LOCUS       NM_010718               4537 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus LIM domain kinase 2 (Limk2), transcript variant 1,
            mRNA.
ACCESSION   NM_010718
VERSION     NM_010718.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Gong,C., Chang,L., Huang,R., Sun,X., Liu,Y., Wu,S., Wang,L., Xu,B.
            and Wang,L.
  TITLE     LIM kinase 2 activates cardiac fibroblasts and exacerbates
            postinfarction left ventricular remodeling via crosstalk between
            the canonical and non-canonical Wnt pathways
  JOURNAL   Pharmacol Res 208, 107347 (2024)
   PUBMED   39153710
  REMARK    GeneRIF: LIM kinase 2 activates cardiac fibroblasts and exacerbates
            postinfarction left ventricular remodeling via crosstalk between
            the canonical and non-canonical Wnt pathways.
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4537)
  AUTHORS   Malvi,P., Reddy,D.S., Kumar,R., Chava,S., Burela,S., Parajuli,K.,
            Zhang,X. and Wajapeyee,N.
  TITLE     LIMK2 promotes melanoma tumor growth and metastasis through
            G3BP1-ESM1 pathway-mediated apoptosis inhibition
  JOURNAL   Oncogene 42 (18), 1478-1491 (2023)
   PUBMED   36922679
  REMARK    GeneRIF: LIMK2 promotes melanoma tumor growth and metastasis
            through G3BP1-ESM1 pathway-mediated apoptosis inhibition.
REFERENCE   4  (bases 1 to 4537)
  AUTHORS   Zhou,Z., He,G., Zhang,X., Lv,X., Zhang,X., Liu,A., Xia,S., Xie,H.,
            Dang,R., Han,L., Qi,J., Meng,Y., Yu,S., Xie,W. and Jia,Z.
  TITLE     NGPF2 triggers synaptic scaling up through
            ALK-LIMK-cofilin-mediated mechanisms
  JOURNAL   Cell Rep 36 (7), 109515 (2021)
   PUBMED   34407403
  REMARK    GeneRIF: NGPF2 triggers synaptic scaling up through
            ALK-LIMK-cofilin-mediated mechanisms.
REFERENCE   5  (bases 1 to 4537)
  AUTHORS   Dennison,B.J.C., Larson,E.D., Fu,R., Mo,J. and Fantauzzo,K.A.
  TITLE     Srsf3 mediates alternative RNA splicing downstream of PDGFRalpha
            signaling in the facial mesenchyme
  JOURNAL   Development 148 (14) (2021)
   PUBMED   34184034
REFERENCE   6  (bases 1 to 4537)
  AUTHORS   Takahashi,H., Koshimizu,U. and Nakamura,T.
  TITLE     A novel transcript encoding truncated LIM kinase 2 is specifically
            expressed in male germ cells undergoing meiosis
  JOURNAL   Biochem Biophys Res Commun 249 (1), 138-145 (1998)
   PUBMED   9705845
REFERENCE   7  (bases 1 to 4537)
  AUTHORS   Ikebe,C., Ohashi,K. and Mizuno,K.
  TITLE     Identification of testis-specific (Limk2t) and brain-specific
            (Limk2c) isoforms of mouse LIM-kinase 2 gene transcripts
  JOURNAL   Biochem Biophys Res Commun 246 (2), 307-312 (1998)
   PUBMED   9610354
REFERENCE   8  (bases 1 to 4537)
  AUTHORS   Zambrowicz,B.P., Friedrich,G.A., Buxton,E.C., Lilleberg,S.L.,
            Person,C. and Sands,A.T.
  TITLE     Disruption and sequence identification of 2,000 genes in mouse
            embryonic stem cells
  JOURNAL   Nature 392 (6676), 608-611 (1998)
   PUBMED   9560157
REFERENCE   9  (bases 1 to 4537)
  AUTHORS   Koshimizu,U., Takahashi,H., Yoshida,M.C. and Nakamura,T.
