LOCUS       NM_177099               2494 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus left-right determination factor 2 (Lefty2), mRNA.
ACCESSION   NM_177099 XM_976364
VERSION     NM_177099.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Chen,X., Su,Q., Ling,X., Yang,Y., Liu,Y., Zhu,X., He,A., Wu,H. and
            Qi,Y.
  TITLE     SENP3-regulated Nodal signaling plays a potential role in cardiac
            left-right asymmetry development
  JOURNAL   Int J Biol Macromol 274 (Pt 2), 133294 (2024)
   PUBMED   38925188
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Wang,R., Yang,X., Chen,J., Zhang,L., Griffiths,J.A., Cui,G.,
            Chen,Y., Qian,Y., Peng,G., Li,J., Wang,L., Marioni,J.C., Tam,P.P.L.
            and Jing,N.
  TITLE     Time space and single-cell resolved tissue lineage trajectories and
            laterality of body plan at gastrulation
  JOURNAL   Nat Commun 14 (1), 5675 (2023)
   PUBMED   37709743
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2494)
  AUTHORS   Qu,F., Li,W., Xu,J., Zhang,R., Ke,J., Ren,X., Meng,X., Qin,L.,
            Zhang,J., Lu,F., Zhou,X., Luo,X., Zhang,Z., Wang,M., Wu,G., Pei,D.,
            Chen,J., Cui,G., Suo,S. and Peng,G.
  TITLE     Three-dimensional molecular architecture of mouse organogenesis
  JOURNAL   Nat Commun 14 (1), 4599 (2023)
   PUBMED   37524711
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2494)
  AUTHORS   Ajima,R., Sakakibara,Y., Sakurai-Yamatani,N., Muraoka,M. and
            Saga,Y.
  TITLE     Formal proof of the requirement of MESP1 and MESP2 in mesoderm
            specification and their transcriptional control via specific
            enhancers in mice
  JOURNAL   Development 148 (20) (2021)
   PUBMED   34679163
REFERENCE   5  (bases 1 to 2494)
  AUTHORS   Nguyen,D.H., Soygur,B., Peng,S.P., Malki,S., Hu,G. and Laird,D.J.
  TITLE     Apoptosis in the fetal testis eliminates developmentally defective
            germ cell clones
  JOURNAL   Nat Cell Biol 22 (12), 1423-1435 (2020)
   PUBMED   33199844
REFERENCE   6  (bases 1 to 2494)
  AUTHORS   Takeda,S., Yonekawa,Y., Tanaka,Y., Okada,Y., Nonaka,S. and
            Hirokawa,N.
  TITLE     Left-right asymmetry and kinesin superfamily protein KIF3A: new
            insights in determination of laterality and mesoderm induction by
            kif3A-/- mice analysis
  JOURNAL   J Cell Biol 145 (4), 825-836 (1999)
   PUBMED   10330409
REFERENCE   7  (bases 1 to 2494)
  AUTHORS   Nonaka,S., Tanaka,Y., Okada,Y., Takeda,S., Harada,A., Kanai,Y.,
            Kido,M. and Hirokawa,N.
  TITLE     Randomization of left-right asymmetry due to loss of nodal cilia
            generating leftward flow of extraembryonic fluid in mice lacking
            KIF3B motor protein
  JOURNAL   Cell 95 (6), 829-837 (1998)
   PUBMED   9865700
  REMARK    Erratum:[Cell 1999 Oct 1;99(1):117]
REFERENCE   8  (bases 1 to 2494)
  AUTHORS   Meno,C., Shimono,A., Saijoh,Y., Yashiro,K., Mochida,K., Ohishi,S.,
            Noji,S., Kondoh,H. and Hamada,H.
  TITLE     lefty-1 is required for left-right determination as a regulator of
            lefty-2 and nodal
  JOURNAL   Cell 94 (3), 287-297 (1998)
   PUBMED   9708731
REFERENCE   9  (bases 1 to 2494)
  AUTHORS   Dufort,D., Schwartz,L., Harpal,K. and Rossant,J.
