LOCUS       NM_010699               1661 bp    mRNA    linear   ROD 06-NOV-2024
DEFINITION  Mus musculus lactate dehydrogenase A (Ldha), transcript variant 1,
            mRNA.
ACCESSION   NM_010699 XM_001002669
VERSION     NM_010699.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Li,H., Liu,C., Li,R., Zhou,L., Ran,Y., Yang,Q., Huang,H., Lu,H.,
            Song,H., Yang,B., Ru,H., Lin,S. and Zhang,L.
  TITLE     AARS1 and AARS2 sense L-lactate to regulate cGAS as global lysine
            lactyltransferases
  JOURNAL   Nature 634 (8036), 1229-1237 (2024)
   PUBMED   39322678
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Galvan,C., Flores,A.A., Cerrilos,V., Avila,I., Murphy,C., Zheng,W.,
            Christofk,H.R. and Lowry,W.E.
  TITLE     Defining metabolic flexibility in hair follicle stem cell induced
            squamous cell carcinoma
  JOURNAL   Sci Adv 10 (38), eadn2806 (2024)
   PUBMED   39303037
REFERENCE   3  (bases 1 to 1661)
  AUTHORS   Pioli,P.A., Hamilton,B.J., Connolly,J.E., Brewer,G. and Rigby,W.F.
  TITLE     Lactate dehydrogenase is an AU-rich element-binding protein that
            directly interacts with AUF1
  JOURNAL   J Biol Chem 277 (38), 35738-35745 (2002)
   PUBMED   12107167
REFERENCE   4  (bases 1 to 1661)
  AUTHORS   Crawford,R.M., Budas,G.R., Jovanovic,S., Ranki,H.J., Wilson,T.J.,
            Davies,A.M. and Jovanovic,A.
  TITLE     M-LDH serves as a sarcolemmal K(ATP) channel subunit essential for
            cell protection against ischemia
  JOURNAL   EMBO J 21 (15), 3936-3948 (2002)
   PUBMED   12145195
  REMARK    GeneRIF: The muscle subunit of lactate dehydrogenase is an integral
            part of the sarcolemmal K(ATP) channel complex in vivo, where it
            couples the metabolic status of the cell with the K(ATP) channel
            activity essential for protection against ischemia.
REFERENCE   5  (bases 1 to 1661)
  AUTHORS   Pretsch,W., Chatterjee,B., Favor,J., Merkle,S. and Sandulache,R.
  TITLE     Molecular, genetic and biochemical characterization of lactate
            dehydrogenase-A enzyme activity mutations in Mus musculus
  JOURNAL   Mamm Genome 9 (2), 144-149 (1998)
   PUBMED   9457676
REFERENCE   6  (bases 1 to 1661)
  AUTHORS   Merkle,S., Favor,J., Graw,J., Hornhardt,S. and Pretsch,W.
  TITLE     Hereditary lactate dehydrogenase A-subunit deficiency as cause of
            early postimplantation death of homozygotes in Mus musculus
  JOURNAL   Genetics 131 (2), 413-421 (1992)
   PUBMED   1644279
REFERENCE   7  (bases 1 to 1661)
  AUTHORS   Li,S.S., Tiano,H.F., Fukasawa,K.M., Yagi,K., Shimizu,M.,
            Sharief,F.S., Nakashima,Y. and Pan,Y.E.
  TITLE     Protein structure and gene organization of mouse lactate
            dehydrogenase-A isozyme
  JOURNAL   Eur J Biochem 149 (2), 215-225 (1985)
   PUBMED   3996406
REFERENCE   8  (bases 1 to 1661)
  AUTHORS   Akai,K., Yagi,K., Tiano,H.F., Pan,Y.C., Shimizu,M., Fong,K.,
            Jungmann,R.A. and Li,S.S.
  TITLE     Isolation and characterization of a cDNA and a pseudogene for mouse
            lactate dehydrogenase-A isozyme
  JOURNAL   Int J Biochem 17 (5), 645-648 (1985)
   PUBMED   2993055
REFERENCE   9  (bases 1 to 1661)
  AUTHORS   Lalley,P.A., Francke,U. and Minna,J.D.
  TITLE     Comparative gene mapping in man and mouse: assignment of the genes
            for lactate dehydrogenase-A, peptidase-D, and isocitrate
            dehydrogenase-2 to mouse chromosome 7
  JOURNAL   Cytogenet Cell Genet 22 (1-6), 577-580 (1978)
   PUBMED   752544
REFERENCE   10 (bases 1 to 1661)
  AUTHORS   O'Brien,D., Linnenbach,A. and Croce,C.M.
  TITLE     Assignment of the gene for lactic dehydrogenase A to mouse
            chromosome 7 using mouse-human hybrids
  JOURNAL   Cytogenet Cell Genet 21 (1-2), 72-76 (1978)
   PUBMED   648198
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC090123.68, BY227595.1 and
            BC094428.1.
            
            On Sep 10, 2009 this sequence version replaced NM_010699.1.
            
            Summary: The protein encoded by this gene catalyzes the conversion
            of L-lactate and NAD to pyruvate and NADH in the final step of
            anaerobic glycolysis. The protein is found predominantly in muscle
            tissue and belongs to the lactate dehydrogenase family. Mutations
            in this gene have been linked to hemolytic anemia and early
            postimplantation death in mice. Multiple transcript variants
            encoding different isoforms have been found for this gene. The
            mouse genome contains multiple pseudogenes of this gene. [provided
            by RefSeq, May 2013].
            
