LOCUS       NM_176953               4069 bp    mRNA    linear   ROD 23-APR-2024
DEFINITION  Mus musculus ligase IV, DNA, ATP-dependent (Lig4), transcript
            variant 1, mRNA.
ACCESSION   NM_176953
VERSION     NM_176953.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Kumari,N., Antil,H., Kumari,S. and Raghavan,S.C.
  TITLE     Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA
            damage, and can sensitize cells to cancer therapeutics
  JOURNAL   Genomics 115 (6), 110731 (2023)
   PUBMED   37871849
  REMARK    GeneRIF: Deficiency of ligase IV leads to reduced NHEJ,
            accumulation of DNA damage, and can sensitize cells to cancer
            therapeutics.
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Lun,M.P., Shannon,M.L., Keles,S., Reisli,I., Luche,N., Ryan,D.,
            Capuder,K., Notarangelo,L.D. and Lehtinen,M.K.
  TITLE     Spatiotemporal Gradient of Cortical Neuron Death Contributes to
            Microcephaly in Knock-In Mouse Model of Ligase 4 Syndrome
  JOURNAL   Am J Pathol 189 (12), 2440-2449 (2019)
   PUBMED   31541646
  REMARK    GeneRIF: Hypomorphic Lig4 mutation Promotes nonprogressive
            microcephaly.
REFERENCE   3  (bases 1 to 4069)
  AUTHORS   Canela,A., Maman,Y., Huang,S.N., Wutz,G., Tang,W.,
            Zagnoli-Vieira,G., Callen,E., Wong,N., Day,A., Peters,J.M.,
            Caldecott,K.W., Pommier,Y. and Nussenzweig,A.
  TITLE     Topoisomerase II-Induced Chromosome Breakage and Translocation Is
            Determined by Chromosome Architecture and Transcriptional Activity
  JOURNAL   Mol Cell 75 (2), 252-266 (2019)
   PUBMED   31202577
REFERENCE   4  (bases 1 to 4069)
  AUTHORS   Mirman,Z., Lottersberger,F., Takai,H., Kibe,T., Gong,Y., Takai,K.,
            Bianchi,A., Zimmermann,M., Durocher,D. and de Lange,T.
  TITLE     53BP1-RIF1-shieldin counteracts DSB resection through CST- and
            Polalpha-dependent fill-in
  JOURNAL   Nature 560 (7716), 112-116 (2018)
   PUBMED   30022158
REFERENCE   5  (bases 1 to 4069)
  AUTHORS   Kim,J., Choi,I. and Lee,Y.
  TITLE     Involvement of Atm and Trp53 in neural cell loss due to Terf2
            inactivation during mouse brain development
  JOURNAL   Histochem Cell Biol 148 (5), 489-501 (2017)
   PUBMED   28620865
REFERENCE   6  (bases 1 to 4069)
  AUTHORS   Gu,Y., Sekiguchi,J., Gao,Y., Dikkes,P., Frank,K., Ferguson,D.,
            Hasty,P., Chun,J. and Alt,F.W.
  TITLE     Defective embryonic neurogenesis in Ku-deficient but not
            DNA-dependent protein kinase catalytic subunit-deficient mice
  JOURNAL   Proc Natl Acad Sci U S A 97 (6), 2668-2673 (2000)
   PUBMED   10716994
REFERENCE   7  (bases 1 to 4069)
  AUTHORS   Karanjawala,Z.E., Grawunder,U., Hsieh,C.L. and Lieber,M.R.
  TITLE     The nonhomologous DNA end joining pathway is important for
            chromosome stability in primary fibroblasts
  JOURNAL   Curr Biol 9 (24), 1501-1504 (1999)
   PUBMED   10607596
REFERENCE   8  (bases 1 to 4069)
  AUTHORS   Gao,Y., Sun,Y., Frank,K.M., Dikkes,P., Fujiwara,Y., Seidl,K.J.,
            Sekiguchi,J.M., Rathbun,G.A., Swat,W., Wang,J., Bronson,R.T.,
            Malynn,B.A., Bryans,M., Zhu,C., Chaudhuri,J., Davidson,L.,
            Ferrini,R., Stamato,T., Orkin,S.H., Greenberg,M.E. and Alt,F.W.