  TITLE     The transcription factor HNF3beta is required in visceral endoderm
            for normal primitive streak morphogenesis
  JOURNAL   Development 125 (16), 3015-3025 (1998)
   PUBMED   9671576
REFERENCE   10 (bases 1 to 2494)
  AUTHORS   Meno,C., Ito,Y., Saijoh,Y., Matsuda,Y., Tashiro,K., Kuhara,S. and
            Hamada,H.
  TITLE     Two closely-related left-right asymmetrically expressed genes,
            lefty-1 and lefty-2: their distinct expression domains, chromosomal
            linkage and direct neuralizing activity in Xenopus embryos
  JOURNAL   Genes Cells 2 (8), 513-524 (1997)
   PUBMED   9348041
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK031621.1 and BE994648.1.
            
            On Sep 1, 2015 this sequence version replaced NM_177099.3.
            
            Summary: This gene encodes a secreted ligand of the TGF-beta
            (transforming growth factor-beta) superfamily of proteins. Ligands
            of this family bind various TGF-beta receptors leading to
            recruitment and activation of SMAD family transcription factors
            that regulate gene expression. The encoded preproprotein is
            proteolytically processed to generate the mature protein, which
            plays a role in left-right asymmetry determination of organ systems
            during development. This protein is also important in self-renewal
            and differentiation of mouse embryonic stem cells. Mice lacking a
            functional copy of this gene exhibit defects in axial and
            left-right patterning. [provided by RefSeq, Aug 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066224.1, CV736182.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2160              AK031621.1         2-2161
            2161-2494           BE994648.1         1-334               c
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 84.46 cM"
     gene            1..2494
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /note="left-right determination factor 2"
                     /db_xref="GeneID:320202"
                     /db_xref="MGI:MGI:2443573"
     exon            1..326
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..1183
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /note="endometrial bleeding associated factor; left-right
                     determination factor B; left-right determination, factor
                     A"
                     /codon_start=1
                     /product="left-right determination factor 2 preproprotein"
                     /protein_id="NP_796073.1"
                     /db_xref="CCDS:CCDS15575.1"
                     /db_xref="GeneID:320202"
                     /db_xref="MGI:MGI:2443573"
                     /translation="MKSLWLCWALWVLPLAGPGAAMTEEQVLSSLLQQLQLSQAPTLD
                     SADVEEMAIPTHVRSQYVALLQGSHADRSRGKRFSQNFREVAGRFLMSETSTHLLVFG
                     MEQRLPPNSELVQAVLRLFQEPVPRTALRRFERLSPHSARARVTIEWLRVREDGSNRT
                     ALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPGTWSAHKL
                     VRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEVPVTEGTRCCRQEMYLDLQG
                     MKWAENWILEPPGFLTYECVGSCLQLPESLTIGWPFLGPRQCVASEMTSLPMIVSVKE
                     GGRTRPQVVSLPNMRVQTCSCASDGALIPRGIDL"
     exon            327..573
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /inference="alignment:Splign:2.1.0"
     exon            574..819
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /inference="alignment:Splign:2.1.0"
     exon            820..2477
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1814
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /note="major polyA site"