  TITLE     cDNA cloning, genomic organization, and chromosomal localization of
            the mouse LIM motif-containing kinase gene, Limk2
  JOURNAL   Biochem Biophys Res Commun 241 (2), 243-250 (1997)
   PUBMED   9425257
REFERENCE   10 (bases 1 to 4537)
  AUTHORS   Ikebe,C., Ohashi,K., Fujimori,T., Bernard,O., Noda,T.,
            Robertson,E.J. and Mizuno,K.
  TITLE     Mouse LIM-kinase 2 gene: cDNA cloning, genomic organization, and
            tissue-specific expression of two alternatively initiated
            transcripts
  JOURNAL   Genomics 46 (3), 504-508 (1997)
   PUBMED   9441759
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY253265.1, AK146099.1 and AK034900.1.
            
            On Oct 18, 2013 this sequence version replaced NM_010718.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB008117.1, AB005131.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-373               BY253265.1         1-373
            374-1378            AK146099.1         463-1467
            1379-4537           AK034900.1         41-3199
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 2.34 cM"
     gene            1..4537
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /note="LIM domain kinase 2"
                     /db_xref="GeneID:16886"
                     /db_xref="MGI:MGI:1197517"
     exon            1..193
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..2094
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /EC_number="2.7.11.1"
                     /note="isoform a is encoded by transcript variant 1; LIM
                     kinase 2; LIM motif-containing protein kinase 2"
                     /codon_start=1
                     /product="LIM domain kinase 2 isoform a"
                     /protein_id="NP_034848.1"
                     /db_xref="CCDS:CCDS24358.1"
                     /db_xref="GeneID:16886"
                     /db_xref="MGI:MGI:1197517"
                     /translation="MAALAGDEAWRCRGCGTYVPLSQRLYRTANEAWHGSCFRCSECQ
                     ESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCK
                     VIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTE
                     CRRGFSVTVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVE
                     EVEDAIKQTSQTLQLLIEHDPVPQRLDQLRLDARLPPHMQSTGHTLMLSTLDTKENQE
                     GTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHG
                     EVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFI
                     GVLYKDKKLNLLTEYIEGGTLKDFLRSVDPFPWQQKVRFAKGISSGMAYLHSMCIIHR
                     DLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKATTKKRTLRKSDRKKRYTVVG
                     NPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEK
                     FVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFLGELAIPLPAELEDLDH
                     TVSMEYGLTRDSPP"
     exon            194..293
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            294..429
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            430..539
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            540..728
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            729..834
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            835..1031
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1218
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1305
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1437
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1494
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1560