            Transcript Variant: This variant (1) encodes the predominant
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094428.1, AK168920.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC090123.68        131616-131618       c
            4-57                BY227595.1         4-57
            58-1661             BC094428.1         12-1615
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 30.6 cM"
     gene            1..1661
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /note="lactate dehydrogenase A"
                     /db_xref="GeneID:16828"
                     /db_xref="MGI:MGI:96759"
     exon            1..114
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /inference="alignment:Splign:2.1.0"
     exon            115..264
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..1137
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /EC_number="1.1.1.27"
                     /note="isoform 1 is encoded by transcript variant 1; LDH
                     muscle subunit; L-lactate dehydrogenase A chain; lactate
                     dehydrogenase A4; M-LDH; LDH-M; lactate dehydrogenase 1, A
                     chain"
                     /codon_start=1
                     /product="L-lactate dehydrogenase A chain isoform 1"
                     /protein_id="NP_034829.1"
                     /db_xref="CCDS:CCDS21289.1"
                     /db_xref="GeneID:16828"
                     /db_xref="MGI:MGI:96759"
                     /translation="MATLKDQLIVNLLKEEQAPQNKITVVGVGAVGMACAISILMKDL
                     ADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSSKDYCVTANSKLVIITAGARQQE
                     GESRLNLVQRNVNIFKFIIPNIVKYSPHCKLLIVSNPVDILTYVAWKISGFPKNRVIG
                     SGCNLDSARFRYLMGERLGVHALSCHGWVLGEHGDSSVPVWSGVNVAGVSLKSLNPEL
                     GTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPISTM
                     IKGLYGINEDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKSADTLWGIQKELQF"
     exon            265..382
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /inference="alignment:Splign:2.1.0"
     exon            383..556
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /inference="alignment:Splign:2.1.0"
     exon            557..730
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /inference="alignment:Splign:2.1.0"
     exon            731..848
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /inference="alignment:Splign:2.1.0"
     exon            849..972
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1623
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1604..1609
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /note="hexamer: AATAAA"
     polyA_site      1623
                     /gene="Ldha"
                     /gene_synonym="l7R2; Ldh1; Ldhm"
                     /note="major polyA site"
ORIGIN      
        1 ggagcttcca tttaaggccc cgcccgcgtg ctgctctgcg tgctggagcc actgtcgccg
       61 agctcgggcc acgctgcttc tcctcgccag tcgccccccc atcgtgcact agcggtctca
      121 aaagattcaa agtccaagat ggcaaccctc aaggaccagc tgattgtgaa tcttcttaag
      181 gaagagcagg ctccccagaa caagattaca gttgttgggg ttggtgctgt tggcatggct
      241 tgtgccatca gtatcttaat gaaggacttg gcggatgagc ttgcccttgt tgacgtcatg
      301 gaagacaaac tcaagggcga gatgatggat ctccagcatg gcagcctctt ccttaaaaca
      361 ccaaaaattg tctccagcaa agactactgt gtaactgcga actccaagct ggtcattatc
      421 accgcggggg cccgtcagca agagggggag agccggctca acctggtcca gcgaaacgtg
      481 aacatcttca agttcatcat tcccaacatt gtcaagtaca gtccacactg caagctgctg
      541 atcgtctcca atccagtgga tatcttgacc tacgtggctt ggaaaatcag tggctttccc
      601 aaaaaccgag taattggaag tggttgcaat ctggattcag cgcggttccg ttacctgatg
      661 ggagagaggc tgggggttca cgcgctgagc tgtcacggct gggtcctggg agaacatggc
      721 gactccagtg tgcctgtgtg gagtggtgtg aatgttgccg gcgtctccct gaagtctctt
      781 aacccagaac tgggcactga cgcagacaag gagcagtgga aggaggttca caagcaggtg
      841 gtggacagtg cctacgaggt gatcaagctg aaaggttaca catcctgggc cattggcctc
      901 tctgtggcag acttggctga gagcataatg aagaacctta ggcgggtgca tcccatttcc
      961 accatgatta agggtctcta tggaatcaat gaggatgtct tcctcagtgt cccatgtatc
     1021 ctgggacaaa atggaatctc ggatgttgtg aaggtgacac tgactcctga ggaagaggcc
     1081 cgcctgaaga agagcgcaga caccctctgg ggaatccaga aggagctgca gttctaaagt
     1141 cttccccgtg tcctagcact tcactgtcca ggctgcagca gggcttctag gcagaccaca
     1201 cccttctcgt ctgagctgtg gttagtacag tggtgttgag atggtgtggg gaaacatctc
     1261 actccccaca gctctgccct gctgccaagt ggtacttgtg tagtggtgac ctggttagtg
     1321 tgacagtccc actgtctctg agacacactg ccaactgcag gcttcgatta cccctgtgag
     1381 cctgctgcat tgctgccctg caccaaacat gcctaggccg acgagttccc agttaagtcg
     1441 tataacctgg ctccagtgtg tacgtccatg atgcatatct tgtgcataaa tgttgtacag
     1501 gatattttat atattatatg tgtctgtagt gtgcattgca atattatgtg agatgtaaga
     1561 tctgcatatg gatgatggaa ccaaccaccc aagtgtcatg ccaaataaaa ccttgaacag
     1621 tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//
