LOCUS       NM_001401521           11407 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus leucine-rich repeats and guanylate kinase domain
            containing (Lrguk), transcript variant 1, mRNA.
ACCESSION   NM_001401521 XM_006506714
VERSION     NM_001401521.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   Okuda,H., DeBoer,K., O'Connor,A.E., Merriner,D.J., Jamsai,D. and
            O'Bryan,M.K.
  TITLE     LRGUK1 is part of a multiprotein complex required for manchette
            function and male fertility
  JOURNAL   FASEB J 31 (3), 1141-1152 (2017)
   PUBMED   28003339
REFERENCE   3  (bases 1 to 11407)
  AUTHORS   Liu,Y., DeBoer,K., de Kretser,D.M., O'Donnell,L., O'Connor,A.E.,
            Merriner,D.J., Okuda,H., Whittle,B., Jans,D.A., Efthymiadis,A.,
            McLachlan,R.I., Ormandy,C.J., Goodnow,C.C., Jamsai,D. and
            O'Bryan,M.K.
  TITLE     LRGUK-1 is required for basal body and manchette function during
            spermatogenesis and male fertility
  JOURNAL   PLoS Genet 11 (3), e1005090 (2015)
   PUBMED   25781171
  REMARK    GeneRIF: identified LRGUK-1 as a protein critically involved in
            multiple aspects of sperm assembly and function. LRGUK-1 is
            required for appropriate acrosome attachment to the sperm heard,
            sperm head shaping via the manchette and tail growth from the basal
            body; specific loss of LRGUK-1 function results in
            oligoasthenoteratospermia in mice.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153817.6.
            
            On Feb 15, 2022 this sequence version replaced XM_006506714.5.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164138,
                              SAMN01164141 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-325               AC153817.6         84736-85060
            326-433             AC153817.6         91808-91915
            434-515             AC153817.6         94615-94696
            516-616             AC153817.6         98712-98812
            617-698             AC153817.6         104166-104247
            699-823             AC153817.6         111378-111502
            824-967             AC153817.6         117658-117801
            968-1048            AC153817.6         126368-126448
            1049-1135           AC153817.6         127677-127763
            1136-1242           AC153817.6         129023-129129
            1243-1362           AC153817.6         133971-134090
            1363-1459           AC153817.6         141683-141779
            1460-1573           AC153817.6         145479-145592
            1574-1775           AC153817.6         148044-148245
            1776-1871           AC153817.6         150831-150926
            1872-11407          AC153817.6         156982-166517
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 14.75 cM"
     gene            1..11407
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /note="leucine-rich repeats and guanylate kinase domain
                     containing"
                     /db_xref="GeneID:74354"
                     /db_xref="MGI:MGI:1921604"
