LOCUS       NM_029671                741 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus LLLL and CFNLAS motif containing 1 (Llcfc1),
            transcript variant 1, mRNA.
ACCESSION   NM_029671 XM_110591 XM_489711
VERSION     NM_029671.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Nishio,S., Emori,C., Wiseman,B., Fahrenkamp,D., Dioguardi,E.,
            Zamora-Caballero,S., Bokhove,M., Han,L., Stsiapanava,A.,
            Algarra,B., Lu,Y., Kodani,M., Bainbridge,R.E., Komondor,K.M.,
            Carlson,A.E., Landreh,M., de Sanctis,D., Yasumasu,S., Ikawa,M. and
            Jovine,L.
  TITLE     ZP2 cleavage blocks polyspermy by modulating the architecture of
            the egg coat
  JOURNAL   Cell 187 (6), 1440-1459 (2024)
   PUBMED   38490181
REFERENCE   2  (bases 1 to 741)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 741)
  AUTHORS   Matsumura,T., Noda,T., Satouh,Y., Morohoshi,A., Yuri,S., Ogawa,M.,
            Lu,Y., Isotani,A. and Ikawa,M.
  TITLE     Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma
            in Mice and Rats
  JOURNAL   Front Cell Dev Biol 9, 810118 (2022)
   PUBMED   35096839
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 741)
  AUTHORS   Noda,T., Lu,Y., Fujihara,Y., Oura,S., Koyano,T., Kobayashi,S.,
            Matzuk,M.M. and Ikawa,M.
  TITLE     Sperm proteins SOF1, TMEM95, and SPACA6 are required for
            sperm-oocyte fusion in mice
  JOURNAL   Proc Natl Acad Sci U S A 117 (21), 11493-11502 (2020)
   PUBMED   32393636
  REMARK    GeneRIF: Sperm proteins SOF1, TMEM95, and SPACA6 are required for
            sperm-oocyte fusion in mice.
REFERENCE   5  (bases 1 to 741)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 741)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156400.9.
            
            On Nov 17, 2006 this sequence version replaced NM_029671.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC048592.1, AK006606.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-238               AC156400.9         122410-122647       c
            239-741             AC156400.9         121361-121863       c
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 19.86 cM"
     gene            1..741
                     /gene="Llcfc1"
                     /gene_synonym="1700034O15Rik"
                     /note="LLLL and CFNLAS motif containing 1"
                     /db_xref="GeneID:76606"
                     /db_xref="MGI:MGI:1923856"
     exon            1..238
                     /gene="Llcfc1"
                     /gene_synonym="1700034O15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..414
                     /gene="Llcfc1"
                     /gene_synonym="1700034O15Rik"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; uncharacterized protein C7orf34 homolog;
                     MSSP-binding protein CTM-1; LLLL and CFNLAS
                     motif-containing protein 1; sperm-oocyte fusion required
                     protein 1; sperm-egg fusion protein LLCFC1"
                     /codon_start=1
                     /product="sperm-egg fusion protein LLCFC1 isoform 1
                     precursor"
                     /protein_id="NP_083947.1"
                     /db_xref="CCDS:CCDS20054.1"
                     /db_xref="GeneID:76606"
                     /db_xref="MGI:MGI:1923856"
                     /translation="MTSLGSQLHRATFLTALLLLLLLQVKGVKTLIVSASLDGDKSQK
                     DKVSSEDQGEEEYEEHFEASSEGEQWQEIDMVQQEDTISQAITLQDHLLDLAFCFNLA
                     SIMFFL"
     exon            239..741
                     /gene="Llcfc1"
                     /gene_synonym="1700034O15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      724..729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Llcfc1"
                     /gene_synonym="1700034O15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      730
                     /gene="Llcfc1"
                     /gene_synonym="1700034O15Rik"
                     /note="major polyA site"
ORIGIN      
        1 gctgtcaagg ggtaagagct aagaaaggga tggggtggtg ttggtagctg gggattgggg
       61 ccaaaggaag catgggagag gagacccatg acttctctgg gctcccagct ccacagagca
      121 acattcctga cagctttgtt gctactgctg ctgctccaag tgaagggggt gaagactctg
      181 atagtgagtg caagcttgga tggtgacaag agtcagaaag acaaagtgtc ctctgaagac
      241 cagggtgagg aggagtatga agaacacttt gaggcttcat cagagggtga gcagtggcag
      301 gagatagata tggtccagca agaagacacg atctcccagg caataactct ccaggaccac
      361 ctccttgacc tagccttctg cttcaacctg gccagcatca tgtttttttt atgagagact
      421 ttgaggtgga ggcagtagtc tggatccttc atgaggactt agacatccac ctccccttcc
      481 tgattgctta ctgacctcct ctcctaaatg cctcctcaaa atgcacaccc tggtccctgt
      541 ctctaagaag tacctgacct tagggtatat caacagagct ttcccaactc tcatatccac
      601 cacctaattg ccaccccaac ccagaatttc atgtcccggg gccaaagaca ccaaacccac
      661 acccttctct cttaagcttt gtctgaaaca gggaggagag agtgacaata aagtagcaat
      721 tgcaataaaa tcctgaaaac a
//
