LOCUS       NM_146075               2603 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus LEM domain containing 2 (Lemd2), mRNA.
ACCESSION   NM_146075
VERSION     NM_146075.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Ross,J.A., Arcos-Villacis,N., Battey,E., Boogerd,C., Orellana,C.A.,
            Marhuenda,E., Swiatlowska,P., Hodzic,D., Prin,F., Mohun,T.,
            Catibog,N., Tapia,O., Gerace,L., Iskratsch,T., Shah,A.M. and
            Stroud,M.J.
  TITLE     Lem2 is essential for cardiac development by maintaining nuclear
            integrity
  JOURNAL   Cardiovasc Res 119 (11), 2074-2088 (2023)
   PUBMED   37067297
  REMARK    GeneRIF: Lem2 is essential for cardiac development by maintaining
            nuclear integrity.
REFERENCE   3  (bases 1 to 2603)
  AUTHORS   Chen,R., Buchmann,S., Kroth,A., Arias-Loza,A.P., Kohlhaas,M.,
            Wagner,N., Gruner,G., Nickel,A., Cirnu,A., Williams,T., Maack,C.,
            Ergun,S., Frantz,S. and Gerull,B.
  TITLE     Mechanistic Insights of the LEMD2 p.L13R Mutation and Its Role in
            Cardiomyopathy
  JOURNAL   Circ Res 132 (2), e43-e58 (2023)
   PUBMED   36656972
  REMARK    GeneRIF: Mechanistic Insights of the LEMD2 p.L13R Mutation and Its
            Role in Cardiomyopathy.
REFERENCE   4  (bases 1 to 2603)
  AUTHORS   Caravia,X.M., Ramirez-Martinez,A., Gan,P., Wang,F., McAnally,J.R.,
            Xu,L., Bassel-Duby,R., Liu,N. and Olson,E.N.
  TITLE     Loss of function of the nuclear envelope protein LEMD2 causes DNA
            damage-dependent cardiomyopathy
  JOURNAL   J Clin Invest 132 (22), e158897 (2022)
   PUBMED   36377660
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2603)
  AUTHORS   Ramirez-Martinez,A., Zhang,Y., Chen,K., Kim,J., Cenik,B.K.,
            McAnally,J.R., Cai,C., Shelton,J.M., Huang,J., Brennan,A.,
            Evers,B.M., Mammen,P.P.A., Xu,L., Bassel-Duby,R., Liu,N. and
            Olson,E.N.
  TITLE     The nuclear envelope protein Net39 is essential for muscle nuclear
            integrity and chromatin organization
  JOURNAL   Nat Commun 12 (1), 690 (2021)
   PUBMED   33514739
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2603)
  AUTHORS   Thanisch,K., Song,C., Engelkamp,D., Koch,J., Wang,A., Hallberg,E.,
            Foisner,R., Leonhardt,H., Stewart,C.L., Joffe,B. and Solovei,I.
  TITLE     Nuclear envelope localization of LEMD2 is developmentally dynamic
            and lamin A/C dependent yet insufficient for heterochromatin
            tethering
  JOURNAL   Differentiation 94, 58-70 (2017)
   PUBMED   28056360
  REMARK    GeneRIF: we expressed a LEMD2 transgene alone or in combination
            with lamin C in these cells and observed no restoration of
            peripheral heterochromatin in either case. We conclude that in
            contrary to the B-tether, the A-tether has a more intricate
            composition and consists of multiple components that presumably
            vary, at differing degrees of redundancy, between cell types and
            differentiation stages
REFERENCE   7  (bases 1 to 2603)
  AUTHORS   Tapia,O., Fong,L.G., Huber,M.D., Young,S.G. and Gerace,L.
  TITLE     Nuclear envelope protein Lem2 is required for mouse development and
            regulates MAP and AKT kinases
  JOURNAL   PLoS One 10 (3), e0116196 (2015)
   PUBMED   25790465
  REMARK    GeneRIF: Lemd2 plays an essential role in mouse embryonic
            development and that it is involved in regulating several signaling
            pathways.
            GeneRIF: Lemd2 is required for embryonic development in mouse and
            its loss is associated with highly elevated activity of AKT and MAP
            kinases.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2603)
  AUTHORS   Huber,M.D., Guan,T. and Gerace,L.