     exon            1..325
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..4051
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine-rich repeat and guanylate kinase domain-containing
                     protein"
                     /codon_start=1
                     /product="leucine-rich repeat and guanylate kinase
                     domain-containing protein isoform 1"
                     /protein_id="NP_001388450.1"
                     /db_xref="GeneID:74354"
                     /db_xref="MGI:MGI:1921604"
                     /translation="MAAFERNPSRWKGTRFRRGLGASRIAAQAILSLTEKQSQGRWPS
                     FPLGLKSKGTFRSASSYLLHQLIHRSHEAEAEQEEKQQEDGESEESEESEMQNLEDKY
                     DGILREETVAEAITGLGWSGRGTEQVYLNLNLSHCELVDISILCGYVHLQKLNLSGNR
                     IEDLSCVSCMPYLLELNASQNKLTTFFNFKPPQNLKKVDFSSNLISEMYDLSAYHTLT
                     QLILDNNEIEEITGLENCISLTHLSLAGNKITTIKGLGTLPIKVLSLSNNMIETITGL
                     EELKALQNLDLSHNQISSLQGLENHDLLEVINLEDNKIKELSEIEYIENLPILRVLNL
                     LRNPIQTKPEYWFFVIYMLLRLTELDQQKIKVEEKVFAVNKYDPPPEVVAVQDHMTHV
                     VNSMSQPQRIWDSTLPSLDAPYPMLILTGPAACGKRELAHRLCRQFSTYFRYGACHTT
                     RPPYFGEGDRVDYHFISQEVFDEMLNMGKFILTFNYGNHNYGLNRDTIEGIARDGLAS
                     CIHMELEGVRSLKYSYFEPRYILVVPMDKEKYEGYLRRKGLFSRAEIEIAVSRVDLYV
                     KVNQKYPGYFDAVINADDMDIAYQKLSELIREYLGLTETAAKTLAPTAGAPSSKKTAS
                     GVPAHLVPSPRRLARLQADGQKTEAFLEVQTQAVVPENQDPTLPQSQELTEEGEPPEK
                     ETNVPQQVSSSALGIPQQAQDLPPKVKEEDGQPANLPPKITTEMDGPEDEPGPPVPKA
                     DQSSTLASQEPPQQPAPAPTLSPQPAPAPTLSPQPAPAPTLSPQPAPAPTLSPQPDQD
                     KESGETKVAPSNPALSEPAQGADLASLSPQRVQDEGTESANPAPRSSTHTLPEDPSHT
                     EVEKPTGGSQRPLKEETPKAEVMRAGTPYPEIPPPQDSTTKVREQPGALLPRSRLAPT
                     RLPQPQTLAPLQSRRPTPKLLSPSREEALGTSSDQTPNPSPRSFPAQDGDPSKLPPIS
                     PSQSKPPRNSSPPTAHSPQQGQVGKASEVKLPLISPPVQEQAQQHTPNPPQEEEAPTV
                     QLPTIPAPSTEPQLPQNTEPRPASKPAREKKTPKVGRASSKKVLDLQATPHSQGPTKQ
                     KGAKKKNLMQKETAKESPQQRKMPVGNSQTAPQLESHDKPTPRNESDPLDFRSSPSHT
                     EPVPADPQNQEKNHKAHKPRKKAQTNPTPKDVAQSTHTSPNGEMSEGLPQGNETALGE
                     DQPTREGQPPQDPAKSAQEGSAPVLHPGEREQAQKREKSQKREVAGKPEGEEIAAPSQ
                     LRVKETQAHRDTRENRQSYAQRHSILVSKQQSKEKRTRKNGGVAQDRSPAAPQNQVSE
                     EDQGSRTGRLRARGSQSIKV"
     exon            326..433
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            434..515
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            516..616
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            617..698
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            699..823
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            824..967
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1048
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1135
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1242
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1362
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1459
