LOCUS       NM_001122768            3944 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus leucine rich repeat containing 8D (Lrrc8d), transcript
            variant 1, mRNA.
ACCESSION   NM_001122768
VERSION     NM_001122768.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Lopez-Cayuqueo,K.I., Planells-Cases,R., Pietzke,M., Oliveras,A.,
            Kempa,S., Bachmann,S. and Jentsch,T.J.
  TITLE     Renal Deletion of LRRC8/VRAC Channels Induces Proximal Tubulopathy
  JOURNAL   J Am Soc Nephrol 33 (8), 1528-1545 (2022)
   PUBMED   35777784
REFERENCE   3  (bases 1 to 3944)
  AUTHORS   Butterfield,N.C., Curry,K.F., Steinberg,J., Dewhurst,H.,
            Komla-Ebri,D., Mannan,N.S., Adoum,A.T., Leitch,V.D., Logan,J.G.,
            Waung,J.A., Ghirardello,E., Southam,L., Youlten,S.E.,
            Wilkinson,J.M., McAninch,E.A., Vancollie,V.E., Kussy,F.,
            White,J.K., Lelliott,C.J., Adams,D.J., Jacques,R., Bianco,A.C.,
            Boyde,A., Zeggini,E., Croucher,P.I., Williams,G.R. and
            Bassett,J.H.D.
  TITLE     Accelerating functional gene discovery in osteoarthritis
  JOURNAL   Nat Commun 12 (1), 467 (2021)
   PUBMED   33473114
  REMARK    Erratum:[Nat Commun. 2021 May 28;12(1):3302. doi:
            10.1038/s41467-021-23768-8.. PMID: 34050183]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3944)
  AUTHORS   Zhou,C., Chen,X., Planells-Cases,R., Chu,J., Wang,L., Cao,L.,
            Li,Z., Lopez-Cayuqueo,K.I., Xie,Y., Ye,S., Wang,X., Ullrich,F.,
            Ma,S., Fang,Y., Zhang,X., Qian,Z., Liang,X., Cai,S.Q., Jiang,Z.,
            Zhou,D., Leng,Q., Xiao,T.S., Lan,K., Yang,J., Li,H., Peng,C.,
            Qiu,Z., Jentsch,T.J. and Xiao,H.
  TITLE     Transfer of cGAMP into Bystander Cells via LRRC8 Volume-Regulated
            Anion Channels Augments STING-Mediated Interferon Responses and
            Anti-viral Immunity
  JOURNAL   Immunity 52 (5), 767-781 (2020)
   PUBMED   32277911
REFERENCE   5  (bases 1 to 3944)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3944)
  AUTHORS   Stuhlmann,T., Planells-Cases,R. and Jentsch,T.J.
  TITLE     LRRC8/VRAC anion channels enhance beta-cell glucose sensing and
            insulin secretion
  JOURNAL   Nat Commun 9 (1), 1974 (2018)
   PUBMED   29773801
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3944)
  AUTHORS   Maruyama,T., Dougan,S.K., Truttmann,M.C., Bilate,A.M., Ingram,J.R.
            and Ploegh,H.L.
  TITLE     Increasing the efficiency of precise genome editing with
            CRISPR-Cas9 by inhibition of nonhomologous end joining
  JOURNAL   Nat Biotechnol 33 (5), 538-542 (2015)
   PUBMED   25798939
  REMARK    Erratum:[Nat Biotechnol. 2016 Feb;34(2):210. doi:
            10.1038/nbt0216-210c.. PMID: 26849523]
REFERENCE   8  (bases 1 to 3944)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   9  (bases 1 to 3944)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK137459.1 and BM934577.1.
