa tttttgatgc catgcacaga tattcagcca atgatgaagt ccaaaaactg
      601 ggatgcaaag ctttacacgt gctttttgag agagtttccg aggaacagct gactgagttt
      661 gtggagaaca aagattacac gatactgctg agtacgttcg gcagcttcag aagggacaag
      721 gagattgtgt accacgtact ttgctgcttg cattccctgg cggttacatg cagcaatgta
      781 gaggtcctca tgagtgggaa tgtccggtgc tacaatcttg tggtggaggc catgaaagcc
      841 ttccccacca atgaaaacat ccaagaggtg agctgctcct tgttccagaa gcttacatta
      901 ggtaactttt tcaacatcct ggtgttgaac gaagtgcatg tctttgtggt gaaagcggtc
      961 cgacagtatc ctgagaacgc agccttacag atctctgcac tcagctgttt agcactcctc
     1021 actgagacta ttttcttaaa ccaagacttg gaggaaagaa gtgagactca agagcagagc
     1081 gaagaggaag acagtgagaa gcttttctgg ctggaaccct gctataaagc cctggtgcgc
     1141 catcgaaagg acaaacacgt gcaggaggct gcctgctggg cactaaataa cctccttatg
     1201 taccagaaca gtttgcatga gaagatcgga gatgaagatg gccagttccc tgcgcacagg
     1261 gaagtgatgc tgtctatgct gatgcactct tcttccaaag atgtcttcca agcagctgca
     1321 catgctctgt ccactctctt ggaacaaaat gttaatttca ggaaaatcct gctggcaaaa
     1381 ggagtatacc tgaatgtctt ggaattgatg cagaagcatg cccatgcgcc tgaggtggca
     1441 gagagtggct gcaagatgct gagtcacctg tttgaaggaa gtaacccttc tctggataca
     1501 atggcagcag tggtccctaa aatactaaca gtgatgaaag cccacggaac gtctctgtca
     1561 gtccagctgg aggcgctgcg agctatcttg catttcgttg tgccaggact attggaagaa
     1621 tccagggagg actctcaatg cagaccaaat gtgctcagaa aacagtgttt caggactgac
     1681 atccacaagc tggttctagt cgctctgaac aggttcattg ggaatcctgg gattcagaaa
     1741 tgtggattga aagtaatctc ttctctcgcg caccttcctg atgccacaga gacattgtcc
     1801 ctgcaaggag cagttgactc agtcctccac accttacaga tgtatccaga tgaccaagaa
     1861 attcagtgtc tgggcttaca ccttatggga tgcttgatga caaagaagaa tttctgcata
     1921 gggacagggc acctcctggc aaaaattctg gcttccactt tgcagcgctt taaagatgtt
     1981 gctgaggtgc agactacagg attacagaca accctgtcaa tacttgagct gtcagtatct
     2041 ttctccaagc tgctagtgca ctattccttt gatgtggtga tatttcatca gatgtcttcc
     2101 agtgttgtag aacaaaagga tgagcagttc ctcaatctat gttgcaaatg ctttgcaaaa
     2161 gtggccgtgg atgatgagct gaaaaacacc atgctagaga gagcctgcga tcagaataac
     2221 agcatcatgg ttgaatgttt gctcctcttg ggagctgatg ccaaccaagt gaagggggca
     2281 acttctttaa tctatcaggt atgtgagaaa gagagcagtc ctaaattggt ggaactgttg
     2341 cttaatggtg gttgtcgtga acaagatgta cggaaggccc tgaccgtaag catccaaaag
     2401 ggcgacagcc aggtcatcag cttgctcctc aggaaacttg ccctggacct ggccaacaac
     2461 agcatttgcc ttggaggatt tggcatagga aaaattgatc cttcttggct tggtccttta
     2521 tttccagata agtcatccaa tttaaggaag caaacaaaca caggatctgt cctagcgagg
     2581 aaagtgctcc ggtatcagat gagaaacacc cttcaagaag gcgtggcctc aggcagtgac
     2641 ggcaagtttt ctgaagacgc gctggcgaaa tttggagaat ggacctttat tcccgactct
     2701 tctatggaca gtgtgtttgg ccagagcgat gatctggata gcgaaggcag cgagagctca
     2761 tttctcgtga agaggaagtc caactcaatt agtgtagggg aagtttacag agatctagct
     2821 ctgcagcgct gctcaccaaa tgctcagagg cattccaatt cgctgggtcc tgtttttgac
     2881 catgaagact tactgagacg aaaaagaaaa atactgtctt cagatgagtc tctcaggtcc
     2941 tcaaggctgc cgtcccatat gaggcaatca gatagctctt cttccctggc ttctgagaga
     3001 gaacacatca cgtcgttaga cctatctgcc aacgaactca aagatattga tgctctgagc
     3061 cagaagtgtt gcctcagtag ccacctggaa catctcacca aactggaact tcaccagaat
