LOCUS       NM_177725               4254 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus leucine rich repeat containing 8A VRAC subunit A
            (Lrrc8a), mRNA.
ACCESSION   NM_177725
VERSION     NM_177725.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Wang,L., Cao,L., Li,Z., Shao,Z., Chen,X., Huang,Z., He,X.,
            Zheng,J., Liu,L., Jia,X.M. and Xiao,H.
  TITLE     ATP-elicited Cation Fluxes Promote Volume-regulated Anion Channel
            LRRC8/VRAC Transport cGAMP for Antitumor Immunity
  JOURNAL   J Immunol 213 (3), 347-361 (2024)
   PUBMED   38847616
  REMARK    GeneRIF: ATP-elicited Cation Fluxes Promote Volume-regulated Anion
            Channel LRRC8/VRAC Transport cGAMP for Antitumor Immunity.
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4254)
  AUTHORS   Wu,X., Yi,X., Zhao,B., Zhi,Y., Xu,Z., Cao,Y., Cao,X., Pang,J.,
            Yung,K.K.L., Zhang,S., Liu,S. and Zhou,P.
  TITLE     The volume regulated anion channel VRAC regulates NLRP3
            inflammasome by modulating itaconate efflux and mitochondria
            function
  JOURNAL   Pharmacol Res 198, 107016 (2023)
   PUBMED   38006980
REFERENCE   4  (bases 1 to 4254)
  AUTHORS   Wang,Y., Sun,Z., Ping,J., Tang,J., He,B., Chang,T., Zhou,Q.,
            Yuan,S., Tang,Z., Li,X., Lu,Y., He,R., He,X., Liu,Z., Yin,L. and
            Wu,N.
  TITLE     Cell volume controlled by LRRC8A-formed volume-regulated anion
            channels fine-tunes T cell activation and function
  JOURNAL   Nat Commun 14 (1), 7075 (2023)
   PUBMED   37925509
  REMARK    Erratum:[Nat Commun. 2024 Jan 5;15(1):309. doi:
            10.1038/s41467-023-44400-x.. PMID: 38182584]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4254)
  AUTHORS   Choi,H., Miller,M.R., Nguyen,H.N., Rohrbough,J.C., Koch,S.R.,
            Boatwright,N., Yarboro,M.T., Sah,R., McDonald,W.H., Reese,J.J.,
            Stark,R.J. and Lamb,F.S.
  TITLE     LRRC8A anion channels modulate vascular reactivity via association
            with myosin phosphatase rho interacting protein
  JOURNAL   FASEB J 37 (7), e23028 (2023)
   PUBMED   37310356
  REMARK    GeneRIF: LRRC8A anion channels modulate vascular reactivity via
            association with myosin phosphatase rho interacting protein.
REFERENCE   6  (bases 1 to 4254)
  AUTHORS   Sawada,A., Takihara,Y., Kim,J.Y., Matsuda-Hashii,Y., Tokimasa,S.,
            Fujisaki,H., Kubota,K., Endo,H., Onodera,T., Ohta,H., Ozono,K. and
            Hara,J.
  TITLE     A congenital mutation of the novel gene LRRC8 causes
            agammaglobulinemia in humans
  JOURNAL   J Clin Invest 112 (11), 1707-1713 (2003)
   PUBMED   14660746
REFERENCE   7  (bases 1 to 4254)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   8  (bases 1 to 4254)
  AUTHORS   Batias,C., Defamie,N., Lablack,A., Thepot,D., Fenichel,P.,
            Segretain,D. and Pointis,G.
  TITLE     Modified expression of testicular gap-junction connexin 43 during
            normal spermatogenic cycle and in altered spermatogenesis
  JOURNAL   Cell Tissue Res 298 (1), 113-121 (1999)
   PUBMED   10555545
REFERENCE   9  (bases 1 to 4254)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   10 (bases 1 to 4254)
  AUTHORS   Lalouette,A., Lablack,A., Guenet,J.L., Montagutelli,X. and
            Segretain,D.
  TITLE     Male sterility caused by sperm cell-specific structural
            abnormalities in ebouriffe, a new mutation of the house mouse
  JOURNAL   Biol Reprod 55 (2), 355-363 (1996)
   PUBMED   8828840
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ444045.1 and AL845258.5.
