LOCUS       NP_034325                907 aa            linear   ROD 03-JUN-2025
DEFINITION  leucine-rich repeat-containing G-protein coupled receptor 5
            precursor [Mus musculus].
ACCESSION   NP_034325
VERSION     NP_034325.2
DBSOURCE    REFSEQ: accession NM_010195.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 907)
  AUTHORS   Danek,V., Tureckova,J., Huebner,K., Erlenbach-Wuensch,K.,
            Baranova,P., Dobes,J., Balounova,J., Simova,M., Novosadova,V.,
            Madureira Trufen,C.E., Prochazkova,M., Talacko,P., Harant,K.,
            Barinka,C., Beck,I.M., Schneider-Stock,R., Sedlacek,R. and
            Prochazka,J.
  TITLE     CUL4A exhibits tumor-suppressing role via regulation of
            HUWE1-mediated SMAD3 intracellular shuttling
  JOURNAL   Cancer Lett 621, 217663 (2025)
   PUBMED   40120800
REFERENCE   2  (residues 1 to 907)
  AUTHORS   Tuwatnawanit,T., Wessman,W., Belisova,D., Sumbalova Koledova,Z.,
            Tucker,A.S. and Anthwal,N.
  TITLE     FSP1/S100A4-Expressing Stem/Progenitor Cells Are Essential for
            Temporomandibular Joint Growth and Homeostasis
  JOURNAL   J Dent Res 104 (5), 551-560 (2025)
   PUBMED   39953712
REFERENCE   3  (residues 1 to 907)
  AUTHORS   Peters,F., Hofs,W., Lee,H., Brodesser,S., Kruse,K., Drexler,H.C.A.,
            Hu,J., Raker,V.K., Lukas,D., von Stebut,E., Kronke,M., Niessen,C.M.
            and Wickstrom,S.A.
  TITLE     Sphingolipid metabolism orchestrates establishment of the hair
            follicle stem cell compartment
  JOURNAL   J Cell Biol 224 (4) (2025)
   PUBMED   39879198
REFERENCE   4  (residues 1 to 907)
  AUTHORS   Nakajima-Koyama,M., Kabata,M., Lee,J., Sogabe,Y., Sakurai,S.,
            Hirota,A., Kimura,M., Nakamura,T., Imoto,Y., Kometani,K.,
            Hamazaki,Y., Hiraoka,Y., Saitou,M., Nishida,E. and Yamamoto,T.
  TITLE     The balance between IFN-gamma and ERK/MAPK signaling activities
            ensures lifelong maintenance of intestinal stem cells
  JOURNAL   Cell Rep 44 (3), 115286 (2025)
   PUBMED   39952238
REFERENCE   5  (residues 1 to 907)
  AUTHORS   Seo,D.W., Hong,K.T., Lee,J.H., Lee,J.S. and Jeong,Y.T.
  TITLE     Dual independent mechanisms underlying gut epithelial remodeling
            upon sugar substitute consumption
  JOURNAL   FASEB J 39 (3), e70374 (2025)
   PUBMED   39902882
REFERENCE   6  (residues 1 to 907)
  AUTHORS   Alexandrovich,A., Qureishi,A., Coudert,A.E., Zhang,L.,
            Grigoriadis,A.E., Shah,A.M., Brewer,A.C. and Pizzey,J.A.
  TITLE     A role for GATA-6 in vertebrate chondrogenesis
  JOURNAL   Dev Biol 314 (2), 457-470 (2008)
   PUBMED   18191120
REFERENCE   7  (residues 1 to 907)
  AUTHORS   Barker,N., van Es,J.H., Kuipers,J., Kujala,P., van den Born,M.,
            Cozijnsen,M., Haegebarth,A., Korving,J., Begthel,H., Peters,P.J.
            and Clevers,H.
  TITLE     Identification of stem cells in small intestine and colon by marker
            gene Lgr5
  JOURNAL   Nature 449 (7165), 1003-1007 (2007)
   PUBMED   17934449
  REMARK    GeneRIF: using two knock-in alleles, exclusive expression of Lgr5
            in cycling columnar cells at the crypt base of the intesine is
            revealed
REFERENCE   8  (residues 1 to 907)
  AUTHORS   Nef,S., Schaad,O., Stallings,N.R., Cederroth,C.R., Pitetti,J.L.,
            Schaer,G., Malki,S., Dubois-Dauphin,M., Boizet-Bonhoure,B.,
            Descombes,P., Parker,K.L. and Vassalli,J.D.
  TITLE     Gene expression during sex determination reveals a robust female
            genetic program at the onset of ovarian development
  JOURNAL   Dev Biol 287 (2), 361-377 (2005)
   PUBMED   16214126
REFERENCE   9  (residues 1 to 907)
  AUTHORS   Morita,H., Mazerbourg,S., Bouley,D.M., Luo,C.W., Kawamura,K.,
            Kuwabara,Y., Baribault,H., Tian,H. and Hsueh,A.J.
