LOCUS       NM_146191               7467 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus leucine-rich repeat kinase 1 (Lrrk1), mRNA.
ACCESSION   NM_146191 XM_485946
VERSION     NM_146191.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Huang,G., Bloodgood,D.W., Kang,J., Shahapal,A., Chen,P.,
            Kaganovsky,K., Kim,J.I., Ding,J.B. and Shen,J.
  TITLE     Motor Impairments and Dopaminergic Defects Caused by Loss of
            Leucine-Rich Repeat Kinase Function in Mice
  JOURNAL   J Neurosci 42 (23), 4755-4765 (2022)
   PUBMED   35534227
REFERENCE   3  (bases 1 to 7467)
  AUTHORS   Rios,J.J., Denton,K., Russell,J., Kozlitina,J., Ferreira,C.R.,
            Lewanda,A.F., Mayfield,J.E., Moresco,E., Ludwig,S., Tang,M., Li,X.,
            Lyon,S., Khanshour,A., Paria,N., Khalid,A., Li,Y., Xie,X.,
            Feng,J.Q., Xu,Q., Lu,Y., Hammer,R.E., Wise,C.A. and Beutler,B.
  TITLE     Germline Saturation Mutagenesis Induces Skeletal Phenotypes in Mice
  JOURNAL   J Bone Miner Res 36 (8), 1548-1565 (2021)
   PUBMED   33905568
REFERENCE   4  (bases 1 to 7467)
  AUTHORS   Malik,A.U., Karapetsas,A., Nirujogi,R.S., Mathea,S., Chatterjee,D.,
            Pal,P., Lis,P., Taylor,M., Purlyte,E., Gourlay,R., Dorward,M.,
            Weidlich,S., Toth,R., Polinski,N.K., Knapp,S., Tonelli,F. and
            Alessi,D.R.
  TITLE     Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct
            Rab proteins and are regulated by diverse mechanisms
  JOURNAL   Biochem J 478 (3), 553-578 (2021)
   PUBMED   33459343
  REMARK    GeneRIF: Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate
            distinct Rab proteins and are regulated by diverse mechanisms.
REFERENCE   5  (bases 1 to 7467)
  AUTHORS   Si,M., Goodluck,H., Zeng,C., Pan,S., Todd,E.M., Morley,S.C.,
            Qin,X., Mohan,S. and Xing,W.
  TITLE     LRRK1 regulation of actin assembly in osteoclasts involves serine 5
            phosphorylation of L-plastin
  JOURNAL   J Cell Biochem 119 (12), 10351-10357 (2018)
   PUBMED   30136304
  REMARK    GeneRIF: Our data suggest that targeted disruption of L-plastin
            increases trabecular bone volume, and phosphorylation of Ser5 in
            L-plastin in the Lrrk1 signaling pathway may in part contribute to
            actin assembly in mature osteoclasts.
REFERENCE   6  (bases 1 to 7467)
  AUTHORS   Xing,W., Liu,J., Cheng,S., Vogel,P., Mohan,S. and Brommage,R.
  TITLE     Targeted disruption of leucine-rich repeat kinase 1 but not
            leucine-rich repeat kinase 2 in mice causes severe osteopetrosis
  JOURNAL   J Bone Miner Res 28 (9), 1962-1974 (2013)
   PUBMED   23526378
  REMARK    GeneRIF: Skeletal phenotypes in Lrrk1 and Lrrk2 knockout (KO) mice,
            were investigated.
REFERENCE   7  (bases 1 to 7467)
  AUTHORS   Giesert,F., Hofmann,A., Burger,A., Zerle,J., Kloos,K., Hafen,U.,
            Ernst,L., Zhang,J., Vogt-Weisenhorn,D.M. and Wurst,W.
