LOCUS       NM_001364261            2730 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus lipid droplet associated hydrolase (Ldah), transcript
            variant 5, mRNA.
ACCESSION   NM_001364261 XM_006515200
VERSION     NM_001364261.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Currall,B.B., Chen,M., Sallari,R.C., Cotter,M., Wong,K.E.,
            Robertson,N.G., Penney,K.L., Lunardi,A., Reschke,M., Hickox,A.E.,
            Yin,Y., Wong,G.T., Fung,J., Brown,K.K., Williamson,R.E.,
            Sinnott-Armstrong,N.A., Kammin,T., Ivanov,A., Zepeda-Mendoza,C.J.,
            Shen,J., Quade,B.J., Signoretti,S., Arnos,K.S., Banks,A.S.,
            Patsopoulos,N., Liberman,M.C., Kellis,M., Pandolfi,P.P. and
            Morton,C.C.
  TITLE     Loss of LDAH associated with prostate cancer and hearing loss
  JOURNAL   Hum Mol Genet 27 (24), 4194-4203 (2018)
   PUBMED   30169630
  REMARK    GeneRIF: We then amassed convergent genomic evidence showing
            population level associations between LDAHexpression and occurrence
            of prostate cancer . We further identified loss of LDAH in both
            tissues and cell lines derived from human prostate cancer, and
            generated Ldah-/- mouse model, which recapitulated many of the
            clinical findings in DGAP056.
            Erratum:[Hum Mol Genet. 2019 May 15;28(10):1753-1754. doi:
            10.1093/hmg/ddz036.. PMID: 31222336]
REFERENCE   3  (bases 1 to 2730)
  AUTHORS   Goo,Y.H., Son,S.H. and Paul,A.
  TITLE     Lipid Droplet-Associated Hydrolase Promotes Lipid Droplet Fusion
            and Enhances ATGL Degradation and Triglyceride Accumulation
  JOURNAL   Sci Rep 7 (1), 2743 (2017)
   PUBMED   28578400
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2730)
  AUTHORS   Kory,N., Grond,S., Kamat,S.S., Li,Z., Krahmer,N., Chitraju,C.,
            Zhou,P., Frohlich,F., Semova,I., Ejsing,C., Zechner,R.,
            Cravatt,B.F., Farese,R.V. Jr. and Walther,T.C.
  TITLE     Mice lacking lipid droplet-associated hydrolase, a gene linked to
            human prostate cancer, have normal cholesterol ester metabolism
  JOURNAL   J Lipid Res 58 (1), 226-235 (2017)
   PUBMED   27836991
  REMARK    GeneRIF: Our data suggest that LDAH is not a major cholesterol
            ester hydrolase, and an alternative metabolic function may be
            responsible for its possible effect on development of prostate
            cancer.
REFERENCE   5  (bases 1 to 2730)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 2730)
  AUTHORS   Goo,Y.H., Son,S.H., Kreienberg,P.B. and Paul,A.
  TITLE     Novel lipid droplet-associated serine hydrolase regulates
            macrophage cholesterol mobilization
  JOURNAL   Arterioscler Thromb Vasc Biol 34 (2), 386-396 (2014)
   PUBMED   24357060
  REMARK    GeneRIF: The data identify LDAH a candidate target to promote
            reverse cholesterol transport from atherosclerotic lesions.
REFERENCE   7  (bases 1 to 2730)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 2730)
  AUTHORS   Thiel,K., Heier,C., Haberl,V., Thul,P.J., Oberer,M., Lass,A.,
            Jackle,H. and Beller,M.
  TITLE     The evolutionarily conserved protein CG9186 is associated with
            lipid droplets, required for their positioning and for fat storage
  JOURNAL   J Cell Sci 126 (Pt 10), 2198-2212 (2013)
   PUBMED   23525007
  REMARK    GeneRIF: this study identified a novel lipid droplets -associated
            protein family, represented by the Drosophila protein CG9186 and
            its murine homolog MGI:1916082.
REFERENCE   9  (bases 1 to 2730)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   10 (bases 1 to 2730)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122860.5.
