LOCUS       NM_001113545            4198 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus LIM domain and actin binding 1 (Lima1), transcript
            variant a, mRNA.
ACCESSION   NM_001113545
VERSION     NM_001113545.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Li,S., Yang,F., Cheng,F., Zhu,L. and Yan,Y.
  TITLE     Lipotoxic hepatocyte derived LIMA1 enriched small extracellular
            vesicles promote hepatic stellate cells activation via inhibiting
            mitophagy
  JOURNAL   Cell Mol Biol Lett 29 (1), 82 (2024)
   PUBMED   38822260
  REMARK    GeneRIF: Lipotoxic hepatocyte derived LIMA1 enriched small
            extracellular vesicles promote hepatic stellate cells activation
            via inhibiting mitophagy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4198)
  AUTHORS   Duethorn,B., Groll,F., Rieger,B., Drexler,H.C.A., Brinkmann,H.,
            Kremer,L., Stehling,M., Borowski,M.T., Mildner,K., Zeuschner,D.,
            Zernicka-Goetz,M., Stemmler,M.P., Busch,K.B., Vaquerizas,J.M. and
            Bedzhov,I.
  TITLE     Lima1 mediates the pluripotency control of membrane dynamics and
            cellular metabolism
  JOURNAL   Nat Commun 13 (1), 610 (2022)
   PUBMED   35105859
  REMARK    GeneRIF: Lima1 mediates the pluripotency control of membrane
            dynamics and cellular metabolism.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4198)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4198)
  AUTHORS   Zhang,Y.Y., Fu,Z.Y., Wei,J., Qi,W., Baituola,G., Luo,J., Meng,Y.J.,
            Guo,S.Y., Yin,H., Jiang,S.Y., Li,Y.F., Miao,H.H., Liu,Y., Wang,Y.,
            Li,B.L., Ma,Y.T. and Song,B.L.
  TITLE     A LIMA1 variant promotes low plasma LDL cholesterol and decreases
            intestinal cholesterol absorption
  JOURNAL   Science 360 (6393), 1087-1092 (2018)
   PUBMED   29880681
  REMARK    GeneRIF: this study identifies LIMA1 as a key protein regulating
            intestinal cholesterol absorption.
REFERENCE   6  (bases 1 to 4198)
  AUTHORS   Leitner,L., Shaposhnikov,D., Descot,A., Hoffmann,R. and Posern,G.
  TITLE     Epithelial Protein Lost in Neoplasm alpha (Eplin-alpha) is
            transcriptionally regulated by G-actin and MAL/MRTF coactivators
  JOURNAL   Mol Cancer 9, 60 (2010)
   PUBMED   20236507
  REMARK    GeneRIF: Eplin-alpha transcription is regulated by actin-MAL-SRF
            signalling.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4198)
  AUTHORS   Abe,K. and Takeichi,M.
  TITLE     EPLIN mediates linkage of the cadherin catenin complex to F-actin
            and stabilizes the circumferential actin belt
  JOURNAL   Proc Natl Acad Sci U S A 105 (1), 13-19 (2008)
   PUBMED   18093941
  REMARK    GeneRIF: EPLIN functions to link the cadherin-catenin complex to
            F-actin and simultaneously stabilizes this population of actin
            fibers, resulting in the establishment of the adhesion
REFERENCE   8  (bases 1 to 4198)
  AUTHORS   Han,M.Y., Kosako,H., Watanabe,T. and Hattori,S.
  TITLE     Extracellular signal-regulated kinase/mitogen-activated protein
            kinase regulates actin organization and cell motility by
            phosphorylating the actin cross-linking protein EPLIN
  JOURNAL   Mol Cell Biol 27 (23), 8190-8204 (2007)
   PUBMED   17875928
  REMARK    GeneRIF: ERK-mediated phosphorylation of EPLIN contributes to actin
            filament reorganization and enhanced cell motility.
REFERENCE   9  (bases 1 to 4198)
  AUTHORS   Song,Y., Maul,R.S., Gerbin,C.S. and Chang,D.D.
