LOCUS       NM_001377031            1604 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus lipoyltransferase 1 (Lipt1), transcript variant 4,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001377031 XM_006496176
VERSION     NM_001377031.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Solmonson,A., Faubert,B., Gu,W., Rao,A., Cowdin,M.A.,
            Menendez-Montes,I., Kelekar,S., Rogers,T.J., Pan,C., Guevara,G.,
            Tarangelo,A., Zacharias,L.G., Martin-Sandoval,M.S., Do,D.,
            Pachnis,P., Dumesnil,D., Mathews,T.P., Tasdogan,A., Pham,A.,
            Cai,L., Zhao,Z., Ni,M., Cleaver,O., Sadek,H.A., Morrison,S.J. and
            DeBerardinis,R.J.
  TITLE     Compartmentalized metabolism supports midgestation mammalian
            development
  JOURNAL   Nature 604 (7905), 349-353 (2022)
   PUBMED   35388219
REFERENCE   3  (bases 1 to 1604)
  AUTHORS   Ni,M., Solmonson,A., Pan,C., Yang,C., Li,D., Notzon,A., Cai,L.,
            Guevara,G., Zacharias,L.G., Faubert,B., Vu,H.S., Jiang,L., Ko,B.,
            Morales,N.M., Pei,J., Vale,G., Rakheja,D., Grishin,N.V.,
            McDonald,J.G., Gotway,G.K., McNutt,M.C., Pascual,J.M. and
            DeBerardinis,R.J.
  TITLE     Functional Assessment of Lipoyltransferase-1 Deficiency in Cells,
            Mice, and Humans
  JOURNAL   Cell Rep 27 (5), 1376-1386 (2019)
   PUBMED   31042466
REFERENCE   4  (bases 1 to 1604)
  AUTHORS   Cao,X., Zhu,L., Song,X., Hu,Z. and Cronan,J.E.
  TITLE     Protein moonlighting elucidates the essential human pathway
            catalyzing lipoic acid assembly on its cognate enzymes
  JOURNAL   Proc Natl Acad Sci U S A 115 (30), E7063-E7072 (2018)
   PUBMED   29987032
REFERENCE   5  (bases 1 to 1604)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC119189.8.
            
            [WARNING] On Jul 30, 2024 this sequence was replaced by
            NM_001377031.2.
            
            On Dec 9, 2019 this sequence version replaced XM_006496176.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.154817.1,
                                           SRR5189679.63318.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-239               AC119189.8         110537-110775       c
            240-1604            AC119189.8         106897-108261       c
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 16.13 cM"
     gene            1..1604
                     /gene="Lipt1"
                     /gene_synonym="EG623661"
                     /note="lipoyltransferase 1"
                     /db_xref="GeneID:623661"
                     /db_xref="MGI:MGI:3645211"
     exon            1..239
                     /gene="Lipt1"
                     /gene_synonym="EG623661"
                     /inference="alignment:Splign:2.1.0"
     exon            240..1604
                     /gene="Lipt1"
                     /gene_synonym="EG623661"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..1362
                     /gene="Lipt1"
                     /gene_synonym="EG623661"
                     /EC_number="2.3.1.200"
                     /note="lipoyltransferase 1, mitochondrial; lipoyl ligase;
                     lipoate-protein ligase; lipoate biosynthesis protein;
