LOCUS       NM_024471               2884 bp    mRNA    linear   ROD 31-MAY-2024
DEFINITION  Mus musculus lipoic acid synthetase (Lias), transcript variant 1,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_024471
VERSION     NM_024471.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Xu,G., Zhao,Y., Tao,Y., Xiong,C., Lv,M., Gao,Q., Zhang,F., An,Z.
            and Wu,W.
  TITLE     Lias overexpression alleviates pulmonary injury induced by fine
            particulate matter in mice
  JOURNAL   Environ Geochem Health 45 (8), 6585-6603 (2023)
   PUBMED   37341891
  REMARK    GeneRIF: Lias overexpression alleviates pulmonary injury induced by
            fine particulate matter in mice.
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Xu,G., Yan,T., Peng,Q., Li,H., Wu,W., Yi,X. and Zhao,Y.
  TITLE     Overexpression of the Lias gene attenuates hepatic steatosis in
            Leprdb/db mice
  JOURNAL   J Endocrinol 248 (2), 119-131 (2021)
   PUBMED   33263565
  REMARK    GeneRIF: Overexpression of the Lias gene attenuates hepatic
            steatosis in Leprdb/db mice.
REFERENCE   3  (bases 1 to 2884)
  AUTHORS   Xu,L., Hiller,S., Simington,S., Nickeleit,V., Maeda,N., James,L.R.
            and Yi,X.
  TITLE     Influence of Different Levels of Lipoic Acid Synthase Gene
            Expression on Diabetic Nephropathy
  JOURNAL   PLoS One 11 (10), e0163208 (2016)
   PUBMED   27706190
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2884)
  AUTHORS   Yi,X., Xu,L., Hiller,S., Kim,H.S. and Maeda,N.
  TITLE     Reduced alpha-lipoic acid synthase gene expression exacerbates
            atherosclerosis in diabetic apolipoprotein E-deficient mice
  JOURNAL   Atherosclerosis 223 (1), 137-143 (2012)
   PUBMED   22658261
  REMARK    GeneRIF: Decreased endogenous lipoic acid gene expression plays a
            role in development of diabetic atherosclerosis in ApoE deficient
            mice.
REFERENCE   5  (bases 1 to 2884)
  AUTHORS   Yi,X., Xu,L., Hiller,S., Kim,H.S., Nickeleit,V., James,L.R. and
            Maeda,N.
  TITLE     Reduced expression of lipoic acid synthase accelerates diabetic
            nephropathy
  JOURNAL   J Am Soc Nephrol 23 (1), 103-111 (2012)
   PUBMED   22021711
  REMARK    GeneRIF: deficiency increases oxidative stress and accelerates the
            development of diabetic nephropathy
REFERENCE   6  (bases 1 to 2884)
  AUTHORS   Yi,X., Kim,K., Yuan,W., Xu,L., Kim,H.S., Homeister,J.W., Key,N.S.
            and Maeda,N.
  TITLE     Mice with heterozygous deficiency of lipoic acid synthase have an
            increased sensitivity to lipopolysaccharide-induced tissue injury
  JOURNAL   J Leukoc Biol 85 (1), 146-153 (2009)
   PUBMED   18845616
  REMARK    GeneRIF: Enhanced neutrophil sequestration in the livers of Lias+/-
            mice was associated with increased hepatocyte injury and increased
            gene expression of growth-related oncogene, E-selectin, and VCAM-1
            in the liver and/or lung.
REFERENCE   7  (bases 1 to 2884)
  AUTHORS   Yi,X. and Maeda,N.
  TITLE     Endogenous production of lipoic acid is essential for mouse
            development
  JOURNAL   Mol Cell Biol 25 (18), 8387-8392 (2005)
   PUBMED   16135825
  REMARK    GeneRIF: Development of homozygous embryos lacking Lias is
            retarded.
REFERENCE   8  (bases 1 to 2884)
  AUTHORS   Garcia-Garcia,M.J., Eggenschwiler,J.T., Caspary,T., Alcorn,H.L.,
            Wyler,M.R., Huangfu,D., Rakeman,A.S., Lee,J.D., Feinberg,E.H.,
            Timmer,J.R. and Anderson,K.V.
