LOCUS       NM_023063               4108 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus LIM domain and actin binding 1 (Lima1), transcript
            variant b, mRNA.
ACCESSION   NM_023063
VERSION     NM_023063.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Li,S., Yang,F., Cheng,F., Zhu,L. and Yan,Y.
  TITLE     Lipotoxic hepatocyte derived LIMA1 enriched small extracellular
            vesicles promote hepatic stellate cells activation via inhibiting
            mitophagy
  JOURNAL   Cell Mol Biol Lett 29 (1), 82 (2024)
   PUBMED   38822260
  REMARK    GeneRIF: Lipotoxic hepatocyte derived LIMA1 enriched small
            extracellular vesicles promote hepatic stellate cells activation
            via inhibiting mitophagy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4108)
  AUTHORS   Duethorn,B., Groll,F., Rieger,B., Drexler,H.C.A., Brinkmann,H.,
            Kremer,L., Stehling,M., Borowski,M.T., Mildner,K., Zeuschner,D.,
            Zernicka-Goetz,M., Stemmler,M.P., Busch,K.B., Vaquerizas,J.M. and
            Bedzhov,I.
  TITLE     Lima1 mediates the pluripotency control of membrane dynamics and
            cellular metabolism
  JOURNAL   Nat Commun 13 (1), 610 (2022)
   PUBMED   35105859
  REMARK    GeneRIF: Lima1 mediates the pluripotency control of membrane
            dynamics and cellular metabolism.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4108)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4108)
  AUTHORS   Zhang,Y.Y., Fu,Z.Y., Wei,J., Qi,W., Baituola,G., Luo,J., Meng,Y.J.,
            Guo,S.Y., Yin,H., Jiang,S.Y., Li,Y.F., Miao,H.H., Liu,Y., Wang,Y.,
            Li,B.L., Ma,Y.T. and Song,B.L.
  TITLE     A LIMA1 variant promotes low plasma LDL cholesterol and decreases
            intestinal cholesterol absorption
  JOURNAL   Science 360 (6393), 1087-1092 (2018)
   PUBMED   29880681
  REMARK    GeneRIF: this study identifies LIMA1 as a key protein regulating
            intestinal cholesterol absorption.
REFERENCE   6  (bases 1 to 4108)
  AUTHORS   Leitner,L., Shaposhnikov,D., Descot,A., Hoffmann,R. and Posern,G.
  TITLE     Epithelial Protein Lost in Neoplasm alpha (Eplin-alpha) is
            transcriptionally regulated by G-actin and MAL/MRTF coactivators
  JOURNAL   Mol Cancer 9, 60 (2010)
   PUBMED   20236507
  REMARK    GeneRIF: Eplin-alpha transcription is regulated by actin-MAL-SRF
            signalling.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4108)
  AUTHORS   Abe,K. and Takeichi,M.
  TITLE     EPLIN mediates linkage of the cadherin catenin complex to F-actin
            and stabilizes the circumferential actin belt
  JOURNAL   Proc Natl Acad Sci U S A 105 (1), 13-19 (2008)
   PUBMED   18093941
  REMARK    GeneRIF: EPLIN functions to link the cadherin-catenin complex to
            F-actin and simultaneously stabilizes this population of actin
            fibers, resulting in the establishment of the adhesion
REFERENCE   8  (bases 1 to 4108)
  AUTHORS   Han,M.Y., Kosako,H., Watanabe,T. and Hattori,S.
  TITLE     Extracellular signal-regulated kinase/mitogen-activated protein
            kinase regulates actin organization and cell motility by
            phosphorylating the actin cross-linking protein EPLIN
  JOURNAL   Mol Cell Biol 27 (23), 8190-8204 (2007)
   PUBMED   17875928
  REMARK    GeneRIF: ERK-mediated phosphorylation of EPLIN contributes to actin
            filament reorganization and enhanced cell motility.
