LOCUS       NM_001357791            5768 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus lipin 2 (Lpin2), transcript variant 3, mRNA.
ACCESSION   NM_001357791 XM_006524787
VERSION     NM_001357791.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Zhang,X., Huang,S., Zhuang,Z., Han,X., Xie,M., Yu,S., Hua,M.,
            Liang,Z., Meng,C., Yin,L., Zhuang,X. and Chen,S.
  TITLE     Lipin2 ameliorates diabetic encephalopathy via suppressing
            JNK/ERK-mediated NLRP3 inflammasome overactivation
  JOURNAL   Int Immunopharmacol 118, 109930 (2023)
   PUBMED   37001383
  REMARK    GeneRIF: Lipin2 ameliorates diabetic encephalopathy via suppressing
            JNK/ERK-mediated NLRP3 inflammasome overactivation.
REFERENCE   3  (bases 1 to 5768)
  AUTHORS   Watahiki,A., Hoshikawa,S., Chiba,M., Egusa,H., Fukumoto,S. and
            Inuzuka,H.
  TITLE     Deficiency of Lipin2 Results in Enhanced NF-kappaB Signaling and
            Osteoclast Formation in RAW-D Murine Macrophages
  JOURNAL   Int J Mol Sci 22 (6), 2893 (2021)
   PUBMED   33809261
  REMARK    GeneRIF: Deficiency of Lipin2 Results in Enhanced NF-kappaB
            Signaling and Osteoclast Formation in RAW-D Murine Macrophages.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5768)
  AUTHORS   Watahiki,A., Shimizu,K., Hoshikawa,S., Chiba,M., Kitamura,H.,
            Egusa,H., Fukumoto,S. and Inuzuka,H.
  TITLE     Lipin-2 degradation elicits a proinflammatory gene signature in
            macrophages
  JOURNAL   Biochem Biophys Res Commun 524 (2), 477-483 (2020)
   PUBMED   32008742
  REMARK    GeneRIF: Lipin-2 degradation elicits a proinflammatory gene
            signature in macrophages.
REFERENCE   5  (bases 1 to 5768)
  AUTHORS   Zhang,P., Csaki,L.S., Ronquillo,E., Baufeld,L.J., Lin,J.Y.,
            Gutierrez,A., Dwyer,J.R., Brindley,D.N., Fong,L.G., Tontonoz,P.,
            Young,S.G. and Reue,K.
  TITLE     Lipin 2/3 phosphatidic acid phosphatases maintain phospholipid
            homeostasis to regulate chylomicron synthesis
  JOURNAL   J Clin Invest 129 (1), 281-295 (2019)
   PUBMED   30507612
  REMARK    GeneRIF: These data implicate lipin 2/3 as a control point for
            enterocyte phospholipid homeostasis and chylomicron biogenesis.
REFERENCE   6  (bases 1 to 5768)
  AUTHORS   Donkor,J., Zhang,P., Wong,S., O'Loughlin,L., Dewald,J., Kok,B.P.,
            Brindley,D.N. and Reue,K.
  TITLE     A conserved serine residue is required for the phosphatidate
            phosphatase activity but not the transcriptional coactivator
            functions of lipin-1 and lipin-2
  JOURNAL   J Biol Chem 284 (43), 29968-29978 (2009)
   PUBMED   19717560
  REMARK    GeneRIF: A conserved serine residue is required for the
            phosphatidate phosphatase activity but not the transcriptional
            coactivator functions of lipin-1 and lipin-2.
REFERENCE   7  (bases 1 to 5768)
  AUTHORS   Gropler,M.C., Harris,T.E., Hall,A.M., Wolins,N.E., Gross,R.W.,
            Han,X., Chen,Z. and Finck,B.N.
  TITLE     Lipin 2 is a liver-enriched phosphatidate phosphohydrolase enzyme
            that is dynamically regulated by fasting and obesity in mice
  JOURNAL   J Biol Chem 284 (11), 6763-6772 (2009)
   PUBMED   19136718
  REMARK    GeneRIF: lipin 2 plays an important role as a hepatic PAP-1 enzyme.
REFERENCE   8  (bases 1 to 5768)
  AUTHORS   Grimsey,N., Han,G.S., O'Hara,L., Rochford,J.J., Carman,G.M. and
            Siniossoglou,S.