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1573
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1775
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1871
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..11407
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5593..5598
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      5613
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /note="major polyA site"
     regulatory      10466..10471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /note="hexamer: AATAAA"
     polyA_site      10490
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /note="major polyA site"
     regulatory      10638..10643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /note="hexamer: AATAAA"
     polyA_site      10654
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
     regulatory      11383..11388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
                     /note="hexamer: AATAAA"
     polyA_site      11407
                     /gene="Lrguk"
                     /gene_synonym="4921528H16Rik"
ORIGIN      
        1 agtctcctag gcaacgcgta taaacaagat ggcggccttc gagcgaaatc cctcgagatg
       61 gaaaggcacc cgtttcaggc ggggtttggg cgcctctcgc attgcagccc aggcgatact
      121 gtcgctcaca gaaaaacaaa gtcagggccg ttggccttct ttccccctgg gtctgaagtc
      181 aaaagggact ttcagaagcg cctcctctta cctcctccac cagctcatcc accgctctca
      241 tgaggcagaa gcggagcaag aagagaagca acaggaggac ggagaatctg aagagtcaga
      301 ggagtccgaa atgcagaatt tggaggacaa gtatgacggg atcctgagag aggagaccgt
      361 ggctgaggca atcactggat tggggtggtc aggccgtggc acggagcagg tttacctcaa
      421 cctgaatcta tcgcactgtg agttggtcga catcagcatt ctgtgtggct atgtccacct
      481 gcagaagttg aatctttcgg gaaacagaat cgaagatttg tcttgtgtga gctgcatgcc
      541 gtatcttcta gaactaaatg cttctcagaa caagctgact accttcttca atttcaaacc
      601 accccaaaat ctcaagaagg tggatttttc ctccaacctg atttccgaga tgtatgacct
      661 gtcagcgtac catactctca ctcaactaat tttggacaac aacgagatag aagaaatcac
      721 tgggctagag aactgcatca gcctgaccca ccttagtctg gctggcaaca agatcactac
      781 catcaaaggc ttagggacat tacccatcaa agtgctttct ttgagtaaca acatgattga
      841 gaccatcaca ggtctggagg agctgaaagc cttgcagaac ctggacctgt cccacaatca
      901 gatcagcagc ctccagggct tggagaacca tgacctcctg gaagtgatta acctggagga
      961 taacaagatt aaggagctga gtgaaataga atacattgaa aatcttccta tccttcgagt
     1021 tctcaatcta ctaaggaatc caattcagac caagcccgag tactggttct tcgtgatata
     1081 tatgctgctg cgactgacag agttagacca gcagaagatt aaggtagaag aaaaggtttt
     1141 tgcagtgaat aagtatgacc ctccccctga ggtggtcgct gtccaagatc acatgaccca
     1201 tgttgtcaac agcatgtcgc agccacagag gatctgggac agcaccctgc cgagtctgga
     1261 tgccccgtat cccatgctca tactaactgg gcctgcagct tgcgggaaac gagaactcgc
     1321 ccaccgcctc tgcagacagt ttagtactta cttcagatat ggggcctgtc acaccacgag
     1381 accgccttac tttggtgaag gagatcgagt tgattatcat ttcatttccc aagaagtttt
     1441 tgacgaaatg ctgaacatgg ggaaatttat tttaacattt aattatggca atcacaatta
     1501 tggattaaat agggacacga tagaaggtat tgcaagagat ggattagcaa gctgcattca