            
            On Apr 28, 2009 this sequence version replaced NM_177725.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927938.4309206.1,
                                           SRR7345562.4054321.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                BQ444045.1         48-59
            13-698              BQ444045.1         61-746
            699-2311            AL845258.5         142069-143681
            2312-4254           AL845258.5         148197-150139
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 21.41 cM"
     gene            1..4254
                     /gene="Lrrc8a"
                     /gene_synonym="ebo; Lrrc8; mKIAA1437; SWELL1"
                     /note="leucine rich repeat containing 8A VRAC subunit A"
                     /db_xref="GeneID:241296"
                     /db_xref="MGI:MGI:2652847"
     exon            1..37
                     /gene="Lrrc8a"
                     /gene_synonym="ebo; Lrrc8; mKIAA1437; SWELL1"
                     /inference="alignment:Splign:2.1.0"
     exon            38..146
                     /gene="Lrrc8a"
                     /gene_synonym="ebo; Lrrc8; mKIAA1437; SWELL1"
                     /inference="alignment:Splign:2.1.0"
     exon            147..2311
                     /gene="Lrrc8a"
                     /gene_synonym="ebo; Lrrc8; mKIAA1437; SWELL1"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..2587
                     /gene="Lrrc8a"
                     /gene_synonym="ebo; Lrrc8; mKIAA1437; SWELL1"
                     /note="leucine-rich repeat-containing 8; leucine-rich
                     repeat-containing protein 8A; protein ebouriffe; swelling
                     protein 1"
                     /codon_start=1
                     /product="volume-regulated anion channel subunit LRRC8A"
                     /protein_id="NP_808393.1"
                     /db_xref="CCDS:CCDS15875.1"
                     /db_xref="GeneID:241296"
                     /db_xref="MGI:MGI:2652847"
                     /translation="MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGT
                     LQVTQDKMICLPCKWVTKDSCNDSFRGWAASSPEPTYPNSTVLPTPDTGPTGIKYDLD
                     RHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSIL
                     LKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSR
                     IEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFA
                     LIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGL
                     ICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAV
                     FLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEV
                     LKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEI
                     PLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVG
                     VHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDN
                     NLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLF
                     YCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGN
                     NVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFSTLPPEV
                     KERLWRADKEQA"
     exon            2312..4254
                     /gene="Lrrc8a"
                     /gene_synonym="ebo; Lrrc8; mKIAA1437; SWELL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4235..4240
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrrc8a"
                     /gene_synonym="ebo; Lrrc8; mKIAA1437; SWELL1"
                     /note="hexamer: AATAAA"
     polyA_site      4254
                     /gene="Lrrc8a"
                     /gene_synonym="ebo; Lrrc8; mKIAA1437; SWELL1"
                     /note="major polyA site"
ORIGIN      
        1 cgggctgcct gcgggcggct gcgagcggag agcccagggc agcagcagcg tccatggagc
       61 aaaaggaatg tcagggccct gtgcatccag acagggacca gcctcagcac caacagccaa
      121 cacaaagata gcagagtcat ctcagggtta aaccatgatt ccggtgacag agctccgcta
      181 ctttgcggac acacagccag cataccggat cctgaagcct tggtgggatg tgttcactga
      241 ttacatctcc atcgtcatgc tgatgattgc tgtctttgga gggacgctgc aagtcaccca
      301 ggacaagatg atctgcctac cttgtaagtg ggtcaccaaa gactcctgca acgactcctt
      361 ccggggctgg gcagcctcca gcccagagcc cacttacccc aactccacag tcctgccgac
      421 gcctgacaca ggccccacag gtatcaagta tgacctagat cgacaccagt acaactacgt
      481 ggatgcggtg tgctacgaga accgcctgca ttggtttgcc aagtacttcc cctacctcgt
      541 gcttctgcac accctcatct tcctggcctg tagcaacttc tggttcaagt ttccacgcac
      601 cagttcgaag ctggaacact ttgtgtctat cctgctcaag tgcttcgact caccgtggac