  TITLE     A critical role for DNA end-joining proteins in both lymphogenesis
            and neurogenesis
  JOURNAL   Cell 95 (7), 891-902 (1998)
   PUBMED   9875844
REFERENCE   9  (bases 1 to 4069)
  AUTHORS   Barnes,D.E., Stamp,G., Rosewell,I., Denzel,A. and Lindahl,T.
  TITLE     Targeted disruption of the gene encoding DNA ligase IV leads to
            lethality in embryonic mice
  JOURNAL   Curr Biol 8 (25), 1395-1398 (1998)
   PUBMED   9889105
REFERENCE   10 (bases 1 to 4069)
  AUTHORS   Frank,K.M., Sekiguchi,J.M., Seidl,K.J., Swat,W., Rathbun,G.A.,
            Cheng,H.L., Davidson,L., Kangaloo,L. and Alt,F.W.
  TITLE     Late embryonic lethality and impaired V(D)J recombination in mice
            lacking DNA ligase IV
  JOURNAL   Nature 396 (6707), 173-177 (1998)
   PUBMED   9823897
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ088420.1, AK030966.1 and AC138397.4.
            
            On Dec 10, 2019 this sequence version replaced NM_176953.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148081.1, AK083353.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-269               CJ088420.1         91-359
            270-802             AK030966.1         198-730
            803-4069            AC138397.4         145152-148418
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 4.52 cM"
     gene            1..4069
                     /gene="Lig4"
                     /gene_synonym="5830471N16Rik; tiny"
                     /note="ligase IV, DNA, ATP-dependent"
                     /db_xref="GeneID:319583"
                     /db_xref="MGI:MGI:1335098"
     exon            1..281
                     /gene="Lig4"
                     /gene_synonym="5830471N16Rik; tiny"
                     /inference="alignment:Splign:2.1.0"
     exon            282..4069
                     /gene="Lig4"
                     /gene_synonym="5830471N16Rik; tiny"
                     /inference="alignment:Splign:2.1.0"
     CDS             311..3046
                     /gene="Lig4"
                     /gene_synonym="5830471N16Rik; tiny"
                     /EC_number="6.5.1.1"
                     /note="polydeoxyribonucleotide synthase [ATP] 4; DNA
                     ligase IV"
                     /codon_start=1
                     /product="DNA ligase 4"
                     /protein_id="NP_795927.2"
                     /db_xref="CCDS:CCDS22092.1"
                     /db_xref="GeneID:319583"
                     /db_xref="MGI:MGI:1335098"
                     /translation="MASSQTSQTVAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLD
                     SWRKFHDALHKNRKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRE
                     GKDAQKLLNYRTPSGARTDAGDFAMIAYFVLKPRCLQKGSLTIQQVNELLDLVASNNS
                     GKKKDLVKKSLLQLITQSSALEQKWLIRMIIKDLKLGISQQTIFSIFHNDAVELHNVT
                     TDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAVADVERVEKDMKQQSFYIETKLDG
                     ERMQMHKDGALYRYFSRNGYNYTDQFGESPQEGSLTPFIHNAFGTDVQACILDGEMMA
                     YNPTTQTFMQKGVKFDIKRMVEDSGLQTCYSVFDVLMVNKKKLGRETLRKRYEILSST
