LOCUS       NM_008492               1338 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 1,
            mRNA.
ACCESSION   NM_008492
VERSION     NM_008492.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Spate,E., Zhou,B., Sun,T., Kusch,K., Asadollahi,E., Siems,S.B.,
            Depp,C., Werner,H.B., Saher,G., Hirrlinger,J., Mobius,W., Nave,K.A.
            and Goebbels,S.
  TITLE     Downregulated expression of lactate dehydrogenase in adult
            oligodendrocytes and its implication for the transfer of glycolysis
            products to axons
  JOURNAL   Glia 72 (8), 1374-1391 (2024)
   PUBMED   38587131
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Shi,F., Zhang,G., Li,J., Shu,L., Yu,C., Ren,D., Zhang,Y. and
            Zheng,P.
  TITLE     Integrated analysis of single cell-RNA sequencing and Mendelian
            randomization identifies lactate dehydrogenase B as a target of
            melatonin in ischemic stroke
  JOURNAL   CNS Neurosci Ther 30 (5), e14741 (2024)
   PUBMED   38702940
  REMARK    GeneRIF: Integrated analysis of single cell-RNA sequencing and
            Mendelian randomization identifies lactate dehydrogenase B as a
            target of melatonin in ischemic stroke.
REFERENCE   3  (bases 1 to 1338)
  AUTHORS   Cuozzo,F., Viloria,K., Shilleh,A.H., Nasteska,D., Frazer-Morris,C.,
            Tong,J., Jiao,Z., Boufersaoui,A., Marzullo,B., Rosoff,D.B.,
            Smith,H.R., Bonner,C., Kerr-Conte,J., Pattou,F., Nano,R.,
            Piemonti,L., Johnson,P.R.V., Spiers,R., Roberts,J., Lavery,G.G.,
            Clark,A., Ceresa,C.D.L., Ray,D.W., Hodson,L., Davies,A.P.,
            Rutter,G.A., Oshima,M., Scharfmann,R., Merrins,M.J., Akerman,I.,
            Tennant,D.A., Ludwig,C. and Hodson,D.J.
  TITLE     LDHB contributes to the regulation of lactate levels and basal
            insulin secretion in human pancreatic beta cells
  JOURNAL   Cell Rep 43 (4), 114047 (2024)
   PUBMED   38607916
  REMARK    GeneRIF: LDHB contributes to the regulation of lactate levels and
            basal insulin secretion in human pancreatic beta cells.
REFERENCE   4  (bases 1 to 1338)
  AUTHORS   Stiebler,A.C., Freitag,J., Schink,K.O., Stehlik,T., Tillmann,B.A.,
            Ast,J. and Bolker,M.
  TITLE     Ribosomal readthrough at a short UGA stop codon context triggers
            dual localization of metabolic enzymes in Fungi and animals
  JOURNAL   PLoS Genet 10 (10), e1004685 (2014)
   PUBMED   25340584
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1338)
  AUTHORS   Schueren,F., Lingner,T., George,R., Hofhuis,J., Dickel,C.,
            Gartner,J. and Thoms,S.
  TITLE     Peroxisomal lactate dehydrogenase is generated by translational
            readthrough in mammals
  JOURNAL   Elife 3, e03640 (2014)
   PUBMED   25247702
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1338)
  AUTHORS   Beier,H. and Grimm,M.
  TITLE     Misreading of termination codons in eukaryotes by natural nonsense
            suppressor tRNAs
  JOURNAL   Nucleic Acids Res 29 (23), 4767-4782 (2001)
   PUBMED   11726686
  REMARK    Review article
REFERENCE   7  (bases 1 to 1338)
  AUTHORS   Thomas,K., Del Mazo,J., Eversole,P., Bellve,A., Hiraoka,Y., Li,S.S.
            and Simon,M.
  TITLE     Developmental regulation of expression of the lactate dehydrogenase
            (LDH) multigene family during mouse spermatogenesis
  JOURNAL   Development 109 (2), 483-493 (1990)
   PUBMED   2401207
REFERENCE   8  (bases 1 to 1338)
  AUTHORS   Hiraoka,B.Y., Sharief,F.S., Yang,Y.W., Li,W.H. and Li,S.S.
  TITLE     The cDNA and protein sequences of mouse lactate dehydrogenase B.
            Molecular evolution of vertebrate lactate dehydrogenase genes A
            (muscle), B (heart) and C (testis)
  JOURNAL   Eur J Biochem 189 (2), 215-220 (1990)
   PUBMED   2338075
REFERENCE   9  (bases 1 to 1338)
  AUTHORS   Roller,R.J., Kinloch,R.A., Hiraoka,B.Y., Li,S.S. and Wassarman,P.M.
  TITLE     Gene expression during mammalian oogenesis and early embryogenesis:
            quantification of three messenger RNAs abundant in fully grown
            mouse oocytes
  JOURNAL   Development 106 (2), 251-261 (1989)
   PUBMED   2591314
REFERENCE   10 (bases 1 to 1338)
  AUTHORS   Britton-Davidian,J., Ruiz Bustos,A., Thaler,L. and Topal,M.