  TITLE     Neonatal lethality of LGR5 null mice is associated with
            ankyloglossia and gastrointestinal distension
  JOURNAL   Mol Cell Biol 24 (22), 9736-9743 (2004)
   PUBMED   15509778
  REMARK    GeneRIF: physiological role of the LGR5 signaling system during
            embryonic development
REFERENCE   10 (residues 1 to 907)
  AUTHORS   Hermey,G., Methner,A., Schaller,H.C. and Hermans-Borgmeyer,I.
  TITLE     Identification of a novel seven-transmembrane receptor with
            homology to glycoprotein receptors and its expression in the adult
            and developing mouse
  JOURNAL   Biochem Biophys Res Commun 254 (1), 273-279 (1999)
   PUBMED   9920770
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK132387.1 and BM935530.1.
            
            On Jul 22, 2006 this sequence version replaced NP_034325.1.
            
            Summary: The protein encoded by this gene is a leucine-rich
            repeat-containing receptor (LGR) and member of the G
            protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The
            encoded protein is a receptor for R-spondins and is involved in the
            canonical Wnt signaling pathway. This protein plays a role in the
            formation and maintenance of adult intestinal stem cells during
            postembryonic development. [provided by RefSeq, Sep 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132387.1, AF110818.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 63.51 cM"
     Protein         1..907
                     /product="leucine-rich repeat-containing G-protein coupled
                     receptor 5 precursor"
                     /note="G protein-coupled receptor 49; follicle expressed
                     hormone; orphan G-protein coupled receptor FEX"
                     /calculated_mol_wt=97442
     sig_peptide     1..21
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2243
     Region          33..65
                     /region_name="LRRNT"
                     /note="Leucine rich repeat N-terminal domain; pfam01462"
                     /db_xref="CDD:396168"
     Region          68..91
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          92..468
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          92..115
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          116..139
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          140..163
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          164..187
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          188..211
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          212..235
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          236..258
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          259..282
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          283..304
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          354..375
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          376..389
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          400..423
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          445..467
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          558..831
                     /region_name="7tmA_LGR5"
                     /note="leucine-rich repeats-containing G protein-coupled
                     receptor 5, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd15363"
                     /db_xref="CDD:320485"
     Region          559..585
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320485"
     Region          592..617
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320485"
     Site            order(613,616..617,637..642,644..645,648,693,695..699,721,
                     724..726,728..730,732..733,779,782..783,785..786,789,
                     798..799,801..803,806,809..810)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320485"
     Region          637..667
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320485"
     Region          679..701
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320485"
     Region          721..750
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320485"
     Region          759..789
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320485"
     Region          799..824
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320485"
     CDS             1..907
                     /gene="Lgr5"
                     /gene_synonym="FEX; Gpr49"
                     /coded_by="NM_010195.2:288..3011"
                     /db_xref="CCDS:CCDS24180.1"
                     /db_xref="GeneID:14160"
                     /db_xref="MGI:MGI:1341817"
ORIGIN      
        1 mdtscvhmll sllallqlva agsspgpdai prgcpshchc eldgrmllrv dcsdlglsel
       61 psnlsvftsy ldlsmnnisq lpasllhrlc fleelrlagn althipkgaf tglhslkvlm
      121 lqnnqlrqvp eealqnlrsl qslrldanhi syvppscfsg lhslrhlwld dnaltdvpvq
      181 afrslsalqa mtlalnkihh iadyafgnls slvvlhlhnn rihslgkkcf dglhsletld
      241 lnynnldefp taiktlsnlk elgfhsnnir siperafvgn pslitihfyd npiqfvgvsa
      301 fqhlpelrtl tlngashite fphltgtatl esltltgaki sslpqavcdq lpnlqvldls
      361 ynlledlpsl sgcqklqkid lrhneiyeik gstfqqlfnl rslnlawnki aiihpnafst
      421 lpslikldls snllssfpvt glhglthlkl tgnralqsli psanfpelki iempsayqcc
      481 afggcenvyk isnqwnkddg nsvddlhkkd aglfqvqder dledflldfe edlkalhsvq
      541 cspspgpfkp cehlfgswli rigvwttavl alscnalval tvfrtplyis siklligvia
      601 vvdilmgvss avlaavdaft fgrfaqhgaw wedgigcqiv gflsifases siflltlaal
      661 ergfsvkcss kfevkaplfs lraivllcvl laltiatipl lggskynasp lclplpfgep
      721 sttgymvalv llnslcflim tiaytklycs lekgelenlw dcsmvkhial llfancilyc
      781 pvaflsfssl lnltfispdv ikfillvivp lpsclnplly ivfnphfked mgslgkhtrf
      841 wmrskhasll sinsddvekr scestqalvs fthasiaydl pstsgaspay pmteschlss
      901 vafvpcl
//
