LOCUS       NM_011175               1921 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus legumain (Lgmn), transcript variant 1, mRNA.
ACCESSION   NM_011175
VERSION     NM_011175.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Zhang,W., Wang,S., Liu,Z., Qian,P., Li,Y. and Wu,J.
  TITLE     Legumain-deficient macrophages regulate inflammation and lipid
            metabolism in adipose tissues to protect against diet-induced
            obesity
  JOURNAL   Mol Cell Endocrinol 592, 112283 (2024)
   PUBMED   38815795
  REMARK    GeneRIF: Legumain-deficient macrophages regulate inflammation and
            lipid metabolism in adipose tissues to protect against diet-induced
            obesity.
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Wang,J., Yu,H., Li,X., Li,F., Chen,H., Zhang,X., Wang,Y., Xu,R.,
            Gao,F., Wang,J., Liu,P., Shi,Y., Qin,D., Li,Y., Liu,S., Ding,S.,
            Gao,X.Y. and Wang,Z.H.
  TITLE     A TrkB cleavage fragment in hippocampus promotes Depressive-Like
            behavior in mice
  JOURNAL   Brain Behav Immun 119, 56-83 (2024)
   PUBMED   38555992
REFERENCE   3  (bases 1 to 1921)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1921)
  AUTHORS   He,Y., Zou,P., Lu,J., Lu,Y., Yuan,S., Zheng,X., Liu,J., Zeng,C.,
            Liu,L., Tang,L., Fang,Z., Hu,X., Liu,Q. and Zhou,S.
  TITLE     CD4+ T-Cell Legumain Deficiency Attenuates Hypertensive Damage via
            Preservation of TRAF6
  JOURNAL   Circ Res 134 (1), 9-29 (2024)
   PUBMED   38047378
  REMARK    GeneRIF: CD4+ T-Cell Legumain Deficiency Attenuates Hypertensive
            Damage via Preservation of TRAF6.
REFERENCE   5  (bases 1 to 1921)
  AUTHORS   Pang,L., Guo,S., Khan,F., Dunterman,M., Ali,H., Liu,Y., Huang,Y.
            and Chen,P.
  TITLE     Hypoxia-driven protease legumain promotes immunosuppression in
            glioblastoma
  JOURNAL   Cell Rep Med 4 (11), 101238 (2023)
   PUBMED   37858339
  REMARK    GeneRIF: Hypoxia-driven protease legumain promotes
            immunosuppression in glioblastoma.
REFERENCE   6  (bases 1 to 1921)
  AUTHORS   Shirahama-Noda,K., Yamamoto,A., Sugihara,K., Hashimoto,N.,
            Asano,M., Nishimura,M. and Hara-Nishimura,I.
  TITLE     Biosynthetic processing of cathepsins and lysosomal degradation are
            abolished in asparaginyl endopeptidase-deficient mice
  JOURNAL   J Biol Chem 278 (35), 33194-33199 (2003)
   PUBMED   12775715
  REMARK    GeneRIF: AEP has a pivotal role in the endosomal/lysosomal
            degradation system
REFERENCE   7  (bases 1 to 1921)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   8  (bases 1 to 1921)
  AUTHORS   Chen,J.M., Rawlings,N.D., Stevens,R.A. and Barrett,A.J.
  TITLE     Identification of the active site of legumain links it to caspases,
            clostripain and gingipains in a new clan of cysteine endopeptidases
  JOURNAL   FEBS Lett 441 (3), 361-365 (1998)
   PUBMED   9891971
REFERENCE   9  (bases 1 to 1921)
  AUTHORS   Halfon,S., Patel,S., Vega,F., Zurawski,S. and Zurawski,G.
  TITLE     Autocatalytic activation of human legumain at aspartic acid
            residues
  JOURNAL   FEBS Lett 438 (1-2), 114-118 (1998)
   PUBMED   9821970
REFERENCE   10 (bases 1 to 1921)
  AUTHORS   Chen,J.M., Dando,P.M., Stevens,R.A., Fortunato,M. and Barrett,A.J.
  TITLE     Cloning and expression of mouse legumain, a lysosomal endopeptidase
  JOURNAL   Biochem J 335 (Pt 1) (Pt 1), 111-117 (1998)
   PUBMED   9742219
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC122302.4.
            
            On Mar 12, 2020 this sequence version replaced NM_011175.3.
            
            Summary: This gene encodes a member of the cysteine peptidase
            family C13 that plays an important role in the endosome/lysosomal
            degradation system. The encoded inactive preproprotein undergoes
            autocatalytic removal of the C-terminal inhibitory propeptide to
            generate the active endopeptidase that cleaves protein substrates
            on the C-terminal side of asparagine residues. Mice lacking the
            encoded protein exhibit defects in the lysosomal processing of
            proteins resulting in their accumulation in the lysosomes, and
            develop symptoms resembling hemophagocytic lymphohistiocytosis.