  TITLE     Temporal and spatial regulation of the phosphatidate phosphatases
            lipin 1 and 2
  JOURNAL   J Biol Chem 283 (43), 29166-29174 (2008)
   PUBMED   18694939
  REMARK    GeneRIF: distinct and non-redundant functions of lipin 1 and 2
            regulate lipid production during the cell cycle and adipocyte
            differentiation
REFERENCE   9  (bases 1 to 5768)
  AUTHORS   Donkor,J., Sariahmetoglu,M., Dewald,J., Brindley,D.N. and Reue,K.
  TITLE     Three mammalian lipins act as phosphatidate phosphatases with
            distinct tissue expression patterns
  JOURNAL   J Biol Chem 282 (6), 3450-3457 (2007)
   PUBMED   17158099
  REMARK    GeneRIF: Lipin-2 exhibits phosphatidate phosphatase type-1 (PAP1)
            activity, which has a key role in glycerolipid synthesis.
REFERENCE   10 (bases 1 to 5768)
  AUTHORS   Peterfy,M., Phan,J., Xu,P. and Reue,K.
  TITLE     Lipodystrophy in the fld mouse results from mutation of a new gene
            encoding a nuclear protein, lipin
  JOURNAL   Nat Genet 27 (1), 121-124 (2001)
   PUBMED   11138012
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154218.2, AC126942.4 and AW489885.1.
            
            On Nov 10, 2017 this sequence version replaced XM_006524787.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.63697.1,
                                           SRR9219381.180046.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-213               AC154218.2         23493-23705         c
            214-414             AC126942.4         33856-34056
            415-510             AC126942.4         40947-41042
            511-812             AC126942.4         43832-44133
            813-920             AC126942.4         48200-48307
            921-1044            AC126942.4         49164-49287
            1045-1381           AC126942.4         50075-50411
            1382-1481           AC126942.4         52720-52819
            1482-1669           AC126942.4         56278-56465
            1670-1763           AC126942.4         57500-57593
            1764-1833           AC126942.4         57976-58045
            1834-1923           AC126942.4         59704-59793
            1924-2006           AC126942.4         60536-60618
            2007-2151           AC126942.4         61518-61662
            2152-2300           AC126942.4         62180-62328
            2301-2387           AC126942.4         62817-62903
            2388-2540           AC126942.4         63664-63816
            2541-2655           AC126942.4         64011-64125
            2656-2759           AC126942.4         65308-65411
            2760-5727           AC126942.4         65719-68686
            5728-5768           AW489885.1         1-41                c
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 41.87 cM"
     gene            1..5768
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /note="lipin 2"
                     /db_xref="GeneID:64898"
                     /db_xref="MGI:MGI:1891341"
     exon            1..213
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            214..414
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..2904
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /EC_number="3.1.3.4"
                     /note="isoform 2 is encoded by transcript variant 3;
                     phosphatidate phosphatase LPIN2"