  TITLE     Overlapping functions of nuclear envelope proteins NET25 (Lem2) and
            emerin in regulation of extracellular signal-regulated kinase
            signaling in myoblast differentiation
  JOURNAL   Mol Cell Biol 29 (21), 5718-5728 (2009)
   PUBMED   19720741
  REMARK    GeneRIF: Deregulation of cell signaling contributes to nuclear
            envelope-linked disorders and suggests that mutations in NET25 and
            MAN1 may cause muscle diseases.
REFERENCE   9  (bases 1 to 2603)
  AUTHORS   Brachner,A., Reipert,S., Foisner,R. and Gotzmann,J.
  TITLE     LEM2 is a novel MAN1-related inner nuclear membrane protein
            associated with A-type lamins
  JOURNAL   J Cell Sci 118 (Pt 24), 5797-5810 (2005)
   PUBMED   16339967
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY732449.1, AK143224.1, AK082131.1 and AA259360.1.
            
            On Jan 19, 2008 this sequence version replaced NM_146075.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK143224.1, AK082131.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                BY732449.1         2-17
            17-2438             AK143224.1         2-2423
            2439-2590           AK082131.1         2247-2398
            2591-2603           AA259360.1         242-254
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 13.82 cM"
     gene            1..2603
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /note="LEM domain containing 2"
                     /db_xref="GeneID:224640"
                     /db_xref="MGI:MGI:2385045"
     exon            1..780
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..1556
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /note="nuclear envelope constituent; nuclear envelope
                     transmembrane protein 25"
                     /codon_start=1
                     /product="LEM domain-containing protein 2"
                     /protein_id="NP_666187.2"
                     /db_xref="CCDS:CCDS50043.1"
                     /db_xref="GeneID:224640"
                     /db_xref="MGI:MGI:2385045"
                     /translation="MAGLSDLELRRELQALGFQPGPITDTTRNVYRNKLRRLRGEARL
                     RDDERLREDAGPREDAGPRGPERQREEARLREEAPLRARPAASVLRSEPWPLSPSPPA
                     PSAASDASGPYGNFGASASPWAASRGLSYPPHAGPGPLRRRASVRGSSEDDEDTRTPD
                     RHAPGRGRHWWAPPSASARPHSALLGADARPGLKGSRTGSAGAGRTRPEVGRWLERCL
                     SRLLLWASLGLLLGFLAILWVKMGKPSAPQEAEDNMKLLPVDCERKTDEFCQAKQKAA
                     LLELLHELYNFLAIQAGNFECGNPEKLKSKCIPVLEAQEYIANVTSSPSSRFKAALTW
                     ILSSNKDVGIWLKGEDPSELATTVDKVVCLESARPRMGIGCRLSRALLTAVTHVLIFF
                     WCLAFLWGLLILLKYRWRKLEEEEQAMYEMVKKIIDVVQDHYVDWEQDMERYPYVGIL
                     HVRDSLIPPQSRRRMKRVWDRAVEFLASNESRIQTESHRVAGEDMLVWRWTKPSSFSD
                     SER"
     exon            781..821
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     exon            822..897
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     exon            898..974
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     exon            975..1054
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1200
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1302
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1405
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..2596
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2418..2423
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /note="hexamer: AATAAA"
     polyA_site      2439
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /note="major polyA site"
