LOCUS       NM_001403857            6168 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus ligase III, DNA, ATP-dependent (Lig3), transcript
            variant 6, mRNA.
ACCESSION   NM_001403857 XM_006532350
VERSION     NM_001403857.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Caracciolo,D., Juli,G., Riillo,C., Coricello,A., Vasile,F.,
            Pollastri,S., Rocca,R., Scionti,F., Polera,N., Grillone,K.,
            Arbitrio,M., Staropoli,N., Caparello,B., Britti,D., Loprete,G.,
            Costa,G., Di Martino,M.T., Alcaro,S., Tagliaferri,P. and Tassone,P.
  TITLE     Exploiting DNA Ligase III addiction of multiple myeloma by
            flavonoid Rhamnetin
  JOURNAL   J Transl Med 20 (1), 482 (2022)
   PUBMED   36273153
  REMARK    GeneRIF: Exploiting DNA Ligase III addiction of multiple myeloma by
            flavonoid Rhamnetin.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Paes Dias,M., Tripathi,V., van der Heijden,I., Cong,K.,
            Manolika,E.M., Bhin,J., Gogola,E., Galanos,P., Annunziato,S.,
            Lieftink,C., Andujar-Sanchez,M., Chakrabarty,S., Smith,G.C.M., van
            de Ven,M., Beijersbergen,R.L., Bartkova,J., Rottenberg,S.,
            Cantor,S., Bartek,J., Ray Chaudhuri,A. and Jonkers,J.
  TITLE     Loss of nuclear DNA ligase III reverts PARP inhibitor resistance in
            BRCA1/53BP1 double-deficient cells by exposing ssDNA gaps
  JOURNAL   Mol Cell 81 (22), 4692-4708 (2021)
   PUBMED   34555355
  REMARK    GeneRIF: Loss of nuclear DNA ligase III reverts PARP inhibitor
            resistance in BRCA1/53BP1 double-deficient cells by exposing ssDNA
            gaps.
REFERENCE   3  (bases 1 to 6168)
  AUTHORS   Doksani,Y. and de Lange,T.
  TITLE     Telomere-Internal Double-Strand Breaks Are Repaired by Homologous
            Recombination and PARP1/Lig3-Dependent End-Joining
  JOURNAL   Cell Rep 17 (6), 1646-1656 (2016)
   PUBMED   27806302
  REMARK    GeneRIF: Telomere-internal double-strand breaks (DSBs) are also
            repaired by a PARP1- and Ligase3-dependent reaction, suggesting
            alternative non-homologous end-joining (alt-NHEJ), which relies on
            microhomology at DSBs.
REFERENCE   4  (bases 1 to 6168)
  AUTHORS   Katyal,S., Lee,Y., Nitiss,K.C., Downing,S.M., Li,Y., Shimada,M.,
            Zhao,J., Russell,H.R., Petrini,J.H., Nitiss,J.L. and McKinnon,P.J.
  TITLE     Aberrant topoisomerase-1 DNA lesions are pathogenic in
            neurodegenerative genome instability syndromes
  JOURNAL   Nat Neurosci 17 (6), 813-821 (2014)
   PUBMED   24793032
REFERENCE   5  (bases 1 to 6168)
  AUTHORS   Akbari,M., Keijzers,G., Maynard,S., Scheibye-Knudsen,M., Desler,C.,
            Hickson,I.D. and Bohr,V.A.
  TITLE     Overexpression of DNA ligase III in mitochondria protects cells
            against oxidative stress and improves mitochondrial DNA base
            excision repair
  JOURNAL   DNA Repair (Amst) 16, 44-53 (2014)
   PUBMED   24674627
  REMARK    GeneRIF: Results show that overexpression of DNA ligase III in
            mitochondria improves mitochondrial base excision repair and
            enhances cell survival after oxidative stress.
REFERENCE   6  (bases 1 to 6168)
  AUTHORS   Perez-Jannotti,R.M., Klein,S.M. and Bogenhagen,D.F.
  TITLE     Two forms of mitochondrial DNA ligase III are produced in Xenopus
            laevis oocytes
  JOURNAL   J Biol Chem 276 (52), 48978-48987 (2001)
   PUBMED   11598119
REFERENCE   7  (bases 1 to 6168)
  AUTHORS   Cohen-Tannoudji,M., Vandormael-Pournin,S., Le Bras,S.,
            Coumailleau,F., Babinet,C. and Baldacci,P.
