LOCUS       NM_001033550            6690 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus leucine rich repeat containing 8 family, member B
            (Lrrc8b), mRNA.
ACCESSION   NM_001033550 XM_485656
VERSION     NM_001033550.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Lopez-Cayuqueo,K.I., Planells-Cases,R., Pietzke,M., Oliveras,A.,
            Kempa,S., Bachmann,S. and Jentsch,T.J.
  TITLE     Renal Deletion of LRRC8/VRAC Channels Induces Proximal Tubulopathy
  JOURNAL   J Am Soc Nephrol 33 (8), 1528-1545 (2022)
   PUBMED   35777784
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Zhou,C., Chen,X., Planells-Cases,R., Chu,J., Wang,L., Cao,L.,
            Li,Z., Lopez-Cayuqueo,K.I., Xie,Y., Ye,S., Wang,X., Ullrich,F.,
            Ma,S., Fang,Y., Zhang,X., Qian,Z., Liang,X., Cai,S.Q., Jiang,Z.,
            Zhou,D., Leng,Q., Xiao,T.S., Lan,K., Yang,J., Li,H., Peng,C.,
            Qiu,Z., Jentsch,T.J. and Xiao,H.
  TITLE     Transfer of cGAMP into Bystander Cells via LRRC8 Volume-Regulated
            Anion Channels Augments STING-Mediated Interferon Responses and
            Anti-viral Immunity
  JOURNAL   Immunity 52 (5), 767-781 (2020)
   PUBMED   32277911
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113980.29 and AC138265.9.
            
            On Oct 3, 2022 this sequence version replaced NM_001033550.3.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083378.1, SRR9219380.43934.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-97                AC113980.29        34225-34321         c
            98-152              AC138265.9         44267-44321
            153-251             AC138265.9         51656-51754
            252-2413            AC138265.9         55237-57398
            2414-6690           AC138265.9         61273-65549
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 50.68 cM"
     gene            1..6690
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /note="leucine rich repeat containing 8 family, member B"
                     /db_xref="GeneID:433926"
                     /db_xref="MGI:MGI:2141353"
     exon            1..97
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /inference="alignment:Splign:2.1.0"
     exon            98..152
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /inference="alignment:Splign:2.1.0"
     exon            153..251
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /inference="alignment:Splign:2.1.0"
     exon            252..2413
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /inference="alignment:Splign:2.1.0"
     CDS             275..2686
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /note="leucine-rich repeat-containing protein 8B; T-cell
                     activation leucine repeat-rich protein"
                     /codon_start=1
                     /product="volume-regulated anion channel subunit LRRC8B"
                     /protein_id="NP_001028722.1"
                     /db_xref="CCDS:CCDS39193.1"
                     /db_xref="GeneID:433926"
                     /db_xref="MGI:MGI:2141353"
                     /translation="MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGA
                     LQLTQSRVLCCLPCKVEFDNQCAVPWDLLKGSENASSNSGLLLPLPLRIQNDLHRQQY
                     SYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVSILHKCF
                     DSPWTTRALSETVAEQSVRPLKLSKSKTLLSTSGGSADIDASKQSLPYPQPGLESPGI
                     ESPTSSVLDKKEGEQAKAIFEKVKRFRLHVEQRDIIYRVYLKQIIVKVILFVLIITYV
                     PYFLSYITLEIDCSIDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVMLYGLTSSYSL
                     WWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVS
                     ENKLKQINLNNEWTVERLKSKLVKNSQDKVELHLFMLNGLPDNVFELTEMEVLSLELI
                     PEVKLPAAVAQLVNLRELHVYHSSLVVDHPALAFLEENLRILRLKFTEMGKIPRWVFH
                     LKNLKELYLSGCVLPEQLSSLHLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKL
                     SLDNEGSKLVVLNNLKKMVNLKSLELLSCDLERIPHSIFSLNNLHELDLKENNLKTVE
                     EIISFQHLPSLSCLKLWHNNIAYIPAQIGALSNLEQLFLGHNNIESLPLQLFLCTKLH
                     YLDLSYNHLTFIPEEIQYLTNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGRNSLTDL
                     SPLVGELSNLTHLELTGNYLETLPVELEGCQSLKRSCLIVEDSLLNSLPLPVAERLQT
                     CLDKC"
     exon            2414..6690
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6655..6660
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /note="hexamer: AATAAA"
     regulatory      6666..6671
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /note="hexamer: AATAAG"
     polyA_site      6676
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
                     /note="major polyA site"
     polyA_site      6690
                     /gene="Lrrc8b"
                     /gene_synonym="2210408K08Rik; mKIAA0231; Ta-lrrp"
ORIGIN      
        1 gcttcccgct gagccgcagc cgccggagtg cgggcgccgg ccgctcggct cgccccggcc
       61 ggccgggcag cgcgggcctg ggggctccga cacgaaggca ttcctcggat caaaactact
      121 tgcagagttt ccggttgacc agcattcata ggaatgaaga caaagtcaga gatggtgtgt
      181 ctgagaaact tcaaaaggtg tagacctccc gattgaagca tacctgattt ggttcatttt
      241 ccttcataat acctctcctc tctacaaggg agtcatgatt acactaacag agttaaaatg
      301 cttagcggac gcccagtcat cctaccacat actgaagccg tggtgggatg tcttctggta
      361 ttacatcacc ctcatcatgc tgttggtggc tgtgctggcc ggagccctgc agctcaccca
      421 gagcagggtt ctctgctgtc ttccctgcaa ggtggaattt gacaatcagt gcgctgtgcc
      481 ttgggacctc ctgaaaggca gcgagaacgc atcttctaac tcgggcctgc tgctcccgct
      541 gccactccgg atccagaacg acctccaccg gcagcagtac tcctacatcg atgctgtctg
      601 ctacgagaaa cagctccatt ggttcgccaa gtttttcccc tatctggtgc ttctgcacac
      661 cctcatcttc gcagcctgca gcaacttctg gcttcactac cccagcacca gctcgaggct
      721 ggaacacttt gtgtccatcc tccacaagtg cttcgactct ccgtggacca cacgcgccct
      781 gtcagagaca gtggcggaac agtcagtgag gcccctgaag ctctccaagt ccaaaacctt
      841 gctctcaacc tcagggggct ccgccgacat cgacgccagc aagcagtcat tgccctaccc
      901 acagccaggc ttggagtcgc ctggcataga aagcccaact tctagcgtcc tggacaagaa
      961 ggagggcgaa caagccaagg ccatctttga aaaagtgaag aggttccgcc tgcacgtgga
     1021 gcagagggat atcatctacc gggtgtacct gaagcagata atcgttaaag tcatcctgtt
     1081 tgtccttatt ataacctacg tcccctactt tctcagctac ataactcttg aaatcgactg
     1141 ttcgatcgac gtgcaggcat ttacaggcta taagcgctac cagtgtgtct actccttggc
     1201 agaaatcttc aaggtcctag cttcctttta cgtcattctg gtgatgcttt acggcctcac
     1261 gtcctcctac agcctgtggt ggatgctgag gagttccttg aagcagtact cctttgaggc
     1321 tctcagggag aaaagcaact acagcgacat ccccgatgtc aagaacgact ttgccttcat
     1381 cctccacctg gccgaccagt acgaccccct gtactctaag cgcttctcca tcttcctgtc
