LOCUS       NM_028175               3878 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus leucine rich repeat containing 8 family, member E
            (Lrrc8e), mRNA.
ACCESSION   NM_028175 XM_146206
VERSION     NM_028175.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Lopez-Cayuqueo,K.I., Planells-Cases,R., Pietzke,M., Oliveras,A.,
            Kempa,S., Bachmann,S. and Jentsch,T.J.
  TITLE     Renal Deletion of LRRC8/VRAC Channels Induces Proximal Tubulopathy
  JOURNAL   J Am Soc Nephrol 33 (8), 1528-1545 (2022)
   PUBMED   35777784
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Zhou,C., Chen,X., Planells-Cases,R., Chu,J., Wang,L., Cao,L.,
            Li,Z., Lopez-Cayuqueo,K.I., Xie,Y., Ye,S., Wang,X., Ullrich,F.,
            Ma,S., Fang,Y., Zhang,X., Qian,Z., Liang,X., Cai,S.Q., Jiang,Z.,
            Zhou,D., Leng,Q., Xiao,T.S., Lan,K., Yang,J., Li,H., Peng,C.,
            Qiu,Z., Jentsch,T.J. and Xiao,H.
  TITLE     Transfer of cGAMP into Bystander Cells via LRRC8 Volume-Regulated
            Anion Channels Augments STING-Mediated Interferon Responses and
            Anti-viral Immunity
  JOURNAL   Immunity 52 (5), 767-781 (2020)
   PUBMED   32277911
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC080783.1 and AC123029.3.
            
            On Jul 8, 2009 this sequence version replaced NM_028175.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC080783.1, CF915317.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-280               BC080783.1         1-280
            281-281             AC123029.3         108666-108666
            282-2374            BC080783.1         282-2374
            2375-2511           AC123029.3         112870-113006
            2512-3306           BC080783.1         2512-3306
            3307-3878           AC123029.3         113802-114373
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 1.99 cM"
     gene            1..3878
                     /gene="Lrrc8e"
                     /gene_synonym="1810049O03Rik"
                     /note="leucine rich repeat containing 8 family, member E"
                     /db_xref="GeneID:72267"
                     /db_xref="MGI:MGI:1919517"
     exon            1..179
                     /gene="Lrrc8e"
                     /gene_synonym="1810049O03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            180..322
                     /gene="Lrrc8e"
                     /gene_synonym="1810049O03Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..2572
                     /gene="Lrrc8e"
                     /gene_synonym="1810049O03Rik"
                     /note="leucine-rich repeat-containing protein 8E"
                     /codon_start=1
                     /product="volume-regulated anion channel subunit LRRC8E"
                     /protein_id="NP_082451.2"
                     /db_xref="CCDS:CCDS22079.1"
                     /db_xref="GeneID:72267"
                     /db_xref="MGI:MGI:1919517"
                     /translation="MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCT
                     LQVTQDKIICLPSHESRENISGAPCQQLLPQGISEQMGGLRELSGLKNNLDLQQYSFI