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3
            cells by epithelial protein lost in neoplasm (EPLIN) requires
            localization of EPLIN to actin cytoskeleton
  JOURNAL   Mol Biol Cell 13 (4), 1408-1416 (2002)
   PUBMED   11950948
  REMARK    GeneRIF: EPLIN may function in growth control by associating with
            and regulating the actin cytoskeleton
REFERENCE   10 (bases 1 to 4198)
  AUTHORS   Maul,R.S., Sachi Gerbin,C. and Chang,D.D.
  TITLE     Characterization of mouse epithelial protein lost in neoplasm
            (EPLIN) and comparison of mammalian and zebrafish EPLIN
  JOURNAL   Gene 262 (1-2), 155-160 (2001)
   PUBMED   11179679
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138177.12 and AC134548.5.
            
            On Sep 13, 2022 this sequence version replaced NM_001113545.1.
            
            Transcript Variant: This variant (a) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK049350.1, BC031490.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-79                AC138177.12        125854-125932
            80-228              AC134548.5         175947-176095       c
            229-274             AC134548.5         159064-159109       c
            275-739             AC134548.5         151766-152230       c
            740-824             AC134548.5         139931-140015       c
            825-973             AC134548.5         138695-138843       c
            974-1075            AC134548.5         134383-134484       c
            1076-1133           AC134548.5         129014-129071       c
            1134-1243           AC134548.5         126681-126790       c
            1244-1377           AC134548.5         115792-115925       c
            1378-4198           AC134548.5         110742-113562       c
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 56.13 cM"
     gene            1..4198
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /note="LIM domain and actin binding 1"
                     /db_xref="GeneID:65970"
                     /db_xref="MGI:MGI:1920992"
     exon            1..79
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            80..228
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..2371
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /note="isoform a is encoded by transcript variant a; LIM
                     domain and actin-binding protein 1; epithelial protein
                     lost in neoplasm"
                     /codon_start=1
                     /product="LIM domain and actin-binding protein 1 isoform
                     a"
                     /protein_id="NP_001107017.1"
                     /db_xref="CCDS:CCDS49729.1"
                     /db_xref="GeneID:65970"
                     /db_xref="MGI:MGI:1920992"
                     /translation="MESTPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKA
                     AEEANMERKKNNPESLPQHFRRGTLSVLKKKWENPVAGAEFHTDSLPNSSSEGGHTAD
                     YPPAEVTDKPAPGVRADREEHTQPKPRFGSRPEAVIQSRYPRSENSHDFKAQATESQK
                     MENCLGDSRHEAEKPETSENTETSGKIEKYNVPLNRLKMMFEKGEHNQTKSLWTQSRN
                     AGGRRLSENNCSLDDWEIGAGHLSSSAFNSEKNESKRNLELPRLSETSIKDRMAKYQA
                     AVSKQSSPASYTNELKTSESKTHKWEQKENVPPGPEACSVHQEGSKVSTTENSLVALS
                     VPAEDDTCNSQVKSEAQQPMHPKPLSPDARTSSLPESSPSKTAKKFQAPAKESCVECQ