            
            On Jun 13, 2018 this sequence version replaced XM_006515200.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660821.95231.1,
                                           SRR11927937.5715963.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-79                AC122860.5         68679-68757
            80-235              AC122860.5         81153-81308
            236-379             AC122860.5         87773-87916
            380-552             AC122860.5         98996-99168
            553-787             AC122860.5         128917-129151
            788-870             AC122860.5         136361-136443
            871-2730            AC122860.5         144453-146312
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 3.85 cM"
     gene            1..2730
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /note="lipid droplet associated hydrolase"
                     /db_xref="GeneID:68832"
                     /db_xref="MGI:MGI:1916082"
     exon            1..79
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /inference="alignment:Splign:2.1.0"
     exon            80..235
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /inference="alignment:Splign:2.1.0"
     exon            236..379
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /inference="alignment:Splign:2.1.0"
     exon            380..552
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /inference="alignment:Splign:2.1.0"
     exon            553..787
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /inference="alignment:Splign:2.1.0"
     exon            788..870
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /inference="alignment:Splign:2.1.0"
     exon            871..2730
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2708..2713
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /note="hexamer: AATAAA"
     polyA_site      2730
                     /gene="Ldah"
                     /gene_synonym="1110057K04Rik; mLDAH"
                     /note="major polyA site"
ORIGIN      
        1 agtacaggcg gaacttccgg gtctggtgag gctttgccga gacgtcggcg gacgacccca
       61 ataccttgtg agtgggcaga gatggcctca gaagtcgagg aacaaatccc tgtgcgtgag
      121 gagttctttc tctgtggtgg agttgaaacc aagatcataa aatgtgggcc ctggactaac
      181 ctctttgaga aacaagacgt cagcaagcca aaacagctta tcttcatcat tcccggtaac
      241 ccaggctatt ccgcattcta cgtgccattt gcaaaggcct tgtacacttt gatgaagagc
      301 cgcttcccgg tatggataat ctctcatgct gggttttcgg tgactcccaa agacaagaag
      361 gttcttgcag caccacagga agagtcaaat gctcaaaaaa ttgaggacgt ctatggtctg
      421 aatggtcaaa tagagcacaa aatagctttt ctgagagctc acgtgccaaa ggatgtgaag
      481 cttatactca ttggccattc cgtaggcacc tatatgaccc ttcacgtgat gaagcgagtc
      541 cccgagctgc ctgtcgccca cgcctttctg ctcttcccaa ctatcgaacg aatgtctgaa
      601 tcgcccaacg gcaagtttgc cactccattt ttgtgccagt ttcgatacct gctctatgct
      661 accagctact tactttttaa gccgtgtcct gaagtcataa aatctttcat aatccaaaag
      721 ctcatgggac aaatgaacat taagcttgaa cttcctttga cggatatcct tcaaccattt
      781 tgccttgcta acgctgccta cctcgggagc caagaaatgg tccagatagt gaagcgagat
      841 gatgacatca taaaggagtt tctccctaag cttaagtttt actatggtaa aactgatggc
      901 tggtgtccag taaagtacta tgaagacatg aagaaagatt ttccagaagg caatatttac
      961 ctctgtgaga aaggaatacc acatgctttt gttctcgatt ttagccagga gatggctacc
     1021 atagttgctg agtggataaa taatagaccg cccagaaaat aggtactggc ccaggctgga
     1081 gagatggctc agtggttaag agtactgact gctcttccag aggtcctgag ttcgattccc
     1141 agcaaccaca tggtggctca caaccatctg taatgggatc cgatgccctc ttctggtgtg
     1201 tgtgaagaca gctacattaa cattaaaaaa aataataatt ctttaaaaaa aataagtact
     1261 ggcccaaggg gggaaaaagt cacctacagc caatgaaagt tctaggtgtg ttctgcatag
     1321 gggcaaatgt ttaatcgtaa tgcttaaaag aaaactagag accttcctgg cttacagatt
     1381 tccagctccc acaccctgcg ctaagactaa agtgaacccg gctgacagct cactccatgg
     1441 gggagcagtg agcaaaggat gctaacggtg cacttgccta gttgagggga aatcacttct
     1501 gacttcatac gggtccttgc tgagaccatc gccgtgatag gcgtgattca cctgcaataa
     1561 accaaacatc aagttggcct gacatttatc aactaactag acagagttgc tgaaggatcc
     1621 tgacttcatc ctgccacagg ataaccaaca ggctcctcct gagccctgaa aggccaggtc
     1681 cctgctggct cctctagagc aagaagggag ggctgcagtg tccacatcag atttgcacgg
     1741 gatttctcat cagtgtgatt cagctagtga cctccatgga caggatgcag tgagccaccc
     1801 attccattca tgatgccatt aactgccttg ggactcttcc cttctgaaaa tttcctagga
     1861 catcatcttt ggaaaggtgt tttaacttgt tgagtaaagc attagttcat tctccacttg
     1921 cctaaacttt attgattgga aagaaaggat tttgtgactg ttcagagttc tgaaagccat
     1981 taaatttgta tttcttcatt cccgaaagcc ttgaagtcca gtagaaaagc atattcctgg
     2041 ccgagacgcg gaggagtgct tccttcctga catactggac agctctgcta ccttaattga
     2101 ctcagtccta agatctcaga ggccaataaa cccaactgtc tatatgactt gaacagttgg
     2161 aagcattcca ttgtcttgta gagaaaagct ctcctagcag tatcattcgt tcctcaaggg
     2221 cagttgagac gcccttcctc tcaaacccct aaggccctga tgacggattt ggttctggtg
     2281 tgctgcctct caggggtgag aggaataggt tctaatcaca ctcgctcact ccgctcattc
     2341 tgtatggttt ctacagagga gctcagcctc tcagcgggga taagccaggc tccagacaag
     2401 gagccttagt ctaggaagac aacacgaggt cctccctggt gtctgcacaa ggatgcctcc
     2461 tgcagagctg atctgcgaag ccaggcttca ggctctgagt accttgctta tagagcaggc
     2521 ttggtcactg gtgggaatca atgcataaga acaagtcctg gcaagtcaga cctattggaa
     2581 caaaggaata gcatttgtgg atgtctctta caaattattt tacatcactc tttgttttcc
     2641 aaatcctgct taaaaggccc tggttcctca ttcctgttcc tcgcctcaac gtcaaatgat
     2701 attttataat aaatgtctcc cccgcagtca
//