  TITLE     A 2-Mb YAC/BAC-based physical map of the ovum mutant (Om) locus
            region on mouse chromosome 11
  JOURNAL   Genomics 68 (3), 273-282 (2000)
   PUBMED   10995569
REFERENCE   8  (bases 1 to 6168)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
REFERENCE   9  (bases 1 to 6168)
  AUTHORS   Tomkinson,A.E. and Levin,D.S.
  TITLE     Mammalian DNA ligases
  JOURNAL   Bioessays 19 (10), 893-901 (1997)
   PUBMED   9363683
  REMARK    Review article
REFERENCE   10 (bases 1 to 6168)
  AUTHORS   Mackey,Z.B., Ramos,W., Levin,D.S., Walter,C.A., McCarrey,J.R. and
            Tomkinson,A.E.
  TITLE     An alternative splicing event which occurs in mouse pachytene
            spermatocytes generates a form of DNA ligase III with distinct
            biochemical properties that may function in meiotic recombination
  JOURNAL   Mol Cell Biol 17 (2), 989-998 (1997)
   PUBMED   9001252
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645594.9.
            
            On Apr 5, 2022 this sequence version replaced XM_006532350.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1396934.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-365               AL645594.9         102090-102454
            366-919             AL645594.9         104023-104576
            920-1063            AL645594.9         105992-106135
            1064-1264           AL645594.9         108305-108505
            1265-1416           AL645594.9         109441-109592
            1417-1583           AL645594.9         110224-110390
            1584-1661           AL645594.9         110494-110571
            1662-1830           AL645594.9         111150-111318
            1831-1986           AL645594.9         112714-112869
            1987-2118           AL645594.9         114551-114682
            2119-2198           AL645594.9         115181-115260
            2199-2286           AL645594.9         116042-116129
            2287-2364           AL645594.9         116386-116463
            2365-2488           AL645594.9         116705-116828
            2489-2631           AL645594.9         117983-118125
            2632-2706           AL645594.9         118451-118525
            2707-2853           AL645594.9         118933-119079
            2854-3052           AL645594.9         120099-120297
            3053-3174           AL645594.9         120853-120974
            3175-6168           AL645594.9         121958-124951
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 50.23 cM"
     gene            1..6168
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /note="ligase III, DNA, ATP-dependent"
                     /db_xref="GeneID:16882"
                     /db_xref="MGI:MGI:109152"
     exon            1..365
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            366..919
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     CDS             370..3408
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /EC_number="6.5.1.1"
                     /note="isoform a is encoded by transcript variant 6; DNA
                     ligase (ATP) 3; DNA ligase III; polydeoxyribonucleotide
                     synthase [ATP] 3"
                     /codon_start=1
                     /product="DNA ligase 3 isoform a"
                     /protein_id="NP_001390786.1"
                     /db_xref="GeneID:16882"
                     /db_xref="MGI:MGI:109152"
                     /translation="MTLAFKILFPRNLCALGRKELCLFPEQNRWAAISQFSQWSETNL