                     KTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNY
                     DEGFGHKQHKDLWASKSDNEETLGRPAQPPNAGESPHSPGVEDAPIAKVGVLAASMEA
                     KASSQREREDKPAETKKLRIAWPPPAELGGSGSALEEGIKVSKPKWPPEDDVCKTEAP
                     EDVDLDLKKLRRSSSLKERSRPFTVAASFRTSSIKSPKASSPSLRKGWSESEQSEEFG
                     GGIATMERKQTENARPSGEKENVGKSRWQGEEVPRSKDRSSFELESENFMENGANIAE
                     DDNHVHAQQSPLEPEAPGWSGFVDTTAAKEFTTQNQKSQDVGFWEGEVVRELSVEEQI
                     KRNRYYDEDEDEE"
     exon            229..274
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            275..739
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            740..824
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            825..973
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1075
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1133
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1243
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1377
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..4198
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4179..4184
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /note="hexamer: AATAAA"
     polyA_site      4198
                     /gene="Lima1"
                     /gene_synonym="Eplin"
                     /note="major polyA site"
ORIGIN      
        1 agttgcgaca ggtgcgctgg gcagacagag cgcaggaccg tggccgtgca gcgccgagcc
       61 ccgacggctt tctagtcagc ccacaggtgt ctcagagtct gtagacaaga tggaatcgac
      121 tccatttaat agacgccagt ggacttccct gtcattgaga gtaacagcca aagagctttc
      181 tcttgtcaac aagaacaagt catccgcaat tgtggaaata ttctccaagt accagaaggc
      241 tgctgaagaa gccaacatgg aaaggaagaa aaataaccct gaaagtctgc cccagcactt
      301 tagaagaggg accctgtctg tgttaaagaa gaagtgggag aacccggtgg ctggggcaga
      361 attccacaca gactcactgc caaacagcag cagtgagggt gggcacacag cggactaccc
      421 tcctgctgaa gtgacggaca agcctgctcc tggagtcaga gctgaccggg aagagcacac
      481 ccagcccaaa cctagatttg gatctcgtcc ggaagcagtt atccagagcc ggtatcctcg
      541 ctcagagaac agccacgatt ttaaagccca ggccacggag agccaaaaaa tggaaaactg
      601 tctgggagat tccaggcatg aagcagagaa gccagagacg agcgaaaaca cagaaacttc
      661 aggcaaaata gagaaataca acgttccact gaatagactg aagatgatgt ttgagaaagg
      721 tgaacacaac caaaccaaga gtctctggac ccaaagccga aatgcgggtg gaaggaggct
      781 ctctgaaaac aactgttccc tggatgactg ggaaataggt gcgggtcatt tgtcttcctc
      841 tgcattcaac tcggagaaaa acgagagtaa gaggaatctg gagctgccac gcctctcaga
      901 aacctccata aaggaccgca tggccaagta ccaggctgca gtgtccaagc agagcagccc
      961 agccagctac acaaatgagc tgaaaaccag tgaaagcaaa actcataaat gggaacagaa
     1021 ggagaatgtg cccccaggtc ccgaggcctg cagcgtccat caggaaggaa gcaaggtttc
     1081 tacaactgag aatagtctag tggccctctc tgtccctgct gaagatgaca cctgtaactc
     1141 ccaggtgaag agcgaggccc agcagcctat gcaccctaag ccgctgagtc ctgacgccag
     1201 gacctccagt cttccggaaa gttctccttc caaaacagcg aagaagtttc aggcgccggc
     1261 aaaggagagc tgcgtggagt gtcagaagac ggtgtacccc atggaacggc tcctggccaa
     1321 ccagcaggtg tttcacatca gctgtttccg atgctcctac tgcaacaaca agctcagtct
     1381 aggaacatat gcatccttac atggacgaat ctactgtaag cctcacttca atcaactctt
     1441 caagtccaaa ggcaactatg acgagggctt tgggcacaag cagcataagg atctgtgggc
     1501 aagcaagagc gacaatgagg agactttggg gagaccagcc cagccgccta atgcagggga
