LOCUS       NM_001199043            2996 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus lectin, galactose binding, soluble 8 (Lgals8),
            transcript variant 1, mRNA.
ACCESSION   NM_001199043
VERSION     NM_001199043.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wang,Y., Sun,Y., Deng,S., Liu,J., Yu,J., Chi,H., Han,X., Zhang,Y.,
            Shi,J., Wang,Y., Quan,Y., Li,H. and Xu,J.
  TITLE     Discovery of galectin-8 as an LILRB4 ligand driving M-MDSCs defines
            a class of antibodies to fight solid tumors
  JOURNAL   Cell Rep Med 5 (1), 101374 (2024)
   PUBMED   38232701
  REMARK    GeneRIF: Discovery of galectin-8 as an LILRB4 ligand driving
            M-MDSCs defines a class of antibodies to fight solid tumors.
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Morrison,H.M., Craft,J., Rivera-Lugo,R., Johnson,J.R.,
            Golovkine,G.R., Bell,S.L., Dodd,C.E., Van Dis,E., Beatty,W.L.,
            Margolis,S.R., Repasy,T., Shaker,I., Lee,A.Y., Vance,R.E.,
            Stanley,S.A., Watson,R.O., Krogan,N.J., Portnoy,D.A., Penn,B.H. and
            Cox,J.S.
  TITLE     Deficiency in Galectin-3, -8, and -9 impairs immunity to chronic
            Mycobacterium tuberculosis infection but not acute infection with
            multiple intracellular pathogens
  JOURNAL   PLoS Pathog 19 (6), e1011088 (2023)
   PUBMED   37352334
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2996)
  AUTHORS   Shi,J., Chen,Y., Zhao,W., Chen,Y., Yang,Q., Zhao,Z., Wang,H.,
            Zhou,W. and Sun,W.
  TITLE     Galectin-8 involves in arthritic condylar bone loss via
            podoplanin/AKT/ERK axis-mediated inflammatory lymphangiogenesis
  JOURNAL   Osteoarthritis Cartilage 31 (6), 753-765 (2023)
   PUBMED   36702375
  REMARK    GeneRIF: Galectin-8 involves in arthritic condylar bone loss via
            podoplanin/AKT/ERK axis-mediated inflammatory lymphangiogenesis.
REFERENCE   4  (bases 1 to 2996)
  AUTHORS   Ramadan,A., Cao,Z., Hassan,M., Zetterberg,F., Nilsson,U.J.,
            Gadjeva,M., Rathinam,V. and Panjwani,N.
  TITLE     Galectin-8 Downmodulates TLR4 Activation and Impairs Bacterial
            Clearance in a Mouse Model of Pseudomonas aeruginosa Keratitis
  JOURNAL   J Immunol 210 (4), 398-407 (2023)
   PUBMED   36603009
  REMARK    GeneRIF: Galectin-8 Downmodulates TLR4 Activation and Impairs
            Bacterial Clearance in a Mouse Model of Pseudomonas aeruginosa
            Keratitis.
REFERENCE   5  (bases 1 to 2996)
  AUTHORS   Negi,V., Yang,J., Speyer,G., Pulgarin,A., Handen,A., Zhao,J.,
            Tai,Y.Y., Tang,Y., Culley,M.K., Yu,Q., Forsythe,P., Gorelova,A.,
            Watson,A.M., Al Aaraj,Y., Satoh,T., Sharifi-Sanjani,M.,
            Rajaratnam,A., Sembrat,J., Provencher,S., Yin,X., Vargas,S.O.,
            Rojas,M., Bonnet,S., Torrino,S., Wagner,B.K., Schreiber,S.L.,
            Dai,M., Bertero,T., Al Ghouleh,I., Kim,S. and Chan,S.Y.
  TITLE     Computational repurposing of therapeutic small molecules from
            cancer to pulmonary hypertension
  JOURNAL   Sci Adv 7 (43), eabh3794 (2021)
   PUBMED   34669463
REFERENCE   6  (bases 1 to 2996)
  AUTHORS   Tsai,C.M., Guan,C.H., Hsieh,H.W., Hsu,T.L., Tu,Z., Wu,K.J.,
            Lin,C.H. and Lin,K.I.
  TITLE     Galectin-1 and galectin-8 have redundant roles in promoting plasma
            cell formation
  JOURNAL   J Immunol 187 (4), 1643-1652 (2011)
   PUBMED   21753146
  REMARK    GeneRIF: a novel role for Gal-8 in collaboration with Gal-1 in
            plasma cell formation, and suggest the possibility of using
            distinct LacNAc ligands to modulate the function of Gals.
REFERENCE   7  (bases 1 to 2996)
  AUTHORS   Cattaneo,V., Tribulatti,M.V. and Campetella,O.