            
            Transcript Variant: This variant (1) and variant 2 encode the same
            protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK137459.1, AK080722.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3905              AK137459.1         2-3906
            3906-3944           BM934577.1         515-553
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 50.68 cM"
     gene            1..3944
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /note="leucine rich repeat containing 8D"
                     /db_xref="GeneID:231549"
                     /db_xref="MGI:MGI:1922368"
     exon            1..286
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /inference="alignment:Splign:2.1.0"
     exon            287..431
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /inference="alignment:Splign:2.1.0"
     exon            432..3923
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /inference="alignment:Splign:2.1.0"
     CDS             434..3013
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /note="leucine rich repeat containing 5; leucine-rich
                     repeat-containing protein 8D; leucine-rich
                     repeat-containing protein 5"
                     /codon_start=1
                     /product="volume-regulated anion channel subunit LRRC8D"
                     /protein_id="NP_001116240.1"
                     /db_xref="CCDS:CCDS19494.1"
                     /db_xref="GeneID:231549"
                     /db_xref="MGI:MGI:1922368"
                     /translation="MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGT
                     MQLTKDQVVCLPVLPSPANSKAHTPPGNADITTEVPRMETATHQDQNGQTTTNDVAFG
                     TSAVTPDIPLQATHPHAESTLPNQEAKKEKRDPTGRKTNLDFQQYVFINQMCYHLALP
                     WYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETA
                     CEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTI
                     LDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAI
                     SFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIP
                     LKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREI
                     SLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPA
                     KISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLREL
                     YLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGT
                     KLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQH
                     LKRLTCLKLWHNKIVAIPPSITHVKNLESLYFSNNKLESLPTAVFSLQKLRCLDVSYN
                     NISTIPIEIGLLQNLQHLHITGNKVDILPKQLFKCVKLRTLNLGQNCIASLPEKISQL
                     TQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLPLEVKEALNQDVNVPFA
                     NGI"
     regulatory      3418..3423
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /note="hexamer: ATTAAA"
     polyA_site      3441
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /note="major polyA site"
     regulatory      3898..3903
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /note="hexamer: AATAAA"
     polyA_site      3918
                     /gene="Lrrc8d"
                     /gene_synonym="2810473G09Rik; 4930525N13Rik; A930019F03;
                     Lrrc5"
                     /note="major polyA site"
ORIGIN      
        1 gcggcgcctg cacgggcctg gagtccatgt tacgctttgt ctaattcccc tcgttacaga
       61 gtcatgtgct gctgctcccg tcgccgccgc tgccgccgcc cggggtgccc cggctcctcc
      121 gccgcgctta gaggtggcag ccgcgggcgg ggccgcggac tcagggtcca gggcgccgcg
      181 cgcctccggg gcgcgtcccg gtgcagccgc cgcccgccgc aggtgatgcc gccgcctccc
      241 gcctccgcct aagctcccgc acgcgcggcc cgggctgccc gcgaaggaag tgcactgctg
      301 actgcaacgt gctgccggct cccaggatgg aggagtgaag tctcgcgtcg cagcggttcc
      361 agcccccgga gcctgcccgc agccgagtcc tcgagagcgc cctgagggag cagggtggcc
      421 acctggtcca ggaatgttta cccttgcgga agttgcttca cttaatgaca ttcagccaac
      481 ttaccgaatc ctgaagccat ggtgggacgt gtttatggat tacctggcag tcgttatgct
      541 gatggtagcc atctttgcag ggaccatgca acttaccaaa gatcaggtgg tgtgtttgcc
      601 agtgttgccg tcgcctgcaa attcaaaagc gcacacacca cccggaaatg ctgacatcac
