LOCUS       NM_001310612            2769 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus lipoic acid synthetase (Lias), transcript variant 2,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001310612 XM_006504194
VERSION     NM_001310612.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Xu,G., Zhao,Y., Tao,Y., Xiong,C., Lv,M., Gao,Q., Zhang,F., An,Z.
            and Wu,W.
  TITLE     Lias overexpression alleviates pulmonary injury induced by fine
            particulate matter in mice
  JOURNAL   Environ Geochem Health 45 (8), 6585-6603 (2023)
   PUBMED   37341891
  REMARK    GeneRIF: Lias overexpression alleviates pulmonary injury induced by
            fine particulate matter in mice.
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Xu,G., Yan,T., Peng,Q., Li,H., Wu,W., Yi,X. and Zhao,Y.
  TITLE     Overexpression of the Lias gene attenuates hepatic steatosis in
            Leprdb/db mice
  JOURNAL   J Endocrinol 248 (2), 119-131 (2021)
   PUBMED   33263565
  REMARK    GeneRIF: Overexpression of the Lias gene attenuates hepatic
            steatosis in Leprdb/db mice.
REFERENCE   3  (bases 1 to 2769)
  AUTHORS   Xu,L., Hiller,S., Simington,S., Nickeleit,V., Maeda,N., James,L.R.
            and Yi,X.
  TITLE     Influence of Different Levels of Lipoic Acid Synthase Gene
            Expression on Diabetic Nephropathy
  JOURNAL   PLoS One 11 (10), e0163208 (2016)
   PUBMED   27706190
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2769)
  AUTHORS   Yi,X., Xu,L., Hiller,S., Kim,H.S. and Maeda,N.
  TITLE     Reduced alpha-lipoic acid synthase gene expression exacerbates
            atherosclerosis in diabetic apolipoprotein E-deficient mice
  JOURNAL   Atherosclerosis 223 (1), 137-143 (2012)
   PUBMED   22658261
  REMARK    GeneRIF: Decreased endogenous lipoic acid gene expression plays a
            role in development of diabetic atherosclerosis in ApoE deficient
            mice.
REFERENCE   5  (bases 1 to 2769)
  AUTHORS   Yi,X., Xu,L., Hiller,S., Kim,H.S., Nickeleit,V., James,L.R. and
            Maeda,N.
  TITLE     Reduced expression of lipoic acid synthase accelerates diabetic
            nephropathy
  JOURNAL   J Am Soc Nephrol 23 (1), 103-111 (2012)
   PUBMED   22021711
  REMARK    GeneRIF: deficiency increases oxidative stress and accelerates the
            development of diabetic nephropathy
REFERENCE   6  (bases 1 to 2769)
  AUTHORS   Yi,X., Kim,K., Yuan,W., Xu,L., Kim,H.S., Homeister,J.W., Key,N.S.
            and Maeda,N.
  TITLE     Mice with heterozygous deficiency of lipoic acid synthase have an
            increased sensitivity to lipopolysaccharide-induced tissue injury
  JOURNAL   J Leukoc Biol 85 (1), 146-153 (2009)
   PUBMED   18845616
  REMARK    GeneRIF: Enhanced neutrophil sequestration in the livers of Lias+/-
            mice was associated with increased hepatocyte injury and increased
            gene expression of growth-related oncogene, E-selectin, and VCAM-1
            in the liver and/or lung.
REFERENCE   7  (bases 1 to 2769)
  AUTHORS   Yi,X. and Maeda,N.
  TITLE     Endogenous production of lipoic acid is essential for mouse
            development
  JOURNAL   Mol Cell Biol 25 (18), 8387-8392 (2005)
   PUBMED   16135825
  REMARK    GeneRIF: Development of homozygous embryos lacking Lias is
            retarded.
REFERENCE   8  (bases 1 to 2769)
  AUTHORS   Garcia-Garcia,M.J., Eggenschwiler,J.T., Caspary,T., Alcorn,H.L.,
            Wyler,M.R., Huangfu,D., Rakeman,A.S., Lee,J.D., Feinberg,E.H.,
            Timmer,J.R. and Anderson,K.V.