  TITLE     Galectin-8 tandem-repeat structure is essential for T-cell
            proliferation but not for co-stimulation
  JOURNAL   Biochem J 434 (1), 153-160 (2011)
   PUBMED   21091438
  REMARK    GeneRIF: The findings of the present study delineate the
            differential molecular requirements for Gal-8 activities on
            T-cells, and suggest a dual activity relying on activation state.
REFERENCE   8  (bases 1 to 2996)
  AUTHORS   Tribulatti,M.V., Cattaneo,V., Hellman,U., Mucci,J. and
            Campetella,O.
  TITLE     Galectin-8 provides costimulatory and proliferative signals to T
            lymphocytes
  JOURNAL   J Leukoc Biol 86 (2), 371-380 (2009)
   PUBMED   19401394
  REMARK    GeneRIF: Taken together, our findings support a distinctive role
            for locally produced Gal-8 as an enhancer of otherwise borderline
            immune responses and also suggest that Gal-8 might fuel the
            reactivity at inflammatory foci.
REFERENCE   9  (bases 1 to 2996)
  AUTHORS   Tribulatti,M.V., Mucci,J., Cattaneo,V., Aguero,F., Gilmartin,T.,
            Head,S.R. and Campetella,O.
  TITLE     Galectin-8 induces apoptosis in the CD4(high)CD8(high) thymocyte
            subpopulation
  JOURNAL   Glycobiology 17 (12), 1404-1412 (2007)
   PUBMED   17893094
  REMARK    GeneRIF: galectin-8 is expressed in the thymus in mouse, which
            suggests an active role for this lectin in shaping the mature T
            cell repertoire
REFERENCE   10 (bases 1 to 2996)
  AUTHORS   Martin,N., Jaubert,J., Glaser,P., Szatanik,M. and Guenet,J.L.
  TITLE     Genetic and physical delineation of the region overlapping the
            progressive motor neuropathy (pmn) locus on mouse chromosome 13
  JOURNAL   Genomics 75 (1-3), 9-16 (2001)
   PUBMED   11472062
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154221.3, AK004782.2 and BQ033229.1.
            
            Transcript Variant: This variant (1) differs in the 5' UTR and uses
            an alternate in-frame splice site in the 5' coding region, compared
            to variant 3. Variants 1 and 2 encode the same isoform (1), which
            is shorter than isoform 2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK004782.2, BC040243.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-319               AC154221.3         13577-13895
            320-2856            AK004782.2         2-2538
            2857-2996           BQ033229.1         1-140               c
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 4.64 cM"
     gene            1..2996
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /note="lectin, galactose binding, soluble 8"
                     /db_xref="GeneID:56048"
                     /db_xref="MGI:MGI:1928481"
     exon            1..498
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     exon            499..641
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     CDS             600..1550
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /note="isoform 1 is encoded by transcript variant 1;
                     galectin-8; galectin-8L; lectin, galactose binding,
                     soluble 5"
                     /codon_start=1
                     /product="galectin-8 isoform 1"
                     /protein_id="NP_001185972.1"
                     /db_xref="CCDS:CCDS36592.1"
                     /db_xref="GeneID:56048"
                     /db_xref="MGI:MGI:1928481"
                     /translation="MLSLNNLQNIIYNPIIPYVGTITEQLKPGSLIVIRGHVPKDSER
                     FQVDFQLGNSLKPRADVAFHFNPRFKRSSCIVCNTLTQEKWGWEEITYDMPFRKEKSF
                     EIVFMVLKNKFQVAVNGRHVLLYAHRISPEQIDTVGIYGKVNIHSIGFRFSSDLQSME
                     TSALGLTQINRENIQKPGKLQLSLPFEARLNASMGPGRTVVIKGEVNTNARSFNVDLV
                     AGKTRDIALHLNPRLNVKAFVRNSFLQDAWGEEERNITCFPFSSGMYFEMIIYCDVRE
                     FKVAINGVHSLEYKHRFKDLSSIDTLSVDGDIRLLDVRSW"
     exon            642..730
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     exon            731..941
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1061
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1118
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1145
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1234