            [provided by RefSeq, Aug 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ000990.1, AF044266.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-98                AC122302.4         39080-39177
            99-269              AC122302.4         55053-55223
            270-367             AC122302.4         65246-65343
            368-449             AC122302.4         72931-73012
            450-535             AC122302.4         74463-74548
            536-611             AC122302.4         75306-75381
            612-674             AC122302.4         76076-76138
            675-741             AC122302.4         76857-76923
            742-860             AC122302.4         78552-78670
            861-950             AC122302.4         78814-78903
            951-1151            AC122302.4         80425-80625
            1152-1322           AC122302.4         82994-83164
            1323-1390           AC122302.4         83899-83966
            1391-1921           AC122302.4         84196-84726
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 51.45 cM"
     gene            1..1921
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /note="legumain"
                     /db_xref="GeneID:19141"
                     /db_xref="MGI:MGI:1330838"
     exon            1..98
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            99..269
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..1433
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /EC_number="3.4.22.34"
                     /note="asparaginyl endopeptidase; protease, cysteine, 1;
                     preprolegumain"
                     /codon_start=1
                     /product="legumain preproprotein"
                     /protein_id="NP_035305.1"
                     /db_xref="CCDS:CCDS26119.1"
                     /db_xref="GeneID:19141"
                     /db_xref="MGI:MGI:1330838"
                     /translation="MTWRVAVLLSLVLGAGAVPVGVDDPEDGGKHWVVIVAGSNGWYN
                     YRHQADACHAYQIIHRNGIPDEQIIVMMYDDIANSEENPTPGVVINRPNGTDVYKGVL
                     KDYTGEDVTPENFLAVLRGDAEAVKGKGSGKVLKSGPRDHVFIYFTDHGATGILVFPN
                     DDLHVKDLNKTIRYMYEHKMYQKMVFYIEACESGSMMNHLPDDINVYATTAANPKESS
                     YACYYDEERGTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKS
                     ISTMKVMQFQGMKHRASSPISLPPVTHLDLTPSPDVPLTILKRKLLRTNDVKESQNLI
                     GQIQQFLDARHVIEKSVHKIVSLLAGFGETAERHLSERTMLTAHDCYQEAVTHFRTHC
                     FNWHSVTYEHALRYLYVLANLCEAPYPIDRIEMAMDKVCLSHY"
     exon            270..367
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            368..449
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            450..535
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            536..611
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            612..674
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            675..741
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            742..860
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            861..950
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1151
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1322
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1390
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1921
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1871..1876
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /note="hexamer: AATAAT"
     polyA_site      1921
                     /gene="Lgmn"
                     /gene_synonym="AEP; Prsc1"
                     /note="major polyA site"
ORIGIN      
        1 aatcacagcg gggcggactc tagaggtggc tgctctgagt ctgcgcgacg cccggaattc
       61 ccacggttct gcagtcaccg cggcgatcac ccgcccagtc ttctgtagcg gacacggggt
      121 gcagaatgac ctggagagtg gctgtgcttc tcagcctggt gctgggtgct ggtgccgttc
      181 ccgtcggtgt ggacgatccc gaggatggag gcaagcactg ggtggtgatt gtggcgggct
      241 ccaatggctg gtataattac cgacaccagg cagacgcatg ccacgcctac cagatcatcc
      301 accggaacgg gattcctgac gagcagatca tagtgatgat gtatgacgac attgccaact
      361 ctgaagaaaa ccctacccca ggtgttgtga tcaaccgacc taacggcaca gatgtataca
      421 agggagtcct gaaggactac accggagagg atgtgactcc agagaatttc ctcgccgtgc
      481 tgagaggtga cgcagaagct gtgaagggca aagggtctgg aaaagtcttg aagagtggcc
      541 cccgagatca tgtcttcatt tacttcaccg accacggagc caccgggatc ctggtgtttc
      601 ctaatgatga tcttcatgtc aaggacctga ataagactat tcgctacatg tatgaacaca
      661 aaatgtacca gaagatggtg ttctacattg aagcttgtga gtctggctcc atgatgaacc
      721 acctgcccga cgacatcaac gtttatgcaa ctactgcggc caaccccaag gagtcatctt
      781 atgcctgcta ctacgacgag gagaggggca cttacctggg tgactggtac agcgtcaact
      841 ggatggaaga ctccgatgtg gaggacctga ccaaagagac ccttcacaag cagtaccacc
      901 tggtcaagtc ccacaccaac accagccatg tcatgcaata tgggaacaaa tctatctcta
      961 ccatgaaagt gatgcagttt cagggaatga agcacagagc cagttccccc atctccctgc
     1021 ctccggtcac acaccttgac ctcaccccca gccctgacgt gcccctgacc atcttgaaga
     1081 ggaagctgct gagaaccaac gacgtgaagg aatcccagaa tctcattggg cagatccagc
     1141 aatttctgga tgccaggcac gtcattgaga agtctgtgca caagatcgtt tccctgctgg
     1201 cgggatttgg ggaaactgct gagagacatc tgtcagagag gaccatgctc acagcacatg
     1261 actgctacca ggaggctgta acccacttcc gcacacactg ctttaactgg cactctgtca
     1321 cgtacgagca tgccttgcgg tacttgtatg tgctggccaa tctctgtgag gcaccatatc
     1381 cgattgacag gatagagatg gccatggaca aagtgtgtct tagtcactac tgaacagctc
     1441 gcttcccaat gagtgagcac agtccactgg aatatgaacc aaccagagac tgaaagggcg
     1501 gagccagagg cagcacccgc gcccctggcc cccaggaata ctgcccgccc accccagggc
     1561 ttgctttttg aagatacctg cttactaaga agccagtttg ggtgggtaaa gctctctgga
     1621 agaaggaact ttgcttctta ggagtttttt tgtttgtttg ttttggtttg ttttgttgtc
     1681 cattagcttt caagagcaaa ttccccgcgg cttctctagc cagggaagga atcgtctgag
     1741 aaattcaaag ctgaaacctc ttgccgtctt cacagtgatt tcactgaaga agagggtgga
     1801 aagcaagccc ctatgggaga attattttta gaattatata atttttgatt gcttttatat
     1861 tttattctgt aataatggat gtcttaaaac aaataagtga agtgagacat ttgtttctgg
     1921 a
//