     1561 tatggaatta gaaggtgtaa gaagtttgaa atattcttat tttgagcctc gatatatctt
     1621 ggtggtgcct atggacaagg aaaaatatga gggatacttg cggagaaagg gattgttcag
     1681 tcgcgcagaa atcgaaattg ccgtctccag ggtggacctt tatgttaaag ttaatcagaa
     1741 atacccagga tattttgatg cagtaatcaa tgcagatgac atggacattg cctaccaaaa
     1801 gctcagcgag ctcattagag aatacctggg gttgactgag acagctgcta agaccttggc
     1861 tccaactgca ggagctccct ctagcaagaa gaccgcctct ggggtccccg cacacctggt
     1921 cccctccccc aggcgcttgg caagactaca agccgatggc cagaaaacgg aggctttcct
     1981 ggaagtgcag actcaagcag tggtccctga aaatcaggac cccactctcc cacagagcca
     2041 ggagttaact gaggagggcg aaccccctga gaaggagact aatgtacctc agcaggtcag
     2101 ttcttcagca cttggtattc ctcagcaggc ccaggactta ccaccaaagg tgaaggaaga
     2161 ggatggccag ccagccaacc ttcctccaaa aataaccacc gagatggatg gcccagaaga
     2221 tgagcctgga ccgcccgtgc caaaagcaga ccagtcttcc actcttgcgt ctcaagagcc
     2281 gccccagcag cctgctcctg ctcccacact cagccctcag cctgctcctg ctcccacact
     2341 cagccctcag cctgctcctg ctcccacact cagccctcag cctgctcctg ctcccacgct
     2401 cagccctcag ccagaccagg ataaggaatc cggagagacc aaagtagccc ctagcaaccc
     2461 agccctatct gaacctgcac agggagctga cttagcttcc ctcagtcctc agagagtgca
     2521 ggatgaagga actgagtcag ccaaccctgc acccaggagt tccacgcaca ctcttccaga
     2581 agatccttcc cacactgagg tagagaagcc tactggggga tctcaacgac ctctgaagga
     2641 ggaaaccccc aaagcagaag ttatgcgggc cggtactcct tacccagaaa ttccacctcc
     2701 ccaagactca accacaaaag tcagggaaca gccaggagcc ttgcttccta gaagcaggct
     2761 ggctcctacc agattgccac agccccagac cttggctcct ctccagagcc ggagacccac
     2821 acccaagctt ctctctccca gcagggaaga ggctttgggg acaagctcag atcagacccc
     2881 gaatccctct cccagatctt ttccagctca ggatggagac cccagtaagc ttcctcccat
     2941 cagcccttcc caatccaaac caccccgaaa ctcgagcccc cctacagccc acagcccaca
     3001 gcaaggccag gtaggaaaag ccagtgaggt gaagctccca cttatcagcc ccccagtcca
     3061 ggaacaagcc cagcagcaca cccccaaccc accccaggaa gaggaggcac ccacagtcca
     3121 gctcccaact attcctgctc cctccacgga accccagctt cctcagaaca ccgagccaag
     3181 gcctgcttcg aagcccgcaa gggaaaagaa aactccgaaa gtaggccgtg cctccagtaa
     3241 gaaggttcta gatctgcagg ccacacccca cagccagggg cccactaagc agaaaggagc
     3301 gaagaaaaaa aacctcatgc aaaaagagac agctaaagag tcgccacagc agagaaagat
     3361 gccagttggg aactctcaga cagccccgca gctagaatcc cacgacaagc caactccaag
     3421 aaatgaatcc gacccacttg acttcaggtc ttctccgagc cacactgaac cagtgccagc
     3481 agacccccag aaccaggaga aaaaccacaa agcacacaag ccaagaaaga aggcacagac
     3541 caatcccacc cctaaggacg tggctcagtc cacacacaca tcccctaatg gagaaatgtc
     3601 tgaagggtta cctcaaggaa atgaaactgc cctcggtgag gatcagccaa ccagagaggg
     3661 tcagcccccg caggaccctg ccaagtctgc gcaggagggt tctgctcccg tgctgcatcc
     3721 tggggaaagg gaacaggctc aaaaaaggga aaagtctcag aagagagagg ttgcggggaa
     3781 gcccgaaggc gaggagattg cagcccctag ccaactgcgg gtcaaagaga cacaagccca
     3841 tagggacacc agggagaaca gacagagcta tgcccagagg cacagcatac ttgtatcgaa
     3901 gcagcaatcc aaagaaaaac gaacccgaaa aaatggaggt gtagcacagg acagaagtcc
     3961 tgctgctccc caaaatcagg tgtcagagga ggatcagggt agccggacag gaagactgcg