                     /codon_start=1
                     /product="phosphatidate phosphatase LPIN2 isoform 2"
                     /protein_id="NP_001344720.1"
                     /db_xref="CCDS:CCDS28956.1"
                     /db_xref="GeneID:64898"
                     /db_xref="MGI:MGI:1891341"
                     /translation="MNYVGQLAGQVLVTVKELYKGINQATLSGCIDVVVVRQQDGSYQ
                     CSPFHVRFGKLGVLRSKEKVIDIEINGSAVDLHMKLGDNGEAFFVEETEEEYEKLPAY
                     LATSPIPTEDQFFKHIETPLVKSSGNERPAQSSDVSHTLESEAVFTQSSVKKKKRRRK
                     KCKQDNRKEEQAASPVAEDVGDVGVSSDDEKRAQAARGSSNASLKEEDYKEPSLFHSG
                     DNYPLSDGDWSPLETTYPQAVCPKSDSELEVKPSESLLRSEPHMEWTWGGFPESTKVT
                     KRERYDYHPRTATITPSENTHFRVIPSEDSLIREVEKDATVEDTTCTIVKPKPRALCK
                     QLSDAASTELPESPLEAPQISSLLDADPVPSPSAEAPSEPKPAAKDSPTKKKGVHKRS
                     QHQGPDDIYLDDLKALEPEVAALYFPKSDTDPGSRQWPESDTFSGSQSPQSVGSAAAD
                     SGTECLSDSAMDLPDVTLSLCGGLSENGEISKEKFMEHIITYHEFAENPGLIDNPNLV
                     IRIYNRYYNWALAAPMILSLQVFQKSLPKATVESWVKDKMPKKSGRWWFWRKKESMIK
                     QLPETKEGKSEVPPANDLPSNAEEPTSARPAENDTSSDEGSQELEESIKVDPITVETL
                     SHCGTASYKKSLRLSSDQIAKLKLHDGPNDVVFSITTQYQGTCRCAGTIYLWNWNDKV
                     IISDIDGTITKSDALGQILPQLGKDWTHQGIARLYHSINENGYKFLYCSARAIGMADM
                     TRGYLHWVNDKGTILPRGPLMLSPSSLFSAFHREVIEKKPEKFKIECLNDIKNLFAPS
                     RQPFYAAFGNRPNDVYAYTQVGVPDCRIFTVNPKGELIQERTKGNKSSYHRLSELVEH
                     VFPLLSKEQNSAFPCPEFSSFCYWRDPIPDLDLDDLA"
     exon            415..510
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            511..812
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            813..920
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1044
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1381
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1481
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1669
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1763
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1833
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1923
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2006
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2151
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2152..2300
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2387
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2540
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..2655
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..2759
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..5751
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5731..5736
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5751
                     /gene="Lpin2"
                     /gene_synonym="2610511G02Rik"
                     /note="major polyA site"
ORIGIN      
        1 gggccggggc ggggcgtgcg cgcagtctac cgccagctcg gccgccgccg ccgctgctgc
       61 cgccgccgcg tcccattcat gaggcccggc gggctccgct gtagcccgcg gcttcggagg
      121 cggcggcccc gccccctgct cccccgcttc ttggggcatc cctgcggggc tgcacaacag
      181 tccgccgagt gagagccgcg cgcggtgccg cagtctcaaa ccatgaatta tgtgggccag
      241 ctggctgggc aggtgctcgt cactgtgaag gaactctaca agggcattaa ccaagccacg
      301 ttgtctggat gcatcgatgt ggtcgtggtg aggcagcagg atggctccta ccagtgctcg
      361 ccttttcacg tacgcttcgg gaagctgggt gtcctgaggt ccaaggagaa agtgattgac