      661 cacacgggcc ctgtcggaga cagtggtgga ggagagtgac cccaagccag ccttcagcaa
      721 gatgaacggg tccatggaca agaagtcatc tacagtcagc gaggatgtgg aggccaccgt
      781 gcccatgctg cagcggacca agtcacggat tgagcagggc atcgtggacc gatcggagac
      841 gggcgtactg gacaagaagg aaggggagca ggcgaaggcc ctgtttgaga aggtgaagaa
      901 gttccggact cacgtggagg agggggacat tgtgtaccgc ctgtacatgc ggcagaccat
      961 catcaaggtg atcaagttcg ccctcatcat ctgctacacc gtctactatg tgcacaacat
     1021 caagttcgac gtggactgca ccgtggacat cgagagcctg acaggctacc gcacctaccg
     1081 ctgtgcccac cccctggcca cgctcttcaa gatcttggca tccttctaca tcagcttggt
     1141 catcttctat ggcctcatct gcatgtacac actgtggtgg atgctgcggc gctccctcaa
     1201 gaagtactcg ttcgagtcga tccgagagga gagcagctac agtgacatcc ccgacgtcaa
     1261 gaacgacttt gccttcatgt tgcacctcat cgaccagtat gaccctctct actcaaagcg
     1321 cttcgccgtc ttcctgtctg aggtgagtga aaacaagctg cgccagctga acctcaacaa
     1381 cgagtggacg ctggacaagc tgcgccagcg cctcaccaag aacgcccagg acaagctgga
     1441 gctgcacctg ttcatgctca gcggcatccc ggacactgtg tttgacctgg ttgagctcga
     1501 ggtcctgaag ctggagctga tacccgacgt gaccatcccg cccagcatcg cccagctcac
     1561 aggcctcaag gagctgtggc tgtaccacac ggcagccaag atcgaggctc ctgccctggc
     1621 cttcctgcgg gagaacctgc gggccctgca catcaagttc acggacatca aggagatccc
     1681 gctgtggatc tacagcctga agacgctgga ggagctgcac ctgacgggca acctgagtgc
     1741 agagaacaac cgctacatcg tcattgacgg gctgcgggag ctcaaacgcc tcaaggtgct
     1801 gcgactcaag agcaacctga gcaagctgcc gcaggtggtc acggatgtgg gcgtgcacct
     1861 gcagaagctg tccatcaaca atgagggcac caagctcatt gtcctcaaca gcctcaagaa
     1921 gatggttaac ctgacggagc tagagctgat ccgctgtgac ctggagcgca tccctcactc
     1981 catctttagc ctccacaacc tgcaggagat tgacctgaag gacaacaacc tcaagaccat
     2041 tgaggagatc atcagcttcc agcacctgca ccgcctcacc tgccttaagc tgtggtacaa
     2101 ccacattgcc tacatcccca tccagatcgg caacctcacc aacctcgagc gcctctacct
     2161 gaaccgcaac aaaatcgaga aaatccccac ccagctcttc tactgccgca agctgcgcta
     2221 cctggacctc agccacaaca acctgacctt cctccccgcc gacattggcc tcctgcagaa
     2281 cctccagaac ctggccgtca cagccaatag gattgaagcg ctccccccag agctcttcca
     2341 gtgtcggaag ctacgggctc tacacctggg caacaatgta ctgcagtcgc tgccctcgag
     2401 agtgggcgag ctgaccaacc tgacgcagat cgagcttcgg ggcaaccggc tggaatgcct
     2461 gcccgtggag ctgggagagt gccccctact caagcgcagc ggcctggtgg tggaagagga
     2521 cttgttcagc acactgccac ctgaggtgaa ggagcggctc tggagagctg acaaggagca
     2581 ggcctgagca cagcctggag cagcagcaac gagcctccgc acaccctcag gccagatggg
     2641 cagcccagct ctcccaggac tgcgacagcc agctgggtcc agccagacca gaaagcaggg
     2701 actgaggggc tggtcccttt cctcccgctg ggacatggac acccaggaca ggcacttgct
     2761 ggagaccaga acctctgaga ctagtctcag agtgcagtat ttggacaatc agggtcttca
     2821 cactggaggc catctctgcc tcagaggccg agccagcacc agaggcccag gaacagtaac
     2881 ttagatctgg gtatttattt tttccacttc ccatcttttc cctccagata acttacacat
     2941 tcccaagaag gtccggctct gtgggaagtg tttagcaccc tggcagtgtt cacctttttc
     3001 tttttaggtg atgctgacag aatcaagtgc ccacccagac ttgggcagag ttgggtaggg
     3061 tgagccagcc gtaggacagt tgccttattg gcactgggct aggctctggc agcaaagctt
     3121 gtggaagacc aggcctccag gtggaagggt caggcctagg gcttgcccca tcaggttttg
     3181 aagcaatttt agattttttt tttaagtgag tttttttgtt tggttggttt tttttttttt
     3241 tttttttttt tttttttttt tttaagcttt gaaaatgatg gtttgggtat taaaaaaaat
     3301 taaaaaaaaa aaaaagacac taaggccagt gagttacagt ctcagggtgg gtggtgtttc
     3361 ccccttgagc aaagcagcaa gacattgaac agaatttcct gtctttgggg tgtgggccag
     3421 gatgtcttcc caatatgtcc tgaccgtggt ccagaagtgc cgtgctcttc tctcctgggg
     3481 caggctttta tttttgtttg cttttaaggt ggtttttttt ttgttgttgt tttttttttt
     3541 ggtgtcctgt ttttttgttg tttttttctc ctccatgggt cttggcaggc acccttttca
     3601 tggctgtcag ccaaaaggaa ggctctggag ctgccaagaa agggggagac ttaggctggc
     3661 taattcccag atgaacggtg ctccattccc ctccctaccc ccacaatgcc cctccacaca
     3721 cacagtgtta aggagcccag agcagctgct tcccctcccc caactctgtc cccacatcat
     3781 gggtttgctc agtgccaccc ctcacctctt ctgcttacat tagctctgtc accatctggt
     3841 ccatcgtgaa gagctgacat ttagaggcag attgggcatg agaggtcagc actgggaggg
     3901 caggctcccc agttttaggg ttccaggaca gtttgtactg ggcacctggt ttgcacacag
     3961 tacagtttag cgtctagtgg cggtaagcca ctctgcttta gatcaatcac tagggtcccc
     4021 acttagaagg gtcctcgcct tagatcaatc acatggacac taaggcacgt tttagcgtct
     4081 cttgtcttaa tgattatgtc tgtctctccg tccatttgtg ttttctgcgt cgtgtcatgg
     4141 atgtaaccct tggaaatact gcacactagc ctttgacaac catgaagcaa tccgttacac
     4201 gtgggtctga cttgtagact cagttcagat atcaaataaa tctataacac agtt
//