     3121 tcactcacga gcttcccaca gcagctgtgt gagactctga agtgtttgat acacttggat
     3181 ttgcacagta acaaattcac ctcatttccc tctttcgtgt tgaaaatgcc acgtatcacc
     3241 aacctagatg cctctcgaaa tgacatcggg ccaacagtag ttttagaccc tgcgatgaag
     3301 tgtccaagcc tcaaacagtt gaatctgtcc tataaccagc tctcttcaat cccagagaat
     3361 cttgcccaag tggtggagaa acttgagcag ctcctactgg aaggaaataa aatatccggg
     3421 atttgctctc ccctgagcct gaaggaactg aagattttaa atcttagtaa aaatcacatt
     3481 ccatccctac ctggagattt tcttgaggct tgttcaaaag tcgagagttt cagtgctcgc
     3541 atgaattttc ttgctgcaat gcctgcctta ccttcttcca taacgagctt aaaattgtct
     3601 cagaactctt tcacgtgcat tccagaagcg attttcagtc ttccgcactt gcggtccttg
     3661 gatatgagcc acaacaacat tgaatgtctg ccgggacctg cacattggaa gtctctgaac
     3721 ttaagggaac tcatttttag caagaatcag atcagcacct tagactttag tgagaaccca
     3781 cacgtgtggt caagagtaga gaaactgcat ctctctcata ataaactgaa agagattcct
     3841 ccagaaattg gctgccttga aaatctgacg tctctcgacg tcagttacaa cttggaactg
     3901 aggtccttcc caaatgaaat ggggaagttg agcaagatat gggatcttcc cttggacgga
     3961 ctgcatctga attttgactt taagcacgta ggatgcaagg ccaaagacat cataaggttt
     4021 ctacaacaac gtctgaaaaa ggctgtaccc tacaaccgaa tgaagctcat gattgtggga
     4081 aatacgggga gcggtaagac cactttactg caacaactca tgaaaatgaa gaaaccagaa
     4141 cttggcatgc agggtgccac agtcggcata gacgtgcgag actggtccat ccaaatacgg
     4201 ggcaaaagga gaaaggacct ggttctaaac gtgtgggatt ttgcaggtcg tgaggaattc
     4261 tacagcactc acccccactt catgacccag agagccctct acctggctgt ctatgatctc
     4321 agcaaggggc aggcagaggt ggacgccatg aagccctggc tcttcaatat caaggctcgt
     4381 gcctcttctt ccccggtgat tctggtgggc acacatttgg atgtttctga tgagaagcag
     4441 cggaaagcgt gcataagcaa aatcacgaag gaactcctaa ataagcgagg attccccacc
     4501 atccgggact accactttgt gaatgccacc gaggagtcag atgcgctggc aaagcttcgg
     4561 aaaaccatca taaatgagag ccttaatttc aagatccgag atcagcctgt ggttgggcag
     4621 ctaattccag attgctacgt agaactggag aaaatcattt tatcagagcg gaaagctgtg
     4681 ccgactgagt ttcctgtgat taaccggaaa cacctgttac agctcgtgaa cgaacatcag
     4741 ctgcagctgg atgagaacga gctcccacac gccgttcact tcctaaatga gtcgggagtt
     4801 cttctgcatt ttcaagaccc tgccctgcag ctaagtgacc tgtactttgt ggaacccaag
     4861 tggctttgta aagtcatggc acagatcttg acagtgaagg tagacggctg tctgaaacat
     4921 cctaagggca tcatttcccg gagagatgtg gaaaaattcc tttcaaagaa gaagcgattc
     4981 ccgaagaact atatgatgca atactttaaa ctattagaaa aatttcagat cgcattgcca
     5041 ataggggaag aatatcttct ggttccaagc agcttgtctg accacaggcc agtgatagag
     5101 ctcccccact gtgagaactc tgagatcatc atccggctgt acgaaatgcc gtactttccc
     5161 atgggatttt ggtcaagatt gattaaccga ttacttgaaa tctcaccctt catgctttct
     5221 ggcagagaga gagcactacg ccctaacagg atgtattggc ggcaaggcat ctacttgaat
     5281 tggtctccag aagcatactg tctggtaggc tctgaagtct tagacaatcg acctgagagt
     5341 ttcttgaaaa tcacagttcc gtcttgtaga aaaggttgta ttcttctggg ccgagttgtg
     5401 gatcatattg actcactcat ggaagaatgg tttcccgggt tactggagat tgacatttgt
     5461 ggggaaggag aaactctgtt gaagaaatgg gcattgtaca gttttaatga tggtgaagaa
     5521 catcagaaga tcttgcttga tgagttgatg aagaaggctg aagaaggaga cctgttaata
     5581 aatccagacc aaccaaggct cactattcca atatcccaga ttgctccgga cttgatcttg
     5641 gctgacctgc ctagaaatat catgttgaac aatgatgagt tggaatttga ggaagcacca