  TITLE     Analysis of mouse embryonic patterning and morphogenesis by forward
            genetics
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 5913-5919 (2005)
   PUBMED   15755804
REFERENCE   9  (bases 1 to 2884)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   10 (bases 1 to 2884)
  AUTHORS   Morikawa,T., Yasuno,R. and Wada,H.
  TITLE     Do mammalian cells synthesize lipoic acid? Identification of a
            mouse cDNA encoding a lipoic acid synthase located in mitochondria
  JOURNAL   FEBS Lett 498 (1), 16-21 (2001)
   PUBMED   11389890
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077192.1 and AK048744.1.
            
            On Jul 16, 2015 this sequence version replaced NM_024471.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048744.1, AK077192.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1666              AK077192.1         2-1667
            1667-2884           AK048744.1         1611-2828
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 33.66 cM"
     gene            1..2884
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /note="lipoic acid synthetase"
                     /db_xref="GeneID:79464"
                     /db_xref="MGI:MGI:1934604"
     exon            1..177
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..1254
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /EC_number="2.8.1.8"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; lipoyl synthase, mitochondrial; lipoate
                     synthase; lipoic acid synthase"
                     /codon_start=1
                     /product="lipoyl synthase, mitochondrial isoform 1
                     precursor"
                     /protein_id="NP_077791.1"
                     /db_xref="CCDS:CCDS19307.1"
                     /db_xref="GeneID:79464"
                     /db_xref="MGI:MGI:1934604"
                     /translation="MALRCWDTARSLGSRIFGRYAFTVRALSSLPDKKKEFLHNGPDL
                     QDFVSGDLADKSTWDEYKGNLKRQKGERLRLPPWLKTKIPMGKNYNKLKNTLRNLSLH
                     TVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGCRFCSVKTARNPPPLDANEPD
                     NTAKAIAEWGLDYVVLTSVDRDDVADGGAEHIAKTVSCLKERNPKILVECLTPDFRGD
                     LRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLRHAKEVQPDVVSKTS
                     IMLGLGETDEQVYATLKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEKVG
                     NELGFLYTASGPLVRSSYKAGEFFLKNLVARRKTKASKV"
     exon            178..347
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            348..441
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            442..522
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            523..679
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            680..737
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            738..866
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1012
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1083
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1195
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..2884
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1616..1621
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /note="hexamer: TATAAA"
     regulatory      1621..1626
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /note="hexamer: ATTACA"
     polyA_site      1637
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
     polyA_site      1641
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /note="major polyA site"
ORIGIN      
        1 gagctagtcg agtcgcgccg gtcggcgacg acgtatcgcg tcatgactca agcgacgagt