     1561 gagcccccat agcccgggtg tagaagatgc ccccatcgcc aaggtcggcg tgctggcggc
     1621 aagtatggaa gccaaggcct cttctcagag ggagagggag gataagcctg cggagaccaa
     1681 gaagctgagg atcgcctggc ctccgccagc cgagctgggc ggttccggaa gtgccctgga
     1741 ggaagggatc aaagtatcga agcccaagtg gcctccggag gatgacgtct gcaagacgga
     1801 ggccccggag gatgtagatc tggacctgaa gaagctgcgg cggtcttcgt ccctgaagga
     1861 gaggagccgc ccgtttacgg tagcagcttc ctttcgaacc tcttccatca agagccccaa
     1921 ggcctcttcg ccatctctca ggaaaggttg gagcgagtcc gagcagagtg aggagtttgg
     1981 aggagggata gcgacgatgg aaaggaaaca aacggaaaat gccagaccct ctggggagaa
     2041 agagaatgtg ggaaaatcac gctggcaggg cgaggaagtg ccgaggagca aggaccgtag
     2101 ttcttttgag ctggagagtg agaattttat ggaaaatgga gcaaacatag ctgaagatga
     2161 caaccatgtc cacgcacagc agtccccgtt agaacccgag gctccgggtt ggtctggctt
     2221 tgtagacacc acggctgcta aagaattcac tacccagaat caaaaatccc aagatgtcgg
     2281 gttctgggag ggagaagtgg tccgagagct gtctgtggag gaacagataa agagaaaccg
     2341 gtactatgat gaggacgagg atgaagaatg accggccgcc atggttctgg ccttagattt
     2401 tggttagcaa ctcactgccc tttatcaagt ggtgcgcaga aacaggcagc ttggcgccaa
     2461 gggtcattca tgtgggagca actttggaaa tgaattcccg ctagcaaaac agggcaaaaa
     2521 caagcaaaaa caaaacagac aaacaaactc acccaaaacc aattctaagc gcattgtcaa
     2581 aacaccagtg atcttttaag cctggtgtgg tggtaaacac ctttaatccc agcactccac
     2641 tcgggaggca gaggcagagg caggtgggtc tctctgagtt ccaggacagc cagggctaca
     2701 aagagaaacc ttgcctcaaa acaaacaaac aaacaaatcc ctccattttt tcagggaagg
     2761 gctaagtagg gatttctctc actgatgctg cctgccatcc actgcattcc ccaaatcaag
     2821 attggtgtgg ttagatattt agaggcttgt tttcacacta cagagaccat tgtggaatta
     2881 gagacacaca caacacattt aggagcccac ctcatgacca gaggcacttt agtataaagg
     2941 gcacagtgta gctattttac caatttcatt ttttagtact caattgctga aaatcaaata
     3001 tttagtcttt caagcttttg aaatgcattc tcttcctctg gtagataggg aaagcttact
     3061 gtctttcatc ctacatcctg tatttttaat cactgaggca caaaacagca acctttggcc
     3121 aagtgaaagg cgctggttac tgaagccgga gcagcagtgt tcaatcccca aaacctacgt
     3181 aaaggtgaga gagcagactg cacagagctg tccacacgtg cccatcatca acaataagaa
     3241 acaagttaaa aaaaaaaata caaccaacct cacaaacacc tgcgagacag agaaccaacc
     3301 tatatacttt ggtgtttgga gagacggaca ttgtacccag ataatcagtg gcgaatgcca
     3361 gtgtgtctcc ctgcactctg ggtgtttgcc atctcttctg gtggtttctc gcactttgcg
     3421 gaaagctgca gtgttgctgt aagacctttt ggatcacgct tcaccatgct ccaagtaaaa
     3481 caacctgaga tcagagctgg gaagcctcag gtcgaggcga atcttggctc tgaaatgtat
     3541 tgatttttag gccagataat tcttccaggg ttgcattaaa tcacttttag gtctgtcttc
     3601 ttaggccgtg tcctactttg attcattgta gtgataaaac actgggcagg agcaactcag
     3661 aggggaaggg tttgtctcca ctcacaggcc acgggtcaca cacaggccac gcgcgcacgc
     3721 agcatggtca agggaagcga ggccagaacc cagaggcaag agctgatgca gaccgtgtgg
     3781 gaacactgct tgctgacttc atctctgtgg cttactcaac tggttttctt acaccaccca
     3841 gggccacctg gccaggggtg acactgctca caacaaaaac agacaaggcc cacaggtgca
     3901 tcggttagag gcggttcatc agctgagtcc cacatccagg tgactctgtg tgaggctggt
     3961 aaacccagag gaggatagat ggttttctct ggtattaagg ctttagaata ttataaacga
     4021 gtgcagttct tgtatgtaac tgatattcca aatgatgtgt atggcggggt gggcttaagg
     4081 tagtggcctc tttgcatttg tagagattta gctcagtttt agagatgtaa aataatctgt
     4141 ttccttatgg ccttgtttag cctaaaacat ttttattcaa taaagctttt aatttaca
//