      421 atagaaatca atggcagtgc tgtggatctt cacatgaaat tgggtgataa tggggaagcc
      481 ttctttgtag aggagactga agaagaatat gaaaaattac ctgcttatct tgccacctca
      541 ccaattccca ctgaagacca gttctttaaa catattgaaa cccctttggt gaaatcaagt
      601 ggaaatgaaa ggccagctca gagttcagac gtttctcaca ccttggaatc agaggcagtt
      661 ttcactcaga gttctgtgaa aaagaagaaa cgaaggagaa agaagtgcaa acaggacaat
      721 aggaaggagg agcaggcagc ttcccctgtt gcagaagatg taggtgatgt gggtgtgagc
      781 tcagatgatg agaagagagc ccaggcagca agaggatctt caaatgcttc cttaaaggaa
      841 gaggactaca aggagccttc actcttccat tctggggata actacccctt atctgatgga
      901 gattggtccc cattagaaac cacctaccct caggctgtgt gccccaagag tgactctgag
      961 ctggaggtga agccatctga gagcctcctc agatctgagc cgcacatgga gtggacgtgg
     1021 ggcgggttcc cagagtccac caaggtcacc aaaagagaac ggtatgacta tcatccaagg
     1081 acagctacga ttacaccatc agagaacaca catttcaggg taattcccag tgaagacagc
     1141 ctcataagag aagttgaaaa ggatgctact gttgaagata ctacctgtac catagtgaaa
     1201 cccaaaccta gagccctgtg taagcaactg agtgatgcag cgtctactga gcttcccgaa
     1261 tcacctcttg aagcacctca gatttcatcg ttattagatg cagaccctgt tcccagccca
     1321 tcagcagagg ctccctcaga acccaaacca gctgctaaag actcaccaac aaaaaagaaa
     1381 ggtgttcaca aaagaagcca gcaccaggga cctgatgaca tttaccttga tgacttaaag
     1441 gctcttgagc ctgaagtggc ggctctctat ttccctaaaa gtgacacgga tccaggttcc
     1501 aggcagtggc ctgagtctga cacattctct ggttctcagt ccccacagtc tgtggggagt
     1561 gcagctgcgg acagtggcac tgaatgcctc tcagactctg ccatggactt gcctgatgta
     1621 accctctccc tctgtggagg cctcagtgag aatggagaga tttctaaaga gaagttcatg
     1681 gagcatatca tcacttacca tgagtttgca gaaaaccctg gccttatcga caacccaaac
     1741 ctcgtgatcc ggatatataa ccgttactac aactgggcgt tggctgctcc catgatcctt
     1801 agcttacagg tatttcagaa gagtttgcct aaggccaccg ttgagtcctg ggttaaagac
     1861 aagatgccaa agaaatctgg tcgatggtgg ttttggcgga aaaaagaaag tatgatcaaa
     1921 cagttgccag agaccaagga gggaaaatct gaggtccctc cagcaaatga cctgccttcc
     1981 aatgctgagg agccaaccag tgccagacct gcagagaatg acacttctag tgacgagggg
     2041 tcacaggagt tggaagaaag catcaaagtt gaccccatca ccgtagagac actgagtcac
     2101 tgtgggacgg cctcatataa gaagtctctc cgactctcct cggaccagat agcaaaactg
     2161 aagctccatg atggccccaa tgacgtggta ttcagtatca caacccagta tcagggcacc
     2221 tgtcggtgtg cagggaccat ctacctgtgg aactggaatg acaaagtcat catctctgac
     2281 atcgacggaa caataaccaa gtctgatgct ttggggcaga ttctcccaca gctgggtaaa
     2341 gactggacgc atcagggcat agctaggctc taccattcca tcaatgagaa tggctacaag
     2401 tttctgtact gttctgcacg tgccatcggc atggccgaca tgacccgtgg ttatctgcac
     2461 tgggtcaatg ataaggggac gatcttgcct cgaggccctc tgatgctgtc tcccagcagc
     2521 ttgttctctg ccttccacag ggaagtgata gaaaagaaac cagagaagtt caaaattgag
     2581 tgtctgaatg atattaagaa cttgtttgcc ccgtccaggc agcccttcta tgctgccttt
     2641 ggaaaccgtc ccaacgatgt ctatgcttac acacaagtcg gagttccaga ctgtaggata
     2701 tttactgtga atccaaaggg tgaattaatc caagagagga ccaaagggaa caaatcatcg
     2761 tatcacaggc tgagtgagct tgtggaacac gtgttcccac ttctcagtaa ggagcagaat
     2821 tctgcctttc catgcccaga gttcagctcc ttctgctact ggcgagaccc aatccctgac
     2881 ctggacctgg atgacctggc ttgaacaagg cctcgatggc aagatggggg gcactggcac
     2941 tggccctctt acagaggaag acccaggagc tgacagtcag gaacctgctt tccagaacag
     3001 ggacggggac ccgaagctgg cactgccacc ttctttcagc ttcctaggcc accactcagc
     3061 acttagagta ataaggggcc ctggacctgc gttagaagcc cgtgctctaa agcctgcact