     5701 gagtttctct taggcgatgg aagttttgga tccgtttatc gagctgccta cgaaggagag
     5761 gaagtggctg tgaagatttt taataagcac acatctctta ggctgttaag acaagagttg
     5821 gtggtccttt gtcaccttca ccaccccagc ctgatatcct tgctggcggc tggtattcgt
     5881 cctcggatgt tggtaatgga gttggcctcc aaaggttcct tggatcgcct gctgcagcag
     5941 gacaaagcca gcctcaccag aaccctccag cacaggatcg cgttgcatgt ggccgacggc
     6001 ctgaggtatc tccactcagc catgattatt taccgtgacc tgaagcccca caatgtgctg
     6061 ctttttaccc tgtatcccaa tgctgccatc attgcgaaga ttgcggacta cgggatcgca
     6121 cagtactgct gcaggatggg aataaagaca tcagagggca ccccagggtt ccgggcacct
     6181 gaagttgcca gggggaatgt catttataac caacaggccg atgtttattc ttttggctta
     6241 ctacttcacg atatttggac aactgggagt aggattatgg agggtttgag gttcccaaat
     6301 gagtttgatg agttagccat acaagggaag ttgccagatc cagttaaaga atatggctgt
     6361 gccccatggc ctatggttga gaagttaatt acaaagtgtt tgaaagaaaa tcctcaagaa
     6421 agacccactt ctgcccaggt ctttgacatt ttgaattcgg ctgaattaat ttgcctcatg
     6481 cgacacattt taatacctaa gaacatcatt gttgaatgca tggttgccac gaatctcaat
     6541 agcaagagtg cgactctctg gttgggatgt gggaacacag aaaaaggaca gctttcctta
     6601 tttgacttaa acacggaaag atacagctat gaggaagttg ctgatagtag aatactgtgc
     6661 ttggctttgg tgcatctcgc tgctgagaaa gagagctggg ttgtgtgcgg gacacagtct
     6721 ggggctctcc tggtcatcaa tgttgaagag gagacaaaga gacacaccct ggaaaagatg
     6781 actgattctg tcacttgttt gcattgcaat tcccttgcca agcagagcaa gcaaagtaac
     6841 tttcttttgg tgggaactgc tgatggtaac ttaatgatat ttgaagataa agccgttaag
     6901 tgtaaaggag ctgccccctt gaagacacta cacataggcg atgtcagtac gcccctgatg
     6961 tgcctgagcg agtccctgaa ttcatctgaa agacacatca catggggagg gtgtggcaca
     7021 aaggtcttct ccttttccaa tgatttcacc attcagaaac tcatcgagac aaaaaccaac
     7081 cagctgtttt cttacgcagc tttcagcgat tctaacatca tagcgctggc agtagacaca
     7141 gccctgtata ttgccaagaa aaacagccct gtcgtagagg tgtgggacaa gaaaacagaa
     7201 aagctctgtg aattaataga ctgtgtgcac ttcttaaagg aggtgatggt aaaactaaac
     7261 aaggaatcga aacatcagct gtcctactct gggagggtga aggccctctg cctgcagaag
     7321 aacacggctc tctggatcgg aactggagga ggccacatct tactcctgga tctttctact
     7381 cggcgagtta tccgcaccat tcacaatttc tgtgattctg tgagagccat ggccacagca
     7441 caattaggaa gccttaagaa tgtcatgctg gttttggggt acaagcggaa gagtacagag
     7501 ggtatccaag aacaaaaaga gatacaatct tgtttgtcta tttgggacct caatcttcca
     7561 cacgaggtgc aaaatttaga aaaacacatt gaagtaagaa cagaattagc tgataaaatg
     7621 aggaaaacat ctgttgaata gaaagacatc aggcagtctc gatgttatat tgaataagac
     7681 atcagacatc ctcgtcacta tattgaaaag gacatcagac atcctcgcca atatgttaga
     7741 aaatgtactc ttctttttaa aatatatttt taaaatgttt acattgaaaa gagtatgcct
     7801 attctttaca aagttcatat gtatatgaag gaatgtgtat gtcttatgtt taatttaata
     7861 tatgtaaaaa tatttatcag taaatatgtt ttaaaaaact atttaattta gcattatatt
     7921 ttctatactc cttaactaat ttgaagggat aaacaaaaga aatctacaaa gcatttaatt
     7981 tcagtattta tactaaaatt aataaaaata tcatgtttgt tttgctatgt attgtgatga
     8041 taaagcctat tttaaattgt tgattaagac acagatgttg cttgattatc tatggactca
     8101 gcggagtaga ataaaatatc tggtcaattt ccaagtaaga gactctttca tatcttgttt
     8161 tcaagtgaat tatcatcatt aatgtaaact gtcatatttt cactaataaa gatttttgtt
     8221 agctcaggaa a
//
