LOCUS       NM_010094               1639 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus left right determination factor 1 (Lefty1), mRNA.
ACCESSION   NM_010094
VERSION     NM_010094.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Weberling,A., Siriwardena,D., Penfold,C., Christodoulou,N.,
            Boroviak,T.E. and Zernicka-Goetz,M.
  TITLE     Primitive to visceral endoderm maturation is essential for mouse
            epiblast survival beyond implantation
  JOURNAL   iScience 28 (1), 111671 (2024)
   PUBMED   39868030
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Chen,X., Su,Q., Ling,X., Yang,Y., Liu,Y., Zhu,X., He,A., Wu,H. and
            Qi,Y.
  TITLE     SENP3-regulated Nodal signaling plays a potential role in cardiac
            left-right asymmetry development
  JOURNAL   Int J Biol Macromol 274 (Pt 2), 133294 (2024)
   PUBMED   38925188
REFERENCE   3  (bases 1 to 1639)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1639)
  AUTHORS   Yang,Y., Wang,X., Tan,Y., Xu,Y., Guo,X., Wu,Y., Wang,W., Jing,R.,
            Zhu,F., Ye,D., Zhang,Q., Lu,C., Kang,J. and Wang,G.
  TITLE     LncCMRR Plays an Important Role in Cardiac Differentiation by
            Regulating the Purb/Flk1 Axis
  JOURNAL   Stem Cells 41 (1), 11-25 (2023)
   PUBMED   36318802
REFERENCE   5  (bases 1 to 1639)
  AUTHORS   Ichikawa,T., Zhang,H.T., Panavaite,L., Erzberger,A., Fabreges,D.,
            Snajder,R., Wolny,A., Korotkevich,E., Tsuchida-Straeten,N.,
            Hufnagel,L., Kreshuk,A. and Hiiragi,T.
  TITLE     An ex vivo system to study cellular dynamics underlying mouse
            peri-implantation development
  JOURNAL   Dev Cell 57 (3), 373-386 (2022)
   PUBMED   35063082
REFERENCE   6  (bases 1 to 1639)
  AUTHORS   Heymer,J., Kuehn,M. and Ruther,U.
  TITLE     The expression pattern of nodal and lefty in the mouse mutant Ft
            suggests a function in the establishment of handedness
  JOURNAL   Mech Dev 66 (1-2), 5-11 (1997)
   PUBMED   9376323
REFERENCE   7  (bases 1 to 1639)
  AUTHORS   Meno,C., Ito,Y., Saijoh,Y., Matsuda,Y., Tashiro,K., Kuhara,S. and
            Hamada,H.
  TITLE     Two closely-related left-right asymmetrically expressed genes,
            lefty-1 and lefty-2: their distinct expression domains, chromosomal
            linkage and direct neuralizing activity in Xenopus embryos
  JOURNAL   Genes Cells 2 (8), 513-524 (1997)
   PUBMED   9348041
REFERENCE   8  (bases 1 to 1639)
  AUTHORS   Meno,C., Saijoh,Y., Fujii,H., Ikeda,M., Yokoyama,T., Yokoyama,M.,
            Toyoda,Y. and Hamada,H.
  TITLE     Left-right asymmetric expression of the TGF beta-family member
            lefty in mouse embryos
  JOURNAL   Nature 381 (6578), 151-155 (1996)
   PUBMED   8610011
REFERENCE   9  (bases 1 to 1639)
  AUTHORS   Bouillet,P., Oulad-Abdelghani,M., Vicaire,S., Garnier,J.M.,
            Schuhbaur,B., Dolle,P. and Chambon,P.
  TITLE     Efficient cloning of cDNAs of retinoic acid-responsive genes in P19
            embryonal carcinoma cells and characterization of a novel mouse
            gene, Stra1 (mouse LERK-2/Eplg2)
  JOURNAL   Dev Biol 170 (2), 420-433 (1995)
   PUBMED   7649373
REFERENCE   10 (bases 1 to 1639)
  AUTHORS   Cereghini,S., Ott,M.O., Power,S. and Maury,M.
  TITLE     Expression patterns of vHNF1 and HNF1 homeoproteins in early
            postimplantation embryos suggest distinct and sequential
            developmental roles
  JOURNAL   Development 116 (3), 783-797 (1992)
   PUBMED   1363228
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC117673.18, AK131943.1 and
            AA216877.1.
            
            On Sep 1, 2016 this sequence version replaced NM_010094.3.
            
            Summary: This gene encodes a secreted ligand of the TGF-beta
            (transforming growth factor-beta) superfamily of proteins. Ligands
            of this family bind various TGF-beta receptors leading to
            recruitment and activation of SMAD family transcription factors
            that regulate gene expression. The encoded preproprotein is
            proteolytically processed to generate the mature protein, which
            plays a role in left-right asymmetry determination of organ systems
            during development. Mice lacking a functional copy of this gene
            exhibit embryonic lethality and defects in left-right patterning.