                     LGGCCLLQRRKPVLALQRGHLRPRATHLTFWPGSHVGLCTGPCAMAEQRFCVDYAKRG
                     TAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIED
                     LTELEGWEELEDNEKEQISQHIADLSSKAAATPKKKAAVQAKLTTTGQVTSPVKGASF
                     ITSTNPRKFSGFSAAKPNNSEQAPSSPAPGTSLSASKCDPKHKDCLLREFRKLCAMVA
                     ENPSYNTKTQIIHDFLQKGSTGDGFHGDVYLTVKLLLPGVIKSVYNLNDKQIVKLFSR
                     IFNCNPDDMARDLEQGDVSETIRIFFEQSKSFPPAAKSLLTIQEVDAFLLHLSKLTKE
                     DEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQ
                     DVVERVLHNEQEVEKDPGRRRALRVQASLMTPVQPMLAEACKSIEYAMKKCPNGMFSE
                     IKYDGERVQVHKKGDHFSYFSRSLKPVLPHKVAHFKDYIPKAFPGGQSMILDSEVLLI
                     DNNTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMV
                     EIRNRIMFSEMKQVTKASDLADMINRVIREGLEGLVLKDVKGTYEPGKRHWLKVKKDY
                     LNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPDSQKWCTVTKCAGGHDDATL
                     ARLQKELVMVKISKDPSKIPSWLKINKIYYPDFIVPDPKKAAVWEITGAEFSRSEAHT
                     ADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKDKADFAVVAGDEASPTTGGSS
                     GENEGTAGSAGPCKGPPSKSSASAKTTEQKLNSPSSRGGIKPIPKHSPMKPGEKLAVK
                     SSPVKVGMKRKATDETPCLKKVLLDVFTGVRLYLPPSTPDFKRLKRYFVAFDGDLVQE
                     FDMGSATHVLGNREKNTDAQLVSSEWIWACIRKRRLIAPC"
     exon            920..1063
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1264
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1416
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1583
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1661
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1830
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..1986
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2118
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2198
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2286
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2364
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..2488
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2631
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            2632..2706
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2853
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..3052
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            3053..3174
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     exon            3175..6168
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6148..6153
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /note="hexamer: AATAAA"
     polyA_site      6168
                     /gene="Lig3"
                     /gene_synonym="D11Wsu78e"
                     /note="major polyA site"
ORIGIN      
        1 gtggcgcccc cacgggagcc cacgggaggc ccgcggcccc cgggaccctg ggcagccacc
       61 gggaagggca ggatgccaga gtgggcattc tcagggggtc ggggccgggc cgccgccggt
      121 ccctctccgc cgccttgttg cgcggccgcg gctcgcccgc acagcctgtg ggcagaacgg
      181 cgtgctgctt gctcttcggg ctcccggcct cctcgcgccg gtctccccgg gtagttattt
      241 gtcgtatccc gggatgggcg atacgacacc ctacccaccc tactctgcgg aaagttgaac
      301 tcctactcgc cctgagctcc tcctcagccc ttgccggcct tcactcgcct ctcagattct
      361 taacgctata tgactttggc tttcaagatc ctcttcccga gaaacctttg tgcccttggc
      421 agaaaagaac tgtgcctgtt cccagaacag aatcgctggg ctgccataag tcagttcagc
      481 cagtggtcgg aaacaaatct cctcggtggg tgctgccttc tccagagaag aaagcctgtc
      541 ctagctctcc agagaggtca tctaagacca cgtgccaccc accttacttt ctggccaggg
