LOCUS       NM_134152               1708 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus leupaxin (Lpxn), transcript variant 1, mRNA.
ACCESSION   NM_134152
VERSION     NM_134152.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Butterfield,N.C., Curry,K.F., Steinberg,J., Dewhurst,H.,
            Komla-Ebri,D., Mannan,N.S., Adoum,A.T., Leitch,V.D., Logan,J.G.,
            Waung,J.A., Ghirardello,E., Southam,L., Youlten,S.E.,
            Wilkinson,J.M., McAninch,E.A., Vancollie,V.E., Kussy,F.,
            White,J.K., Lelliott,C.J., Adams,D.J., Jacques,R., Bianco,A.C.,
            Boyde,A., Zeggini,E., Croucher,P.I., Williams,G.R. and
            Bassett,J.H.D.
  TITLE     Accelerating functional gene discovery in osteoarthritis
  JOURNAL   Nat Commun 12 (1), 467 (2021)
   PUBMED   33473114
  REMARK    Erratum:[Nat Commun. 2021 May 28;12(1):3302. doi:
            10.1038/s41467-021-23768-8.. PMID: 34050183]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1708)
  AUTHORS   Bonaud,A., Clare,S., Bisio,V., Sowerby,J.M., Yao,S., Ostergaard,H.,
            Balabanian,K., Smith,K.G.C. and Espeli,M.
  TITLE     Leupaxin Expression Is Dispensable for B Cell Immune Responses
  JOURNAL   Front Immunol 11, 466 (2020)
   PUBMED   32269569
  REMARK    GeneRIF: Leupaxin Expression Is Dispensable for B Cell Immune
            Responses.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1708)
  AUTHORS   Klapproth,S., Bromberger,T., Turk,C., Kruger,M. and Moser,M.
  TITLE     A kindlin-3-leupaxin-paxillin signaling pathway regulates podosome
            stability
  JOURNAL   J Cell Biol 218 (10), 3436-3454 (2019)
   PUBMED   31537712
  REMARK    GeneRIF: The kindlin-3 not only activates and clusters integrins
            into podosomes but also regulates their lifetime by recruiting
            leupaxin, which controls PTP-PEST activity and thereby paxillin
            phosphorylation and downstream signaling.
REFERENCE   5  (bases 1 to 1708)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1708)
  AUTHORS   Chew,V. and Lam,K.P.
  TITLE     Leupaxin negatively regulates B cell receptor signaling
  JOURNAL   J Biol Chem 282 (37), 27181-27191 (2007)
   PUBMED   17640867
  REMARK    GeneRIF: Leupaxin plays an inhibitory role in BCR signaling and B
            cell function.
REFERENCE   7  (bases 1 to 1708)
  AUTHORS   Huntington,N.D., Tabarias,H., Fairfax,K., Brady,J., Hayakawa,Y.,
            Degli-Esposti,M.A., Smyth,M.J., Tarlinton,D.M. and Nutt,S.L.
  TITLE     NK cell maturation and peripheral homeostasis is associated with
            KLRG1 up-regulation
  JOURNAL   J Immunol 178 (8), 4764-4770 (2007)
   PUBMED   17404256
REFERENCE   8  (bases 1 to 1708)
  AUTHORS   Sahu,S.N., Khadeer,M.A., Robertson,B.W., Nunez,S.M., Bai,G. and
            Gupta,A.
  TITLE     Association of leupaxin with Src in osteoclasts
  JOURNAL   Am J Physiol Cell Physiol 292 (1), C581-C590 (2007)
   PUBMED   16914530
  REMARK    GeneRIF: Association of the scaffold protein LPXN with Src adds
            further complexity to the organization of the podosomal signaling
            complex in osteoclasts.
REFERENCE   9  (bases 1 to 1708)
  AUTHORS   Watanabe,N., Amano,N., Ishizuka,H. and Mashima,K.
  TITLE     Leupaxin binds to PEST domain tyrosine phosphatase PEP
  JOURNAL   Mol Cell Biochem 269 (1-2), 13-17 (2005)
   PUBMED   15786712
REFERENCE   10 (bases 1 to 1708)
  AUTHORS   Gupta,A., Lee,B.S., Khadeer,M.A., Tang,Z., Chellaiah,M.,
            Abu-Amer,Y., Goldknopf,J. and Hruska,K.A.
  TITLE     Leupaxin is a critical adaptor protein in the adhesion zone of the
            osteoclast
  JOURNAL   J Bone Miner Res 18 (4), 669-685 (2003)
   PUBMED   12674328
  REMARK    GeneRIF: leupaxin may be a critical nucleating component of the
            osteoclast podosomal signaling complex
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC150901.2 and AC135633.3.