REFERENCE   9  (bases 1 to 4108)
  AUTHORS   Song,Y., Maul,R.S., Gerbin,C.S. and Chang,D.D.
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3
            cells by epithelial protein lost in neoplasm (EPLIN) requires
            localization of EPLIN to actin cytoskeleton
  JOURNAL   Mol Biol Cell 13 (4), 1408-1416 (2002)
   PUBMED   11950948
  REMARK    GeneRIF: EPLIN may function in growth control by associating with
            and regulating the actin cytoskeleton
REFERENCE   10 (bases 1 to 4108)
  AUTHORS   Maul,R.S., Sachi Gerbin,C. and Chang,D.D.
  TITLE     Characterization of mouse epithelial protein lost in neoplasm
            (EPLIN) and comparison of mammalian and zebrafish EPLIN
  JOURNAL   Gene 262 (1-2), 155-160 (2001)
   PUBMED   11179679
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134548.5.
            
            On Sep 13, 2022 this sequence version replaced NM_023063.4.
            
            Transcript Variant: This variant (b) uses an alternate 5'-terminal
            exon, lacks part of the 5' coding region, and initiates translation
            from a downstream start codon, compared to variant a. The encoded
            isoform (b) has a shorter N-terminus, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK085065.1, AK132314.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-649               AC134548.5         151766-152414       c
            650-734             AC134548.5         139931-140015       c
            735-883             AC134548.5         138695-138843       c
            884-985             AC134548.5         134383-134484       c
            986-1043            AC134548.5         129014-129071       c
            1044-1153           AC134548.5         126681-126790       c
            1154-1287           AC134548.5         115792-115925       c
            1288-4108           AC134548.5         110742-113562       c
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 56.13 cM"
     gene            1..4108
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /note="LIM domain and actin binding 1"
                     /db_xref="GeneID:65970"
                     /db_xref="MGI:MGI:1920992"
     exon            1..649
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     CDS             500..2281
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /note="isoform b is encoded by transcript variant b; LIM
                     domain and actin-binding protein 1; epithelial protein
                     lost in neoplasm"
                     /codon_start=1
                     /product="LIM domain and actin-binding protein 1 isoform
                     b"
                     /protein_id="NP_075550.2"
                     /db_xref="CCDS:CCDS37206.1"
                     /db_xref="GeneID:65970"
                     /db_xref="MGI:MGI:1920992"
                     /translation="MENCLGDSRHEAEKPETSENTETSGKIEKYNVPLNRLKMMFEKG
                     EHNQTKSLWTQSRNAGGRRLSENNCSLDDWEIGAGHLSSSAFNSEKNESKRNLELPRL
                     SETSIKDRMAKYQAAVSKQSSPASYTNELKTSESKTHKWEQKENVPPGPEACSVHQEG
                     SKVSTTENSLVALSVPAEDDTCNSQVKSEAQQPMHPKPLSPDARTSSLPESSPSKTAK
