LOCUS       NM_177693                886 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus lens intrinsic membrane protein 2 (Lim2), mRNA.
ACCESSION   NM_177693
VERSION     NM_177693.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 886)
  AUTHORS   Shi,Y., De Maria,A.B., Wang,H., Mathias,R.T., FitzGerald,P.G. and
            Bassnett,S.
  TITLE     Further analysis of the lens phenotype in Lim2-deficient mice
  JOURNAL   Invest Ophthalmol Vis Sci 52 (10), 7332-7339 (2011)
   PUBMED   21775657
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 886)
  AUTHORS   Luu,B., Ellisor,D. and Zervas,M.
  TITLE     The lineage contribution and role of Gbx2 in spinal cord
            development
  JOURNAL   PLoS One 6 (6), e20940 (2011)
   PUBMED   21698205
REFERENCE   4  (bases 1 to 886)
  AUTHORS   Puk,O., Ahmad,N., Wagner,S., de Angelis,M.H. and Graw,J.
  TITLE     Microphakia and congenital cataract formation in a novel Lim2(C51R)
            mutant mouse
  JOURNAL   Mol Vis 17, 1164-1171 (2011)
   PUBMED   21617753
  REMARK    GeneRIF: A novel missense mutation in the Lim2 gene affects lens
            development in a semidominant manner. Since homozygous mutants
            develop congenital lens opacities, this mouse line can be used as a
            model for inherited cataract formation in humans.
REFERENCE   5  (bases 1 to 886)
  AUTHORS   Shi,Y., Barton,K., De Maria,A., Petrash,J.M., Shiels,A. and
            Bassnett,S.
  TITLE     The stratified syncytium of the vertebrate lens
  JOURNAL   J Cell Sci 122 (Pt 10), 1607-1615 (2009)
   PUBMED   19401333
REFERENCE   6  (bases 1 to 886)
  AUTHORS   Kerscher,S., Glenister,P.H., Favor,J. and Lyon,M.F.
  TITLE     Two new cataract loci, Ccw and To3, and further mapping of the Npp
            and Opj cataracts in the mouse
  JOURNAL   Genomics 36 (1), 17-21 (1996)
   PUBMED   8812411
REFERENCE   7  (bases 1 to 886)
  AUTHORS   Kerscher,S., Church,R.L., Boyd,Y. and Lyon,M.F.
  TITLE     Mapping of four mouse genes encoding eye lens-specific structural,
            gap junction, and integral membrane proteins: Cryba1 (crystallin
            beta A3/A1), Crybb2 (crystallin beta B2), Gja8 (MP70), and Lim2
            (MP19)
  JOURNAL   Genomics 29 (2), 445-450 (1995)
   PUBMED   8666393
REFERENCE   8  (bases 1 to 886)
  AUTHORS   Favor,J.
  TITLE     Characterization of dominant cataract mutations in mice:
            penetrance, fertility and homozygous viability of mutations
            recovered after 250 mg/kg ethylnitrosourea paternal treatment
  JOURNAL   Genet Res 44 (2), 183-197 (1984)
   PUBMED   6510713
REFERENCE   9  (bases 1 to 886)
  AUTHORS   Favor,J.
  TITLE     A comparison of the dominant cataract and recessive specific-locus
            mutation rates induced by treatment of male mice with
            ethylnitrosourea
  JOURNAL   Mutat Res 110 (2), 367-382 (1983)
   PUBMED   6877261
REFERENCE   10 (bases 1 to 886)
  AUTHORS   Ehling,U.H., Favor,J., Kratochvilova,J. and Neuhauser-Klaus,A.
  TITLE     Dominant cataract mutations and specific-locus mutations in mice
            induced by radiation or ethylnitrosourea
  JOURNAL   Mutat Res 92 (1-2), 181-192 (1982)
   PUBMED   7088001
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CK780500.1, AK029354.1 and CX734633.1.
            
            On Jun 9, 2018 this sequence version replaced NM_177693.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092386.1, AK029354.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 CK780500.1         12-13
            3-868               AK029354.1         2-867
            869-886             CX734633.1         221-238
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 28.25 cM"
     gene            1..886
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /note="lens intrinsic membrane protein 2"
                     /db_xref="GeneID:233187"
                     /db_xref="MGI:MGI:104698"
     exon            1..58
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /inference="alignment:Splign:2.1.0"
     exon            59..239
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..586
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /note="total opacity 3"
                     /codon_start=1
                     /product="lens fiber membrane intrinsic protein"
                     /protein_id="NP_808361.1"
                     /db_xref="CCDS:CCDS21168.1"
                     /db_xref="GeneID:233187"
                     /db_xref="MGI:MGI:104698"
                     /translation="MYSFMGGGLFCAWVGTILLVVATATDHWMQYRLSGSFAHQGLWR
                     YCLGNKCFLQTESIAYWNATRAFMILSALCATSGIIMGVLAFAQQSTFTRLSRPFSAG
                     IMFFASTLFVLLALAIYTGVTVSFLGRRFGDWRFSWSYILGWVALLMTFFAGIFYMCA
                     YRMHECRRLATPR"
     exon            240..389
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /inference="alignment:Splign:2.1.0"
     exon            390..524
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /inference="alignment:Splign:2.1.0"
     exon            525..873
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      848..853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /note="hexamer: AATAAA"
     polyA_site      868
                     /gene="Lim2"
                     /gene_synonym="4833403J20; MP17; MP18; MP19; MP20; To3"
                     /note="major polyA site"
ORIGIN      
        1 cagtatccac agaggggctg tggcagaagg agggctcaga acagactctg ccactcagat
       61 caccatgtac agcttcatgg gtggcggcct cttctgtgcc tgggtgggga ccatcctgtt
      121 ggtggtagcc acagcaactg accactggat gcagtaccgg ctgtcggggt ccttcgcaca
      181 ccagggcctg tggcgttact gcctgggcaa caagtgcttc ctgcagaccg agagcatcgc
      241 atattggaat gccacccggg ctttcatgat cctgtctgcc ctgtgtgcca cctcgggcat
      301 catcatgggt gtcctagcct ttgcgcagca atccaccttc acccgtctct ccaggccctt
      361 ctctgccggc atcatgtttt ttgcctccac cctttttgtc ctgttggcct tggctattta
      421 cactggagtc accgtcagtt tcctcggccg ccgctttgga gactggcgct tttcatggtc
      481 ttacatcctg ggctgggtgg ccctgctcat gaccttcttt gcaggaattt tctacatgtg
      541 tgcctaccgg atgcatgagt gccggcgcct agccacccca cgctgagccc tggggtaaaa
      601 cttgcagctg gaagttacag tgagggcact gaagaggaag gggaggacct agaggccgga
      661 agtcctggct cctgagggaa tgagggggct cagtcctgga ttctagatag ggagcccctg
      721 atccctctgt cctgtgtggg gaggaaaagt agggtcaagt gggagggagt tgagaagaca
      781 taagccccca cctgccctga ggggcggtag aaaatggaag ctcctttaga gagacatttg
      841 gtgatctaat aaaactgatg tgaaactgtc aaaaaaaaaa aaaaaa
//
