LOCUS       NM_146164               3081 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus leucine-rich repeats and calponin homology (CH) domain
            containing 4 (Lrch4), transcript variant 1, mRNA.
ACCESSION   NM_146164
VERSION     NM_146164.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Aloor,J.J., Azzam,K.M., Guardiola,J.J., Gowdy,K.M.,
            Madenspacher,J.H., Gabor,K.A., Mueller,G.A., Lin,W.C., Lowe,J.M.,
            Gruzdev,A., Henderson,M.W., Draper,D.W., Merrick,B.A. and
            Fessler,M.B.
  TITLE     Leucine-rich repeats and calponin homology containing 4 (Lrch4)
            regulates the innate immune response
  JOURNAL   J Biol Chem 294 (6), 1997-2008 (2019)
   PUBMED   30523158
  REMARK    GeneRIF: identify Lrch4 as a broad-spanning regulator of the innate
            immune response
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   3  (bases 1 to 3081)
  AUTHORS   Shiio,Y., Rose,D.W., Aur,R., Donohoe,S., Aebersold,R. and
            Eisenman,R.N.
  TITLE     Identification and characterization of SAP25, a novel component of
            the mSin3 corepressor complex
  JOURNAL   Mol Cell Biol 26 (4), 1386-1397 (2006)
   PUBMED   16449650
  REMARK    GeneRIF: SAP25 is a novel core component of the Sin3 corepressor
            complex whose subcellular location is regulated by PML.
REFERENCE   4  (bases 1 to 3081)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   5  (bases 1 to 3081)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 3081)
  AUTHORS   Rougeon,F., Chambraud,B., Foote,S., Panthier,J.J., Nageotte,R. and
            Corvol,P.
  TITLE     Molecular cloning of a mouse submaxillary gland renin cDNA fragment
  JOURNAL   Proc Natl Acad Sci U S A 78 (10), 6367-6371 (1981)
   PUBMED   6171818
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY204138.1, CN702489.1, CF729493.1, DV661712.1, BQ924416.1,
            DV650548.1, CV783140.1 and AC124730.5.
            
            On Dec 12, 2009 this sequence version replaced NM_146164.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC012525.1, SRR7652917.222836.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                BY204138.1         1-71
            72-726              CN702489.1         1-655
            727-1221            CF729493.1         242-736
            1222-1349           DV661712.1         649-776
            1350-1850           BQ924416.1         104-604
            1851-1884           DV650548.1         402-435
            1885-2320           CV783140.1         1-436
            2321-3081           AC124730.5         101451-102211
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 76.59 cM"
     gene            1..3081
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /note="leucine-rich repeats and calponin homology (CH)
                     domain containing 4"
                     /db_xref="GeneID:231798"
                     /db_xref="MGI:MGI:1917193"
     exon            1..304
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..2127
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /note="isoform 1 is encoded by transcript variant 1;
                     leucine-rich repeat and calponin homology
                     domain-containing protein 4; leucine rich repeat protein
                     4, neuronal"
                     /codon_start=1
                     /product="leucine-rich repeat and calponin homology
                     domain-containing protein 4 isoform 1"
                     /protein_id="NP_666276.1"
                     /db_xref="CCDS:CCDS19772.1"
                     /db_xref="GeneID:231798"
                     /db_xref="MGI:MGI:1917193"
                     /translation="MAAAVAGPLAAGGEEAAASVSLPGSPGLPGSRSAERALEEAVAT
                     GTLNLSNRRLKHFPRGAARSYDLSDITQADLSRNRFPEVPEAACQLVSLEGLSLYHNC
                     LKCLNPALGNLTALTYLNLSRNQLSSLPPYICQLPLRVLIISNNKLGALPPDISTLGS
                     LRQLDVSSNELQSLPVELCSLRSLRDLNVRRNQLSTLPDELGDLPLVRLDFSCNRISR
                     IPVSFCRLRHLQVVLLDSNPLQSPPAQICLKGKLHIFKYLTMEAGRRGAALGDLVPSR
                     PPSFSPCPAEDLFPGRRYDGGLDSGFHSVDSGSKRWSGNESTDDFSELSFRISELARD
                     PRGPRQPREDGAGDGDLEQIDFIDSHVPGEDEDRSAAEEQLPSELSLVAGDVEKPSSS
                     RREEPAGEERRRPDTLQLWQERERKQQQQSGGWGSPRKDSVLKRGIRAAGAGASAPST
                     QATCNGPPKSSTTQLGVSGGQGAPTPPPTSQDPLPVSGPVTAPVPRPLGSIQRPNSFL
                     FRSSSQSGSSPSSPESVLRPRPFPQEKELISQLRQVLESRLQQPLPEDLAEALANGVL
                     LCQLANQLRPRSVPFIHVPSPAVPKLSALKSRKNVESFLEACRKMGVPEADLCSPSDL
                     LRGTAQGLQTVLEAVILVGGKAPLPVQPSSGLGGFLLFYVVFMLLLYVVYTRLLGS"
     exon            305..449
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            450..576
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            577..682
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            683..819
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            820..932