     4021 agccaggggc agtcagagta tcaaagtgtg agccttggct ccggagaagc tatgctgtca
     4081 gtggatgcaa gtccaacggc ttccttctgc agccactggt cagcaccttg atttgcgggg
     4141 tcccagaagt cgtcgtaatt ctgcttccca tgggacagac ttgggggaag acagctggct
     4201 ggttgctgcc agcgattcag tctagaacac ttgatgactg gtgtcatcaa aaggtcctcg
     4261 tgttcgacta caaatgtcaa tgggatttca tggcggaacc caggcagatt gccatcttcc
     4321 ttaggaaaat tcacttctag attagaaatt ttcctcgtgc tttttacatt attggaggtg
     4381 gggtggggct gcagcaaaag gaactctgca aaaagaaacc ttgctgtttt taaggattgc
     4441 caacctgcag ttcacttgtg agagtgcatc tctgcaagcc agacaatggc cgagccaagt
     4501 gtttgaggtc tgcattggtc tatgttatga aaattccttt tgtttctaca tttttccaaa
     4561 tgaatacctt ccttccgtct tcaaatagga atctcatggt ccatgccttc ctcatgccac
     4621 agcatctgat tgaatcccaa gaaatctgtg gggagaaaat tcaggggaat cagtcttcat
     4681 ttatctctcc atattattct cttctattcc tgtcaatgac taaggtgcag ccagggctag
     4741 cctgtggaaa gctattagca ggaagtatgt gtacatctca tataaccaaa ctaccagaag
     4801 gacacaggta ggtgggggct ctcagagaaa caaggggtca gctattgagc atcaccaggg
     4861 gtctcgttat cttctggacc aagcaagagc ctaggaccca catcaattct catttctgtt
     4921 gttctccatg ttggctccat tctttgaact acagatggtt caaagccatt ctctctacat
     4981 agcagggaca tgaacacgaa caactctgca attacatccg gtggcatctc ttacctttgt
     5041 tataaagaca ttaatctaaa tcatcattaa tcctaatatt taaaagaaaa tccaaggaaa
     5101 gaccgattgc ctctttgatc aaacattcat ttccaaccaa caactctggc ctgggcatgg
     5161 ctaccaccac ccatagccca ctgggtccac tgctctagat ggagacttac atttccagaa
     5221 ggaaggataa ttgcagtcaa gacatcagcc ctcaattcca ctctttaaaa aaaaaatgtt
     5281 agaagataac catgtataca ggaatgttct gaagctatgt ttactgtccc gtatttcatc
     5341 atgtagagaa tgactagagc ctgtgtcttg tcttatacaa aagctatgtg cccacaagcc
     5401 tgtatgacta gcaaatgtta tgtcaagtgc aatagaggac atcgatattt tttaaatcgg
     5461 ggatgtaacg gagcctctca gcgaacaagg ctgttcttgt aactataata ttagacgtta
     5521 tcttctgatt tatctccctt ctccaagata aaaagctata ggatgatttt tgaaagtaat
     5581 ttatgtttat taagtaaatt cattctcaag taagcctttg tggagcactt tctctgagcc
     5641 agacatagtg atggtagcta ggtgtggagt aagacgatgc tgacaaagac gtgtctgaca
     5701 aaggtccatt caattaggtc catgtaatta gggtacccgg gagccccaaa gcatgctgag
     5761 atttatgtgg caccccatag ataagaaaac ctgttggtct gcttttctag attgtagtaa
     5821 gagtgctctc tgagaaggcc acagagttca gcctactaga gctgtcccag gaaactccaa
     5881 ggaaaagcca atgcctcagc tgaacctgca gggctgtaga attggttgcg gagagatggg
     5941 gtaggggatg cttcctgttg cctaaataca caggcaggag cagctagtgt ctccgaagga
     6001 cccagacgcc aggtgaccta taggcctatc taacccgtgc cataagaagg agcttgggaa
     6061 gccacatctt gtagcagcaa agggagggct ggatgcttca atgtgtactt cagagataaa
     6121 gttaagaaaa acaatcctgg aggcagaatg actggtacat catggtggga gtcaggacag
     6181 ggcaaggcaa gggcctgaaa gatggcactg gtgtcaggga aggccaaatg ctcatttggc
     6241 ttagctgtgg agggggtgga ttatataaga cagcaagtga ggggttgcct ccatttctct
     6301 ttccctccct ccctcccttc tttccttttt tttgagaggg tgtcactgtg tagcccttcc
     6361 tgggctagag cttgctatat agaccaggtg gactttgaac tcagagagac ccacctgtgt
     6421 ctgcttcctg agtgttggga ttaaaggggt gcgccccgat gtaccactca ttttctggat