      601 tcgcatgtgg gactctgtac tggcccctgt gcgatggcag agcagcggtt ctgtgtggac
      661 tatgccaagc ggggcacagc tggatgcaag aaatgcaagg agaagattgt aaagggcgtg
      721 tgccgcattg gcaaagtggt gcccaacccc ttctcagagt ctgggggtga tatgaaagag
      781 tggtaccaca ttaagtgcat gtttgagaaa ctggaacggg ctcgggccac cacaaagaaa
      841 attgaagacc tcacagagct ggaaggctgg gaagagctgg aagataacga aaaggaacag
      901 ataagccagc atattgcaga cctgtcttct aaggcagctg ccacacctaa gaagaaagct
      961 gctgtccagg ctaagctgac aaccactggc caggtgacat ctccagtgaa aggtgcttcg
     1021 tttattacca gtaccaatcc tcggaagttc tctggctttt cagcagcaaa acccaacaac
     1081 tctgagcaag ccccctcaag ccctgctcct ggaacgagtc tgtctgcaag taaatgtgac
     1141 cctaagcaca aagattgtct gctacgagag ttccggaagc tgtgcgccat ggtggctgaa
     1201 aatcctagct ataacaccaa gacccagatc atccatgact tcttgcagaa aggctccaca
     1261 ggagatggct ttcacggtga tgtgtacctg acagttaagc tgctgctgcc tggtgtcatc
     1321 aagagtgttt acaacttgaa tgataagcag attgtaaaac tttttagccg aatttttaac
     1381 tgcaacccag atgatatggc ccgggaccta gaacagggtg acgtgtcaga gacgatcaga
     1441 atcttctttg agcagagcaa gtctttcccc ccagctgcca aaagcctcct caccattcag
     1501 gaagtggatg ccttcctcct gcacctctcc aagctcacca aagaggatga gcagcagcag
     1561 gccctgcagg acatagcctc ccggtgtaca gccaatgacc ttaagtgcat tattcggctg
     1621 atcaaacatg atctgaagat gaactcgggt gcaaaacacg tgttagatgc ccttgacccc
     1681 aatgcttatg aagccttcaa agcatcgcgc aacctgcagg atgtggtgga gcgagtcctt
     1741 cacaatgagc aggaggtgga gaaggaccca ggccggcgac gggctctgag ggtccaggcc
     1801 tcactgatga cgcctgtgca gcccatgctg gctgaggcct gcaaatccat tgaatatgca
     1861 atgaagaagt gtcccaatgg catgttctct gagatcaagt acgatggtga gcgagtccag
     1921 gtgcataaga agggggacca cttcagctac ttcagccgca gtctcaagcc tgtcctgcct
     1981 cacaaggtgg cccacttcaa ggactatata cccaaagctt ttcctggggg tcaaagcatg
     2041 atcttggatt ccgaggtact cctgattgac aacaacacag gcaaaccact gccctttggg
     2101 actctgggag tgcacaagaa agctgctttc caggatgcta atgtctgcct gtttgttttt
     2161 gattgtatct actttaatga tgtcagcttg atggacaggc ctctctgtga gcgacggaag
     2221 tttcttcatg acaacatggt tgaaatccgt aaccggatca tgttctcaga aatgaagcaa
     2281 gtcacaaaag cttcagacct ggctgacatg ataaaccggg tgatccgaga gggcttggaa
     2341 gggttggtgc taaaggatgt gaagggtaca tatgagcctg ggaagcggca ctggctgaaa
     2401 gttaagaaag attatttgaa tgagggggcc atggctgata cagctgatct ggtggttctt
     2461 ggggccttct atgggcaagg gagcaaaggc ggaatgatgt ccatcttcct catgggctgc
     2521 tatgaccctg acagccaaaa gtggtgcact gttacaaaat gtgctggagg ccatgatgat
     2581 gccacacttg cccgcctgca gaaagagctg gttatggtga agatcagcaa ggatcctagt
     2641 aagataccca gctggctgaa gatcaacaag atctattatc ctgactttat cgtcccagac
     2701 ccaaagaaag ctgctgtgtg ggagatcaca ggggcagaat tctccagatc tgaggcacac
     2761 actgctgatg gcatctccat ccgatttcct cgatgcaccc gaatccggga cgacaaggac
     2821 tggaaatctg ccactaacct cccccaactc aaggaactgt accagctgtc caaagacaag
     2881 gcagactttg ctgtggtggc tggagatgag gcaagcccca ctactggagg cagcagtggc
     2941 gagaacgagg gcactgctgg gtctgctggg ccctgcaagg gcccccccag taagtcctct
     3001 gccagtgcca agaccaccga acagaagctc aatagcccca gtagcagagg tggcatcaaa
     3061 ccgattccaa agcattcccc catgaaacca ggagagaagc tggctgtgaa gtcttctcca
     3121 gtcaaagtgg gaatgaagag gaaagctact gatgagaccc catgcctgaa aaaggtactg
     3181 ttggatgtct tcactggggt gcggctctac ttgccacctt ctacaccaga cttcaaacgt
     3241 ctcaaacgct actttgtggc attcgacggg gacctggtac aggaatttga catgggctca
     3301 gccacacatg tgctaggtaa cagggaaaag aacactgatg cccagttggt ctcctcagag
     3361 tggatttggg catgtatccg gaaacggagg ctgatagctc cctgctagga ctttggtctt