                     KFQAPAKESCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYC
                     KPHFNQLFKSKGNYDEGFGHKQHKDLWASKSDNEETLGRPAQPPNAGESPHSPGVEDA
                     PIAKVGVLAASMEAKASSQREREDKPAETKKLRIAWPPPAELGGSGSALEEGIKVSKP
                     KWPPEDDVCKTEAPEDVDLDLKKLRRSSSLKERSRPFTVAASFRTSSIKSPKASSPSL
                     RKGWSESEQSEEFGGGIATMERKQTENARPSGEKENVGKSRWQGEEVPRSKDRSSFEL
                     ESENFMENGANIAEDDNHVHAQQSPLEPEAPGWSGFVDTTAAKEFTTQNQKSQDVGFW
                     EGEVVRELSVEEQIKRNRYYDEDEDEE"
     exon            650..734
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            735..883
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            884..985
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1043
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1153
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1287
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..4108
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4089..4094
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /note="hexamer: AATAAA"
     polyA_site      4108
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /note="major polyA site"
ORIGIN      
        1 ctcttcctct ctctgggccc tgggtggatg ctcgctcctt gtggcagggc agtaactgtt
       61 cacagctgtg tgaacgacgg tggaccaggc acagcttgga gttttaactt tcagtttaca
      121 aagacgcatg tttggctgtt gcagcaggca agttgtaact ggtacttact tcttgttcct
      181 ctagaaccct gaaagtctgc cccagcactt tagaagaggg accctgtctg tgttaaagaa
      241 gaagtgggag aacccggtgg ctggggcaga attccacaca gactcactgc caaacagcag
      301 cagtgagggt gggcacacag cggactaccc tcctgctgaa gtgacggaca agcctgctcc
      361 tggagtcaga gctgaccggg aagagcacac ccagcccaaa cctagatttg gatctcgtcc
      421 ggaagcagtt atccagagcc ggtatcctcg ctcagagaac agccacgatt ttaaagccca
      481 ggccacggag agccaaaaaa tggaaaactg tctgggagat tccaggcatg aagcagagaa
      541 gccagagacg agcgaaaaca cagaaacttc aggcaaaata gagaaataca acgttccact
      601 gaatagactg aagatgatgt ttgagaaagg tgaacacaac caaaccaaga gtctctggac
      661 ccaaagccga aatgcgggtg gaaggaggct ctctgaaaac aactgttccc tggatgactg
      721 ggaaataggt gcgggtcatt tgtcttcctc tgcattcaac tcggagaaaa acgagagtaa
      781 gaggaatctg gagctgccac gcctctcaga aacctccata aaggaccgca tggccaagta
      841 ccaggctgca gtgtccaagc agagcagccc agccagctac acaaatgagc tgaaaaccag
      901 tgaaagcaaa actcataaat gggaacagaa ggagaatgtg cccccaggtc ccgaggcctg
      961 cagcgtccat caggaaggaa gcaaggtttc tacaactgag aatagtctag tggccctctc
     1021 tgtccctgct gaagatgaca cctgtaactc ccaggtgaag agcgaggccc agcagcctat
     1081 gcaccctaag ccgctgagtc ctgacgccag gacctccagt cttccggaaa gttctccttc
     1141 caaaacagcg aagaagtttc aggcgccggc aaaggagagc tgcgtggagt gtcagaagac
     1201 ggtgtacccc atggaacggc tcctggccaa ccagcaggtg tttcacatca gctgtttccg
     1261 atgctcctac tgcaacaaca agctcagtct aggaacatat gcatccttac atggacgaat
     1321 ctactgtaag cctcacttca atcaactctt caagtccaaa ggcaactatg acgagggctt
     1381 tgggcacaag cagcataagg atctgtgggc aagcaagagc gacaatgagg agactttggg
     1441 gagaccagcc cagccgccta atgcagggga gagcccccat agcccgggtg tagaagatgc
     1501 ccccatcgcc aaggtcggcg tgctggcggc aagtatggaa gccaaggcct cttctcagag