            [provided by RefSeq, Aug 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050221.1, D83921.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AC117673.18        85430-85446
            18-1620             AK131943.1         3-1605
            1621-1639           AA216877.1         354-372
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 84.46 cM"
     gene            1..1639
                     /gene="Lefty1"
                     /gene_synonym="Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4"
                     /note="left right determination factor 1"
                     /db_xref="GeneID:13590"
                     /db_xref="MGI:MGI:107405"
     exon            1..338
                     /gene="Lefty1"
                     /gene_synonym="Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..1195
                     /gene="Lefty1"
                     /gene_synonym="Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4"
                     /note="left-right determination, factor B; TGF-beta-4;
                     protein lefty-1; transforming growth factor beta-4;
                     stimulated by retinoic acid gene 3 protein"
                     /codon_start=1
                     /product="left-right determination factor 1 preproprotein"
                     /protein_id="NP_034224.1"
                     /db_xref="CCDS:CCDS15577.1"
                     /db_xref="GeneID:13590"
                     /db_xref="MGI:MGI:107405"
                     /translation="MPFLWLCWALWALSLVSLREALTGEQILGSLLQQLQLDQPPVLD
                     KADVEGMVIPSHVRTQYVALLQHSHASRSRGKRFSQNLREVAGRFLVSETSTHLLVFG
                     MEQRLPPNSELVQAVLRLFQEPVPRTALRRQKRLSPHSARARVTIEWLRFRDDGSNRT
                     ALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPGTWSSHKL
                     VRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEAPVTEGTRCCRQEMYLDLQG
                     MKWAENWILEPPGFLTYECVGSCLQLPESLTSRWPFLGPRQCVASEMTSLPMIVSVKE
                     GGRTRPQVVSLPNMRVQTCSCASDGALIPRRLQP"
     exon            339..585
                     /gene="Lefty1"
                     /gene_synonym="Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            586..831
                     /gene="Lefty1"
                     /gene_synonym="Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4"
                     /inference="alignment:Splign:2.1.0"
     exon            832..1621
                     /gene="Lefty1"
                     /gene_synonym="Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1599..1604
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Lefty1"
                     /gene_synonym="Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4"
                     /note="hexamer: AATAAA"
     polyA_site      1621
                     /gene="Lefty1"
                     /gene_synonym="Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4"
                     /note="major polyA site"
ORIGIN      
        1 gtaccgtacc attcctccgc agactcaaga ccctttcagg acacctcagg gacacacaca
       61 tccaaggctc ctcttcccgg acagcaccat gccattcctg tggctctgct gggcactctg
      121 ggcactgtcg ctggttagcc tcagggaagc cctgaccgga gagcagatcc tgggcagcct
      181 gctgcaacag ctgcagctcg atcaaccgcc agtcctggac aaggctgatg tggaagggat
      241 ggtcatcccc tcgcacgtga ggactcagta tgtggccctg ctacaacaca gccatgccag
      301 ccgctcccga ggcaagaggt tcagccagaa ccttcgagag gtggcaggca ggttcctggt
      361 gtcagagacc tccactcacc tgctagtgtt cggaatggag cagcggctgc cgcctaacag
      421 cgagctggtg caggctgtgc tgcggctgtt ccaggagcct gtgcccagaa cagctctccg
      481 gaggcaaaag aggctgtccc cacacagtgc ccgggctcgg gtcaccattg aatggctgcg
      541 cttccgcgac gacggctcca accgcactgc ccttatcgat tctaggctcg tgtccatcca
      601 cgagagcggc tggaaggcct tcgacgtgac cgaggccgtg aacttctggc agcagctgag
      661 ccggccgagg cagccgctgc tgctccaggt gtcggtgcag agggagcatc tggggccggg
      721 aacctggagc tcacacaagt tggttcgttt cgcggcgcag gggacgccgg atggcaaggg
      781 gcagggcgag ccacagctgg agctgcacac gctggacctc aaggactatg gagctcaagg
      841 caattgtgac cccgaggcac cagtgactga aggcacccga tgctgtcgcc aggagatgta
      901 cctggacctg caggggatga agtgggccga gaactggatc ctagaaccgc cagggttcct
      961 gacatatgaa tgtgtgggca gctgcctgca gctaccggag tccctgacca gcaggtggcc
     1021 atttctgggg cctcggcagt gtgtcgcctc agagatgacc tccctgccca tgattgtcag
     1081 cgtgaaggag ggaggcagga ccaggcctca agtggtcagc ctgcccaaca tgagggtgca
     1141 gacctgtagc tgcgcctcag atggggcgct catacccagg aggctgcagc cataggcgcg
     1201 gggtgtggct tccccaagga tgtgcctttc atgcaaatct gaagtgctca ttatactggg
     1261 agagctgggg attctaactc cctaatgggc aatccctgtg tgtgctcttt gcttcctctg
     1321 aagtagcctc atccctaaat ttttaccttc gaggaatgtg actcgctggc ccctggaggc
     1381 gctctgaccc agtggtctct gtccttcata ttgttcactg cactgtatgc gaagcactta
     1441 catgtataga tactgcaaac caaggacaga atccccaatt gccattgttc ccttaatttg
     1501 tcgctgaatc tgggctgagt cccagtcttg actctggacc taagccacaa gttgggcaaa
     1561 catgtccaac ctaggcaata ctggctttgc tagatgtgaa taaaatatgc tttgttttgt
     1621 aaaaaaaaaa aaaaaaaaa
//