     regulatory      2573..2578
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /note="hexamer: AATAAA"
     polyA_site      2596
                     /gene="Lemd2"
                     /gene_synonym="Lem2; NET25"
                     /note="major polyA site"
ORIGIN      
        1 gagggggcgg tgtcctggcc atggccggcc tgtcggacct ggagttgcgg cgagagctgc
       61 aggccctggg cttccagcca ggccccatca ccgacaccac gcggaacgtc taccgcaaca
      121 agctgcgccg cctgcggggc gaggcccggc tgcgcgacga cgagcggctg cgggaggacg
      181 ccgggccgcg ggaggacgcc gggccgcggg gccccgagcg gcagcgggag gaggcccggc
      241 tacgcgagga agcgccgctg cgcgcgcggc ccgccgccag cgtcctgcgc tcggagccct
      301 ggccgctgtc gccttccccg ccggcgccta gcgcggcctc cgacgcctcg gggccgtacg
      361 gcaacttcgg ggcctccgcc tctccctggg ccgcgagccg cggcctctcc tacccgcccc
      421 acgccgggcc cgggccgctg cgacgccgcg cctcggtccg gggcagctcg gaggatgacg
      481 aggatacacg aacgccggac aggcacgccc cgggccgggg ccgccactgg tgggccccgc
      541 cgtcggcctc tgcgcggccg cactcggcgc tcctcggcgc cgacgctcgc cccggcctga
      601 agggctcgcg caccggctcg gcgggcgccg ggcggacccg acccgaggtg ggccggtggc
      661 tggagcgctg cctgtctcgg ctcctgctct gggccagcct ggggctgctg ctcggcttct
      721 tggccatcct gtgggtgaag atgggcaagc cctcggcgcc gcaggaggcg gaggacaaca
      781 tgaaattgtt gccggtcgac tgtgagagaa aaacagatga gttctgccag gccaagcaga
      841 aggcggccct gttggagctg ctgcacgaac tgtacaactt cctggccatc caggcaggta
      901 attttgaatg tggcaacccg gagaagctga agagcaagtg catcccagtc ctggaggcgc
      961 aggagtacat agctaatgtg accagcagtc cctcttcgag gtttaaggct gcgctgacct
     1021 ggatcctgag cagcaacaag gacgtgggca tctggctgaa aggggaagac ccatctgagc
     1081 tggcgactac agtggacaaa gtggtctgcc tggagtccgc ccggccccgg atgggcatag
     1141 gctgccgcct cagccgcgcc ctgctcaccg ctgtcaccca cgtgctcatc ttcttctggt
     1201 gcctggcctt cctgtggggg ctgctgatcc tcctgaagta ccgctggcgg aagctggagg
     1261 aggaggagca ggccatgtat gagatggtga agaagatcat agatgtggtt caggaccact
     1321 atgtggactg ggaacaggac atggagcgct acccctacgt gggcatcctc cacgtgcggg
     1381 acagtctaat ccccccacag agccggcggc gcatgaagcg agtatgggac cgtgctgtgg
     1441 aattcctggc ttccaatgaa tctcggatcc agactgagtc ccaccgagtg gccggggagg
     1501 acatgctggt gtggagatgg actaagcctt cctctttctc tgattcagag cgatagggca
     1561 gggcctgtta ccgccagcct gacccagcca cccagctggg gcaaggacac agacctttgg
     1621 tgctcattca cacctgcctt gattgccctc cgggtctcag gtctggaaca tgtgggctta
     1681 cttagggtag ccgggagaac aggggagccc ttcctctatg tcagtattac tgactgacag
     1741 gggagagtgg cttgttctcc ctggataaaa agatgggcag tggttggaac cctggaggtc
     1801 ctgcacacag ctagacagca cggtgatcct gaagcccctc gcttggaagg tgggttgggt
     1861 ggtggggcaa agcttggtcc atgtgccttt tcgcttccag ggagcttaag gggaggggag
     1921 gaacccagcc cactcctgca tgggcctctg tccagaaggg gacgatgtta tagaaggtag
     1981 gagcagctgg aggggaccaa gcagaggtgg ctggggtctg gaaggcgtgg ggagtccagc
     2041 cacagctgcc tgtgcctcag agctgctcac aggtcagcag tgccacctga gccagggtgg
     2101 agctcagcct cctcacctac ttactgtgca caggcttcct ggctgcttag ccacacaggt
     2161 agggtgcagc atcgtggctg cacactgccc tctgcaggcc acatggagta gtgtgggcca
     2221 ttagccctcg gctatggagg acctgctcta aatacaccca gtggggggcc tcattagctg
     2281 agagggaggc tttcgagcaa ctcttcaggc gggtctcacc tacagcagca ggaggacagc
     2341 ccctggaggt gacgtgcagc tgcctccaga agacacaaga cacccccttt tggacctatt
     2401 gctggtctct gtttcttaat aaagtgtggt ctccatgtag cagtatgctc atttcggtct
     2461 ccgtgtagca gtatgctcat tgccgtgtgt ctgggtggtc agcaggaccg ggactgagca
     2521 ggccccagcc agaagacaat ggcttttgta ttgggggtgg aactgttgga aaaataaaat
     2581 atttattgag aacggaaaaa aaa
//