  TITLE     Expression analysis of Lrrk1, Lrrk2 and Lrrk2 splice variants in
            mice
  JOURNAL   PLoS One 8 (5), e63778 (2013)
   PUBMED   23675505
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 7467)
  AUTHORS   Zechel,S., Meinhardt,A., Unsicker,K. and von Bohlen Und Halbach,O.
  TITLE     Expression of leucine-rich-repeat-kinase 2 (LRRK2) during embryonic
            development
  JOURNAL   Int J Dev Neurosci 28 (5), 391-399 (2010)
   PUBMED   20403420
REFERENCE   9  (bases 1 to 7467)
  AUTHORS   Biskup,S., Moore,D.J., Rea,A., Lorenz-Deperieux,B., Coombes,C.E.,
            Dawson,V.L., Dawson,T.M. and West,A.B.
  TITLE     Dynamic and redundant regulation of LRRK2 and LRRK1 expression
  JOURNAL   BMC Neurosci 8, 102 (2007)
   PUBMED   18045479
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 7467)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY024941.1, BC072664.1, AK040153.1, AK076432.1 and BC066159.1.
            
            On or before Dec 20, 2004 this sequence version replaced
            XM_485946.1, NM_146191.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147469.1, SRR10223970.9522.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                BY024941.1         12-62
            52-3679             BC072664.1         1-3628
            3680-4761           AK040153.1         185-1266
            4762-7449           AK076432.1         89-2776
            7450-7467           BC066159.1         1972-1989
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 35.57 cM"
     gene            1..7467
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /note="leucine-rich repeat kinase 1"
                     /db_xref="GeneID:233328"
                     /db_xref="MGI:MGI:2142227"
     exon            1..122
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            123..337
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..6285
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="leucine-rich repeat serine/threonine-protein
                     kinase 1"
                     /protein_id="NP_666303.3"
                     /db_xref="CCDS:CCDS21344.1"
                     /db_xref="GeneID:233328"
                     /db_xref="MGI:MGI:2142227"
                     /translation="MAGTSQRPPSMYWCVGTEGLAVCPGPAMETHNGAEDMGSKLSLP
                     GGSSTVQCPSMEEIHTAYKQRNLSRARDLLRGVCEESESSQEKGQLLSIAAAHGDLET
                     VQFLLTEKRVELPTEPTDDNPAVVAAHFGHAEVVRELLESLPGPCTPQRLLNWMLALA
                     CQRGHLEVVKLLVLTHGADPENYAVRKNEFPVIVRLPLYAAIKAGNEDIAIFLLRHGA
                     YFCSYILLDSPEPSKHLLRKYFIEASALPSSYPGKIALRVKWSHLKLPWVDLDWLLDI
                     SCQITELDLSANCLPSLPSIIPWGLINLKKLNLSNNQLGELPCVQSSDEIICSRLLEI
                     DISSNKLSHLPPGFLHLSKLQKLTASKNYLERLFEEENATNWIGLRKLQELDLADNRL
                     TELPVQFMHSFKSLTSLNVSRNNLKSFPDPWSCPLKCCKASKNALESLPDKMAVFWKS
                     HLRDADFSENSLKEVPLGLFQLDALMFLRLQGNQLLSLPPQEKWTCTQLKTLDLSRNQ
                     LGKNEDGLKTKRISLFTTRGRQRSGTETASMLEFPAFLSESLEVLCLNDNHLDAVPPS
                     VCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNISNVPAEVRKEGPKATLS
                     FLRAQLRKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQLAHSEATIRTTKWELQR
                     PAGSKAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWL
                     LNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITF
                     KHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLA
                     EQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSH
                     GLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYF
                     QFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQR
                     FIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQE
                     GLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFP
                     ALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDMEDCVLTAIERDFIS
                     CPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQARY
                     QGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIV
                     SLIGISIHPLCFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLH
                     KKNIIFCDLKSDNILVWSLSAKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIR
                     PRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIVKKLSKGIRPVLGQPEEVQFHR
                     LQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAFFSSQSQEYTVVF
                     WDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKG
                     MCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSY
                     LCSHTANRSKFCIPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRR
                     LEPYAAPSMVTSLVCSSDCRGEEMVWCLDDKANCLVMYHSATYQLCARYFCGDPNPLR
                     DTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYSEELGTQILTHQDSLTDYCSMSS
                     YSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRTEHDLSPMDGE
                     TFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVL
                     VDAAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR"
     exon            338..501
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            502..673
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            674..853
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            854..1002
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1229
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1357
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1521
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1659
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..1772
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1849
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            1850..1979
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2092
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2307
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2472
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..2645
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            2646..2766
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            2767..2926
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3203
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            3204..3375
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            3376..3539
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            3540..3679
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            3680..3929
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            3930..4096
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            4097..4294
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            4295..4572
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            4573..4767
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            4768..4919
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            4920..5037
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            5038..5154
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            5155..6005
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            6006..6107
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     exon            6108..7456
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7435..7440
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /note="hexamer: AATAAA"
     polyA_site      7456
                     /gene="Lrrk1"
                     /gene_synonym="C230002E15Rik; D130026O16Rik; mKIAA1790"
                     /note="major polyA site"
ORIGIN      
        1 ggattgagga agtgcgtctg gcccccggcg gccagagcat tgcagggcag agtacggtag
       61 gaccggcagg ccccggccct ctgatgtcgc cgatccgcgg cttccagacc ctgccgccgc
      121 aggatgagcg tgtgtctgca gcgacatgac taggtgtccg tgcttcggcc catctgcaaa
      181 gtggtggttg cggtgacaac agcgagactt tcctttggag atctgctgcc tggggagttg
      241 atggctggca cgtcacagcg gccacccagt atgtactggt gtgtggggac agaaggatta
      301 gctgtgtgtc caggacctgc catggagacc cataatggtg ccgaagacat gggcagcaaa
      361 ctgtctttac caggtggcag ctccacggtg cagtgcccca gcatggaaga aatccacact
      421 gcgtacaagc agaggaacct ctcccgagcc cgagacctac tcagaggggt ttgtgaagag
      481 agcgaatcct cacaggagaa gggccagctg ctgagcatcg cggcagccca cggggatctg
      541 gagactgttc agttcctgct cactgagaag cgcgtggagc tgccgacgga gcccaccgat
      601 gacaacccag ctgtggtggc agcgcatttt ggacacgccg aagtagtacg tgaactacta
      661 gagtccttgc caggtccctg taccccccag cggctactga actggatgct ggccctggct
      721 tgccagcgag ggcacctgga ggttgtgaag ctgctggtcc tgacacatgg ggctgacccg
      781 gagaactatg ccgtcaggaa gaatgagttc cccgtcattg tgcggttgcc cctctatgcg
      841 gccatcaagg cagggaatga agacattgcc atattcctgc ttcggcacgg agcctatttc
      901 tgctcctaca tcctgctgga tagtcctgag ccaagcaaac atctgctcag gaagtatttc
      961 atcgaagcca gcgccctgcc cagcagctat cccgggaaaa tcgcacttcg tgtgaaatgg
     1021 tcccatctga agttgccctg ggtggacctg gactggctcc tagacatctc ctgccagatc
     1081 acagagcttg acctgtctgc caactgcctg ccttcgctcc cttccatcat cccttgggga
     1141 ctcatcaatc taaagaagct gaatctctca aacaaccagc tgggggagct gccctgcgtg
     1201 cagtcctccg acgaaatcat ctgctccagg ttacttgaaa ttgacatctc cagcaacaaa
     1261 ctgtctcacc tccctcctgg attcctgcac ctctcaaaac ttcaaaagct gactgcttcc
     1321 aaaaactacc tggagcggct gtttgaagaa gaaaacgcca ctaactggat cggcctgcgg
     1381 aagctgcaag aactcgatct agctgacaac aggctgacag agctccctgt ccaatttatg
     1441 cactccttca agtctctcac ctctctgaat gtctccagga acaacctgaa gagcttcccg
     1501 gatccctggt cctgcccttt gaaatgttgt aaagcctcca aaaatgccct ggaatcgctg
     1561 ccagacaaaa tggctgtctt ttggaaaagc caccttcggg atgcagattt ctctgagaac
     1621 tctctgaaag aggtgcccct gggattgttc cagcttgacg ccctcatgtt cttgaggtta
     1681 cagggaaacc agctgttgtc gctgccacct caagagaagt ggacctgcac ccaactcaag
     1741 accctggacc tctccagaaa ccagctcggc aaaaatgaag atgggcttaa aacaaagagg
     1801 atctccctgt tcaccaccag agggcgccag cgttctggaa ctgagacagc ctccatgttg
     1861 gaattcccag ctttcctaag tgagtctttg gaggtccttt gtctgaatga caaccatctt
     1921 gatgctgttc ccccatcggt ctgcctgctg aagaacctct cagagctcta cttgggcaat
     1981 aaccctggcc tccgagagct ccctccggag ctaggccagc tggggaacct ctggcaactg