  TITLE     Analysis of mouse embryonic patterning and morphogenesis by forward
            genetics
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 5913-5919 (2005)
   PUBMED   15755804
REFERENCE   9  (bases 1 to 2769)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   10 (bases 1 to 2769)
  AUTHORS   Morikawa,T., Yasuno,R. and Wada,H.
  TITLE     Do mammalian cells synthesize lipoic acid? Identification of a
            mouse cDNA encoding a lipoic acid synthase located in mitochondria
  JOURNAL   FEBS Lett 498 (1), 16-21 (2001)
   PUBMED   11389890
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077192.1, AK045167.1 and AK048744.1.
            
            On Jul 16, 2015 this sequence version replaced XM_006504194.2.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' region and initiates translation at a downstream start
            codon compared to variant 1. The encoded isoform (2) has a shorter
            N-terminus than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045167.1, SRR7652917.435270.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               AK077192.1         2-102
            102-1296            AK045167.1         2-1196
            1297-2769           AK048744.1         1356-2828
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 33.66 cM"
     gene            1..2769
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /note="lipoic acid synthetase"
                     /db_xref="GeneID:79464"
                     /db_xref="MGI:MGI:1934604"
     exon            1..177
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            178..232
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            233..326
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..1139
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /EC_number="2.8.1.8"
                     /note="isoform 2 is encoded by transcript variant 2;
                     lipoyl synthase, mitochondrial; lipoate synthase; lipoic
                     acid synthase"
                     /codon_start=1
                     /product="lipoyl synthase, mitochondrial isoform 2"
                     /protein_id="NP_001297541.1"
                     /db_xref="CCDS:CCDS80288.1"
                     /db_xref="GeneID:79464"
                     /db_xref="MGI:MGI:1934604"
                     /translation="MGKNYNKLKNTLRNLSLHTVCEEARCPNIGECWGGGEYATATAT
                     IMLMGDTCTRGCRFCSVKTARNPPPLDANEPDNTAKAIAEWGLDYVVLTSVDRDDVAD
                     GGAEHIAKTVSCLKERNPKILVECLTPDFRGDLRAVEKVALSGLDVYAHNVETVPELQ
                     RKVRDPRANFDQSLRVLRHAKEVQPDVVSKTSIMLGLGETDEQVYATLKALRAADVDC
                     LTLGQYMQPTKRHLKVEEYVTPEKFKYWEKVGNELGFLYTASGPLVRSSYKAGEFFLK
                     NLVARRKTKASKV"
     exon            327..407
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            408..564
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            565..622
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            623..751
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            752..897
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            898..968
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1080
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..2769
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1501..1506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /note="hexamer: TATAAA"
     regulatory      1506..1511
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /note="hexamer: ATTACA"
     polyA_site      1522
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
     polyA_site      1526
                     /gene="Lias"