     3421 cctctttggg ctgtcctctc ctgcccactc taccacatga ggctcagcct gaagggtagg
     3481 ggcctctttg ccaagcagcc tactttctta tatcctctga ttcttcatgg tctcttctgg
     3541 atcaggagtt aactgctgtg gacacaagca gtttaactgg aactttgaga tccgggtgct
     3601 agctttgtct tttttattag ttgtctgtgg tactgataac gaacttagac ccttattcat
     3661 gctatgcaca gcactctgcc actgtcctac agttttataa ataagaaatc ttaagctagg
     3721 catgaagaag tcatggggat cagaagttca aggctaacct gggataagaa tttaaacaag
     3781 aataagtatc cttcccccac tggttccacc tgcagttttc ctccgagact aaggaatgaa
     3841 ggctgggcag agcctacttc ctgtgctcct cgcttccatg tgcatcttaa aagctgtatt
     3901 taaactcttc tcgaatgctt cttgtatgtg gcaagacctt tattatgcct ggtctaaacc
     3961 ctcacgtgaa agaacaaagt ggcatccaat gattagagtt tccaggtcac tgcctggagg
     4021 gagaggctga catctacagg atggcttcct tggagacaga ggctccttga aggtgaattt
     4081 gcctcctcaa aatctgacta ctcttaggag cacaggtcca tagctgggaa aatgtacaca
     4141 ccctgcttac caaggctggc tcctgtatca tgtgtggcag atgcacttga agggtaaaga
     4201 aggatgattg ctgtgtacaa gtgcacacaa gggtattttg caacagtgga agtgtcctaa
     4261 aataggctca tctgattatc aagaccttga aatgtggctg gcatgactgg agaaaaccat
     4321 aaacgtttca agacaacttc aaatggtctc aagttaccag tgacatcaca tatcacctga
     4381 gcatcagttc aagaggagtg ggtagagtca ctgttgctga atcactgggc agctgccact
     4441 aacctcagac accttgttta aggtgtgcag cgcacaccct ggttcagagg ttgtccagga
     4501 ggaggcctag cttaccaact catgttaaaa gttcgcattc aggtctaagc ctggccctcg
     4561 ccaggaactg tgggagactg gtttccttgt ttatgctgtg ccacctcttg agaatcctct
     4621 ctctgttctt accaactcag caggcttaca tatccacctc ctgtgagcta ggttactagg
     4681 atggaacttg tgagcctcta agatacagac ccaccacccc atgtaactcc taaagtaaga
     4741 tcccatcagg cggttgctta cttgtctggc tctatctttc acctgctctt taagacatgg
     4801 atgtagatgc tctgatgagg gcagagtagg caacattcag tctggtaagt aaagcattta
     4861 actctagtct caagcagaag ctgctttgcg ctttgaacac ttggaccaag cagcttggtc
     4921 cagcatcaga ctattcttcc tcatcacaca gggccaaaag ttcagccctt tgtcccttta
     4981 atgccttact atatatttac tccaactcac tgtcagatct ttgcaaagag gttaaactgt
     5041 atgaagacct ttgtatcttt tcccgtcttt ccacacaacc ctaaggacca tcacctgaaa
     5101 cctcccacac ggctggaaac gtgccctctg tggcctttgt ttacaatagt gccagttgat
     5161 gacaggcatt gttcattgct gggtgctcaa taggtccagc catcagcaag agacagggta
     5221 gaatcctttc atccagttct cccaggggtg atacactccc ctcccctcag gcacctcttc
     5281 aggtaggtgg gtacaggcca gtgcttactc tgtagtctgc tctttaggct gggccaacct
     5341 ttgagacaca cttgtctgta ttcaaaagct acctactgtc agtctttcag ggtaacttgt
     5401 tcctttccac ttagctcttg aaggcccctc tacagtggga ccgtaactaa cttcacatgg
     5461 tgcttcccgt ctgctcttga gaatcttaca cttagctcca cttccaactg tcccacagtt
     5521 tctccaatgc attcttgtga tcttgggtga gtcacaccac agagcaaggg taccttgatc
     5581 aagaccaggt ggccaagcac ctccagaagc gggatgcagt ctctgaagag caatgttctg
     5641 catgtggcct gatgtctgcc tgcatttaca agttctctgg cattatcccc cacacaccag
     5701 atgggacagg catctgtatt taaactttat tacaaaatta taaagcagag ctctgtaaca
     5761 aaaaaataca catttgggta tgcttttaac acccaggtga gaaaatctta atgtaagcca
     5821 cttggagcta cacatttaca tccgagagat ctcaacaaat ttgtacaaca tagactatta
     5881 attcctttac agcttattag tttagaaccg gctatttcca acctatgaaa cagcatcttt
     5941 gaaaactaag tctcaactga aatcttggtg caatcagcaa aatccacaga ataattggaa
     6001 agattaggtg tttcaacatt aagaccaagg atccgtgaag aacaaaaagg gggctcaggt
     6061 atttaaccaa aaaaaaaaaa aaaaaaaatt aaggttttct acctgagatg ttaactggaa
     6121 tgggttccaa ctttcatttg gtttaacaat aaagttgttt gacatgtt
//
