LOCUS       NP_766259                951 aa            linear   ROD 28-AUG-2024
DEFINITION  leucine-rich repeat-containing G-protein coupled receptor 4
            precursor [Mus musculus].
ACCESSION   NP_766259 XP_355385 XP_922218
VERSION     NP_766259.2
DBSOURCE    REFSEQ: accession NM_172671.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 951)
  AUTHORS   Kubrak,O., Jorgensen,A.F., Koyama,T., Lassen,M., Nagy,S., Hald,J.,
            Mazzoni,G., Madsen,D., Hansen,J.B., Larsen,M.R., Texada,M.J.,
            Hansen,J.L., Halberg,K.V. and Rewitz,K.
  TITLE     LGR signaling mediates muscle-adipose tissue crosstalk and protects
            against diet-induced insulin resistance
  JOURNAL   Nat Commun 15 (1), 6126 (2024)
   PUBMED   39033139
  REMARK    GeneRIF: LGR signaling mediates muscle-adipose tissue crosstalk and
            protects against diet-induced insulin resistance.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 951)
  AUTHORS   Zhang,H., Liu,D., Xu,Q.F., Wei,J., Zhao,Y., Xu,D.F., Wang,Y.,
            Liu,Y.J., Zhu,X.Y. and Jiang,L.
  TITLE     Endothelial RSPO3 mediates pulmonary endothelial regeneration by
            LGR4-dependent activation of beta-catenin and ILK signaling
            pathways after inflammatory vascular injury
  JOURNAL   Int J Biol Macromol 269 (Pt 2), 131805 (2024)
   PUBMED   38677673
  REMARK    GeneRIF: Endothelial RSPO3 mediates pulmonary endothelial
            regeneration by LGR4-dependent activation of beta-catenin and ILK
            signaling pathways after inflammatory vascular injury.
REFERENCE   3  (residues 1 to 951)
  AUTHORS   Liang,Y., Luo,C., Sun,L., Feng,T., Yin,W., Zhang,Y.,
            Mulholland,M.W., Zhang,W. and Yin,Y.
  TITLE     Reduction of specific enterocytes from loss of intestinal LGR4
            improves lipid metabolism in mice
  JOURNAL   Nat Commun 15 (1), 4393 (2024)
   PUBMED   38782937
  REMARK    GeneRIF: Reduction of specific enterocytes from loss of intestinal
            LGR4 improves lipid metabolism in mice.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 951)
  AUTHORS   Gao,Y., Zhai,W., Sun,L., Du,X., Wang,X., Mulholland,M.W., Yin,Y.
            and Zhang,W.
  TITLE     Hepatic LGR4 aggravates cholestasis-induced liver injury in mice
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 326 (4), G460-G472 (2024)
   PUBMED   38440827
  REMARK    GeneRIF: Hepatic LGR4 aggravates cholestasis-induced liver injury
            in mice.
REFERENCE   5  (residues 1 to 951)
  AUTHORS   Watanabe,K., Horie,M., Hayatsu,M., Mikami,Y. and Sato,N.
  TITLE     Spatiotemporal expression patterns of R-spondins and their
            receptors, Lgrs, in the developing mouse telencephalon
  JOURNAL   Gene Expr Patterns 49, 119333 (2023)
   PUBMED   37651925
REFERENCE   6  (residues 1 to 951)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
REFERENCE   7  (residues 1 to 951)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   8  (residues 1 to 951)
  AUTHORS   Loh,E.D., Broussard,S.R. and Kolakowski,L.F.
  TITLE     Molecular characterization of a novel glycoprotein hormone
            G-protein-coupled receptor
  JOURNAL   Biochem Biophys Res Commun 282 (3), 757-764 (2001)
   PUBMED   11401528
REFERENCE   9  (residues 1 to 951)
  AUTHORS   Leighton,P.A., Mitchell,K.J., Goodrich,L.V., Lu,X., Pinson,K.,
            Scherz,P., Skarnes,W.C. and Tessier-Lavigne,M.
  TITLE     Defining brain wiring patterns and mechanisms through gene trapping
            in mice
  JOURNAL   Nature 410 (6825), 174-179 (2001)
   PUBMED   11242070
REFERENCE   10 (residues 1 to 951)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ093786.1, BE987673.1, CF164628.1, CN667965.1, AK033699.1,
            BU520309.1, CN456468.1, CA527305.1 and BE861913.1.