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1400
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..2980
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2956..2961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /note="hexamer: ATTAAA"
     polyA_site      2980
                     /gene="Lgals8"
                     /gene_synonym="1200015E08Rik; D13Ertd524e; Lgals-8"
                     /note="major polyA site"
ORIGIN      
        1 ggctggaact acaggggtgc accccgaccg atttgtgcag agctgggtgg aaatcaacct
       61 gggggcttcc agcacgtgag gtgagccaca ccccaacccc agccagccac tcccacagcc
      121 agccataggt ctgcgtgtgt gacattaaga gcccaacagc cccagctcgc agcactcgga
      181 cgccccgccc ctgcagtcgt tctcgcggaa acggccgcgg gctgaccccg ccctctgcct
      241 gtggcggctc cgggaaccgg acggcgtccg tcaggggaca caagtcggag caaagcgctt
      301 actaccaccg gggtgagtcc tggcgactgc gggctcaggg acatcgggat accactgttc
      361 ccggaccgga atccgagtgt ggacacggca tcccgccgtc tcctccgtga cacatgcacg
      421 gggcaccgga caccgggagc gatcgtgggt tgaggcttgt actgggaccg tagtgtcggg
      481 acaacgctga ctgctgcgga caagttttta ctttgagtaa tccttaaatg aagagtgggt
      541 aaagtgtgta tacggaagag agactccaat caacaatatc aataagttga aaaagaaaaa
      601 tgttgtcctt aaataaccta caaaatatca tctataaccc gataatcccc tatgttggca
      661 ccattactga gcaattgaag cctggctctc tgattgtaat ccgtgggcat gtccctaaag
      721 attcagaaag attccaggtt gactttcagc tgggcaacag cctgaagcca agagcagacg
      781 tggccttcca ctttaaccct cggttcaaaa ggtctagctg cattgtttgt aacacactga
      841 cacaggagaa gtggggctgg gaggagatca cctacgacat gcccttcaga aaagaaaagt
      901 cctttgagat cgtgttcatg gtgctcaaga acaaattcca ggtggctgtg aacggaaggc
      961 atgttctgct gtacgcccac aggatcagcc cggagcagat cgacacagtg ggcatctacg
     1021 gcaaagtgaa catccactcc atcgggttca gattcagctc ggatttacag agtatggaaa
     1081 catctgctct gggactgaca cagataaaca gagagaatat acaaaagcca ggcaagctcc
     1141 agctgagcct gccatttgaa gcaaggttga atgcctccat gggtcctgga cgaaccgttg
     1201 tcattaaagg ggaagtgaac accaatgccc gaagctttaa tgttgaccta gtggcaggaa
     1261 aaacaaggga tatcgctctg cacttgaacc cacgcctcaa tgtgaaagca tttgtaagaa
     1321 attcctttct tcaggatgcc tggggagaag aggagagaaa tattacctgc ttcccattta
     1381 gttctgggat gtactttgag atgataatct actgtgatgt ccgggaattc aaggttgcta
     1441 taaatggtgt gcacagcctg gagtacaaac acagatttaa agacctaagc agtattgata
     1501 cactatcagt cgatggtgat atccgtttgc tggatgtaag gagctggtag ctaccatgac
     1561 tgccaaaacc cccgaaatac aaaatggctt atccggtact ggccatgtca aatgcatctc
     1621 gctttcacca tattgtttat attgctaagt tgagctcctc caacatcaag tcctactggt
     1681 gttgtcaggt ctggccatgc agtacattca gaggaacaga gccggggcaa tcacagctca
     1741 ctgccagaga ggctctgcac actgggtccc tcttataaac cacactcagc aaatatttaa
     1801 gtgcctaata tactacatat actagctaat agggatggca agcatacttc ctttgtatat
     1861 tctctgagcc gggcacagac atggcagggc ccagaacttg tgtggtccat gttttctagc
     1921 acttcgtacc agtttctggc ctcctaatgt agggtcttct tgctggcatt gcattaaccc
     1981 cactaggggc ctttgcagtt aaggtcagaa aaatatacta atggatggca aacactactt
     2041 ccccagcaac ccttttcata atcagcattc tatcatatct cataattgaa gactgcatag
     2101 catttactta gctctcaccg ctttaaactt tataaaatgt atgatgctga acacagcaga
     2161 aaaactgagg ccaaaaccct gaattatgac aaaacaagtg ttctgctcca agcagatttc
     2221 tgctggttga ttggcgctca agtccagggt gtgtgggtac ctgtggcaaa gtaaggcaga
     2281 agttggataa accgtgtgtg taaaaccctc ttggacgtat atataaaaca agcactatca
     2341 aagcaaaccc aggggcgtag tgtgaaaggc ttagttggtg tggagactag ccctcgtgcc
     2401 tttgggtctg aagatgtcgg tctgcagcag cagcgaggtc aggcacatta aaccacagga
     2461 cagaattctg ctggggttta ggagtattca gccaatctgc ttagatatat gcacttgtgc
     2521 atatgaaata ataccatcag cagtcttact caaggcagca actgctagtc cttcattttc
     2581 atgcaaattt attatgttca ctcttcaata tggggtggtg ggtggaactg ggtaatttgt
     2641 acggaggcca ggaggctcta gaattctcca gagttatgtc ttcataaaga atgagtcttc
     2701 ataaagaatg cactactgag atattggggg ctcaaaggca ctcaggaaaa aaaaataaaa
     2761 gcctattcat acaacagctt attttcattt ctatttttac acaattaaga ctgattctaa
     2821 gtagattcag tcttaagttc ttagattttt tttttttaaa aagctgctgg aatttaggtt
     2881 gtgagacctt ctgttgtata ttccgaaatt ctatctctaa actgcaaaat gcctttttgc
     2941 ttgtcctaat tctgcattaa agttttatat taaattggaa aaaaaaaaaa aaaaaa
//