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1735
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1791
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1949
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..4537
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2337..2342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /note="hexamer: GATAAA"
     polyA_site      2357
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
     regulatory      3359..3364
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /note="hexamer: AATGAA"
     polyA_site      3378
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /note="major polyA site"
     regulatory      3555..3560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /note="hexamer: AATAAA"
     polyA_site      3578
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /note="major polyA site"
     regulatory      4438..4443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /note="hexamer: AATACA"
     polyA_site      4455
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
     regulatory      4514..4519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
                     /note="hexamer: AATAAA"
     polyA_site      4531
                     /gene="Limk2"
                     /gene_synonym="Limk-2; whe"
ORIGIN      
        1 gaggggctcc ggggcgggtc cccgtgccag gtgcggtttg gtcctcccag ggcgccgtag
       61 gcggtgcatc cttccggctg caggggcaga gacagcctct tctctagacc gcaggcgggc
      121 gcggacctga ggcgctgctg ccgggtggtc cttcctgtgt tgtccgcgcc ctggaccatg
      181 gcggcgctgg cgggtgatga agcctggagg tgtcggggct gtggcaccta tgttcccctg
      241 agccagaggc tctataggac tgccaatgaa gcctggcatg gctcctgctt ccggtgttcc
      301 gaatgccagg aatccctcac caactggtac tatgagaagg atgggaagct gtactgccac
      361 aaggactact gggccaagtt tggagaattc tgccatgggt gctccctgct gatgacgggg
      421 ccagccatgg tggctgggga gtttaagtac cacccagagt gctttgcctg catgagttgc
      481 aaggtgatca ttgaggatgg agatgcatat gccctggtgc agcacgctac actctactgt
      541 gggaagtgcc acaacgaggt ggtgctggca cccatgtttg aaagactgtc tacagagtct
      601 gtccaggatc agctgcccta ctctgtcacg ctcatctcca tgcctgctac caccgagtgt
      661 agacgcggct tctcggtgac tgtggaaagt gcctcttcta actatgccac caccgttcaa
      721 gtgaaagagg tcaaccggat gcacatcagt cccaacaacc gaaatgccat ccaccctggg
      781 gaccgcatct tggagatcaa tgggacccct gtccgtactc tgcgagtaga ggaggtggag
      841 gatgcaataa agcagacaag ccagacactt cagctgctga ttgaacatga ccctgtgccc
      901 cagcgcctgg accagctacg gctagatgcc cgacttcctc cccacatgca gagcaccgga
      961 cacacgctca tgctcagcac cttggacacc aaggagaatc aggaggggac actgaggaga
     1021 cgttctctga ggcgcagtaa cagcatctcc aagtctccgg gccccagctc ccccaaggag
     1081 cccctgctcc tcagccggga cataagccgc tcagaatccc tccgctgctc tagcagctac
     1141 tcccagcaga tcttccggcc ctgcgacctg atccacgggg aggtcctggg gaagggcttc
     1201 tttgggcagg ccatcaaggt gactcacaaa gccacaggca aagtgatggt catgaaggag
     1261 ttgatccgct gtgatgagga aacccagaag actttcctga ctgaggtgaa agtgatgcgg
     1321 agcctggacc accctaatgt gctcaagttc atcggagttc tgtacaagga caagaagctg
     1381 aatctgctga ccgagtacat cgagggaggc acactcaaag acttcctgcg cagtgtggac
     1441 ccgttcccct ggcaacagaa ggtcaggttt gccaaaggca tctcctctgg aatggcctat
     1501 ttacactcca tgtgcatcat ccaccgggac ctgaactcac acaactgtct catcaaattg
     1561 gacaagacag tggtagtagc cgactttggg ctgtcacggc ttatagtaga agagaggaaa
     1621 aggcctccag tagagaaggc caccaccaag aaacgcacct tacgcaagag tgaccgcaaa
     1681 aagcgctaca ctgtggtggg aaacccctac tggatggccc ccgagatgct gaatggcaag