                     lipoyl amidotransferase LIPT1, mitochondrial"
                     /codon_start=1
                     /product="lipoyl amidotransferase LIPT1, mitochondrial
                     precursor"
                     /protein_id="NP_001363960.1"
                     /db_xref="GeneID:623661"
                     /db_xref="MGI:MGI:3645211"
                     /translation="MLIPLSMKNCFRLLCQHKVPAAGFKSPPTHGLILQSISNDVYEN
                     LAFEDWIHDHIHLEGKPILFLWRNSPSVVIGRHQNPWQECNLHLMRQEGIKLARRKSG
                     GGAVYHDMGNINLTFFTTKTKYDRMENLKLIVRALNAVQPQLDVQPTKKFDLLLDGQF
                     KISGTASKIGRTAAYHHCTLLCSTNRTALSSSLKSPYCGIKSNATPSIPSAVKNLLER
                     DSTLTCEVLMSAVAAEYAAHHQVDGHVNLINPADETMFPGINRKVKELQSWEWVYGRT
                     PKFTVDTTFHVPYEQAHLEIQVFMDVKNGRIETCAIKAPDHWLPLEIGEKLNSSFIGS
                     KFCPVETTLLTNVLLRTCPGDHHLHSKWYILCEKIRGIM"
     regulatory      1585..1590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lipt1"
                     /gene_synonym="EG623661"
                     /note="hexamer: ATTAAA"
     polyA_site      1604
                     /gene="Lipt1"
                     /gene_synonym="EG623661"
                     /note="major polyA site"
ORIGIN      
        1 agtgaattct ttgcgggttg tggaggcaca cgctgagcag gagtgggtgc tgcggagagc
       61 acgcgtgcgt cccatgggcc cctcctattg gttctgaaag ctctgcagat tacacctggt
      121 gctccggacg taatttcatt attgggaaca gttgatttct aatacggcgt agggtaaact
      181 aaacaagaca ttcagtgcta gtcgcgtgcc tgctcccgaa gagtaaagct tgcttaaaac
      241 atgctaattc cactttcaat gaagaattgc ttccgattac tttgtcagca caaggtccca
      301 gcagctggct ttaaaagccc acccacacat gggctcattt tacagtcgat ctccaatgat
      361 gtatatgaaa atctggcttt tgaagactgg atccacgacc acatacatct agaaggcaag
      421 ccgattcttt tcctttggag aaattctcct tctgtcgtca tcggcaggca tcagaatccg
      481 tggcaggaat gtaacctcca tctgatgaga caggaaggaa tcaaactggc tcggaggaag
      541 agtggaggag gagccgtgta ccacgacatg ggtaacatca acctcacctt ctttaccacc
      601 aaaaccaagt atgacagaat ggaaaatctg aaactaatcg tgagagctct gaatgctgtc
      661 caaccccagc tggatgtgca gcctaccaaa aagtttgacc tgctactcga cgggcagttt
      721 aaaatctcag gaaccgcatc aaagatcggc cggactgccg cgtaccacca ctgcaccttg
      781 ctatgcagca ccaaccggac agctctgtct tcttcgctga agagcccgta ctgcgggata
      841 aagagcaacg ccactcccag catcccttcc gctgtcaaaa accttctgga aagagattcc
      901 acgctgacct gtgaagttct gatgagtgcg gtggctgcag agtacgcggc gcatcatcaa
      961 gttgatggtc acgtcaacct aataaaccct gcagatgaga cgatgttccc tggaataaac
     1021 cgcaaagtca aggaactgca aagttgggag tgggtatacg gcaggactcc caagtttacc
     1081 gtggacacca cgtttcatgt gccgtatgag caggcgcatt tggaaatcca agtgttcatg
     1141 gatgtaaaga acggacgaat cgaaacctgc gctattaagg cacccgatca ctggctgccg
     1201 ctggaaatag gtgagaaatt aaactcaagt ttcatcggca gcaaattctg ccccgttgaa
     1261 accaccctgc taacgaatgt attactgaga acatgtccag gcgaccatca cttacacagt
     1321 aaatggtaca ttctgtgtga aaaaatcagg gggataatgt gaccctaaca actttgtcta
     1381 cactggcagt tttggtgaga aaaaaacttt aaactacaat ttgtcttata tcttaacaga
     1441 aataggggtg tcagtttgaa cctgtgttgg tcttgaggtg gcctgtccct aatttcaact
     1501 cctcagcttt tgtcagactt tgattgataa tctgttaagg gttttggcac tttcagggcc
     1561 tcgtcctctt ccatttctcc tctcattaaa cttcagagaa gaaa
//