                     NQLCYETALHWYAKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSP
                     WTTRALSEVSGENHKGPASGRAMVTTVTTTGAGSGKVGEGEKEKVLIEPEKVVSEPPV
                     VTLLDKKEGEQAKALFEKVKKFRVHVEEGDILYSMYIRQTVLKVCKFFAILVYNLIYV
                     EKISFLVACRVETSEITGYASFCCNHTKAHLFSKLAFCYISFVCVYGITCLYTLYWLF
                     HRPLKEYSFRSVREETGMNDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRL
                     KQLNLNHEWTPEKLRQKLQRNMRGRLELSLCMLPGLPDTVFELSEVEALRLEAICDIS
                     FPPGLSQLVNLQELSLLHSPARLPFSSQIFLRDRLKVICVKFEELREVPLWVFGLRGL
                     EELHLEGLFPPEMARGATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHN
                     DGARLLALNSLKKLAVLRELELVACGLERIPHAIFSLGALQELDLKDNHLRSIEEILS
                     FQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSHNKLETLPTQLGQCFGLRLLDL
                     SHNGLRSLPPELGLLQSLQHLALSYNALESLPDELFFCHKLRTLLLGYNHLTQLSPDV
                     AALQALSRLELKGNRLETLPEELGDCKGLKKSGLLVEDTLYEGLPAEVREKMEEE"
     exon            323..3878
                     /gene="Lrrc8e"
                     /gene_synonym="1810049O03Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3855..3860
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrc8e"
                     /gene_synonym="1810049O03Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3878
                     /gene="Lrrc8e"
                     /gene_synonym="1810049O03Rik"
                     /note="major polyA site"
ORIGIN      
        1 ctgatagcgt ctgtagtgag gcggggccga acaatcccgg gggcggggcg gggcggctcc
       61 tggcccagcc cgccctgact caccctctct ggcccgcccg ggaactctgc gggagtccgg
      121 gtcgatctac tcccaggcat ctagaagttg ggccccggag ttccatccct gagcagtgag
      181 caggatgatc ccagtggcgg agttcaagca gttcacagag cagcagcctg cattcaaggt
      241 gctcaaaccc tggtgggatg ttctggctga gtacctcacc gtggctatgc tcatgatcgg
      301 ggtattcggg tgcaccctcc aggtgacaca ggacaagatc atctgcttgc ctagccacga
      361 atctcgtgag aacatatcgg gggctccatg ccagcagctt ttgcctcaag gaatctctga
      421 gcagatgggg ggtctccgag agctcagtgg cctcaaaaac aacctagacc tccagcagta
      481 tagctttatc aaccagctct gctatgagac tgccctccac tggtatgcca aatacttccc
      541 ctacttggtg gttatccata ctctcatctt tatggtctgt accagctttt ggttcaaatt
      601 ccctggtacc agctccaaaa tagaacactt catctccatc ctgggcaagt gctttgattc
      661 tccatggacc actcgggcct tgtccgaggt gtctggagaa aatcacaagg gcccagcttc
      721 tggacgggcc atggtgacca cggtaaccac cacaggggca gggtcgggga aggtgggtga
      781 aggtgagaag gagaaagtgc tcattgagcc cgaaaaggtg gtgtctgagc ctccagttgt
      841 tactctgctg gataagaagg agggcgagca agcgaaggcc ctattcgaga aggtcaaaaa
      901 gttccgagtg cacgtggaag agggtgacat cctctattcc atgtacatcc ggcagacagt
      961 gctcaaagtg tgtaagtttt ttgccatcct ggtttacaac ctgatctatg tggagaagat
     1021 cagctttctg gtggcctgta gggtggagac atcagagatc acggggtatg ccagtttctg
     1081 ctgcaaccac accaaggccc acctcttttc taagctggcc ttctgctata tttcctttgt
     1141 gtgtgtctat ggcatcacct gcctgtatac actctactgg ctcttccata ggcccctaaa
     1201 agagtactcc ttccgttctg tgcgtgagga gacagggatg aacgatatcc cggatgttaa
     1261 gaatgacttt gccttcatgc tccacctcat tgaccagtac gactccctct attccaaacg
     1321 ctttgcagtc ttcctctctg aagtcagtga gagccgcctt aagcaactca acctcaacca