       61 tgttggacgc gtccggtgcc gggggtgctg ttccctcgat cccttgacac tcctgagacc
      121 taaaagaggg ggatggctct acgctgctgg gatacagctc gcagcctggg gtcccggata
      181 tttggaagat atgcgttcac ggttagagca ttaagttctt tgccagataa gaaaaaggaa
      241 tttttgcata atggaccaga ccttcaagat tttgtatctg gtgatcttgc agacaagagt
      301 acatgggatg aatataaagg aaacttaaag cgccagaaag gagaaaggct aagactacct
      361 ccatggctaa agacaaagat acccatgggt aaaaactaca ataaactgaa aaatacattg
      421 cggaatttaa gtctccacac agtgtgtgag gaagcccggt gccccaacat tggagagtgt
      481 tggggaggtg gggaatatgc cacagccaca gccacgatca tgttgatggg ggacacatgc
      541 acaagaggtt gcagattttg ttccgttaag accgcaagaa atccccctcc attggatgcc
      601 aatgagcccg acaatacggc caaagcaatt gcagagtggg gtctggatta tgttgtcctg
      661 acgtcggtgg atcgagatga tgtggccgac gggggagctg agcacatcgc caagaccgtg
      721 tcatgcttga aggaaaggaa tccaaaaatc ctcgtggaat gcctcacccc agacttccga
      781 ggtgatctga gagcagtgga gaaggtggct ctgtctggat tagatgtgta tgcacacaat
      841 gtggagactg tccccgagtt acagaggaaa gttcgtgatc cccgggccaa ttttgaccag
      901 tctcttcgtg tactgagaca tgccaaggag gtccagcctg atgttgtttc caaaacatcg
      961 ataatgctgg gccttggtga gacagacgag caagtctatg ccacactgaa agctctccgt
     1021 gcggccgacg tggactgttt aactctaggg cagtacatgc agccaacgaa gcgccacctt
     1081 aaggttgaag aatatgttac tcctgagaag ttcaaatact gggaaaaagt agggaatgaa
     1141 cttggatttc tctatacggc aagtggtcct ttggtgcgat cttcatataa agcaggtgaa
     1201 tttttcctga aaaatctagt ggctagaaga aaaacaaaag cttctaaagt gtagccaagc
     1261 ccttcaagat cacaggagtg ttgcgatttt gcttccagtt ggtgtgagga tggtgtcacc
     1321 ctgtcaggat tcctgtgagt gcaaggcagc accgttctga agggtctgat ggctgatcga
     1381 agcggaatgg tgtatacctg taattccacc caggaaaaaa aaaaggaaaa gaaaaagaga
     1441 aaagaagaaa gaaaagaagc ctataaccct gtaaacgaca cacatttagt gataattcat
     1501 ggacttcttg ctggcctttg tcctcatcct tgtcagtgat gacttgatca cagaacagct
     1561 tggaacggct tgctgttgcc attgtaattc aagtctatga acatttagaa gatcttataa
     1621 attacagtaa cccatgtgaa atttcttttc ttaaaaagat ttatttttat ttgcgtgtgt
     1681 gtatgtgtgt gtgtgtgtgt gtgttagtgt gttagaggaa cacatgcgca cacacacata
     1741 tggactccgt gacacataat aatctttgta agtcagcccc agtgtggagt tgggaaggct
     1801 cacgagctcc caataatgtc agtagctgag gaggggaggc ggtcttcttt aagggtgtgg
     1861 cctttgtgtg acccatgctc tagagcagtg gctctcagcc tttctcacgc tgaggccctt
     1921 taatacggtt cctaaagtat ggtgacccca gccatcaaat tagctttgtt gctacttcat
     1981 taccgtgatt ttgctactgt tcatgagttg taagtgtgtg atacccaggg tattgtgacc
     2041 cttaccctcc cgctagggtc gagcctttgt ggtccttatg gacacttcct cttgtagtca
     2101 ttacataccc atgcactctg ctgtccacaa atgtcgtaaa gcctctgggc cactgctggc
     2161 tccttcctac tcttgtttta ttgaattaca cagcccctac tggtacctct tgctactctt
     2221 aattatagtg ggttccttac aaaaagaaca tacctgtgtg cacaggataa gacatgatgg
     2281 tacacacttt gaacctcagc agttgtgtgg aagattgcag tgagttgaag gccagcctgg
     2341 gtcaaagccc tttattatct gtccttccaa actggatgtg gactttttgt tgtttgttga
     2401 gacagggtct catgcagccc aggctactca gactggctgt gtggtagagg atgacccctg
     2461 atgtcctggc tctgtcccta agtgctgtga tcacaggcct gcaacactag ctggcaagga
     2521 gtacactctc acaccagtac ttggaggcaa tggagaagat cacagtattc caggccagtc
     2581 tagactgtgc agcaaagtca cccaaagtca ccttccttac ctgcctcaag caatcttctg
     2641 ttcctcatgt tcctcctgaa ctccagacca aacccagcct ttattttcct gtctgttgtg
     2701 tgggctatga gacagggtct cacagtgtag caaaggttat cacagaactc agttctgtag
     2761 tcagggctgt cctggaactc ctggcatctg cctgctctgc ctcacattac agggagaaag
     2821 ccactccact ctgcctcctg cctctttttg ttgttgctaa attttaaaat aagatctact
     2881 tttt
//