            
            On Mar 31, 2018 this sequence version replaced NM_134152.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026563.1, AB071194.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               AC150901.2         200552-200652
            102-259             AC150901.2         205906-206063
            260-306             AC150901.2         213193-213239
            307-406             AC135633.3         143495-143594       c
            407-574             AC135633.3         138819-138986       c
            575-748             AC135633.3         137958-138131       c
            749-830             AC135633.3         137759-137840       c
            831-979             AC135633.3         130174-130322       c
            980-1708            AC135633.3         129117-129845       c
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 8.73 cM"
     gene            1..1708
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /note="leupaxin"
                     /db_xref="GeneID:107321"
                     /db_xref="MGI:MGI:2147677"
     exon            1..101
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..1249
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="leupaxin isoform 1"
                     /protein_id="NP_598913.1"
                     /db_xref="CCDS:CCDS29638.1"
                     /db_xref="GeneID:107321"
                     /db_xref="MGI:MGI:2147677"
                     /translation="MEELDALLEELERCTFQDSEEYSNPVSCHLDQQSTEESKIPQTP
                     KTLSSQGNTSPLKVQLVYATNIQEPNVYSEVQEPKESVLPPKTSAAAQLDELMAHLSE
                     MQAKVSVKADTSRKPLPDQQDHKASLDSMLGDLEQELQDLGIATVPKGYCASCQKPIA
                     GKVIHALGQSWHPEHFVCTHCKEELGSSPFFERSGLAYCSKDYHRLFSPRCAYCAAPI
                     TDKVLTAMNKTWHPEHFFCSHCGEVFGAEGFHEKDKKPYCRKDFLAMFSPKCGGCNRP
                     VLENYLSAMNTVWHPECFVCGDCFSSFSSGSFFELDGRPFCELHYHHRRGTLCHDCGQ
                     PITGRCISAMGHKFHPEHFVCAFCLTQLPKGIFKEQNNKTYCEKCFTKLFSQ"
     exon            102..259
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            260..306
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            307..406
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            407..574
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            575..748
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            749..830
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            831..979
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1708
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1678..1683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /note="hexamer: AATAAA"
     polyA_site      1703
                     /gene="Lpxn"
                     /gene_synonym="4933402K05Rik; A530083L21Rik; LDPL"
                     /note="major polyA site"
ORIGIN      
        1 attcagttcc gttgtggtcc tcttcttcac cacatgccaa caaagttctt cacaatcttc
       61 gagactagag catcgcaggt ataggacaat ggaagagctg gatgccttat tggaggaatt
      121 ggaacgctgc acctttcagg acagtgagga atattcaaat ccagtttctt gtcacctgga
      181 tcagcaatcc acagaggaga gcaagattcc ccaaactcca aagaccttgt catcgcaggg
      241 taacacaagt cccttgaagg tgcagctcgt gtatgcaacc aatatccagg agcccaatgt
      301 ctacagtgag gtccaagagc caaaggaatc agtcctgcct cccaaaacct cagcagctgc
      361 tcagttggat gagctcatgg cccacctgag tgaaatgcag gccaaggttt cagtgaaagc
      421 agacaccagc aggaaaccct tgccagacca gcaggatcac aaggcatctc tggactcaat
      481 gctgggggac ttggaacagg aactacaaga tcttgggatt gccacagtcc ccaagggcta
      541 ctgtgcttcc tgccagaagc caattgctgg gaaggtgatc catgctctgg ggcaatcctg
      601 gcacccagag cactttgtct gcactcactg caaggaggag ctcggctcca gtcccttctt
      661 tgagcggagt ggcctggcct actgctcaaa agactaccac cgcctgttct ctccacgctg
      721 tgcctactgt gctgctccca tcacagataa agtgctgaca gcaatgaaca agacatggca
      781 cccagagcac ttcttctgct ctcactgtgg agaggtattt ggtgcagaag gctttcatga
      841 gaaggacaag aagccatact gccgaaagga tttcttagcc atgttctcac ccaaatgtgg
      901 tggctgcaac cgcccagtgc tggaaaacta cctttcagcc atgaacactg tctggcaccc
      961 agagtgcttt gtgtgtgggg actgcttcag tagtttttct tctggctcct tctttgaact
     1021 ggatggccgt cctttctgtg aactccatta ccatcaccgc cgagggaccc tctgccatga
     1081 ctgtgggcag cccatcactg gccgttgcat cagtgccatg ggacataaat ttcatcctga
     1141 gcacttcgtg tgtgctttct gcctgacaca gctgccgaaa ggcatcttca aggagcagaa
     1201 caacaagacc tactgtgaaa aatgcttcac taagctcttt tcacagtagt tctcctttga
     1261 ctcacatctg cttcatgttg cctataaaac tgaggccaag ataggaaaga gcatagattc
     1321 tgtccccagc cttctgttca gtgggctaat ggagtacaaa cgggcctttg tagatgttac
     1381 taggcatata ccttcatttt gaagatgctt ctcattactt tttaattata tatttagatt
     1441 tagatcatgc tatcaagagc ctctgaaggt atatttctta tgtgcacagt tctttccact
     1501 gctggttttc tctttctcac ctttgacctc tgctgatgct ccattcaaat cttcagatgt
     1561 gggccacagg gttttactct ctctttgctg tcaggggtca cgcaatagtc ttaaaccctt
     1621 agtgtctctg cattttgctt tttaagcgga agtgtatttt tactgacaca tgtgatgaat
     1681 aaagcagctt gtggttttgg tgactcac
//