     1381 tgagtggaca cctgagaagc tgaggcagaa gctgcagcgt aacatgcgtg gccggctgga
     1441 gctgagcctc tgcatgctcc ctggactacc tgacaccgtc tttgagctca gcgaggtgga
     1501 ggcactcagg ctggaggcca tctgtgacat ctcctttccc cctgggctct cccagcttgt
     1561 gaaccttcag gagctcagct tgcttcactc accagccagg ctgccctttt catcacagat
     1621 cttcctgcga gatcgcctga aggtgatctg tgttaaattc gaggagctcc gagaggtacc
     1681 cctctgggtg tttgggcttc gaggcttaga agaactgcac ctggaaggac tctttccccc
     1741 agaaatggct cggggtgcca ccctggagag cctccgggag cttaaacagc tcaaggtact
     1801 gtctctacgt agcaatgcag ggaaggtgcc agccagtgtg actgatgtgg ccgggcacct
     1861 tcagcgactc agcctgcaca atgatggggc tcgtttgctt gccctaaaca gcctcaagaa
     1921 gctggcagtg ttacgggagc tggagctggt ggcctgtggg ctggaacgta tcccccatgc
     1981 aatcttcagc ctgggggctc tgcaggaact tgaccttaag gacaaccacc ttcggtctat
     2041 cgaggagatc ctcagcttcc agcactgtcg gaagctggtc actctcaggc tgtggcacaa
     2101 ccagattgcc tatgtgcccg agcatgtgag aaagctccgg agcctggagc agctctacct
     2161 cagtcacaac aagctggaga ctctgcccac ccagctgggc cagtgctttg gcctccgcct
     2221 gctggattta tcccacaacg ggcttcgttc cctgccacct gagttgggcc ttctccagag
     2281 cctccagcat ctagccctct cctacaacgc actcgagagt ctgcccgatg aattgttctt
     2341 ctgccacaag ctgcggacgt tactcttggg ctacaaccac ctaactcagc tctcacctga
     2401 cgtggctgcc ctccaggccc tcagccgtct ggagctcaag ggtaacaggt tggagacact
     2461 gccagaagaa cttggtgact gcaaggggct gaagaagtcg gggctgctgg tggaggacac
     2521 cctttatgag ggactgcctg cagaagttcg ggaaaagatg gaggaggaat gaagcctggt
     2581 gggggaaggg gcaggctgac acagaggcct ttacatgttt ctgcccaaca ttgtattcca
     2641 agggagagag gtccagcagg agtggaagga aggcaggaca tgtcggtttt gtgcagctca
     2701 gacaagatcc tgggactggt ttttctggag gagaaacagg aggcggtaga tttgaggtgt
     2761 aaccccttag ggaaggatta accagtctta ggttcttcaa ccaaaaccac acctgtgtct
     2821 ttggctggct ggcttgccct tcctctgaac attctggctt aattagtttc ctatatggga
     2881 gtgggatttc caacctttcc caacaaggat cagcatatac ttagggttct ctcctaagga
     2941 gaggatacag ggtccagttc caaagttcca tatcccaact ttcatgcaag tcctgatttt
     3001 ataagccctt tgtgacaggg atgggtactt agaacaatgg ctctcacatt tagctatgta
     3061 ataaaatcag ctgagaggcc cggaagaaaa caaaaccctt acatgcttcc caccccaccc
     3121 ctgaaattat actgactcag aatttggatg ggtagctgag catggtggca cacgccttta
     3181 attccagcac tcaggaggca gaggcaggtg gatttctgag ttcgaggcca gcctggtcta
     3241 caaagtgagt tccaggacag ccagggctac acagagaaac cctgtcaaaa aaaaaaaaaa
     3301 aaaaaattgg gtggggccag gaatctgcta ctaagtaaga atgccaggta attctgattc
     3361 aggtggtcct gagaccacat aatggaaata ttgacataaa gactgggctg gcactgaagc
     3421 tcagttgtga gagtgcttgc ttgacatgca ggcagccctg ggtcccgtcc tcagcatttc
     3481 ataatctagg agtggtggaa cctatgtaat gtagagatgc ctgtatagta ctctggaggt
     3541 ggagggagga ggcgtgaaag gttggaggta gcctgggaga cggagcatag ctcatctcaa
     3601 aacaactaag aatggaatac tgacatgtca gctatggagc cccttttctc gagccgtagg
     3661 gagtcccatc aagttaagca gtgttttctc aggacaatgc tcatggaaca taaagaagaa
     3721 actgtaagcc gagtctcctg tcacacattc ccacctgagt ccagtgctgg tgaaggctac
     3781 cttttttccc agttcattca gggattttct tttgaatgtt tcctgtgcag gggagcatat
     3841 accactgggt gcttaataaa cgagaacggt aatttcca
//