     1741 agctacgatg agacggtgga tgtcttctct tttgggatcg ttctctgtga gatcattggg
     1801 caggtatatg cggatcctga ttgcctgccc cgcacactgg actttggcct caatgttaag
     1861 cttttctggg agaagtttgt cccaacagac tgtcccccag ccttcttccc cctggctgct
     1921 atctgctgca aactagagcc tgaaagcaga ccggcattct caaagctgga ggactcgttc
     1981 gaggcgctct ccctgttctt gggggagctg gccatcccac tgccagcaga gctggaagac
     2041 ctggaccaca ctgtgagcat ggagtatggc ctaacccggg actcgccacc ctagccctgg
     2101 tccctccccc tgcaggggga cattccacag ccagcattgc cccctctgca cgctgtcctt
     2161 gctgtgagca ggactgccta ggtttcctgt ggattgaagg cttgcttaga ggcacaacaa
     2221 gccatcccta ctacctcccc aggaggcact tgggtgcagg gaaaaaaatt ctccgcaagt
     2281 tttgggggcc tagtttctat ctgtaaatcc aacactcacc tgaaagctgt gaagaggata
     2341 aaagagcctg gattatagac caagagtctg ttactcggga ctacttggga cctctgtgga
     2401 ttgagattct gggaggctgt agctttacac taatcaacat gatctgggcc tgctgggcaa
     2461 gatgacggag tgaactggcc tgagggctct ggagtctggc tcagcaaggt gcaggacttg
     2521 aagcggtagt atgagttaca gaaacgggga accttcaaag catgtgaaat gacagtagcg
     2581 atttctcctc catcccaggc catgcccttt ggagcaggtt ttaaagagtc ttagtatgtg
     2641 gtaaaaccta cagaatccaa caaaggactg gtctctgctc acagcccagg gaccacatca
     2701 gtgcatgggg agtttccact tcagcatcct gtctcaacaa gcagtcactc acaaaaacac
     2761 caaagaaact ggggctgcaa acaggccctg ccctctaaaa gccctgtatc caggctggtg
     2821 ggctcagtca aaagctcagc atgttgccta acagtacatg ggttctggat gccagcctgg
     2881 tttgcctcag actttgggtc taagcagaga gaagggggtc ccatcaattt ctttgcaccc
     2941 ttgtggcagc tactgcacat cctctagaat gtctgggcca ggcagcaatg tgggacaggc
     3001 attggtggct gctgagtccc caaactgaga taggaaaaga acaccctgag ctgggccttg
     3061 tagattctac ctctttttgt tgctggtcca gcttgcttcc tgagccacac tcgagggcca
     3121 tgggcatggg caactatggg ggcactttct acccctggct gccaggtgtc cagggactct
     3181 gtagctcacc aaggtttgtc atctaagcgg gagctcacgc catgtttaac aaattgggaa
     3241 tgggtttggt tataaataca tgtgatgggt tgtactggtg gggggattca ggaagagtgg
     3301 ttgctgttat tagcttttat ctctggggtg atatgtgaga tattttgtac atagacgaaa
     3361 tgaattatgg gatgggaagt ctttggccac ttttcttgct gtgtagactg tacttgcgtg
     3421 tggagtttgc acacttgctg cagagggaat cctgaactcc cgatgggcat ggttcagcgt
     3481 ccttgactga gtatgcttgc actgtcccca gcaagtgtgg gagtggggcc tgcactaggc
     3541 catgaagcag actaaataaa ttaagcagtt aacatagaga tgacagagag tgccaaagtg
     3601 acttagggcc tgacttaaac acggaggaca ggatggataa tcacggtctg accagggttt
     3661 ggttcccagc acccacgtgg tggctctcta acatctgtac gtccagttct aggggtactc
     3721 tacctcatct cacctctgtg ggctcctgca catacatggt acacagacat gctctgggta
     3781 cataaattcc cattttaaag tgttttaaag aaacatggtc tgctggccac tttcatataa
     3841 cttaccagga aattctgtct caagtttaat cccctcacct ggacaacaac ccttcagaga
     3901 tcatatgcat ccaccggagc ccctctgagg agcagccgat gacctcatct tagacccgca
     3961 aagctatcag gttctcagct gcttacccag tcctcagggg ccttctgctt ccaaagctgt
     4021 tgcaccgacc actggcccaa taatgccaat gtttggtaag atcctgggca ccaaagaaag
     4081 agaaaaagaa gccccacagc ctctgccaag tgtggaagtg tgtgtttata agtctacacg
     4141 caagatacag aggctgggga accaagttca aggccagcct aagtctgtcg agtgaatttt
     4201 gggccagcta tggctaaagc actgaaagcc aggtctaata gctttgtggc cctaaaatga
     4261 agtcattttg tatataaacg taaagataat gtctcctaag cgtcctgtgg tagaagactt
     4321 tagattaaga gaatgtatgt ttggcagtgt tggtggcaca cacctttagt cccagcagag
     4381 gcaggtgtat ctgaggccag cctgagccat tacacagaga aaccaagaat ggaataaaat
     4441 acagtcccta gtaaacacct catctgaggt tctcagtgct gggctacaac tcaatctgct
     4501 gctgaggtca actaataaaa cttagacacc agtcctg
//