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1032
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1123
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1200
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1262
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1379
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1448
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1552
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1636
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1713
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1851
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..1929
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..3081
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2464..2469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /note="hexamer: TATAAA"
     polyA_site      2484
                     /gene="Lrch4"
                     /gene_synonym="2810008P14Rik; 2900069C24Rik; LRN; LRRN4;
                     SAP25"
                     /note="major polyA site"
ORIGIN      
        1 ggactacggc ggctccgagg agggggaagg gcgagaaggg ccggccggtg ccgtcagtca
       61 ggcaggggga gccgccgaga gcggatggcg gcagcagtag cgggcccact cgccgccggg
      121 ggtgaggaag ctgcagcttc agtgtccttg ccagggtctc ctggtctacc tgggagccgt
      181 agcgcagaac gagccctaga ggaggctgtg gccaccggga ccctgaactt gtccaaccgg
      241 cgtttgaagc acttcccccg gggcgcggcc cgcagttacg acttgtcaga catcacccag
      301 gctgacttgt ctcggaaccg gtttcccgag gtgcctgagg cagcttgcca gctggtgtcc
      361 ctggaaggcc tgagcctcta ccacaattgc ctgaaatgcc tgaacccagc cttggggaat
      421 cttacagccc tcacctacct caacctcagc cggaaccagc tgtcgtcgtt gccaccctac
      481 atctgccagc tgccccttcg agtgcttatc atcagcaaca acaagttagg agccctgcct
      541 ccagacatca gcaccttggg aagcctgcgg cagcttgatg tgagcagcaa tgagctgcag
      601 tccctgcccg tggagctgtg tagcctccgt tccctgcggg atctcaatgt tcgaaggaac
      661 cagctcagta ccctgcctga tgagctggga gaccttcctc tggtccgcct ggatttctcc
      721 tgtaaccgca tctcccgaat ccccgtctcc ttctgccgcc tcaggcacct gcaggtcgtt
      781 ctgctggata gcaaccccct acagagtcca cctgcccaga tatgcctgaa ggggaaactt
      841 cacatcttca agtacctaac aatggaagct ggccggaggg gagccgccct cggggacctg
      901 gtcccttccc gccccccaag ttttagtcct tgccctgccg aagatttatt tccgggacgt
      961 cgttatgatg gtggcctgga ctcaggcttc cacagcgttg acagtggcag caagaggtgg
     1021 tcaggaaatg agtccacaga tgatttttca gagctgtctt tccggatctc ggagctggct
     1081 cgtgatcccc gggggcctag acaacctagg gaagatggcg ctggcgatgg agacctggag
     1141 cagattgact ttattgacag ccacgttcct ggggaagatg aagatcgaag tgcagctgag
     1201 gagcagctgc cttctgaatt aagccttgta gcaggggatg tggagaagcc atctagcagc
     1261 aggcgagagg agcctgcagg ggaggagagg cggcgcccag acactttgca gttgtggcag
     1321 gaacgggagc ggaagcaaca gcaacagagt gggggatggg ggtcccccag gaaggacagc
     1381 gtcctgaagc gggggatccg agctgccggg gcaggtgctt cggccccatc cacacaggcc
     1441 acctgcaatg gcccaccaaa gtccagcact acccaactgg gagtttcagg ggggcaggga
     1501 gctcccacac caccccccac ctcccaggac ccccttcctg tatctggacc agtgacagct
     1561 cctgttccca ggcccctggg ctccattcag agaccaaaca gcttcctctt ccggtcctct
     1621 tctcagagtg gctctagtcc ttcctctcca gagtctgttt tgagacctcg gccgtttcct
     1681 caggagaagg agctaatatc ccaacttcgc caggtcctag agtcgcggct gcagcagccc
     1741 ctacctgagg acctggcaga agctctcgcc aacggagtcc tcctttgcca gctggcgaac
     1801 cagctgcggc cccgctctgt acccttcatt catgtgccct cacctgctgt gccaaagctc
     1861 agtgctctca agtctcggaa gaatgtcgag agtttcctag aagcctgtcg gaaaatgggt
     1921 gtgcctgagg ctgacctgtg ctcgccctcg gatctcctcc ggggcaccgc ccaggggctg
     1981 cagaccgtcc tggaggctgt gatcctggtt gggggcaagg cccctctccc agtccagccc
     2041 tcctctggtc tgggtggctt cctcctcttc tacgtggtct tcatgctgct gctctatgtc
     2101 gtctacactc ggctcctggg ctcctaggac ccacgttgcc ccttccttcc ccaccttcta
     2161 tttataagac ctcctgttca accggtggtg ccttcagccc taccaaagac actagtgtcc
     2221 cctcaccaac gctgacctca gaagccccac tctcataccc acaataccct tacccttagt
     2281 gctgatggtg ccttcgtgtt ctcccggcca gccctctgtc ccccgtcgga tgacaggact
     2341 gtggcttttc tcttctgtcc tccctccccc taccagctgc tatgggggag ctaaattatc
     2401 tctgttttgt agagagaact attagcaggg gatgtgagcc cagtatgggc ccctggctct
     2461 gtgtataaac tgtatagatt gtcacctctg tgtgtgcgtg tgtgagctcc tgctgcagcc
     2521 aggtcttgtc ctgcctgggc tacctgcctg tgcaagcctt tccctttgga aatgacaggc
     2581 ctctcctgtc ccagtcccct gtgttaacca cccgtctgtt acctgctggc agcttctatg
     2641 tgtcatttgt ccccaggtat ggggcagtgt ctaccaggag ttcccaccct cttaaccttg
     2701 ctgggggaat tcgaggggga aacctaacct gggcccctcc ccccaggagt ccctgtgcca
     2761 gccccaccac atcctggaag aggaaggggc ccctggaagg ggcctccctc acatcgctgc
     2821 tgtccttcac gcacttctgg aacagcctta ggggtagagg ttgggggctc tgggccccaa
     2881 ctaccatcgg acctgtcctg ggagcagatg acacctgggc ccagccaatt aacaaggact
     2941 tggtgaccat ggtcttgtgt gtagcactgg ccttggatat ccccctgccc ttaggctgct
     3001 tcgggtacag cccccatctg ggtgaagagc gttgtgtcaa acacaataaa aagaagccca
     3061 aaggcctgta ggttggtcct c
//