                     /gene_synonym="2900022L22Rik; 4933425M12Rik; 7a5ex;
                     lip-syn; LS"
                     /note="major polyA site"
ORIGIN      
        1 gagctagtcg agtcgcgccg gtcggcgacg acgtatcgcg tcatgactca agcgacgagt
       61 tgttggacgc gtccggtgcc gggggtgctg ttccctcgat cccttgacac tcctgagacc
      121 taaaagaggg ggatggctct acgctgctgg gatacagctc gcagcctggg gtcccggaca
      181 agagtacatg ggatgaatat aaaggaaact taaagcgcca gaaaggagaa aggctaagac
      241 tacctccatg gctaaagaca aagataccca tgggtaaaaa ctacaataaa ctgaaaaata
      301 cattgcggaa tttaagtctc cacacagtgt gtgaggaagc ccggtgcccc aacattggag
      361 agtgttgggg aggtggggaa tatgccacag ccacagccac gatcatgttg atgggggaca
      421 catgcacaag aggttgcaga ttttgttccg ttaagaccgc aagaaatccc cctccattgg
      481 atgccaatga gcccgacaat acggccaaag caattgcaga gtggggtctg gattatgttg
      541 tcctgacgtc ggtggatcga gatgatgtgg ccgacggggg agctgagcac atcgccaaga
      601 ccgtgtcatg cttgaaggaa aggaatccaa aaatcctcgt ggaatgcctc accccagact
      661 tccgaggtga tctgagagca gtggagaagg tggctctgtc tggattagat gtgtatgcac
      721 acaatgtgga gactgtcccc gagttacaga ggaaagttcg tgatccccgg gccaattttg
      781 accagtctct tcgtgtactg agacatgcca aggaggtcca gcctgatgtt gtttccaaaa
      841 catcgataat gctgggcctt ggtgagacag acgagcaagt ctatgccaca ctgaaagctc
      901 tccgtgcggc cgacgtggac tgtttaactc tagggcagta catgcagcca acgaagcgcc
      961 accttaaggt tgaagaatat gttactcctg agaagttcaa atactgggaa aaagtaggga
     1021 atgaacttgg atttctctat acggcaagtg gtcctttggt gcgatcttca tataaagcag
     1081 gtgaattttt cctgaaaaat ctagtggcta gaagaaaaac aaaagcttct aaagtgtagc
     1141 caagcccttc aagatcacag gagtgttgcg attttgcttc cagttggtgt gaggatggtg
     1201 tcaccctgtc aggattcctg tgagtgcaag gcagcaccgt tctgaagggt ctgatggctg
     1261 atcgaagcgg aatggtgtat acctgtaatt ccacccagga aaaaaaaaag gaaaagaaaa
     1321 agagaaaaga agaaagaaaa gaagcctata accctgtaaa cgacacacat ttagtgataa
     1381 ttcatggact tcttgctggc ctttgtcctc atccttgtca gtgatgactt gatcacagaa
     1441 cagcttggaa cggcttgctg ttgccattgt aattcaagtc tatgaacatt tagaagatct
     1501 tataaattac agtaacccat gtgaaatttc ttttcttaaa aagatttatt tttatttgcg
     1561 tgtgtgtatg tgtgtgtgtg tgtgtgtgtt agtgtgttag aggaacacat gcgcacacac
     1621 acatatggac tccgtgacac ataataatct ttgtaagtca gccccagtgt ggagttggga
     1681 aggctcacga gctcccaata atgtcagtag ctgaggaggg gaggcggtct tctttaaggg
     1741 tgtggccttt gtgtgaccca tgctctagag cagtggctct cagcctttct cacgctgagg
     1801 ccctttaata cggttcctaa agtatggtga ccccagccat caaattagct ttgttgctac
     1861 ttcattaccg tgattttgct actgttcatg agttgtaagt gtgtgatacc cagggtattg
     1921 tgacccttac cctcccgcta gggtcgagcc tttgtggtcc ttatggacac ttcctcttgt
     1981 agtcattaca tacccatgca ctctgctgtc cacaaatgtc gtaaagcctc tgggccactg
     2041 ctggctcctt cctactcttg ttttattgaa ttacacagcc cctactggta cctcttgcta
     2101 ctcttaatta tagtgggttc cttacaaaaa gaacatacct gtgtgcacag gataagacat
     2161 gatggtacac actttgaacc tcagcagttg tgtggaagat tgcagtgagt tgaaggccag
     2221 cctgggtcaa agccctttat tatctgtcct tccaaactgg atgtggactt tttgttgttt
     2281 gttgagacag ggtctcatgc agcccaggct actcagactg gctgtgtggt agaggatgac
     2341 ccctgatgtc ctggctctgt ccctaagtgc tgtgatcaca ggcctgcaac actagctggc
     2401 aaggagtaca ctctcacacc agtacttgga ggcaatggag aagatcacag tattccaggc
     2461 cagtctagac tgtgcagcaa agtcacccaa agtcaccttc cttacctgcc tcaagcaatc
     2521 ttctgttcct catgttcctc ctgaactcca gaccaaaccc agcctttatt ttcctgtctg
     2581 ttgtgtgggc tatgagacag ggtctcacag tgtagcaaag gttatcacag aactcagttc
     2641 tgtagtcagg gctgtcctgg aactcctggc atctgcctgc tctgcctcac attacaggga
     2701 gaaagccact ccactctgcc tcctgcctct ttttgttgtt gctaaatttt aaaataagat
     2761 ctacttttt
//