      661 caccgaagtc cccaggatgg aaacagccac acaccaagac caaaacgggc agacgacaac
      721 gaatgacgtt gcctttggca catccgctgt gacccctgac atacctctcc aagccaccca
      781 tcctcatgca gagtccaccc ttcccaatca ggaggcgaag aaggagaaga gagacccaac
      841 gggccgaaaa accaacttgg attttcagca gtacgtattt atcaatcaga tgtgttacca
      901 tctggccctc ccttggtact ccaagtactt tccatacctt gcccttatac acaccatcat
      961 ccttatggtc agtagcaact tttggttcaa atatcccaaa acgtgctcca aggttgagca
     1021 ttttgtttca atattaggaa agtgtttcga atctccctgg acaactaagg cgctgtcaga
     1081 gacggcctgt gaagactctg aggagaacaa gcagcggata acgggcgccc agaccctgcc
     1141 gaagcatgtg tccaccagca gcgatgaggg gagccccagc gccagcaccc ccatgatcaa
     1201 caaaactggc ttcaagttct cggctgagaa accggtgatc gaagttccca gcatgacaat
     1261 cctggacaag aaagacgggg agcaggccaa agccctgttt gagaaagtaa ggaaattccg
     1321 tgcccacgtg gaagacagtg acttgatcta taagctctat gtggtccaaa cgctcatcaa
     1381 aactgccaag ttcattttta tcctctgcta cactgcgaac tttgtcaacg ccatcagctt
     1441 cgagcacgtc tgcaagccaa aagtcgagca cctcacgggg tacgaggtgt tcgagtgcac
     1501 tcacaatatg gcctacatgt tgaaaaagct gctcatcagc tacatctcca tcatctgcgt
     1561 ctacggcttt atctgcctct acactctctt ctggctattc aggatccctc tgaaggagta
     1621 ctcgtttgag aaagtccggg aggagagcag cttcagcgac atcccggatg tcaagaacga
     1681 ctttgcgttc cttctgcaca tggtcgacca gtacgaccag ctctattcca agcgtttcgg
     1741 tgtgttctta tccgaagtca gtgagaacaa actgagggaa atcagcctga accatgagtg
     1801 gactttcgag aaactcaggc agcacgtgtc ccgcaatgcc caggataagc aggagctgca
     1861 cctcttcatg ctgtccgggg tgcccgacgc tgtctttgac ctcacagacc tggatgtgct
     1921 aaaacttgaa ctgatccccg aagcaaaaat tcctgccaag atctctcaga tgactaacct
     1981 ccaagagctc cacctctgcc actgccctgc aaaggtggaa cagactgcct ttagcttcct
     2041 ccgagatcac ttgagatgcc ttcacgtgaa gttcacggat gtggctgaaa tccccgcctg
     2101 ggtgtatctg cttaaaaacc tgcgggagct gtacttgatc ggcaatttga actcggaaaa
     2161 caataagatg atcggcctcg agtcgctgcg agagctgcgg caccttaaga tcctccacgt
     2221 gaagagtaat ctaaccaaag tcccctctaa catcaccgac gtggctccgc acctcacgaa
     2281 gctcgtcatc cacaacgacg ggaccaagct cttggtcctg aacagcctga agaagatgat
     2341 gaacgtggcc gagctcgagc tgcagaactg tgagctggag aggatcccac acgccatctt
     2401 cagcctctct aatctgcagg agctggattt aaagtcaaac aacatccgga cgatcgagga
     2461 gatcatcagc ttccaacatc tgaagcgact gacttgctta aagctgtggc acaataaaat
     2521 tgtggccatc ccaccctcca ttacccatgt caagaacctg gagtccctct acttctccaa
     2581 caacaagctc gagtctttac cgacggcagt gtttagttta cagaaactca gatgcttaga
     2641 cgtcagctat aacaacattt ccacgatccc catagagata ggtttgctcc agaacctgca
     2701 gcatttgcac atcacaggga acaaggtgga cattctgcca aaacagttgt ttaagtgcgt
     2761 gaagttgagg actttgaacc tggggcagaa ctgtatcgcc tccctgcctg agaaaatcag
     2821 tcagctcacc cagctcactc agctggagct gaagggcaac tgcctagacc gcctgccagc
     2881 ccagctgggc cagtgtcgga tgctcaagaa gagcgggctt gttgtagaag accaactgtt
     2941 tgacacgctg ccactagaag tcaaagaggc attgaatcaa gatgtcaatg tcccctttgc
     3001 aaacgggatt tgaactaagc gaatgcaggc acacccacgt gcggggggga caccttccta
     3061 gattgcagat gctcacttgc aagttattac aagataatgc attttaggag tagataagcc
     3121 ctttaaacag gacacgatgc atagaggctg gtagcagacg aaaccgaatg ttccatgttt
     3181 gtagcatttt cagtcattca tttccagatc ttttcttttt gtgggttgtt tttttttggg
     3241 gggggggtgt ttcctcttcc ttctcttctt cttcctcttc ctcctcctcc tcctcctcct
     3301 ttttctgggg aaagggaagg aaaaatcaca atcactgatc tcagttcttt ttaaattgta
     3361 agtcggatgc ggctgctggc tgagtgttta cacattgctt gcctgctaaa gtatatgatt
     3421 aaactgactt tcttcttact acattgcagt tttttggttt tttggttttt ttctttcttt
     3481 ctttcttgag gtgtcctgat ttatttttgc ttagctggtg caatattgct taaccttgga
     3541 tcgttgaaga cacaaggggc caaactcata atcttagatt tgctaaaata ttgacttctt
     3601 tactgtttta acttaaagca agtgagtgga gcttcctgga gcatttgatg gtactgtacc
     3661 tctgcgaatc ccacagcaga ctttacagtc ttggcggcct ggaatgaagt agagcgtgtg
     3721 gtgtgtcaag actctttgat tgtttcttaa aacaaatact aaaaataaaa taacacatcc
     3781 tttaatagtc actttatgtg cataggctgg agttttggtt atacatggta cacttttggg
     3841 ccaatataat aggatttcca atgaatagaa aatgtgtttt aaaaataaat ttataacaat
     3901 aaaatacgtt tatctaaatc gtaaaaaaaa aaaaaaaaaa aaaa
//