                     FTPIQGRIEIVQKTQAHTKKEVVDALNDAIDKREEGIMVKHPLSIYKPDKRGEGWLKI
                     KPEYVSGLMDELDVLIVGGYWGKGSRGGMMSHFLCAVAETPPPGDRPSVFHTLCRVGS
                     GYTMKELYDLGLKLAKYWKPFHKKSPPSSILCGTEKPEVYIEPQNSVIVQIKAAEIVP
                     SDMYKTGSTLRFPRIEKIRDDKEWHECMTLGDLEQLRGKASGKLATKHLHVGDDDEPR
                     EKRRKPISKTKKAIRIIEHLKAPNLSNVNKVSNVFEDVEFCVMSGLDGYPKADLENRI
                     AEFGGYIVQNPGPDTYCVIAGSENVRVKNIISSDKNDVVKPEWLLECFKTKTCVPWQP
                     RFMIHMCPSTKQHFAREYDCYGDSYFVDTDLDQLKEVFLGIKPSEQQTPEEMAPVIAD
                     LECRYSWDHSPLSMFRHYTIYLDLYAVINDLSSRIEATRLGITALELRFHGAKVVSCL
                     SEGVSHVIIGEDQRRVTDFKIFRRMLKKKFKILQESWVSDSVDKGELQEENQYLL"
     regulatory      4043..4048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lig4"
                     /gene_synonym="5830471N16Rik; tiny"
                     /note="hexamer: AATAAA"
     polyA_site      4069
                     /gene="Lig4"
                     /gene_synonym="5830471N16Rik; tiny"
                     /note="major polyA site"
ORIGIN      
        1 gctaagcaag tggtgtggcg ggttggagga ggcagggagg ttcttggtcc tagggaggct
       61 gcgtccttcc cgtgctttgt cccagcgtca ccacccgcct tccacggtgc agccggctcg
      121 ctggggactg atttcaggtg gcagccaccg ggtgaagaaa tcgtgtcctg atgcttagtt
      181 gtatccgcac gctgccctgc cccctgccac ctagctacct gggacctgcg cgctccccca
      241 gctccttcat tcggcagggt tggggttcac gggctgctta gttcaaaccg gaggatttgt
      301 tgtcgcttct atggcttcct cacaaacttc acaaactgtt gcagctcatg tcccctttgc
      361 agacttatgt tccacactag aacggataca gaaaggtaaa gaccgtgcag aaaaaatcag
      421 gcacttcaag gagtttttgg attcgtggag aaaatttcat gatgcccttc ataagaacag
      481 aaaggacgtt acagactctt tttaccctgc aatgagactc attctccccc agttagaaag
      541 agagaggatg gcttatggaa tcaaagaaac catgctcgct aagctctaca tcgaattgct
      601 gaacttacca cgagaaggca aggatgccca gaagctcctc aattaccgaa cccccagtgg
      661 agctcgcacg gatgctgggg actttgccat gattgcatac tttgttttga agccaaggtg
      721 cttacagaaa ggaagcttaa ccatacagca ggtaaatgaa ctcttagact tagttgccag
      781 caataactct ggcaaaaaaa aagacctagt gaaaaagagc cttcttcagt taataaccca
      841 gagttcagca ctggagcaaa aatggctgat tcgcatgatt atcaaagact taaagcttgg
      901 catcagtcag caaacaatat tttccatttt ccacaatgat gcagttgagt tgcacaacgt
      961 caccacagat ctggaaaagg tctgcaggca gctgcatgac ccctctgtag ggcttagtga
     1021 catctctatc actctgtttt ctgcctttaa gccaatgcta gctgctgtag cagacgtgga
     1081 gcgtgtggag aaggacatga agcagcagag tttctacatc gaaactaagc ttgatggtga
     1141 gcgcatgcag atgcacaaag atggcgcgct gtaccggtac ttctccagaa acggttacaa
     1201 ctataccgac cagtttggtg aatctccaca ggaaggctct ctcaccccat ttattcacaa
     1261 tgcgttcggg acagatgtgc aagcgtgcat ccttgacggt gagatgatgg cctacaaccc
     1321 aacaacacag actttcatgc agaagggggt caagtttgat atcaaaagga tggtggaaga
     1381 ttctggccta cagacttgtt actctgtttt tgacgtactg atggttaata agaagaagct
     1441 agggcgcgag actcttagga agaggtatga gatccttagt agcactttca cacccataca
     1501 aggccgcata gaaatagtgc agaaaactca agctcataca aagaaggaag tagtggatgc
     1561 attaaatgat gccatagaca agagagaaga ggggatcatg gttaaacacc ctctgtccat