  TITLE     Lactate dehydrogenase polymorphism in Mus musculus L. and Mus
            spretus Lataste
  JOURNAL   Experientia 34 (9), 1144-1145 (1978)
   PUBMED   720506
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK019391.1 and BC046755.1.
            
            On Nov 13, 2014 this sequence version replaced NM_008492.2.
            
            Summary: This gene encodes the B subunit of lactate dehydrogenase
            enzyme, which catalyzes the interconversion of pyruvate and lactate
            with concomitant interconversion of NADH and NAD+ in a
            post-glycolysis process. Alternatively spliced transcript variants
            have also been found for this gene. Recent studies have shown that
            a C-terminally extended isoform is produced by use of an
            alternative in-frame translation termination codon via a stop codon
            readthrough mechanism, and that this isoform is localized in the
            peroxisomes. Pseudogenes have been identified on chromosomes 1 and
            19. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) encodes two isoforms, which
            result from the use of alternative in-frame translation termination
            codons. The shorter isoform (Ldhb) results from translation
            termination at the upstream UGA stop codon, while the longer
            isoform (Ldhbx) results from UGA stop codon readthrough to the
            downstream UAG termination codon. This RefSeq represents the
            shorter isoform (Ldhb), which is localized in the cytosol.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046755.1, AK019391.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AK019391.1         2-15
            15-1338             BC046755.1         1-1324
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 74.17 cM"
     gene            1..1338
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /note="lactate dehydrogenase B"
                     /db_xref="GeneID:16832"
                     /db_xref="MGI:MGI:96763"
     exon            1..105
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            106..240
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..1116
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /EC_number="1.1.1.27"
                     /note="isoform Ldhb is encoded by transcript variant 1;
                     L-lactate dehydrogenase B chain; LDH heart subunit;
                     lactate dehydrogenase 2, B chain"
                     /codon_start=1
                     /product="L-lactate dehydrogenase B chain isoform Ldhb"
                     /protein_id="NP_032518.1"
                     /db_xref="CCDS:CCDS20684.1"
                     /db_xref="GeneID:16832"
                     /db_xref="MGI:MGI:96763"
                     /translation="MATLKEKLIASVADDEAAVPNNKITVVGVGQVGMACAISILGKS
                     LADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQ
                     EGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVI
                     GSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPE
                     MGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVST
                     MVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL
                     "
     exon            241..358
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            359..532
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            533..706
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            707..824
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            825..948
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1304
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1281..1286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /note="hexamer: AATAAA"
     polyA_site      1304
                     /gene="Ldhb"
                     /gene_synonym="H-Ldh; Ldh-2; LDH-B; LDH-H; Ldh2"
                     /note="major polyA site"
ORIGIN      
        1 ctcctccttc ttgtagagcc ggagtctgcg atccgcagag cagcagcctg ctgactttgc
       61 agtggctccc ctgcctcagc gcgccgcaga gcctcctctt ttctggacaa gatggcaacc
      121 cttaaggaga agctcattgc gtccgttgca gatgatgagg ctgccgtccc gaacaacaag
      181 atcactgtag tgggcgttgg acaagtgggt atggcatgtg ccatcagcat tctgggaaag
      241 tctctggctg atgaacttgc cctggtggat gtgttggaag acaagctcaa aggagagatg
      301 atggacctgc agcacgggag cttgttcctc cagactccga aaattgtggc cgataaagat
      361 tactctgtga cagccaactc taagattgtg gtggtgacgg caggagtccg ccagcaggag
      421 ggggagagtc ggctcaacct ggtgcagaga aatgtcaacg tgttcaagtt catcattcct
      481 cagatcgtca agtacagccc tgactgcacc atcatcgtgg tttccaaccc agtggatatt
      541 ctgacttacg tcacctggaa actgagcggg ctacctaagc accgtgtgat tggaagcgga
      601 tgcaatctgg attctgctcg attccgctac ctcatggcag agaagcttgg cattcatccc
      661 agcagctgcc acggatggat cctgggcgag catggagact ccagtgtggc tgtgtggagc
      721 ggggtgaatg tggcaggagt ctccctccag gaactgaatc cagaaatggg gacagacaat
      781 gacagtgaga actggaagga ggtgcataag atggtggtgg acagtgccta tgaagtcatc
      841 aagctcaaag gctacaccaa ctgggccatc ggcctgagcg tggctgacct catcgagtcc
      901 atgctgaaaa acctctcccg gattcacccc gtgtctacca tggtgaaggg aatgtacggc
      961 attgagaatg aagtcttcct cagtctcccg tgcatcctca atgctcgggg gctgaccagc
     1021 gtcatcaatc agaagctgaa ggacgatgag gtcgctcagc tcaggaagag tgcggacacc
     1081 ctgtgggaca tccagaaaga cctcaaagac ctgtgactgc cagtctctag gctgtagaac
     1141 acaaacctcc aatgtgacca tgaaccttta gtcttcagcc atgtatgtag gtcacagttt
     1201 gcttcttccc tgacatgtga tatgagctca cagatcaaag cccaggcttg tttgatgttt
     1261 gcactaggag ctcctgatca aataaagtta gcaattgcag cataaaaaaa aaaaaaaaaa
     1321 aaaaaaaaaa aaaaaaaa
//