     6481 atggcatttg ggatgctgta aggaaccaga gcctcacctg gttagcaggc ttcagaatca
     6541 catcaggtgt cctctttggc tgccgtttgg aggcccttgt tgctgccttt ttcattcatg
     6601 ttggagtgga cacactaagc aagtgtctct ctgctgtaag gaggctctag gccctggtat
     6661 tcataataat aagaaaggtt tgggtttttt tttcctttgt gcttctttta tttctgtggt
     6721 ttgaagttgt atgcctcaga ctcactgctg ttggacattt tcagaggagt gggaaagact
     6781 gagggggttt tgcggctagt attgattacc ttgctacaag gaaaaaatgt aattttcaat
     6841 gcagagaata aatatttaaa atgatcattt ctccatctgc accaaccggc aaagttcaca
     6901 tcgcctccaa ctgggtttgt cacaaaatga tctttagaga ggaaaagaaa agccagggtt
     6961 ggctttaaaa gtcactgtac tcgtaatgac accttcttag ggaagtctgg catttagtgt
     7021 tcaaatgatg agggcattcc caccaggtat aagagccttc tgcacacatg gaaagagctg
     7081 agctcccaca agacagagaa ggcacaatca tagctcacaa aataagggag taaaggttgt
     7141 cccataagtg tgtcagaaac aatgtggcct cgttgtaaat taaaacagaa cagactgttt
     7201 tgatagatag caggctggga catataaaac aggttagggt ctgtccgggt gaggaagttg
     7261 atgagcatgc ttttctgaag aacagttttc cactctctgt caaagagtta cagtttgcac
     7321 acttttagtc tgatcatcta gatccaaggg ttaccatgaa ggaatgtaca cgagaatgtt
     7381 catatcaaat tttataatga aggttagaaa tactctatgt aatagaattt ttgaaataca
     7441 tttacaacat agacatctgc cttatgttgc cgttataacc atgtagaata tttatttgaa
     7501 aaacagtgtt cattacatat gagggagaaa gagggcaaag aaggtacagt caacttggct
     7561 cttgtgttag ctccaaaata ctaagttgtg gcaatttttt tctctcattc atgtttttgg
     7621 tactgtctaa attttctgcc atgtctattt agtatatttc aatttgatat aataaggaac
     7681 tttctaagag tccaaggtga cctgcagaga aacagaatgg cctcctccgg gcgtcagatg
     7741 tatcacctta cgaaaaagct tctatctggg gtgattttat agtatggatg gagcctggtt
     7801 ttctttatga tctatgatcc acttagaaca actggtctac ttgttggtta tgaagaatcc
     7861 agatgccttt ttcattaagt agagtgtttt tgtagtagaa aatgaaatgt tcttagtctc
     7921 cattagctcc ctggcctaaa gaatgttttt ggagcaagtt ccatagatat aagtatattt
     7981 cacagtatca caacccagta aacacaacta cataaaggaa ataaagtatt ctaagacacc
     8041 cttcataggc gatggactcg tattttctat ggtgttgagt ttaaattttc taaatgcagt
     8101 aggttgtggc ctactaaatc aatttcacag tgtagtctgc acattgtagt ctttcacgct
     8161 gtagtccagt tcctggggcc tgcttgccag gtctcctgct ttctctgtct cccttcaggt
     8221 tggctggata gtcttgcctc acctcatact gagttctcgg ggagcaggag ctggctttct
     8281 ggcagttata ccttttgtta ttgttggcat cagcctgaat atatccaagc ttcctataca
     8341 tagctataag ctcttatata aagcttacaa aacacttcaa aacattcgtt atcaatggtg
     8401 atgctaccac taaatagtca gtccccatgc cctctgcgcc atgacaaccc tataaacaac
     8461 actgttacag taaaggagcc tccgagttcc tcttctggtc actgagctgt tgatacggta
     8521 tccagcacag tttacccata agtgaatcta cgtgtgtggt agggtttgaa atagtggttt
     8581 tgtgttgact aagtatccag agaagagagc aaatgcctgc ccctctttct gtgtgctgat
     8641 agctaatgtt agctgatagc taatgcagac cgctaggctt aaggcctact gtggtgacaa
     8701 cacgtgatat cagtcatgag catcaaaatg atgctaatga ttgtaacctt agaaactctc
     8761 aattacaaat gacactgaat gtcaaaacca tccctggaaa cttgaggcac agtacaatac
     8821 agtcaggagt gagggaggga gtgtactgaa agcgcccaga acaacatctg ctacatggtt
     8881 agtactgaga tgagcgttcg ttagcactaa cacgtaaaca gcaaaagaat attacattcg