     1621 ttacaagcca gacaaaagag gtgaagggtg gctaaagatt aaaccagagt acgtcagtgg
     1681 actaatggat gaattagacg tcctaattgt ggggggctac tggggtaaag gttcacgagg
     1741 tggcatgatg tctcactttt tgtgtgcagt ggcagagaca ccacctcctg gtgacaggcc
     1801 atctgtattc cacactctgt gccgtgttgg gtctggttac accatgaaag aactctatga
     1861 cctgggcttg aaattggcaa aatactggaa gccttttcat aagaaatctc caccaagtag
     1921 cattctgtgt ggtacagaga agccggaagt gtacatagag cctcagaact ctgtcattgt
     1981 tcagatcaag gcagctgaga tcgtccccag tgacatgtac aagactggct ccaccctgcg
     2041 cttcccacgc atcgaaaaga tcagagatga caaagagtgg cacgaatgta tgacactggg
     2101 tgacttggag cagctgaggg ggaaagcatc tgggaagctt gccacgaaac accttcatgt
     2161 aggtgatgat gatgaaccta gagaaaaaag gcggaagccc atctccaaga cgaagaaagc
     2221 cattagaatc attgaacact taaaagcacc caacctttct aacgtaaaca aagtttccaa
     2281 tgtatttgaa gatgttgagt tttgtgttat gagtggatta gatggttatc caaaggctga
     2341 cctagagaac agaattgcag aattcggtgg ttatatagta cagaatccag gcccggatac
     2401 atactgtgtt attgcaggtt ctgagaacgt tagagtgaaa aacattattt cttcagataa
     2461 aaatgatgtt gtcaagcccg agtggctttt agagtgtttt aagacaaaaa catgcgtgcc
     2521 gtggcaacct cgctttatga ttcacatgtg cccgtcgaca aagcagcatt ttgcccgtga
     2581 gtatgactgc tatggtgata gctattttgt tgacacagat ttggatcaat tgaaagaagt
     2641 gtttctagga attaaaccca gtgagcagca gactcctgaa gaaatggccc ctgtgattgc
     2701 tgacttagaa tgtcgttatt cctgggacca ctctcctctc agtatgtttc gacattacac
     2761 catttatttg gacttgtatg ctgttattaa tgacttgagt tccagaattg aagccacgag
     2821 attaggtatt acagcccttg agctgcggtt tcatggagca aaggtggttt cctgcttatc
     2881 tgaaggggta tctcatgtta tcattgggga ggatcagaga cgagttactg actttaaaat
     2941 attcagaaga atgcttaaga aaaagtttaa aatcctgcaa gaaagttggg tgtccgattc
     3001 agtagacaag ggcgaactgc aggaggaaaa ccagtatttg ctttagagct tgctggcccg
     3061 gcgggggggg gggaagaaga agtgttattg tcagacaaca gcagacagtg atgatgaaat
     3121 agccagtagc cttcattgta tctcctaata cttcaaaagg attattgatc agaacgcaat
     3181 aattttcaag tagaaaggaa actaaatggg ccaaagcaaa aaaaaaaaaa ccaaaaaaaa
     3241 taaaacaaac aaaaaaaacc aacccttttc ctaacacgac ttataactat ggcttcaaga
     3301 agtgcttgta tggtatccat actgaagcta tccatactta aagtgtattt gaaacctttt
     3361 agaaacatca ccactattga ctttctagac aaaattttcc agacttaaaa attaaagttt
     3421 ttagctaata aaagtattat accaataaaa aaaatcagaa ttgttaaagt tagacttata
     3481 aaaataagca tttttatttg cgggctcaaa taccccctat aagcatgaca tattaagcat
     3541 aaagagtttt taaggttcat caaatgtttt ttgataatgt gggggaagtt gagagagaat
     3601 catctaaaca ccaaacactt aagatattaa ttttattgaa aaaaatcctt gcaactttta
     3661 agttagctga gacttaattt tggtgaagac tttaatgttt catggagaaa tgtttgagtg
     3721 aatttacact ccagcattga cagtgtttac agaacttgtc tcctatcgat gatccattgc
     3781 gtagctgtcc atacagtgaa cactttcatt agagactggg tgctctggct tttagagatg
     3841 gcttttatga ctatgttgaa gtcacagtca tgtgtggatt gtatctgttg agaattgtac
     3901 ttccatggca gtactgaaat ggagggatgt gggaccttaa gtgatgggta taaactattt
     3961 aagtaattag acttgttgaa gtgattgaag aaacatttgt gattattgta acttaaataa
     4021 aattattaaa tattgtaact taaataaaat tattgaatat ttaatgtaa
//