     2041 gacattgaag atctgaacat tagcaacgtg cctgcggagg tcaggaaaga aggcccgaaa
     2101 gcaacactgt ctttcctgcg tgctcagctg cgcaaagcag agaagtgtaa gctgatgaag
     2161 atgatcctcg tgggtccccc gcgccagggc aagtccacac tcctggagat cttacagacc
     2221 gggaaggccc cgcagctagc acacagtgaa gccaccatca ggaccaccaa gtgggagctc
     2281 cagagaccag caggctccaa agccaaggtt gagtccgtag agttcaacgt ctgggacatc
     2341 gggggtccgg ccagcatggc cacggtcaat cagtgcttct tcacagacaa ggcgctgtat
     2401 gtggtggtct ggaacctggc actgggagag gaagccgtgg ccaacctcca gttctggctg
     2461 ctcaacattg aggccaaggc cccaaatgcc gttgtgctgg tggtcgggac acacctggac
     2521 ctaatcgaag ctaagttccg agtggagagg atagcaaccc tgcgtgccta cgtgctggcc
     2581 ctctgccgct cgccatcggg ctccagggct acgggcttcc cggacatcac tttcaaacac
     2641 ttgcatgaga tctcctgcaa gaatctggaa gggcaggagg ggctgcggca actgatcttc
     2701 cacgtcacgt gcaacatgaa ggatgtgggt agcactatcg gctgtcaaaa actcgccggc
     2761 aggctgatcc ccaggagcta cataagccta caggaggccg tgctggctga gcaacagcgc
     2821 cgcagtctgg gtgaccaagt acagtatctg acggacaggc agctggacca gctggtagag
     2881 cagacgcctg gcaatgatat caaagactat gaagacctgc agtctgccat cagcttcctc
     2941 atagaaactg ggaccctgct gcactttcca gacacgagcc atggcctaag gaacctctac
     3001 ttcctggatc ccatctggct ctctgaatgc ctgcagagga tctttaacat caagggctcg
     3061 cggtcagtgg caaagaatgg ggtgatccaa gcggaagacc tcaggatgct gctggtgggg
     3121 acaggcttca cacagcagac tgaggagcaa tacttccagt tccttgccaa gttcgagatt
     3181 gccctccctg ttgccaatga cagctacctc ctaccacacc tccttccatc caagcctggg
     3241 ctggacaccc acagcatgcg gcacccaatg gctaacacca tccagcgggt gtttaagatg
     3301 agcttcgtgc ctgttggctt ctggcaacgg ttcatagcac ggatgctaat cagcttggct
     3361 gagatggacc tgcagctttt tgaaaacaag aagaatacga aaagcaggaa ccggaaagtc
     3421 actatttaca gttttacagg gagccagaga aaccgctgca gtacattccg agtccgaaga
     3481 aatcagacca tctactggca ggaagggctg ctggtcactt tcgatggggg ctacctcagt
     3541 gtggaatcct cagatgtgaa ctggaaaaag aaaaaaagcg gaggaattaa aatcatctgc
     3601 cagtcagaga tgagggactt ctcggcaatg gcttttatca cagaccatgt caactccctg
     3661 attgaccagt ggttccctgc cctgacggcc acagaaagtg acgggacccc actgatggag
     3721 cagtacgtac cctgccctgt ctgtgaagca tcctgggccc agcatgcaga tccaaacgag
     3781 aggtcagaga gcgtgcagta ctttgatatg gaagactgtg tgctcacagc catcgaacgg
     3841 gacttcatct cctgccccag acacccagac ctcccagtac cacttcagga gcttgtacca
     3901 gagctgttca tgactgactt cccagccagg ctcttcctgg agaacagcaa gctggagcac
     3961 acggagggtg agaacagcat tctgggccaa ggtggcagtg gcacagtcat ctaccaggcc
     4021 cggtaccagg gccagcctgt ggctgtgaag aggttccata tcaagaagtt caagaattct
     4081 gctaatgccc cagcagacac catgctgcgg cacctgagag ccatggatgc catgaagaac
     4141 ttctcagatt tccgtcagga ggccagcatg ctgcatgcct tgcagcatcc ttgcatcgtg
     4201 tcactcatcg gcatcagcat ccacccgctc tgcttcgcct tggagctggc cccgctgggc
     4261 agtctcaaca ccgtgctatc tgagaatgcc aaagattctt cctttatgcc cctgggacac
     4321 atgctcaccc aaaagatagc ctaccagatt gcctcgggcc tggcctacct gcacaagaaa
     4381 aacatcatct tctgtgacct caagtcggac aacatcctag tgtggtcgct gagcgccaag
     4441 gagcatatca acatcaaact gtcagactac ggcatctcca ggcagtcctt tcacgagggg
     4501 gccctgggtg tggagggcac cccaggttac caggccccag agatcaggcc tcgcatcgtg
     4561 tatgacgaga aggtagatat gttctcctac ggcatggtgc tatacgagct gctgtcagga
     4621 cagcgtcctg cactgggcca ccaccaactt cagattgtca agaagttgtc caagggcatc
     4681 cgcccagttc tggggcagcc ggaggaagtt cagttccatc ggctgcaggc gctcatgatg