     1441 ggaggtcagc gagaacaaac tgaaacagat caacctcaac aacgagtgga ccgtggagag
     1501 actgaagagc aagctcgtga aaaactcgca ggacaaggtc gagctgcatc tcttcatgct
     1561 caacggtctc ccggataacg tctttgagct gacggagatg gaagtgctga gcctggagct
     1621 gatcccggaa gtcaagctgc cggctgccgt cgctcagctc gtcaacctca gggagctcca
     1681 cgtgtaccat tcgtccctgg tggtcgacca ccccgccctg gcctttctag aggagaactt
     1741 aagaatcctc cgcctgaaat ttaccgagat ggggaagatc ccacgctggg tgttccacct
     1801 gaagaacctc aaggagctgt acctgtcggg ctgcgttctc ccggagcagc tgagcagctt
     1861 gcacctggag ggcttccagg acttgaagaa cctgaggacc ctctacttga agagcagcct
     1921 gtcgcggatc cctcaggtgg tgacagacct gctgccctcc ctgcagaagc tgtcccttga
     1981 taatgaggga agcaaactgg ttgtgctgaa caacctgaag aagatggtca atctgaaaag
     2041 cctggagctg ctcagctgcg acctggagcg catcccccac tccattttca gcctgaacaa
     2101 cctgcacgag ctggacctga aggagaacaa cctcaagacc gtagaggaga tcatcagctt
     2161 ccagcacctc ccgagcctct cctgcctgaa gctctggcac aataacatcg cttacatccc
     2221 cgcgcagatc ggagcgctgt ctaacctgga gcagctcttc ctgggtcaca acaacatcga
     2281 gagtctccct ctgcagcttt ttctatgcac caaactacac tacttggatc taagctataa
     2341 ccatctgacc ttcattcccg aggaaatcca gtacctgacc aatctgcagt actttgctgt
     2401 gaccaacaat aacattgaga tgctaccaga cgggctgttc caatgcaaga agcttcagtg
     2461 tctactcctg ggaagaaata gcctaacaga tttgtcccct ctggtggggg agctgtcaaa
     2521 cctcacccac ctggaactca ctggtaacta cctggagaca ctgcctgtgg agctggaagg
     2581 gtgtcagtct ctgaaacgga gctgtctgat tgtcgaggac agtttgctca attccctccc
     2641 gcttcccgtg gcagaacgct tgcagacctg cttagataaa tgttaacctg aggttcagac
     2701 tcgaggcaaa agcagttcta aagaggcccc gggataggag gatgaacttt cctaagttat
     2761 caagatgaga gatggccggt gactctgaca cgccataacc aaaaatatta ccaaataacc
     2821 cagtgtccaa atcaagcagt taaatatcca caccggagtt ttgtgaataa ctgatgttta
     2881 aattattgag atgtaaaaag tatgcattga aggtggattg aggggcgtgt cgttacagaa
     2941 accttacctt tcacatctta tcttcagaac cctgtcccca cccccacccc aaccctttac
     3001 ttatttgcat acatgttttc atagaacctt gtagtctgat atttaactcc atgaagactc
     3061 gtcggtataa atctccttgt tgtatgctca ccactggctt tgatttatat tgtttctttt
     3121 ttttgagcag catgcatcac gctgggcaga attccatctt ggctgtcttc tctgctcctt
     3181 tctcattttg ctggaatcct catccaggct tctctgatag tcctgggaaa gtgcttacgt
     3241 ttggcgtttg cctagaacag gagccagggg ttctaggaca tgtgatcttc tgagcaccct
     3301 aaaaattaat gcattctcaa aatgtcttct ggtctatcat caaagccggt tttaaatatg
     3361 ttgaagtttt ttatatgaaa tataaaatat tttctactgt atgatctcag atgctacatc
     3421 tacgagaaca cctttttgta agcagtgaac atgtgcatct ctgtaagaat ttaatgattc
     3481 ttagtttggg ctgattatga ttctcatgtt tgtatgcatt ctgctccaaa aatttttgta
     3541 ctcttgcaac taattactgc aaccaatttg gatgacaaaa agccttgtgt tttgctccct
     3601 aacatctttg agggtttcct catgagagga caggagggcc gctgaatcac ctacctctcc
     3661 ttgctgttgt ccaagagtac gggtacaacc cctcaattac ttaaaaacta ccaccaaggg
     3721 gagagggcaa gatctagatt ggaaaattga ttggaaaata ctctgttaga tgtagccccc
     3781 ccccaatctg catgatctct caatatgact atgatagaag ccatctattc agaatctaac
     3841 tgagcccatg aaactgacat ctatctcata ggctgccttg tgacccctgg aatacgtcat
     3901 gttcctcctg acaaagagct ttgtgtttgg cccttttttt taatcatgcg tattgttgaa
     3961 taaataaaat agattgactt acactgggga agtggacatt gtagcatgtt tttctctcat
     4021 aagtctaggc attatgaaac cacctctttt tagacatagc tgtcaaataa tccattaact