     3121 cctttacttg tgggctctca cgtgcttctg tggagttgtg tactgggcac cacgcagctg
     3181 cacatctaaa aaggaagaaa ccagaaactg aaaaagaata gaccagcaca ttgctacaca
     3241 aaagtaaaga gttcaaatct ccactggtca gttgggatcg tttctctctc taaccagggc
     3301 tttctccaga ggtccagtta gtgctgccag ctctgcgtgc acaccctcct cgggatgcct
     3361 ccactctcac tcactgaact aggacagctg cttcatgtgc cagcaccgtg agcccatctg
     3421 ccccagcatg ggctgtaaag ttggggggaa agcaccctag cagcagcgtc tcctcacctg
     3481 tccccgccgc agtgagaaag aagcgcttta agctgctgtt atgacctagc agaagaacaa
     3541 atgcacagtt catgtctgga gtgatggtga gaccttggtt tgaatcacaa agaaggcagt
     3601 ggcttctcag aagtgtagaa gggcagttgc ctggggtcct gggagtcctg ttctctgaca
     3661 ctgtcacctg acttaagtgg cccttggggc agattatttc agaacaggat ttttgaaagt
     3721 ctgggcttca tatttgctga ggagaaagcc tttggctagt ggcattgacc agtcatctgg
     3781 attccaacca tgtaatgcag tcatggaggc ctgggagaga gaggctcctg agtgcctgtt
     3841 gagagccaca cctcagcagc agcagcagcc catcactctg actcaccctc atgcagttga
     3901 tagtgcagat gaggcctcgt ggggatgtgg ggtacccccg cacctccctg gcggcctcag
     3961 acactggcct cgttaagtgc tcttgtcaaa tgttggagca gatggcacca gggagagtta
     4021 tgtcctcaga tctgcagagc cttgcccagc tgggtgtggg cagttactaa gaaatccatc
     4081 tgagttcgag gtcagcctgg tctacagagt tagttccagg acagccagga ctacacagag
     4141 aaaccctgtc ttgaagaaaa aagaaaaaaa gaaaaggggt ggctattttc cagattggag
     4201 agttgagtgt ccttgcctca tttcagtatt gcagttgaat gcagagacct tggtaacaat
     4261 gtttaattaa agaaaaagac catttgaaac atagttgtcc gtggcaagcc ctggaggcac
     4321 gtagtcccag agaagcaacg caggctcccg gcactgtgct gatgtttccc acaagtgttc
     4381 cctctgctcc gtgagttact tgtgttaggt agtagggaaa cttggctatg ttcatgtgac
     4441 gttctcgcca gttaagtgaa ataagactct actaaagcag ccacgggaga tttgaagcct
     4501 aagccatcac caccacagta agaattgtgc acttaacctg agcttggtag aatagccacg
     4561 gtacagaaaa tcctatgttt tctcttagac cttctgtttt tcctttaggg agtctttgcc
     4621 tcactagtct gtttcctttt cctaacagac attttctttg gacacacggg ccttttcaga
     4681 aggtccagcg taggctctgg gtctcctggc ctgtgcagag tgatggctgg tctttctgtc
     4741 cacgtgtctg ttagcgcgac actcgtccct gcacattgat gagtgggtat ttaggataat
     4801 gaatgtaact cttatttaaa ggagtctcct tgctgagagt agatgagtac tattactgta
     4861 gtgtatgaag tattaggttt gtgctgaaaa tccattgcca tttgttacaa atgacatttg
     4921 ttctttctgt gaaagagaga tgcccttaag tgtgtttgca cacaaacccc tggatggctt
     4981 gttgcagtgt caccttgtct cgatgacagg tgatgctgaa gagacactat ggcagaccag
     5041 ccacccagtc cttccgtcag ttgttgcgtc agttcagtct aattttagtg tttggtgttg
     5101 agtgttgaag cttgcactgc tgtagaattg atggctgctc tccgtcaggc tgtgtggggt
     5161 gtacacgtgg acctggagca tgcatgtgta accatatgat cagtactgca gttcctgtag
     5221 tctaacccat ctgcatagtc agttggaaaa agaaatgtgc tgttgtagta acgtagaaca
     5281 aaagctgcta aaacaatgta gagtggacca ggacccgagc tgagcagcag gagcctctgt
     5341 tccctgcagc ctgtggggcg ggtccagcct tctgaggtgc ccttcaaagc catcatctga
     5401 gagaagaaac ggttcaggcg acatgagcaa gtttctggga aactttgttt gagagcatgc
     5461 taaggtttat ttaaaacccc aggaattgct ctttccaggt gaaaaattct ttttttgacc
     5521 tgaaggcaga ggaaagagtt ccctgctaat gattccattc agctgatgaa gtacttgatt
     5581 attagagagc agtgccacta agcagcttta agttgtccgt aatcattgta aacatcagtg
     5641 cagtaccttc aatatggtaa agatgttgtc ttcagatgaa tggctcatat tttggtgcag
     5701 tgtttgatgt tcacaaaaaa atgttacaat aataaactaa cataaactga taaaaaaaaa
     5761 aaaaaaaa
//