     4741 gagtgctggg acacaaagcc ggagaagcgc cccctggcct tgtccgtggt gagccagatg
     4801 aaagacccaa cctttgccac cttcatgtac atgctgccct gtgggaagca gtcggccttc
     4861 ttctcatccc agagccagga gtacactgtg gtgttctggg atgggaagga ggagtccagg
     4921 aattacacgg tggtcaacac agagaagggc cttctggaag tacagaggat gacctgcccc
     4981 gggatgaagt tgagctgtca gctcaaggtc cagagctcag tgtggatagc cacggaggac
     5041 cagaaaatct atatctacag ccttaagggt atgtgcccat taagcgtgcc ccagcaggcc
     5101 ctggacaccc cagctgtcgt cacctgtttc ttggcagtac ctgttatcaa aaagaactcc
     5161 ttcctggtgc tggcaggcct ggctgacgga ctcgtggctg tgtttcctgt ggcacggggc
     5221 accccaaaag aaagctgctc ctacctgtgc tcccacacag ccaacaggtc caagttctgc
     5281 atccccgacg aagatgcacg gcagaaccct tacccagtga aggcaatgga agtggtcaac
     5341 agtgggtctg aggtctggta cagcaacggg cctggcctcc tcgtcattga ctgcaccatc
     5401 ctggacatca gcaggcggct ggagccctac gcagctccgt ccatggtcac gtcacttgtg
     5461 tgcagctcag actgcagagg agaggagatg gtctggtgcc tcgacgacaa ggccaattgc
     5521 ttagtgatgt accactcagc cacctaccag ctctgtgccc ggtacttctg tggggacccc
     5581 aatcctctca gggacacgtt ttctgtgcag ccttcagtcc tagaaacccc aggcagtcac
     5641 aaaaccacct caaaggggcc tgtggaagag tgcatcgcgg atgtgagcat catgtacagc
     5701 gaggagctgg gcacgcagat cctcacccac caggactcgc tcaccgacta ctgctccatg
     5761 tcctcttatt cttcgtcccc accccaccaa gatcctaggt ccccttccag tctgcccagc
     5821 tccctcacca gctattccag tgtgcctttc tctgccaact atgaagactc agacaggctg
     5881 caggagccca gtgtcacctc tgacagaact gagcatgacc tgagccccat ggatggggag
     5941 acgttcagcc agcacctgca ggctgtgaag gtgcttgctg tcaaagacct catttgggtt
     6001 cctagacatg gaggagatat cattgtcatc ggcctggaga aggattcagg tgcccagcgg
     6061 ggcagagtca tcgctgtttt aaaagcccga gagctcaatc ggcacggggt tctggtggat
     6121 gccgcagtgg tggcaaagga caccgtagtg tgcagctttg caaatgaaaa cacagagtgg
     6181 tgcctggctg tctggagggg ctggggcgcc agggagtttg acatcttcta ccagtcctat
     6241 gaggagctgg gccggctgga ggcttgtact cgcaagagaa ggtaatgcct atggaagggc
     6301 ctccgagtgg agccagcagg tccgagccgc tgcctgaccc ctcaactcca gccctcctag
     6361 ggcacagaag acaagtgggt tctttgctaa accttctgct aagaagggct gagaagctag
     6421 gagctactgg tagtttggga gttctcaggt tctgtgggga aagttaccag cccatgggaa
     6481 gccacaggga ttggttgaga gaggcctcct cttgcctctt ccataggctt ccaggagccg
     6541 gggagagtga ccccactcca agccttcccg gcataatgaa tgggcctgtc cgttttttat
     6601 cgccaggcct cagatggaat tacactacag agtcttggtt gaggagcagt tcagggtaag
     6661 gtgggaggag ggctgggacc cctggccctc cttcccccaa agaccccagt ctttcagcct
     6721 gtttcacaag gctctgaatc cctggagtgt gaggcccagc ttggtggaac tgagatcgca
     6781 tctcctgctc ggcaaaggct gcacatggga aaccccagaa ggacctgggc aggcagcatt
     6841 gcccaatagc agagatgaat ggattccttc aagagtcatg gccggaaagc ccagtgcaag
     6901 ggtccctggt ctccctttac attcttctcc cccgacaaaa ggaaggagaa acccaatctc
     6961 tgggcccaga gaaagggcct gtgtttatga cttggtaaaa taaacttgga cacagctgga
     7021 gcacaagccc tgtttgtttg cacatcataa tcttgtttat cactttaaca aattaagtaa
     7081 tatctcagcg gttaggcctg gttgtgaaat cccatcgtat gggacttata ctgaattatg
     7141 tatttgccaa acatttactg cccacgtgga cagcagatga agaaaaggca agttgttaac
     7201 agggtgtatg ggcacaacgg ggaccatttt ataaatcctg tacacacgtg tgcacacaca
     7261 cccacatctt tgccgagcct cgcccttaga ggatgcctaa cctagaactc acgtttgaat
     7321 gtgagactgt atttggctcg ggttactgaa ccttttctag aattaaatta taaaaaagaa
     7381 aacgttaaga tgtgccgcgg ggtgcgcatg ctggtgagac tggtgtcatc tgagaataaa
     7441 cagacttaga cgaaaaaaaa aaaaaaa
//