            
            On or before Feb 4, 2007 this sequence version replaced
            XP_922218.2, XP_355385.2, NP_766259.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 56.65 cM"
     Protein         1..951
                     /product="leucine-rich repeat-containing G-protein coupled
                     receptor 4 precursor"
                     /note="G protein-coupled receptor GPR48; G-protein coupled
                     receptor 48"
                     /calculated_mol_wt=101822
     sig_peptide     1..24
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2346
     mat_peptide     25..951
                     /product="Leucine-rich repeat-containing G-protein coupled
                     receptor 4. /id=PRO_0000303240"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
                     /calculated_mol_wt=101822
     Region          29..61
                     /region_name="LRRNT"
                     /note="Leucine rich repeat N-terminal domain; smart00013"
                     /db_xref="CDD:214470"
     Region          58..79
                     /region_name="LRR 1"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          59..82
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Site            68
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          <75..384
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          82..103
                     /region_name="LRR 2"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          83..106
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          106..127
                     /region_name="LRR 3"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          107..130
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          130..151
                     /region_name="LRR 4"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          131..154
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          154..177
                     /region_name="LRR 5"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          155..178
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          178..199
                     /region_name="LRR 6"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          179..202
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Site            188
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (A2ARI4.1)"
     Site            199
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          202..223
                     /region_name="LRR 7"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          203..226
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          226..247
                     /region_name="LRR 8"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          227..249
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          249..270
                     /region_name="LRR 9"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          250..273
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          273..294
                     /region_name="LRR 10"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          274..295
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Site            294
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          320..341
                     /region_name="LRR 11"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          321..344
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          344..365
                     /region_name="LRR 12"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          345..366
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          365..425
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          366..387
                     /region_name="LRR 13"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          367..390
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          390..411
                     /region_name="LRR 14"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          391..414
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          414..435
                     /region_name="LRR 15"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          415..438
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          487..512
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Site            505
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          539..812
                     /region_name="7tmA_LGR4"
                     /note="leucine-rich repeats-containing G protein-coupled
                     receptor 4, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd15361"
                     /db_xref="CDD:320483"
     Region          540..566
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320483"
     Site            545..565
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          573..598
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320483"
     Site            576..596
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Site            order(594,597..598,618..623,625..626,629,674,676..680,702,
                     705..707,709..711,713..714,760,763..764,766..767,770,
                     779..780,782..784,787,790..791)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320483"
     Region          618..648
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320483"
     Site            620..640
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          660..682
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320483"
     Site            662..682
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          702..731
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320483"
     Site            704..724
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          740..770
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320483"
     Site            757..777
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Region          780..805
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320483"
     Site            784..804
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (A2ARI4.1)"
     Site            920
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9BXB1; propagated from
                     UniProtKB/Swiss-Prot (A2ARI4.1)"
     CDS             1..951
                     /gene="Lgr4"
                     /gene_synonym="9130225G07; A330106J01Rik; A930009A08Rik;
                     Gpr48"
                     /coded_by="NM_172671.2:475..3330"
                     /db_xref="CCDS:CCDS38195.1"
                     /db_xref="GeneID:107515"
                     /db_xref="MGI:MGI:1891468"
ORIGIN      
        1 mpgplrllcf falgllgsag psgaapplca apcscdgdrr vdcsgkglta vpeglsaftq
       61 aldismnnit qlpedafknf pfleelqlag ndlsfihpka lsglkelkvl tlqnnqlktv
      121 pseairglsa lqslrldanh itsvpedsfe glvqlrhlwl ddniltevpv rplsnlptlq
      181 altlalnnis sipdfaftnl sslvvlhlhn nkikslsqhc fdgldnletl dlnynnldef
      241 pqaikalpsl kelgfhsnsi svipdgafag npllrtihly dnplsfvgns afhnlsdlhs
      301 lvirgaslvq wfpnlagtvh lesltltgtk issipddlcq nqkmlrtldl syndirdlps
      361 fngcraleei slqrnqisli kettfqglts lrildlsrnl ireihsgafa klgtitnldv
      421 sfneltsfpt eglnglnqlk lvgnfqlkda laardfanlr slsvpyayqc cafwgcdsya
      481 nlntednspq dhsvtkekga tdaanatsta eseehsqiii hctpstgafk pceyllgswm
      541 irltvwfifl vallfnllvi ltvfascssl pasklfigli svsnllmgiy tgiltfldav
      601 swgrfaefgi wwetgsgckv agslavfsse savflltlaa versvfakdv mkngksshlr
      661 qfqvaalval lgaaiagcfp lfhggqysas plclpfptge tpslgftvtl vllnslafll
      721 maiiytklyc nlekedpsen sqssmikhva wliftnciff cpvaffsfap litaisispe
      781 imksvtliff plpaclnpvl yvffnpkfkd dwkllkrrvt rkhgsvsvsi ssqggcgeqd
      841 fyydcgmysh lqgnltvcdc cesflltkpv sckhlikshs cpvltvascq rpeaywsdcg
      901 tqsahsdyad eedsfvsdss dqvqacgrac fyqsrgfplv ryaynlprvr d
//