     regulatory      2409..2414
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /note="hexamer: ATTATA"
     polyA_site      2432
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /note="major polyA site"
     regulatory      2450..2455
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /note="hexamer: AATAAA"
     polyA_site      2469
                     /gene="Lefty2"
                     /gene_synonym="6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2;
                     lefty-B"
                     /note="major polyA site"
ORIGIN      
        1 agaccgtcta ggtcccaaga acttttcagg gcacttttag ggacgcatat atccacgatt
       61 cctcctgggc agcgccatga agtccctgtg gctttgctgg gcactctggg tactgcccct
      121 ggctggccct ggggcagcga tgaccgagga acaggtcctg agcagtctac tgcagcagct
      181 gcagctcagc caggccccca ccctggacag cgcggatgtg gaggagatgg ccatccctac
      241 ccacgtgagg tcccagtatg tggccctgct gcagggaagt cacgctgacc gctcccgagg
      301 caagaggttc agccagaatt ttcgagaggt ggcaggcagg ttcctgatgt cagagacctc
      361 cactcacctg ctagtgttcg gaatggagca gcggctgccg cctaacagcg agctggtgca
      421 ggctgtgctg cggctgttcc aggagcctgt gcccagaaca gctctccgga ggtttgagag
      481 gctgtcccca cacagtgccc gggctcgggt caccattgaa tggctgagag tccgtgagga
      541 tggctccaat cgcactgccc tcatcgactc taggctcgtg tccatccacg agagcggctg
      601 gaaggccttc gacgtgaccg aggccgtgaa cttctggcag cagctgagcc ggccgaggca
      661 gccgctgctg ctccaggtgt cggtgcagag ggagcatctg gggccgggga cctggagcgc
      721 acacaagttg gtccgtttcg cggcgcaggg gacgccggac ggcaaggggc agggcgagcc
      781 acagctggag ctgcacacgc tggacctcaa ggactacgga gctcaaggca attgtgaccc
      841 cgaggtacca gtgactgaag gcacccgatg ctgtcgccag gagatgtacc tggacctgca
      901 ggggatgaag tgggccgaga actggatcct agaaccgcca gggttcctga cgtatgaatg
      961 tgtgggcagc tgcctgcagc taccagagtc cctgaccatc gggtggccat ttctggggcc
     1021 tcggcagtgt gttgcctcag agatgacctc cttgcccatg attgtcagtg tgaaggaggg
     1081 aggcaggacc aggcctcaag tggtcagcct gcccaacatg agggtgcaga cctgtagctg
     1141 cgcctcagat ggggcgctca tacccagggg gatagatctg tagtctccct gtccacagat
     1201 gtattctcag tgagcttgtc ctaacttagt gctctcgtca gacctttgct ctacagtctt
     1261 ggttttcttg tccatcaccc agtttaagca cttacatggg taaatcatgt cactccagta
     1321 ggacacactg accccactta gccaaggaca tggctatgca gtgaacaggt tcgcatctga
     1381 gtctgttttc tggccagaac tcagcttaat gtacaacaaa accctacggt gagaacaggg
     1441 gaatcaaaag ctcgtttact cttacaccgt gattactggc atcaacgtac catgtcaggg
     1501 actgcccaca gcaggctggg agggagacat ctcagaagcc tgcggcagct ccttgtgaaa
     1561 aaccgttgtt cccatttctc ctaaccttag ccctagacaa gagctgtata gatttcatgt
     1621 gtgtgactgc ttttcagttg gccttggtgt tcatagttat tctatattat ttgactttcc
     1681 tactcctttc tccttctgcc ctggtgaatt ctatgaaact agatgttcct tgatgtaatg
     1741 attcttaaac aattaaaaag ttgaggcatg ggacacagca cagcacagtc ctgatggccc
     1801 aggtgcatgc tgtagatgta ttctgtgtgc tcttatcttg gaaacaatgc aataactttg
     1861 caatgttagt tcagattaat gtttgacttg caaagaaagt ttgaagaaat tattagaaag
     1921 tgaaatagag ccaacactgg gatcccgaaa agaaaaaagc tattgaagtt atgaaataag
     1981 ttttgcacaa aatttgagag tgtttcctgg ataagcaagt atagaataca taaaatctta
     2041 tattagtaaa actaagccaa aacaccggga ctcttaggag ggtcactgcg tgcaatgtgc
     2101 agaagcagaa agctggcaga actgccgagt taagggtgta cctgagtctt tctggccatt
     2161 gcctggcagc tttgcccatg tcatttattg tcagagcttc acgggaaaat gcaagtagcc
     2221 gacttcggag ctctgagctc tggagtataa taagtcaaaa ggtaaagttt aaataatgat
     2281 aagtttgcaa taattattat tttggccaga ggcctgggaa taggggaagc ttgaaactct
     2341 gggggaacaa ttataattct tgattctttg tgtgatgtgg gtattgtttt gaatttgatt
     2401 tggcaacgat tatacaatgt ctttttttcc tatctgcatt tggagtatca ataaaagact
     2461 ggggcaagag aaagggaaaa aaaaaaaaaa aaaa
//