     4081 gcatctaaaa aaaaaactgg aaaacacttc taaggagtct tctaaacaca gaactgtggc
     4141 atgttgtttt ctcctaattg ttctagaaag atgaaaatgt ctctcagtgg ccgtatatac
     4201 ctgatgtcta gagaagagaa tttcatggag gaaacctgag taaagttagt tacaaagaat
     4261 atactgaagg ccctaagctg cctaacacac tatggataga ctaaaagaga catcttagca
     4321 aagcttgcct ttgttagaat gtccaagttc tcagggacct aggcataact ggctctcact
     4381 ggggcctcat ggacaggccc tggcttagcc aggcttttct gatttttact gttttaataa
     4441 tgtgagctga tggctgacgt tttccagtaa aatcatcacg tggaccaatt tcaaagttga
     4501 ctggaattgt ctgaagtttt gaacataaat gtgagtgagg tttcaatact atcaatcgtg
     4561 cttcatatga actaaaaaaa atcccactct aaccagttca gactgtctaa gcctccccct
     4621 gatgaaggag aaatttcctg tgcctttgtt ttccagtata tgcatcattg gctaactggt
     4681 ctacctcctc aaagcagttc cctagatgga tcaatcatga ggtgtgagcc actttcaaca
     4741 cacaaggctg ttgttactgg tggaaaccat attgctcttg atacaacagt cttatagtgt
     4801 ctcttgtatt taacactgat gcatcaatgt tgaggtagct tttcaatcta acacaaagac
     4861 aatggacaac acatttccca cctgacagag atctgtgtta gcactggctt tactaaccta
     4921 agtgttgatc gccaccttac tgaagaaagt agtcttacaa attatcatga gattaaagtc
     4981 tcatggtaaa aatattcttt ccaaaaacca aatgtagagg ctcaaaaaca aaactttggc
     5041 ctatactaaa ataatatggg ctaatttgca tacacacata caagtacaga gaaagatgta
     5101 tagcttcagt tttgcaaatt tcagttttca atgtttttct gaaaattgca ggaggttata
     5161 ggttagtctg aggatagcaa cttttttaat tcattatttt taatcacgaa gtattcagtt
     5221 tctataaaat caactttagt ttaagaagat accatgtgtt cccacaaggg cagagttttc
     5281 ccctcatcta catcagggtg gatttctgca gcataattat actgaaattt tattttaacc
     5341 ttctgtgtgt tagcatattt ctgttaataa taaacaggtt gaatcagggt acgtgaaatt
     5401 gttgggttct cagtgataat ggcaagctct aatttgtgtg ctgcagtgtg ctaaagtggt
     5461 ttatgcacat gacattattc tttgatgatg aaatggttct gctcgtgttt cataattctg
     5521 ataccatgtt taattaaaac agcacatgat attgatgggt tcatgaaaat ggccaacact
     5581 tactctgtgg agttgtcact gtttccttac atttttcagg aaatccttcc atctctgcag
     5641 tcacgtggca ctggtccatc cgcacactgt tttgtcctca cccacctcac tgaatctgat
     5701 ggacactgta cagtcgcagt ctctttgtgc agaattggca gcgatgcctg tgcttgatca
     5761 tccctgttta taagaatcca gcttctggtc tctgttctca ttaaccgcat attgcattat
     5821 agaatgaaaa ataagagaga ctaattagag agaggaagga agacttattt tgcatcctga
     5881 atgaaaaaat atatatatat atatttagac agtgtaaaca aacaactgct gcagagtgtc
     5941 ctgtgtgtaa tagtgtctgg tgtgtacctg tgtgctgtgc taaatcaggg gaccctttct
     6001 gtctcttgtc catggtgtct atatccagtg acaagtgtct gctatgtacc ttttgcaatg
     6061 gacttcataa actgcattct ctaaaccttt tttttaaaca atttcatgaa caatttcata
     6121 aacactgaag tattaacaga ttatatgagt cagagtattg agctgagata gcttctaatt
     6181 gaacttaatt ttatatttaa gttttgaatt tatcacaaaa tacaccatag ctgagaaaaa
     6241 aacaattatg aatgccttct gcatgttcgt tatttctaac agacatggtg caatttttta
     6301 aatacagaaa ggtgtagggt tagaatggat gtgtctgcac tcatctctgt tgtctgtctg
     6361 tctttatatt cacactgtac agtaagactt aaagttctaa gtttcatgct aagtaaaagg
     6421 atgacccgat cttgggcgga gatacttagt tttgttagct cccagtggag gccggcattt
     6481 gcccttttac cttttaccat atacttggaa atgtgtatct ggaattaact actagaaaat
     6541 gtatgattat gttccatgtt tttggcccct ggattcaaat ccctacattt ggtagatttg
     6601 ggtaaatttt gctgttttta aagcatcttt tatgtaaaaa gattacattt ttataataaa
     6661 tggcaaataa gatgaatcac tagaatctga
//