     1561 ggagagggag gataagcctg cggagaccaa gaagctgagg atcgcctggc ctccgccagc
     1621 cgagctgggc ggttccggaa gtgccctgga ggaagggatc aaagtatcga agcccaagtg
     1681 gcctccggag gatgacgtct gcaagacgga ggccccggag gatgtagatc tggacctgaa
     1741 gaagctgcgg cggtcttcgt ccctgaagga gaggagccgc ccgtttacgg tagcagcttc
     1801 ctttcgaacc tcttccatca agagccccaa ggcctcttcg ccatctctca ggaaaggttg
     1861 gagcgagtcc gagcagagtg aggagtttgg aggagggata gcgacgatgg aaaggaaaca
     1921 aacggaaaat gccagaccct ctggggagaa agagaatgtg ggaaaatcac gctggcaggg
     1981 cgaggaagtg ccgaggagca aggaccgtag ttcttttgag ctggagagtg agaattttat
     2041 ggaaaatgga gcaaacatag ctgaagatga caaccatgtc cacgcacagc agtccccgtt
     2101 agaacccgag gctccgggtt ggtctggctt tgtagacacc acggctgcta aagaattcac
     2161 tacccagaat caaaaatccc aagatgtcgg gttctgggag ggagaagtgg tccgagagct
     2221 gtctgtggag gaacagataa agagaaaccg gtactatgat gaggacgagg atgaagaatg
     2281 accggccgcc atggttctgg ccttagattt tggttagcaa ctcactgccc tttatcaagt
     2341 ggtgcgcaga aacaggcagc ttggcgccaa gggtcattca tgtgggagca actttggaaa
     2401 tgaattcccg ctagcaaaac agggcaaaaa caagcaaaaa caaaacagac aaacaaactc
     2461 acccaaaacc aattctaagc gcattgtcaa aacaccagtg atcttttaag cctggtgtgg
     2521 tggtaaacac ctttaatccc agcactccac tcgggaggca gaggcagagg caggtgggtc
     2581 tctctgagtt ccaggacagc cagggctaca aagagaaacc ttgcctcaaa acaaacaaac
     2641 aaacaaatcc ctccattttt tcagggaagg gctaagtagg gatttctctc actgatgctg
     2701 cctgccatcc actgcattcc ccaaatcaag attggtgtgg ttagatattt agaggcttgt
     2761 tttcacacta cagagaccat tgtggaatta gagacacaca caacacattt aggagcccac
     2821 ctcatgacca gaggcacttt agtataaagg gcacagtgta gctattttac caatttcatt
     2881 ttttagtact caattgctga aaatcaaata tttagtcttt caagcttttg aaatgcattc
     2941 tcttcctctg gtagataggg aaagcttact gtctttcatc ctacatcctg tatttttaat
     3001 cactgaggca caaaacagca acctttggcc aagtgaaagg cgctggttac tgaagccgga
     3061 gcagcagtgt tcaatcccca aaacctacgt aaaggtgaga gagcagactg cacagagctg
     3121 tccacacgtg cccatcatca acaataagaa acaagttaaa aaaaaaaata caaccaacct
     3181 cacaaacacc tgcgagacag agaaccaacc tatatacttt ggtgtttgga gagacggaca
     3241 ttgtacccag ataatcagtg gcgaatgcca gtgtgtctcc ctgcactctg ggtgtttgcc
     3301 atctcttctg gtggtttctc gcactttgcg gaaagctgca gtgttgctgt aagacctttt
     3361 ggatcacgct tcaccatgct ccaagtaaaa caacctgaga tcagagctgg gaagcctcag
     3421 gtcgaggcga atcttggctc tgaaatgtat tgatttttag gccagataat tcttccaggg
     3481 ttgcattaaa tcacttttag gtctgtcttc ttaggccgtg tcctactttg attcattgta
     3541 gtgataaaac actgggcagg agcaactcag aggggaaggg tttgtctcca ctcacaggcc
     3601 acgggtcaca cacaggccac gcgcgcacgc agcatggtca agggaagcga ggccagaacc
     3661 cagaggcaag agctgatgca gaccgtgtgg gaacactgct tgctgacttc atctctgtgg
     3721 cttactcaac tggttttctt acaccaccca gggccacctg gccaggggtg acactgctca
     3781 caacaaaaac agacaaggcc cacaggtgca tcggttagag gcggttcatc agctgagtcc
     3841 cacatccagg tgactctgtg tgaggctggt aaacccagag gaggatagat ggttttctct
     3901 ggtattaagg ctttagaata ttataaacga gtgcagttct tgtatgtaac tgatattcca
     3961 aatgatgtgt atggcggggt gggcttaagg tagtggcctc tttgcatttg tagagattta
     4021 gctcagtttt agagatgtaa aataatctgt ttccttatgg ccttgtttag cctaaaacat
     4081 ttttattcaa taaagctttt aatttaca
//