     8941 atcatgaatt agaacaggac aatgtatcct gtttaagtgg aagtgtttgt taagtgaaac
     9001 aatcttccct gaaaatccct ttatgacttc agccttgtct aaattaaaga gcttagacaa
     9061 aactaggatg atactagaaa aatgatactt cagtataata ttcctaagat agagggcttc
     9121 ttcagtgggc attcacaggc aacctgccac tgcagaagac agggatgggg gcatcaaatt
     9181 ggagccaatg gtgaatctcc ttgtggtata cacaccaaac acttaggata acagctggcc
     9241 tatgtagtac acgctcaata aatgctatct gttcttttta ttgttacctt gttgatgata
     9301 tcaaatatat tttactgtat agagagcaaa tgcttgcctc ctcaccatta cgtgtctggg
     9361 taatagctgt tttgttttct ctttgtggtg gcttttactc ctggatctac gaaccctcag
     9421 tttccaagaa caaagctaaa catccaagtg gagaaaggca gaaaagcctt tgctggggaa
     9481 agcgagcgat aaaatagtgc ctaagagaaa attgttccta aagctgagtt gggccaggcg
     9541 cagcagtatg accctggctc ccagctactc aagaggctga ggaaagaggg tcccttaaat
     9601 caagggaggt tagcaacaag gcattccctt tcagatgcgg cagtgtgggg catcagaaat
     9661 gtttactcag atatattggt caaaggaaag gaaactttag gcctgcatta aagctccctg
     9721 tgggtttctg tcccctcctg cctgtcactg ctagctcccc ctgactccag tttcccatat
     9781 actacagatc ttataaacca accttcccct cttgacattt gccctgccgc cccatagttt
     9841 atttccttca aagcatccac taacaacttt atgataaact tactcatttt gtaaaacgtt
     9901 ctgtcattat cgtacatttt aattggaaca ttctggattc agtatctgaa tgctggcttg
     9961 ttggttgaac gccattgaag acattacaaa atataaagtc tggaagaaag aaaaggtttt
    10021 gaacttcatt ttctctgtat atgaatatat tttcgcatgc gtctttttcc tggtgtggag
    10081 tctgtttctg tttaaatagc tagctatccc tttgattgga caccgaagaa tagagcacct
    10141 cactgttcgc atattttagc aaatgtattc attttgccat tgcaacatga acattattac
    10201 tctatttcct tggatttatg agattgcttt ctttgtacat aggatcttat tttgctatgg
    10261 aaaattatca tgggcattat atgaaatgat ttttctactt cttattattc ctttcttagt
    10321 acacaaatag acatgatgtt ttttgagatg caattctgcc ttggaaaaat agcttctttt
    10381 ggtaccttca gttttgtgga aatactccct aatgaaccca atgtttttca tttcccacca
    10441 caaattgtgt agcagttcta ttagaaataa atactttaca ttctatagca cattattatg
    10501 ttttaatttg tcatgagtca ttttcaagct tgtatatttc atgatgtata tttctatgca
    10561 tatttcttgt aaagggttgt gggagtttac ttgtgtgtat gtattaatcg tggcaggggt
    10621 aaattactct tagttaaaat aaaataaaac tgcatgtttt tagagccttc tttgcatggg
    10681 aggaagaaaa tgaatacttt tgaagtcgtt gggtactgca aatttttttt ttctttctga
    10741 aaactggagc ctatttccca agtcatccat ttccagagac tgttcctttg gaagagagaa
    10801 ctggttaacg tacaactcct tttcacactt ttatttagct attcacagag tctcctttgg
    10861 aagaatgacg agcctcgtgt aagtcgtctg actaccagag aaacactggt taataagctg
    10921 cagcgttcac tggtcatatt attcacagcc tggttgccac gaggcaattg aattgtcctt
    10981 actgacctgc gtgattgcct gtgcgacccc atccttaaca aataggccgg tttctcctgc
    11041 cctgtgtgtg aactacccta ggtagcctgt gtggtgcact ggtaagcatg cacgcacatg
    11101 atttgttcct agatggtttg tctctactgt atgtgatgat cagactggat tctgtgctta
    11161 ttcctttaag atgcattgtg caaatagtat ttaagtcgtt taacttcaaa ggaatgtgtc
    11221 tacatacaag gtgtaagtca tttaagataa tgtggaacta cattccagtt atttccatat
    11281 ctttctgcat tgccctgcat ctggtaatga tcctatccat aaagcaactt taaaatccac
    11341 cacgctccat taaacgcatt tacgcttcac cgtaggcaat taaataaatt taactggcct
    11401 atataaa
//
